LOCUS       sequence-486238-        7398 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Expresses SARS-CoV-2 spike (Gamma) protein for creating pseudotyped 
            virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Youssef N, Gurev S, Ghantous F, Brock KP, Jaimes JA, Thadani NN, 
            Dauphin A, Sherman AC, Yurkovetskiy L, Soto D, Estanboulieh R, 
            Kotzen B, Notin P, Kollasch AW, Cohen AA, Dross SE, Erasmus J, 
            Fuller DH, Bjorkman PJ, Lemieux JE, Luban J, Seaman MS, Marks DS
  TITLE     Computationally designed proteins mimic antibody immune evasion in 
            viral evolution.
  JOURNAL   Immunity. 2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015.
  PUBMED    40345199
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2025 May 6:S1074-7613(25)00178-5. doi: 
            10.1016/j.immuni.2025.04.015."
COMMENT     Sequence Label: pDMJ2-SARS-CoV-2-Spike (Gamma)
FEATURES             Location/Qualifiers
     source          1..7398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    3794..4018
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4433..5021)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4513..4532)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5192..5983,5984..6052))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5815..5834
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6053..6157)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6340..6719
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6720..6923
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6874..6894
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6920..6944
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgttcgtgt ttctggttct tcttcccctg gtgtctagtc agtgcgtcaa cttcactaac
       61 cgaacacagc tgccctcagc ctacaccaat tcctttacac gaggcgtcta ctatcccgac
      121 aaggtgttcc gaagcagtgt tttgcactcc acgcaggacc ttttcttgcc cttcttctca
      181 aatgtgactt ggtttcatgc aatacacgta tccgggacta atggcactaa gagattcgac
      241 aatcccgtgt tgccttttaa cgacggcgtg tattttgcca gtacagaaaa gagcaatatt
      301 atccggggat ggatcttcgg cacgactctg gatagtaaaa cacaatccct gctgatcgtg
      361 aataatgcga caaacgtcgt cataaaagtc tgcgagttcc agttctgcaa ctaccctttc
      421 ctcggggtgt attaccataa gaacaataaa tcttggatgg aaagtgagtt tcgcgtgtac
      481 tccagtgcaa ataactgtac ttttgagtac gtaagccagc ctttcctgat ggacctcgaa
      541 gggaaacagg ggaactttaa gaacctgagc gaattcgtgt tcaagaacat cgacggttat
      601 tttaaaatat acagcaagca cacccctatt aacctggtga gagatttgcc acaaggtttt
      661 agcgccctgg aacccctcgt ggaccttcct atcggcatca acatcacgcg cttccaaact
      721 ctgctcgcac tgcatcggtc ttatctgacc cctggagatt catcctcagg atggaccgcg
      781 ggtgctgctg catattatgt gggatatctg cagcctagga ccttcctgct caaatacaac
      841 gagaacggca ccatcactga cgctgttgat tgcgcgctgg acccattgtc tgaaaccaaa
      901 tgcacactga aaagcttcac agtcgagaag ggcatttacc agacgagcaa cttcagagtt
      961 cagcccacag agagcatcgt tcgcttcccc aacattacca atttgtgccc attcggagag
     1021 gtctttaacg caactagatt tgccagtgtt tacgcctgga acaggaaacg gatatccaat
     1081 tgcgtggccg attacagcgt actgtacaat agtgctagct tttcaacctt taaatgctac
     1141 ggtgtctccc ctacgaagct gaatgatctg tgctttacta atgtctatgc ggacagcttc
     1201 gttattagag gtgacgaggt ccgccaaatc gccccagggc agactggaac gatagccgac
     1261 tacaattaca aactgccaga tgatttcacc ggctgcgtca tcgcctggaa ctccaataac
     1321 ctcgatagta aagtgggcgg caattacaac tatctctata ggctgtttcg aaagagcaac
     1381 ctgaaacctt ttgagcggga catttcaaca gagatctacc aggccggaag caccccctgc
     1441 aacggggtca aggggttcaa ttgttacttc ccgctgcaaa gctatggatt tcagccgact
     1501 tacggcgttg gctatcagcc atatcgggtg gtggtgctgt catttgagct gcttcatgcg
     1561 cctgctacag tatgtgggcc taaaaagtct accaatctgg tgaaaaataa gtgcgtgaac
     1621 ttcaacttca atggcctcac cgggacaggc gtgttgacag aatccaataa gaaattcctg
     1681 ccattccagc agttcggcag ggatatcgca gacacgaccg atgcggtgcg agatccccag
     1741 acattggaga ttctggacat tactccttgt tcttttggag gagtgagtgt tattactccc
     1801 ggcacaaata cctcaaatca agtcgctgtg ctgtatcaag gtgtaaactg taccgaggtc
     1861 cccgtggcta ttcacgccga ccagctgacc ccaacatggc gggtatacag caccggttct
     1921 aatgtattcc agaccagagc cggctgcctc attggagccg agtatgtgaa caactcttat
     1981 gaatgtgata taccgattgg agcaggtatc tgcgcctctt atcagaccca gacaaatagc
     2041 cccagaaggg ctaggagcgt ggcctcccag tccataatcg cctatacaat gtcactgggg
     2101 gctgaaaatt cagtggcata cagtaacaac tctatcgcta tccccacaaa ttttaccatc
     2161 tctgtgacta cagagatcct gccagtgtct atgactaaaa cctccgtgga ctgtaccatg
     2221 tacatctgtg gtgatagcac cgaatgttcc aacctgctcc tgcagtacgg cagcttctgc
     2281 acacagctga atagggcact gacaggaatc gccgtcgaac aggataagaa cactcaggag
     2341 gtgtttgcgc aggttaagca gatttacaag acgccaccaa tcaaggactt cggtgggttc
     2401 aatttctctc aaattctgcc tgatccttct aagccatcta agcggagctt tatagaggat
     2461 ttgctcttta ataaggtgac cctcgccgat gctggattta ttaagcagta tggcgactgt
     2521 ttgggagaca ttgctgctcg cgatctgata tgtgcccaga aattcaacgg gctgaccgta
     2581 ctcccaccgc tgctcactga tgagatgatc gcacaatata ccagcgccct gctggccggc
     2641 actattacat ccggctggac gttcggagca ggagccgcac tgcaaatacc ttttgccatg
     2701 cagatggcat atcgcttcaa tgggatcggg gtgacccaga acgtcctgta cgaaaaccag
