LOCUS       sequence-486260-       11019 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Encodes human VAV1 and N-terminal EBFP2 tag .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Qi Y, Yu CH
  TITLE     PI(3,4,5)P3-mediated Cdc42 activation regulates macrophage podosome 
            assembly.
  JOURNAL   Cell Mol Life Sci. 2025 Mar 24;82(1):127. doi: 
            10.1007/s00018-025-05664-2.
  PUBMED    40126693
REFERENCE   2  (bases 1 to 11019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci."; date: "2025-03-24"; volume: "82(1):127. doi"; pages: " 
            10.1007/s00018-025-05664-2"
COMMENT     Sequence Label: pLenti-EBFP2-VAV1
FEATURES             Location/Qualifiers
     source          1..11019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2975
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2987..3007)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2992..3321
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3172..3307
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3331..3930
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3331..3350)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3827..3847
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        4006..4309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4310..4513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4464..4484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4510..4534
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4606..4615
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4612..4614,4615..4617,4618..5328)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(4657..4678)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    7916..8504
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7969..7989)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8387..8398)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8595..8828
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8900..9021
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8937..8956)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8991..9010
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9061..9196
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9216..9235)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9217..9235)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9245..9262)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9245..9261)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9254..9276)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9403..9858
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9490..9509)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9700..9721
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9884..9988
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9989..10057,10058..10849)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10207..10226)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaattcaaa attttatcga ccctgtggaa tgtgtgtcag
     3001 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3061 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3121 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     3181 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     3241 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     3301 ggaggcctag gcttttgcaa aaagcttacc atgaccgagt acaagcccac ggtgcgcctc
     3361 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     3421 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     3481 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     3541 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     3601 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     3661 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     3721 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     3781 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     3841 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     3901 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc
     3961 gaaaggagcg cacgacccca tgcatcgata agcttgggag ttccgcgtta cataacttac
     4021 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     4081 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     4141 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     4201 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     4261 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     4321 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     4381 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     4441 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     4501 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     4561 tgacctccat agaagacacc gactctagag ctagcgctac cggtcgccac catggtgagc
     4621 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     4681 aacggccaca agttcagcgt gaggggcgag ggcgagggcg atgccaccaa cggcaagctg
     4741 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     4801 accctgagcc acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     4861 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     4921 gacggcacct acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     4981 atcgagctga agggcgtcga cttcaaggag gacggcaaca tcctggggca caagctggag
     5041 tacaacttca acagccacaa catctatatc atggccgtca agcagaagaa cggcatcaag
     5101 gtgaacttca agatccgcca caacgtggag gacggcagcg tgcagctcgc cgaccactac
     5161 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacagcca ctacctgagc
     5221 acccagtccg tgctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     5281 ttccgcaccg ccgccgggat cactctcggc atggacgagc tgtacaagat ggagctgtgg
     5341 cgccaatgca cccactggct catccagtgc cgggtgctgc cgcccagcca ccgcgtgacc
     5401 tgggatgggg ctcaggtgtg tgaactggcc caggccctcc gggatggtgt ccttctgtgt
     5461 cagctgctta acaacctgct accccatgcc atcaacctgc gtgaggtcaa cctgcgcccc
     5521 cagatgtccc agttcctgtg ccttaagaac attagaacct tcctgtccac ctgctgtgag
     5581 aagttcggcc tcaagcggag cgagctcttc gaagcctttg acctcttcga tgtgcaggat
     5641 tttggcaagg tcatctacac cctgtctgct ctgtcctgga ccccgatcgc ccagaacagg
