LOCUS       sequence-486392-       10367 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  MYC knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10367)
  TITLE     Myc-related plasmids
REFERENCE   2  (bases 1 to 10367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: M80
FEATURES             Location/Qualifiers
     source          1..10367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     ncRNA           911..925
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           1046..1060
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           1181..1195
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        1427..1638
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             1663..2262
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1663..1682)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2159..2179
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(2285..2858,2859..2861,2862..2870)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2452..2469)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2679..2698
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2871..3617
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3637..4225
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3690..3710)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4108..4119)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4297..4530
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4556..4573)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4562..4786
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4892..4909)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5063..5651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5143..5162)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5822..6613,6614..6682))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6445..6464
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6683..6787)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6862..6881)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7047..7426
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7428..7626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7644..7824
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7871..7996
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8489..8722
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8907..8951
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9249..9366
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        10018..10367
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    10026..10044
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10062..10080
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10098..10116
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10134..10152
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10170..10188
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10206..10224
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10242..10260
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10306..10330
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggatccacca tggtcagtaa gggggaggaa gacaatatgg cgattattaa ggagttcatg
       61 agatttaaag tgcacatgga aggctccgtc aacggtcatg aatttgaaat cgagggcgaa
      121 ggggaaggca gaccttacga agggactcag accgcaaaac tcaaggtgac caagggaggg
      181 cctcttcctt ttgcctggga cattctgagc ccccagttta tgtacggcag taaggcttac
      241 gtgaagcacc cagctgacat tccagactat ctgaagctca gtttcccaga gggattcaag
      301 tgggaaagag tgatgaattt cgaggacggt ggagtggtga ctgtaacaca agactcaagc
      361 ctgcaagacg gcgagttcat ttacaaagtt aaactccgag ggactaattt tccttccgat
      421 ggtccagtga tgcagaaaaa gacgatgggt tgggaagcct cttcagaaag aatgtaccca
      481 gaggacggcg ctctgaaagg agagatcaag cagagactca agctcaaaga tggtggccat
      541 tatgacgccg aggttaagac cacatacaaa gccaaaaaac cagtccagtt accaggagcc
      601 tacaatgtca atattaagct cgatatcaca tcccacaacg aggattatac cattgtggag
      661 caatatgaac gagctgaagg tcgtcatagt acaggaggta tggacgaact gtataaatga
      721 cgtacggcgg ccgcctcgac tagggataac agggtaattg tttgaatgag gcttcagtac
      781 tttacagaat cgttgcctgc acatcttgga aacacttgct gggattactt cgacttctta
      841 acccaacaga aggctcgaga aggtatattg ctgttgacag tgagcgaaaa gatttagcca
      901 taatgtaata gtgaagccac agatgtatta cattatggct aaatctttct gcctactgcc
      961 tcggacttca aggggctaga attgaaggct cgagaaggta tattgctgtt gacagtgagc
     1021 gcgggcataa aagaactttt ttatagtgaa gccacagatg tataaaaaag ttcttttatg
     1081 cccatgccta ctgcctcgga cttcaagggg ctagaattga aggctcgaga aggtatattg
     1141 ctgttgacag tgagcgccac ctatgaactt gtttcaaata gtgaagccac agatgtattt
     1201 gaaacaagtt cataggtgat gcctactgcc tcggacttca aggggctaga attcgagcaa
     1261 ttatcttgtt tactaaaact gaataccttg ctatctcttt gatacatttt tacaaagctg
     1321 aattaaaatg gtataaatta aatcactttt ttcaattgac acgtacgcgt gatctaattc
     1381 tactaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca
     1441 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgatccgg tgcctagaga
     1501 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     1561 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     1621 tttgccgcca gaacacagga ccggttgtag agcgctgcca ccatgaccga gtacaagccc
     1681 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     1741 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     1801 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     1861 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     1921 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     1981 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     2041 accgtcggag tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     2101 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     2161 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     2221 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgccgcc atcgataagg
     2281 agcgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     2341 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     2401 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     2461 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     2521 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     2581 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     2641 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     2701 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     2761 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca
     2821 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc atgagcagac
     2881 tggacaagag caaagtcata aactctgctc tggaattact caatggagtc ggtatcgaag
     2941 gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc ctgtactggc
     3001 acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg gacaggcatc
     3061 atacccactc ctgccccctg gaaggcgagt catggcaaga ctttctgcgg aacaacgcca
     3121 agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat ctcggcaccc
     3181 gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag
     3241 gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt acactgggct
     3301 gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca cctaccaccg
     3361 attctatgcc cccacttctg aaacaagcaa ttgagctgtt cgaccggcag ggagccgaac
     3421 ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag ctaaagtgcg
     3481 aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc ccagccgatg
