LOCUS       sequence-486387-       10793 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  MYC knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10793)
  TITLE     Myc-related plasmids
REFERENCE   2  (bases 1 to 10793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: M79
FEATURES             Location/Qualifiers
     source          1..10793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..54
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1042..1068
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     ncRNA           1337..1351
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           1472..1486
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           1607..1621
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        1853..2064
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2089..2688
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2089..2108)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2585..2605
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(2711..3284,3285..3287,3288..3296)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2878..2895)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3105..3124
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3297..4043
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4063..4651
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4116..4136)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4534..4545)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4723..4956
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4982..4999)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4988..5212
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5318..5335)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5489..6077)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5569..5588)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6248..7039,7040..7108))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6871..6890
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7109..7213)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7288..7307)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7473..7852
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7854..8052
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8070..8250
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8297..8422
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8915..9148
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9333..9377
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9675..9792
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        10444..10793
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    10452..10470
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10488..10506
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10524..10542
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10560..10578
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10596..10614
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10632..10650
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10668..10686
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10732..10756
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggatccacca tggggaagcc tatcccaaat ccactgttgg gcttggacag taccggcagc
       61 ggaaaaaaaa aaaaatctgg atcaatgccg cttaacgtgt ctttcactaa ccgaaattac
      121 gatcttgact acgattccgt ccagccttat ttctattgtg acgaagagga aaacttttac
      181 cagcagcagc agcagagcga gcttcagcct ccagccccct ccgaagatat ctggaagaag
      241 ttcgagttgc tgcctacccc cccccttagt ccttctcgac ggtctggtct ctgttcccca
      301 tcctatgttg cagtgactcc attttcactc cgcggggaca atgacggtgg aggtggcagc
      361 ttctcaacgg cggatcagtt agagatggtc actgaactgc ttgggggcga tatggtgaac
      421 cagagtttca tttgcgaccc cgacgacgag acttttatca aaaatatcat tattcaggat
      481 tgcatgtggt ccggcttttc cgcagctgcg aagctcgtgt cagagaagtt agcttcttac
      541 caggccgcac ggaaggatag cggatctccg aacccggcta ggggtcactc tgtgtgttct
      601 accagctcat tgtaccttca ggaccttagc gctgccgcta gcgagtgcat tgacccttcc
      661 gtggtgtttc cataccccct taacgactca agttcaccca aaagttgcgc ctcccaggac
      721 tctagtgctt ttagtcctag tagtgacagt ttactctcct ccactgagag ctccccccag
      781 gggtctcctg aacccctggt cctgcatgag gagacccccc ctacaacttc ttcagacagc
      841 gaagaagaac aggaagatga agaagagatc gacgtggtgt ctgtggaaaa acgacaagcc
      901 cctgggaaaa gaagcgagtc tgggtccccg agtgccgggg ggcattccaa accaccccat
      961 tcacccctgg tcctcaagcg gtgccacgtg tccactcatc aacacaatta tgcggctccc
     1021 ccaagcacta ggaaggatta tcctgctgcc aaacgggtta agctggactc cgtccgagtc
     1081 ctgcggcaga ttagcaataa caggaaatgc acctctccca gaagctccga tactgaagaa
     1141 aactgacgta cggcggccgc ctcgactagg gataacaggg taattgtttg aatgaggctt
     1201 cagtacttta cagaatcgtt gcctgcacat cttggaaaca cttgctggga ttacttcgac
     1261 ttcttaaccc aacagaaggc tcgagaaggt atattgctgt tgacagtgag cgaaaagatt
     1321 tagccataat gtaatagtga agccacagat gtattacatt atggctaaat ctttctgcct
     1381 actgcctcgg acttcaaggg gctagaattg aaggctcgag aaggtatatt gctgttgaca
     1441 gtgagcgcgg gcataaaaga acttttttat agtgaagcca cagatgtata aaaaagttct
     1501 tttatgccca tgcctactgc ctcggacttc aaggggctag aattgaaggc tcgagaaggt
     1561 atattgctgt tgacagtgag cgccacctat gaacttgttt caaatagtga agccacagat
     1621 gtatttgaaa caagttcata ggtgatgcct actgcctcgg acttcaaggg gctagaattc
     1681 gagcaattat cttgtttact aaaactgaat accttgctat ctctttgata catttttaca
     1741 aagctgaatt aaaatggtat aaattaaatc acttttttca attgacacgt acgcgtgatc
     1801 taattctact aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag
     1861 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc
     1921 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     1981 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     2041 aacgggtttg ccgccagaac acaggaccgg ttgtagagcg ctgccaccat gaccgagtac
     2101 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     2161 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     2221 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     2281 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     2341 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     2401 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     2461 ctggccaccg tcggagtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     2521 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     2581 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     2641 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaac gccgccatcg
     2701 ataaggagcg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     2761 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     2821 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     2881 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     2941 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     3001 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     3061 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     3121 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     3181 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc
     3241 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatga
     3301 gcagactgga caagagcaaa gtcataaact ctgctctgga attactcaat ggagtcggta
     3361 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
     3421 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
     3481 ggcatcatac ccactcctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
     3541 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
     3601 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