     5701 gggatcatgc ccttccccac cgaggaggag agtgtaggtg atgaagacat ctacagtggc
     5761 ctgtccgacc agatcgacga cacggtggag gaggatgagg acctgtatga ctgcgtggag
     5821 aatgaggagg cggaaggcga cgagatctat gaggacctca tgcgctcgga gcccgtgtcc
     5881 atgccgccca agatgacaga gtatgacaag cgctgctgct gcctgcggga gatccagcag
     5941 acggaggaga agtacactga cacgctgggc tccatccagc agcatttctt gaagcccctg
     6001 caacggttcc tgaaacctca agacattgag atcatcttta tcaacattga ggacctgctt
     6061 cgtgttcata ctcacttcct aaaggagatg aaggaagccc tgggcacccc tggcgcagcc
     6121 aatctctacc aggtcttcat caaatacaag gagaggttcc tcgtctatgg ccgctactgc
     6181 agccaggtgg agtcagccag caaacacctg gaccgtgtgg ccgcagcccg ggaggacgtg
     6241 cagatgaagc tggaggaatg ttctcagaga gccaacaacg ggaggttcac cctgcgggac
     6301 ctgctgatgg tgcctatgca gcgagttctc aaatatcacc tccttctcca ggagctggtg
     6361 aaacacacgc aggaggcgat ggagaaggag aacctgcggc tggccctgga tgccatgagg
     6421 gacctggctc agtgcgtgaa cgaggtcaag cgagacaacg agacactgcg acagatcacc
     6481 aatttccagc tgtccattga gaacctggac cagtctctgg ctcactatgg ccggcccaag
     6541 atcgacgggg aactcaagat cacctcggtg gaacggcgct ccaagatgga caggtatgcc
     6601 ttcctgctcg acaaagctct actcatctgt aagcgcaggg gagactccta tgacctcaag
     6661 gactttgtaa acctgcacag cttccaggtt cgggatgact cttcaggaga ccgagacaac
     6721 aagaagtgga gccacatgtt cctcctgatc gaggaccaag gtgcccaggg ctatgagctg
     6781 ttcttcaaga caagagaatt gaagaagaag tggatggagc agtttgagat ggccatctcc
     6841 aacatctatc cggagaatgc caccgccaac gggcatgact tccagatgtt ctcctttgag
     6901 gagaccacat cctgcaaggc ctgtcagatg ctgcttagag gtaccttcta tcagggctac
     6961 cgctgccatc ggtgccgggc atctgcacac aaggagtgtc tggggagggt ccctccatgt
     7021 ggccgacatg ggcaagattt cccaggaact atgaagaagg acaaactaca tcgcagggct
     7081 caggacaaaa agaggaatga gctgggtctg cccaagatgg aggtgtttca ggaatactac
     7141 gggcttcctc caccccctgg agccattgga ccctttctac ggctcaaccc tggagacatt
     7201 gtggagctca cgaaggctga ggctgaacag aactggtggg agggcagaaa tacatctact
     7261 aatgaaattg gctggtttcc ttgtaacagg gtgaagccct atgtccatgg ccctcctcag
     7321 gacctgtctg ttcatctctg gtacgcaggc cccatggagc gggcaggggc agagagcatc
     7381 ctggccaacc gctcggacgg gactttcttg gtgcggcaga gggtgaagga tgcagcagaa
     7441 tttgccatca gcattaaata taacgtcgag gtcaagcaca ttaaaatcat gacagcagaa
     7501 ggactgtacc ggatcacaga gaaaaaggct ttccgggggc ttacggagct ggtggagttt
     7561 taccagcaga actctctaaa ggattgcttc aagtctctgg acaccacctt gcagttcccc
     7621 ttcaaggagc ctgaaaagag aaccatcagc aggccagcag tgggaagcac aaagtatttt
     7681 ggcacagcca aagcccgcta tgacttctgc gcccgagacc gatcagagct gtcgctcaag
     7741 gagggtgaca tcatcaagat ccttaacaag aagggacagc aaggctggtg gcgaggggag
     7801 atctatggcc gggttggctg gttccctgcc aactacgtgg aggaagatta ttctgaatac
     7861 tgctccggac tcagatctcg agctcaagct tcctcgagca ccagaattcg tcgacaatca
     7921 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7981 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     8041 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     8101 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     8161 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     8221 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     8281 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     8341 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     8401 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     8461 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattcgag
     8521 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     8581 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     8641 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     8701 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     8761 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8821 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     8881 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     8941 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     9001 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     9061 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     9121 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     9181 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     9241 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     9301 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     9361 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     9421 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     9481 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     9541 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     9601 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     9661 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     9721 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     9781 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     9841 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     9901 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     9961 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
    10021 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
    10081 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
    10141 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
    10201 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
    10261 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
    10321 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
    10381 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
    10441 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
    10501 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
    10561 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
    10621 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    10681 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    10741 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    10801 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    10861 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    10921 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    10981 ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
