LOCUS       sequence-486357-       10647 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  MYCMutMBIIIa expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10647)
  TITLE     Myc-related plasmids
REFERENCE   2  (bases 1 to 10647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: M77B
FEATURES             Location/Qualifiers
     source          1..10647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             12..53
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1017..1043
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1287..1316
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        1706..1917
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             1942..2541
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1942..1961)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2438..2458
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(2564..3137,3138..3140,3141..3149)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2731..2748)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2958..2977
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3150..3896
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3916..4504
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3969..3989)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4387..4398)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4576..4809
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4835..4852)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4841..5065
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5171..5188)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5342..5930)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5422..5441)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6101..6892,6893..6961))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6724..6743
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6962..7066)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7141..7160)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7326..7705
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7707..7905
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7923..8103
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8150..8275
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8768..9001
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9186..9230
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9528..9645
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        10297..10646
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    10305..10323
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10341..10359
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10377..10395
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10413..10431
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10449..10467
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10485..10503
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10521..10539
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10585..10609
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gatccaccat gggtaagccc attccgaatc ccctgctcgg cctggactca acaggatcta
       61 tgcctttgaa tgtaagtttt actaacagaa attacgactt agattacgac agtgtgcagc
      121 cttatttcta ctgcgacgaa gaagaaaact tttatcaaca gcaacagcag tcagagctgc
      181 aacctccagc tcctagtgag gacatttgga agaaatttga actgctgcca actccacctt
      241 taagcccttc tcggcgaagt gggctgtgta gtccttccta tgtcgccgtt actcccttta
      301 gcttaagagg ggataacgac ggtgggggtg gcagcttttc aactgccgac cagcttgaga
      361 tggttacgga gctcttgggt ggcgatatgg tgaatcagtc atttatatgt gaccctgacg
      421 acgaaacatt tatcaagaac atcatcatac aggactgtat gtggtcaggc tttagcgcag
      481 cggcaaaact cgtctccgaa aaattagcct cctaccaggc tgcgcggaaa gactctgggt
      541 cacccaaccc cgcaaggggt cattccgtgt gctccacaag cagcctgtat ttgcaggatc
      601 tcagtgctgc tgcgtctgag tgtgccgacc cgtctgctgc cagtccatat ccagcaaacg
      661 acagctcttc cccaaagtcc tgcgcaagtc aagattcctc cgccttttcc ccttcaagcg
      721 actccttgct gagcagcaca gaaagttcac cgcagggctc tccagagcca ttggtgctgc
      781 acgaagaaac tcctccaacc accagctctg acagtgagga ggagcaggag gatgaggaag
      841 agatcgatgt tgtatctgtg gaaaaaaggc aggctccagg taaacgcagt gagagtggaa
      901 gcccttccgc cggggggcac tccaaacctc cccatagtcc gttagtctta aaacggtgtc
      961 acgtatccac acaccagcac aattatgccg cacccccgtc tacaaggaaa gattatcccg
     1021 ccgcaaaacg cgtgaagctc gactcagtta gggtactccg acagattagc aataatcgga
     1081 agtgtacaag cccccggagt tcagacacag aggaaaacgt caagcgcaga acgcacaatg
     1141 ttctggagcg gcaaaggaga aatgaattga agcgctcctt tttcgcactg cgggatcaga
     1201 tccccgagct ggagaataat gaaaaagcac caaaagtggt catactgaaa aaggctaccg
     1261 catacatctt atctgtgcag gcagaagaac agaagctgat ttccgaagag gatctcctgc
     1321 gaaaacgccg tgagcaactg aagcacaaac tcgaacagtt gcggaacagt tgtgcctagt
     1381 gacgtacggc ggccgcctcg actagggata acagggtaat tgtttgaatg aggcttcagt
     1441 actttacaga atcgttgcct gcacatcttg gaaacacttg ctgggattac ttcgacttct
     1501 taacccaaca gaaggctcga gcaaccagaa ttcgagcaat tatcttgttt actaaaactg
     1561 aataccttgc tatctctttg atacattttt acaaagctga attaaaatgg tataaattaa
     1621 atcacttttt tcaattgaca cgtacgcgtg atctaattct actaggtctt gaaaggagtg
     1681 ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     1741 ttggggggag gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg ggtaaactgg
     1801 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     1861 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggac
     1921 cggttgtaga gcgctgccac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     1981 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     2041 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     2101 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     2161 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     2221 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     2281 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggagt ctcgcccgac
     2341 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     2401 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     2461 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     2521 acccgcaagc ccggtgcctg aacgccgcca tcgataagga gcgcccctct ccctcccccc
     2581 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     2641 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     2701 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     2761 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     2821 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     2881 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     2941 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     3001 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     3061 tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     3121 aaaaacacga tgataatatg gccacaacca tgagcagact ggacaagagc aaagtcataa
     3181 actctgctct ggaattactc aatggagtcg gtatcgaagg cctgacgaca aggaaactcg
     3241 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc
     3301 tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccactcc tgccccctgg
     3361 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc
     3421 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg
     3481 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac
     3541 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc
     3601 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga
