LOCUS       sequence-486364-       10647 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  MYCMutMBIV expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10647)
  TITLE     Myc-related plasmids
REFERENCE   2  (bases 1 to 10647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: M76B
FEATURES             Location/Qualifiers
     source          1..10647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..54
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1018..1044
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1288..1317
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        1707..1918
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             1943..2542
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1943..1962)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2439..2459
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(2565..3138,3139..3141,3142..3150)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2732..2749)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2959..2978
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3151..3897
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3917..4505
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3970..3990)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4388..4399)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4577..4810
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4836..4853)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4842..5066
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5172..5189)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5343..5931)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5423..5442)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6102..6893,6894..6962))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6725..6744
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6963..7067)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7142..7161)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7327..7706
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7708..7906
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7924..8104
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8151..8276
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8769..9002
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9187..9231
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9529..9646
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        10298..10647
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    10306..10324
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10342..10360
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10378..10396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10414..10432
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10450..10468
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10486..10504
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10522..10540
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10586..10610
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggatccacca tgggaaaacc tatcccaaat cccctgttgg gcctggactc tacaggctca
       61 atgcccttga atgtgagctt cacaaacagg aattacgatc tcgattatga ctccgtacag
      121 ccctactttt attgtgacga agaagagaat ttctatcagc agcagcagca gagcgaactc
      181 cagccccccg ctccttcaga agacatctgg aaaaaattcg aactcctgcc taccccaccc
      241 ctgtccccat cacgccgctc cgggttgtgc tccccatctt acgtggctgt tactccgttc
      301 agcttaagag gcgataatga tggcgggggg gggagtttta gtaccgccga ccagttggaa
      361 atggtcacag aactgctggg cggggacatg gtgaaccagt cattcatctg cgacccagac
      421 gacgagactt tcattaagaa tattattatt caagattgca tgtggagtgg attcagtgcc
      481 gcggccaaac ttgtgagcga gaagctggcc tcttaccaag ccgccagaaa ggacagcggg
      541 tctcctaacc cagcacgagg acacagcgtt tgtagcacct cctctctcta tctgcaggat
      601 ctctctgcag cagcctccga atgtattgat ccttccgtcg tctttcccta cccacttaac
      661 gacagttcct cccctaagag ctgtgccagt caggatagct ccgcatttag ccctagtagc
      721 gatagccttc tgtcctcaac tgagtcttca cctcagggta gtcccgaacc tttggtactg
      781 cacgaagaga caccccccac gactagtagt gattctgagg aggagcagga ggacgaggaa
      841 gaaatagatg tagtgtccgt ggagaagcgc caggcccctg gcaagcggag tgagagtggc
      901 agtccgtcag ccggcggcca ttccaaaccg ccccacagcc ccttggtact gaagcgctgt
      961 cacgtgagta ctcaccagca caactacgaa gatggcggca gtacagccgc gagctaccct
     1021 gcagccaaga gagtgaagct tgacagtgta agggtattga gacagatttc caataacagg
     1081 aagtgcacta gccccaggtc ctcagacacc gaggagaacg tcaaacgacg aacacacaac
     1141 gtcctggaac ggcagaggcg gaacgaactt aagagatcct tcttcgcact gagggatcag
     1201 attccagaac tggaaaacaa tgagaaagct cccaaggttg tgatactcaa gaaagccacg
     1261 gcatatatcc tgagcgttca ggcagaggag caaaaactga tcagcgagga ggacctcttg
     1321 cgcaagagac gcgagcagct gaaacacaaa cttgagcaac tgcggaatag ctgtgcatag
     1381 tgacgtacgg cggccgcctc gactagggat aacagggtaa ttgtttgaat gaggcttcag
     1441 tactttacag aatcgttgcc tgcacatctt ggaaacactt gctgggatta cttcgacttc
     1501 ttaacccaac agaaggctcg agcaaccaga attcgagcaa ttatcttgtt tactaaaact
     1561 gaataccttg ctatctcttt gatacatttt tacaaagctg aattaaaatg gtataaatta
     1621 aatcactttt ttcaattgac acgtacgcgt gatctaattc tactaggtct tgaaaggagt
     1681 gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     1741 gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg gggtaaactg
     1801 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     1861 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagga
     1921 ccggttgtag agcgctgcca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     1981 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     2041 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     2101 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     2161 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     2221 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     2281 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggag tctcgcccga
     2341 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     2401 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     2461 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     2521 gacccgcaag cccggtgcct gaacgccgcc atcgataagg agcgcccctc tccctccccc
     2581 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     2641 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     2701 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     2761 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     2821 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     2881 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     2941 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     3001 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     3061 gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     3121 gaaaaacacg atgataatat ggccacaacc atgagcagac tggacaagag caaagtcata
     3181 aactctgctc tggaattact caatggagtc ggtatcgaag gcctgacgac aaggaaactc
     3241 gctcaaaagc tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc
     3301 ctgctcgatg ccctgccaat cgagatgctg gacaggcatc atacccactc ctgccccctg
     3361 gaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc
     3421 ctctcacatc gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac
     3481 gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca
     3541 ctgtacgctc tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag
     3601 catcaagtag caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg
