LOCUS       sequence-486368-       10994 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  MYC knockdown + rescue.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10994)
  TITLE     Myc-related plasmids
REFERENCE   2  (bases 1 to 10994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: M93
FEATURES             Location/Qualifiers
     source          1..10994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..54
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             982..1008
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1252..1281
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     ncRNA           1538..1552
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           1673..1687
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           1808..1822
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        2054..2265
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2290..2889
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2290..2309)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2786..2806
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(2912..3485,3486..3488,3489..3497)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3079..3096)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3306..3325
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3498..4244
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4264..4852
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4317..4337)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4735..4746)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4924..5157
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5183..5200)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5189..5413
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5519..5536)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5690..6278)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5770..5789)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6449..7240,7241..7309))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7072..7091
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7310..7414)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7489..7508)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7674..8053
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8055..8253
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8271..8451
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8498..8623
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9116..9349
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9534..9578
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9876..9993
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        10645..10994
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    10653..10671
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10689..10707
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10725..10743
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10761..10779
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10797..10815
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10833..10851
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10869..10887
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10933..10957
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggatccacca tggggaagcc tatcccaaat ccactgttgg gcttggacag taccggcagc
       61 ggaaaaaaaa aaaaatctgg atcaatgccg cttaacgtgt ctttcactaa ccgaaattac
      121 gatcttgact acgattccgt ccagccttat ttctattgtg acgaagagga aaacttttac
      181 cagcagcagc agcagagcga gcttcagcct ccagccccct ccgaagatat ctggaagaag
      241 ttcgagttgc tgcctacccc cccccttagt ccttctcgac ggtctggtct ctgttcccca
      301 tcctatgttg cagtgactcc attttcactc cgcggggaca atgacggtgg aggtggcagc
      361 ttctcaacgg cggatcagtt agagatggtc actgaactgc ttgggggcga tatggtgaac
      421 cagagtttca tttgcgaccc cgacgacgag acttttatca aaaatatcat tattcaggat
      481 tgcatgtggt ccggcttttc cgcagctgcg aagctcgtgt cagagaagtt agcttcttac
      541 caggccgcac ggaaggatag cggatctccg aacccggcta ggggtcactc tgtgtgttct
      601 accagctcat tgtaccttca ggaccttagc gctgccgcta gcgagtgcat tgacccttcc
      661 gtggtgtttc cataccccct taacgactca agttcaccca aaagttgcgc ctcccaggac
      721 tctagtgctt ttagtcctag tagtgacagt ttactctcct ccactgagag ctcagactca
      781 gaagaggaac aagaagacga ggaggagatc gatgttgtct ccgtcgagaa gcgccaagcg
      841 cccggcaaac gatccgaatc agggagccct agtgcaggtg gtcacagtaa accgcctcat
      901 tcacctctcg tcctgaaacg atgccatgta agtacacatc agcacaacta tgcggcaccg
      961 ccaagtacca gaaaggatta tcctgctgca aaaagagtca aactggattc agtccgcgtt
     1021 cttaggcaaa tctctaataa tcgcaagtgt acctcacccc ggtctagtga cacagaggag
     1081 aatgtcaaga gaaggactca caatgtgttg gaacggcagc gaagaaacga gcttaaacgc
     1141 agcttcttcg cattgcgaga tcaaattcct gagcttgaga acaacgagaa ggcaccgaaa
     1201 gtcgtaatat tgaaaaaagc aacagcttac atattgtcag tccaggcaga ggagcaaaag
     1261 cttatctctg aagaggacct ccttcgaaaa agacgggaac aactcaagca caagcttgag
     1321 cagttgagga actcttgtgc atagtgacgt acggcggccg cctcgactag ggataacagg
     1381 gtaattgttt gaatgaggct tcagtacttt acagaatcgt tgcctgcaca tcttggaaac
     1441 acttgctggg attacttcga cttcttaacc caacagaagg ctcgagaagg tatattgctg
     1501 ttgacagtga gcgaaaagat ttagccataa tgtaatagtg aagccacaga tgtattacat
     1561 tatggctaaa tctttctgcc tactgcctcg gacttcaagg ggctagaatt gaaggctcga
     1621 gaaggtatat tgctgttgac agtgagcgcg ggcataaaag aactttttta tagtgaagcc
     1681 acagatgtat aaaaaagttc ttttatgccc atgcctactg cctcggactt caaggggcta
     1741 gaattgaagg ctcgagaagg tatattgctg ttgacagtga gcgccaccta tgaacttgtt
     1801 tcaaatagtg aagccacaga tgtatttgaa acaagttcat aggtgatgcc tactgcctcg
     1861 gacttcaagg ggctagaatt cgagcaatta tcttgtttac taaaactgaa taccttgcta
     1921 tctctttgat acatttttac aaagctgaat taaaatggta taaattaaat cacttttttc
     1981 aattgacacg tacgcgtgat ctaattctac taggtcttga aaggagtggg aattggctcc
     2041 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     2101 gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     2161 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     2221 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggaccg gttgtagagc
     2281 gctgccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     2341 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     2401 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     2461 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     2521 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     2581 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     2641 cccaaggagc ccgcgtggtt cctggccacc gtcggagtct cgcccgacca ccagggcaag
     2701 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     2761 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     2821 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     2881 ggtgcctgaa cgccgccatc gataaggagc gcccctctcc ctcccccccc cctaacgtta
     2941 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     3001 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     3061 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     3121 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     3181 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     3241 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     3301 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     3361 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     3421 taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     3481 ataatatggc cacaaccatg agcagactgg acaagagcaa agtcataaac tctgctctgg
     3541 aattactcaa tggagtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg
     3601 gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc
     3661 tgccaatcga gatgctggac aggcatcata cccactcctg ccccctggaa ggcgagtcat