     3541 cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat tttgaccttg
     3601 acatgctccc cgggtaagct ctagagttaa gtcgacaatc aacctctgga ttacaaaatt
     3661 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3721 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3781 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3841 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3901 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3961 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4021 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     4081 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     4141 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     4201 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     4261 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     4321 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     4381 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     4441 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     4501 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
     4561 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4621 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4681 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4741 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4801 agaaccagcc attaatgaaa agaaccagcc attaatgaat cggccaacgc gcggggagag
     4861 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4921 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4981 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5041 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5101 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5161 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5221 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5281 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5341 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     5401 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     5461 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     5521 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     5581 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     5641 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5701 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     5761 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5821 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5881 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5941 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6001 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6061 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6121 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6181 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6241 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6301 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6361 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6421 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6481 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6541 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6601 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6661 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6721 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6781 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     6841 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     6901 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     6961 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     7021 gcttcgcgat gtacgggcca gatattgaca ttgattattg actagttatt aatagtaatc
     7081 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     7141 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     7201 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     7261 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     7321 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     7381 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     7441 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     7501 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     7561 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     7621 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7681 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7741 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7801 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     7861 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     7921 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     7981 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     8041 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     8101 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     8161 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     8221 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     8281 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     8341 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     8401 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     8461 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     8521 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     8581 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     8641 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     8701 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     8761 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     8821 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     8881 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     8941 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     9001 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     9061 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     9121 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     9181 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     9241 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     9301 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     9361 aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa ggtaccggga
     9421 ggtggtccct gcagttacgc caatgataac ccccgccaga aaaatcttag tagccttccc
     9481 tttttgtttt ccgtgcccca actcggcgga ttgactcggc cccttccgga aacacccgaa
     9541 tcaacttcta gtcaaattat tgttcacgcc gcaatgaccc acccctggcc cgcgtctgtg
     9601 gaactgaccc ctggtgtaca ggagagttcg ctgctgaaag tggtcccaaa ggggtactag
     9661 tttttaagct cccaactccc cctcccccag cgtctggagg attccacacc ctcgcaccgc
     9721 aggggcgagg aagtgggcgg agtccggttt tggcgccagc cgctgaggct gccaagcaga
     9781 aaagccaccg ctgaggagac tccggtcact gtcctcgccc cgcctccccc ttccctcccc
     9841 ttggggacca ccgggcgcca cgccgcgaac ggtaagtgcc gcggtcgtcg gcgcctccgc
     9901 cctcccccta gggccccaat tcccagcggg cgcggcgcgc ggccccgcgc gcagctcccg
     9961 gctccctccc ccttcggatg tggcttgagc tgtaggcgcg gaggctagcc tagattcgag
    10021 tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca gtgatagaga
    10081 acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt tactccctat
    10141 cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac gtatctacag
    10201 tttactccct atcagtgata gagaacgtat atccagttta ctccctatca gtgatagaga
    10261 acgtatgtcg aggtaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa
    10321 ccgtcagatc gcctggagca attccacaac acttttgtct tatactt
//