     3661 aacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg
     3721 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg
     3781 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg
     3841 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaagctc
     3901 tagagttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3961 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4021 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4081 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4141 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4201 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4261 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     4321 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4381 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4441 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     4501 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     4561 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     4621 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4681 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4741 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4801 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     4861 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     4921 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4981 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     5041 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagcca ttaatgaaaa
     5101 gaaccagcca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     5161 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5221 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5281 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5341 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5401 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5461 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5521 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5581 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5641 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5701 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5761 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     5821 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5881 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5941 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6001 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6061 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6121 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6181 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6241 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6301 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6361 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6421 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6481 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6541 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6601 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6661 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     6721 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     6781 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     6841 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     6901 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     6961 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7021 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7081 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     7141 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     7201 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     7261 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     7321 atattgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     7381 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     7441 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     7501 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     7561 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     7621 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     7681 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     7741 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     7801 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     7861 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta
     7921 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7981 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8041 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8101 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     8161 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     8221 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     8281 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     8341 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     8401 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     8461 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     8521 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     8581 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     8641 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     8701 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     8761 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     8821 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     8881 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     8941 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     9001 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     9061 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     9121 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     9181 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     9241 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     9301 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     9361 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     9421 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     9481 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     9541 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     9601 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     9661 gacagcagag atccagtttg gttaattaag gtaccgggag gtggtccctg cagttacgcc
     9721 aatgataacc cccgccagaa aaatcttagt agccttccct ttttgttttc cgtgccccaa
     9781 ctcggcggat tgactcggcc ccttccggaa acacccgaat caacttctag tcaaattatt
     9841 gttcacgccg caatgaccca cccctggccc gcgtctgtgg aactgacccc tggtgtacag
     9901 gagagttcgc tgctgaaagt ggtcccaaag gggtactagt ttttaagctc ccaactcccc
     9961 ctcccccagc gtctggagga ttccacaccc tcgcaccgca ggggcgagga agtgggcgga
    10021 gtccggtttt ggcgccagcc gctgaggctg ccaagcagaa aagccaccgc tgaggagact
    10081 ccggtcactg tcctcgcccc gcctccccct tccctcccct tggggaccac cgggcgccac
    10141 gccgcgaacg gtaagtgccg cggtcgtcgg cgcctccgcc ctccccctag ggccccaatt
    10201 cccagcgggc gcggcgcgcg gccccgcgcg cagctcccgg ctccctcccc cttcggatgt
    10261 ggcttgagct gtaggcgcgg aggctagcct agattcgagt ttactcccta tcagtgatag
    10321 agaacgtatg aagagtttac tccctatcag tgatagagaa cgtatgcaga ctttactccc
    10381 tatcagtgat agagaacgta taaggagttt actccctatc agtgatagag aacgtatgac
    10441 cagtttactc cctatcagtg atagagaacg tatctacagt ttactcccta tcagtgatag
    10501 agaacgtata tccagtttac tccctatcag tgatagagaa cgtatgtcga ggtaggcgtg
    10561 tacggtgggc gcctataaaa gcagagctcg tttagtgaac cgtcagatcg cctggagcaa
    10621 ttccacaaca cttttgtctt atacttg
//