     3721 ggcaagactt tctgcggaac aacgccaagt cataccgctg tgctctcctc tcacatcgcg
     3781 acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa
     3841 atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt
     3901 ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa
     3961 aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaaa caagcaattg
     4021 agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat
     4081 gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt
     4141 ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat atgctgcctg
     4201 ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtaagctcta gagttaagtc
     4261 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4321 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4381 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4441 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4501 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4561 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4621 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     4681 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4741 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4801 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     4861 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     4921 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     4981 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5041 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5101 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     5161 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     5221 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     5281 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     5341 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     5401 gtgggctcta tggcttctga ggcggaaaga accagccatt aatgaaaaga accagccatt
     5461 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5521 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5581 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5641 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5701 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5761 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5821 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5881 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5941 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6001 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6061 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6121 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6181 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6241 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6301 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6361 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6421 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6481 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6541 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6601 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6661 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6721 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     6781 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6841 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6901 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     6961 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7021 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7081 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7141 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7201 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7261 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7321 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7381 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7441 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
     7501 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     7561 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     7621 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat attgacattg
     7681 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     7741 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     7801 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     7861 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     7921 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     7981 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     8041 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     8101 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     8161 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     8221 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
     8281 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8341 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8401 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     8461 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     8521 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     8581 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     8641 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     8701 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     8761 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     8821 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     8881 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     8941 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     9001 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     9061 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     9121 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     9181 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     9241 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     9301 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     9361 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     9421 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     9481 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     9541 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     9601 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     9661 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     9721 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     9781 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
     9841 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     9901 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     9961 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
    10021 ccagtttggt taattaaggt accgggaggt ggtccctgca gttacgccaa tgataacccc
    10081 cgccagaaaa atcttagtag ccttcccttt ttgttttccg tgccccaact cggcggattg
    10141 actcggcccc ttccggaaac acccgaatca acttctagtc aaattattgt tcacgccgca
    10201 atgacccacc cctggcccgc gtctgtggaa ctgacccctg gtgtacagga gagttcgctg
    10261 ctgaaagtgg tcccaaaggg gtactagttt ttaagctccc aactccccct cccccagcgt
    10321 ctggaggatt ccacaccctc gcaccgcagg ggcgaggaag tgggcggagt ccggttttgg
    10381 cgccagccgc tgaggctgcc aagcagaaaa gccaccgctg aggagactcc ggtcactgtc
    10441 ctcgccccgc ctcccccttc cctccccttg gggaccaccg ggcgccacgc cgcgaacggt
    10501 aagtgccgcg gtcgtcggcg cctccgccct ccccctaggg ccccaattcc cagcgggcgc
    10561 ggcgcgcggc cccgcgcgca gctcccggct ccctccccct tcggatgtgg cttgagctgt
    10621 aggcgcggag gctagcctag attcgagttt actccctatc agtgatagag aacgtatgaa
    10681 gagtttactc cctatcagtg atagagaacg tatgcagact ttactcccta tcagtgatag
    10741 agaacgtata aggagtttac tccctatcag tgatagagaa cgtatgacca gtttactccc
    10801 tatcagtgat agagaacgta tctacagttt actccctatc agtgatagag aacgtatatc
    10861 cagtttactc cctatcagtg atagagaacg tatgtcgagg taggcgtgta cggtgggcgc
    10921 ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc tggagcaatt ccacaacact
    10981 tttgtcttat actt
//