     3661 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
     3721 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
     3781 ccaccgattc tatgccccca cttctgaaac aagcaattga gctgttcgac cggcagggag
     3841 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
     3901 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag
     3961 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
     4021 accttgacat gctccccggg taagctctag agttaagtcg acaatcaacc tctggattac
     4081 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4141 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4201 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     4261 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     4321 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     4381 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4441 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     4501 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4561 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     4621 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     4681 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     4741 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     4801 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4861 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4921 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     4981 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5041 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5101 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5161 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     5221 gcggaaagaa ccagccatta atgaaaagaa ccagccatta atgaatcggc caacgcgcgg
     5281 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5341 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5401 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5461 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5521 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5581 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5641 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5701 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5761 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5821 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5881 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     5941 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6001 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6061 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6121 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6181 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6241 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6301 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6361 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6421 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6481 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6541 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6601 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6661 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6721 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6781 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6841 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6901 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6961 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7021 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7081 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7141 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7201 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     7261 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     7321 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     7381 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     7441 tgcgctgctt cgcgatgtac gggccagata ttgacattga ttattgacta gttattaata
     7501 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     7561 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     7621 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     7681 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     7741 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     7801 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     7861 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     7921 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     7981 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     8041 ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     8101 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8161 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8221 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     8281 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     8341 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     8401 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     8461 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     8521 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     8581 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     8641 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     8701 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     8761 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     8821 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     8881 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     8941 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     9001 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     9061 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     9121 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     9181 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     9241 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     9301 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     9361 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     9421 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     9481 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     9541 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     9601 gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca
     9661 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     9721 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     9781 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt aattaaggta
     9841 ccgggaggtg gtccctgcag ttacgccaat gataaccccc gccagaaaaa tcttagtagc
     9901 cttccctttt tgttttccgt gccccaactc ggcggattga ctcggcccct tccggaaaca
     9961 cccgaatcaa cttctagtca aattattgtt cacgccgcaa tgacccaccc ctggcccgcg
    10021 tctgtggaac tgacccctgg tgtacaggag agttcgctgc tgaaagtggt cccaaagggg
    10081 tactagtttt taagctccca actccccctc ccccagcgtc tggaggattc cacaccctcg
    10141 caccgcaggg gcgaggaagt gggcggagtc cggttttggc gccagccgct gaggctgcca
    10201 agcagaaaag ccaccgctga ggagactccg gtcactgtcc tcgccccgcc tcccccttcc
    10261 ctccccttgg ggaccaccgg gcgccacgcc gcgaacggta agtgccgcgg tcgtcggcgc
    10321 ctccgccctc cccctagggc cccaattccc agcgggcgcg gcgcgcggcc ccgcgcgcag
    10381 ctcccggctc cctccccctt cggatgtggc ttgagctgta ggcgcggagg ctagcctaga
    10441 ttcgagttta ctccctatca gtgatagaga acgtatgaag agtttactcc ctatcagtga
    10501 tagagaacgt atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact
    10561 ccctatcagt gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat
    10621 ctacagttta ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga
    10681 tagagaacgt atgtcgaggt aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt
    10741 agtgaaccgt cagatcgcct ggagcaattc cacaacactt ttgtcttata ctt
//