     3661 aaacaagcaa ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg
     3721 gaactaatca tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac
     3781 gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt
     3841 gatatgctgc ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaagct
     3901 ctagagttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3961 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     4021 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     4081 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4141 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     4201 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4261 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     4321 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4381 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4441 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4501 cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     4561 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     4621 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     4681 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     4741 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     4801 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     4861 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     4921 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4981 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     5041 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagcc attaatgaaa
     5101 agaaccagcc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5161 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5221 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5281 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5341 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5401 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5461 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5521 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5581 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     5641 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     5701 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     5761 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     5821 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     5881 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     5941 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6001 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6061 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6121 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6181 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6241 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6301 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6361 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6421 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6481 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6541 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6601 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     6661 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     6721 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6781 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     6841 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     6901 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6961 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7021 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7081 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     7141 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     7201 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     7261 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     7321 gatattgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     7381 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     7441 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     7501 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     7561 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     7621 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     7681 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     7741 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     7801 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     7861 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
     7921 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7981 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8041 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8101 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     8161 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     8221 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     8281 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     8341 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     8401 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     8461 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     8521 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     8581 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     8641 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     8701 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     8761 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     8821 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     8881 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     8941 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     9001 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     9061 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     9121 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     9181 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     9241 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     9301 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     9361 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     9421 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     9481 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     9541 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     9601 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     9661 ggacagcaga gatccagttt ggttaattaa ggtaccggga ggtggtccct gcagttacgc
     9721 caatgataac ccccgccaga aaaatcttag tagccttccc tttttgtttt ccgtgcccca
     9781 actcggcgga ttgactcggc cccttccgga aacacccgaa tcaacttcta gtcaaattat
     9841 tgttcacgcc gcaatgaccc acccctggcc cgcgtctgtg gaactgaccc ctggtgtaca
     9901 ggagagttcg ctgctgaaag tggtcccaaa ggggtactag tttttaagct cccaactccc
     9961 cctcccccag cgtctggagg attccacacc ctcgcaccgc aggggcgagg aagtgggcgg
    10021 agtccggttt tggcgccagc cgctgaggct gccaagcaga aaagccaccg ctgaggagac
    10081 tccggtcact gtcctcgccc cgcctccccc ttccctcccc ttggggacca ccgggcgcca
    10141 cgccgcgaac ggtaagtgcc gcggtcgtcg gcgcctccgc cctcccccta gggccccaat
    10201 tcccagcggg cgcggcgcgc ggccccgcgc gcagctcccg gctccctccc ccttcggatg
    10261 tggcttgagc tgtaggcgcg gaggctagcc tagattcgag tttactccct atcagtgata
    10321 gagaacgtat gaagagttta ctccctatca gtgatagaga acgtatgcag actttactcc
    10381 ctatcagtga tagagaacgt ataaggagtt tactccctat cagtgataga gaacgtatga
    10441 ccagtttact ccctatcagt gatagagaac gtatctacag tttactccct atcagtgata
    10501 gagaacgtat atccagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt
    10561 gtacggtggg cgcctataaa agcagagctc gtttagtgaa ccgtcagatc gcctggagca
    10621 attccacaac acttttgtct tatactt
//