     2761 aaacttatcg ctaaccagtt caattccgcc attggtaaaa tccaggacag cttgagttcc
     2821 acagccagcg ccctcgggaa attgcaagat gtagtcaatc agaatgcaca agccctgaat
     2881 actctcgtca agcaactgtc atccaacttc ggtgctatat cctccgtgct gaatgatatc
     2941 cttagtagat tggataaggt ggaggcagaa gtccagatcg accggcttat tactggaagg
     3001 ctgcagagtc tgcagacgta cgtcacgcag cagcttatca gagccgccga gattagagca
     3061 tctgcgaatc tggctgctat aaagatgagt gaatgcgtgc tcgggcaatc aaagcgggtc
     3121 gacttttgtg ggaaggggta ccacctcatg agcttccccc aatccgcccc acatggcgtt
     3181 gtgttcctgc atgttactta cgttcccgct caagagaaga actttactac cgctccggcg
     3241 atctgccacg acggtaaggc acactttcca cgcgaaggcg tctttgttag caatggaaca
     3301 cactggtttg ttacccagag gaacttttat gagccccaga taatcacaac cgacaacaca
     3361 ttcgtatctg ggaactgtga cgtagttatt gggatcgtga acaacaccgt gtacgatcct
     3421 ctccaaccgg aacttgactc tttcaaggag gaactggata agtactttaa gaatcacacc
     3481 tcacccgatg ttgacctcgg cgacatttca ggaattaatg ctagcttcgt gaacatccag
     3541 aaagagattg atagacttaa cgaagtggcc aagaacctca acgaatcact tattgacctg
     3601 caggagctgg gaaaatatga gcagtacatt aaatggcctt ggtacatttg gctgggcttt
     3661 atagcaggcc tgatcgctat cgtcatggtg accatcatgc tttgttgtat gactagctgc
     3721 tgcagctgcc tcaagggctg ttgtagctgc ggctcttgct gtaaataatg atgatgatga
     3781 ggatccagat ctgctgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     3841 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     3901 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     3961 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatgggt
     4021 acccaggtgc tgaagaattg acccggttcc tcctgggcca gaaagaagca ggcacatccc
     4081 cttctctgtg acacaccctg tccacgcccc tggttcttag ttccagcccc actcatagga
     4141 cactcatagc tcaggagggc tccgccttca atcccacccg ctaaagtact tggagcggtc
     4201 tctccctccc tcatcagccc accaaaccaa acctagcctc caagagtggg aagaaattaa
     4261 agcaagatag gctattaagt gcagagggag agaaaatgcc tccaacatgt gaggaagtaa
     4321 tgagagaaat catagaattt taaggccatg atttaaggcc aatcggaaag aacatgtgag
     4381 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4441 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4501 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4561 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4621 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4681 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4741 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4801 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     4861 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     4921 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     4981 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5041 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5101 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5161 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5221 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5281 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5341 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5401 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5461 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5521 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5581 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5641 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5701 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5761 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     5821 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     5881 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     5941 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6001 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6061 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6121 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6181 ctgacgtccc aatgattgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     6241 aaataccgca tcagattggc tattggccat tgcatacgtt gtatccatat cataatatgt
     6301 acatttatat tggctcatgt ccaacattac cgccatgttg acattgatta ttgactagtt
     6361 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     6421 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     6481 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     6541 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     6601 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     6661 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     6721 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     6781 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     6841 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     6901 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     6961 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccat cggctcgcat
     7021 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt
     7081 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa
     7141 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg
     7201 gctctccacg ctttgcctga ccctgcttgc tcaactctag ttaacggtgg agggcagtgt
     7261 agtctgagca gtactcgttg ctgccgcgcg cgccaccaga cataatagct gacagactaa
     7321 cagactgttc ctttccatgg gtcttttctg cagtcaccgt cgtcgacacg tgtgatcaga
     7381 tatcgcggcc gctctaga
//
