LOCUS       sequence-486424-       10202 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Dual luciferase (nano, firefly) with Zfp423 5' end fused to 
            NanoLuciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Concepcion D, Liang C, Kim D, Hamilton BA
  TITLE     Nonequivalence of Zfp423 premature termination codons in mice.
  JOURNAL   bioRxiv [Preprint]. 2025 Jun 2:2025.05.30.656936. doi: 
            10.1101/2025.05.30.656936.
  PUBMED    40501617
REFERENCE   2  (bases 1 to 10202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Jun 2:2025.05.30.656936. doi: 
            10.1101/2025.05.30.656936."
COMMENT     Sequence Label: pZNpF-wt
FEATURES             Location/Qualifiers
     source          1..10202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1046..1065
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1213..1812
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1213..1232)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1709..1729
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    1839..1887
                     /note="synthetic polyadenylation signal"
     CDS             join(2153..2221,2222..3013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2371..2390)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3026..3162
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3314..3385
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3390..3436)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3454..3502
                     /note="synthetic polyadenylation signal"
     promoter        3539..4717
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3770..4708
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4665..4685
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5260..5772
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     promoter        6015..6525
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6444..6462
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6622..8274
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    8324..8445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8335..8354)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8389..8408
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      8560..8606
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      8636..8772
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    8817..8888
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(9114..9702)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9194..9213)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(10202,1..1209)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcgtcacgg cgagcgctgc
       61 cacgtcagac gaagggcgca ggagcgtcct gatccttccg cccggacgct caggacagcg
      121 gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga
      181 cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa
      241 aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga
      301 ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg
      361 cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg
      421 ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca
      481 agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca
      541 gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg
      601 tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gagcccttcg
      661 ctaatgcggg aaagctctta ttcgggtgag atgggctggg caccatctgg ggaccctgac
      721 gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat
      781 ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg
      841 acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg
      901 taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga
      961 caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt
     1021 ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg
     1081 gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata
     1141 tgtaattttc agtgttagac ttgtaaattg tccgctaaat tctggccgtt tttggctttt
     1201 ttgttagaca acatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     1261 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     1321 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     1381 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     1441 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     1501 gagttgagcg gttcccggct ggccgcgcag caacagatgg agggcctcct ggcgccgcac
     1561 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     1621 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg ccgccgagcg cgccggggtg
     1681 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     1741 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     1801 cccggtgcct aggctagtat gtaagcctag tcttagataa taaaatcgct atccatcgaa
     1861 gatggatgtg tgttggtttt ttgtgtgtgt aacgctaggc gcgcctggtg taccgagaac
     1921 gatcctctca gtgcgagtct cgacgatcca tatcgttgct tggcagtcag ccagtcggaa
     1981 tccagcttgg gacccaggaa gtccaatcgt cagatattgt actcaagcct ggtcacggca
     2041 gcgtaccgat ctgtttaaac ctagatattg atagtctgat cggtcaacgt ataatcgagt
     2101 cctagctttt gcaaacatct atcaagagac aggatcagca ggaggctttc gcatgagtat
     2161 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2221 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cgcgagtggg
     2281 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2341 ctttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2401 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2461 ttcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2521 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga ttggaggacc
     2581 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2641 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2701 aatggcaaca accttgcgta aactattaac tggcgaacta cttactctag cttcccggca
     2761 acagttgata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2821 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2881 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2941 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3001 taagcattgg taaccgattc taggtgcatt ggcgcagaaa aaaatgcctg atgcgacgct
     3061 gcgcgtctta tactcccaca tatgccagat tcagcaacgg atacggcttc cccaacttgc
     3121 ccacttccat acgtgtcctc cttaccagaa atttatcctt aagatcccga atcgtttaaa
     3181 ctcgactctg gctctatcga atctccgtcg tttcgagctt acgcgaacag ccgtggcgct
     3241 catttgctcg tcgggcatcg aatctcgtca gctatcgtca gcttaccttt ttggcagcga
     3301 tcgcggctcc cgacatcttg gaccattagc tccacaggta tcttcttccc tctagtggtc
     3361 ataacagcag cttcagctac ctctcaattc aaaaaacccc tcaagacccg tttagaggcc
     3421 ccaaggggtt atgctatcaa tcgttgcgtt acacacacaa aaaaccaaca cacatccatc
     3481 ttcgatggat agcgatttta ttatctaact gctgatcgag tgtagccaga tctcgtgagg
     3541 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     3601 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     3661 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcact
     3721 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
     3781 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
     3841 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
     3901 gagttcgtgg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg tggcctggcc
     3961 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
     4021 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
     4081 agatagtctt gtaaatgcgg gccaagatca gcacactggt atttcggttt ttggggccgc
     4141 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     4201 gcggccaccg agaatcggac gggggtagtc tcaagctgcc cggcctgctc tggtgcctgg
     4261 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     4321 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagcacaa aatggaggac
     4381 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     4441 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     4501 gttctccagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     4561 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     4621 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     4681 ggttcaaagt ttttttcttc catttcaggt gtcgtgaaaa ctacccctaa aagccaaaag
     4741 atctttgtcg atcctaccat ccactcgaca cacccgccag cggccgctgc caagcttccg
     4801 agctctcgaa ttcagccgac cgcggacggc gacggagcgc ccggagcccc ggacatgtcc
     4861 aggcggaagc aggcgaagcc gcgatcggtg aaagttgaag agggggaggc ctcggacttc
     4921 tcgctggcct gggattcctc tgtggcagca gcaggaggcc tggaaggaga gccagagtgt
     4981 gatcggaaaa ccagccgtgc gctggaagac aggaacagcg tgacaagtca agaggagaga
     5041 aatgaggacg atgaagacgt ggaagatgag tcaatttaca cctgcgatca ctgtcagcag
     5101 gacttcgagt ctctggcaga cctgacggac caccgggccc accgctgtcc tggagatggt
     5161 gatgacgacc cacagctctc ctgggtggct tcatctccct ccagcaagga tgttgcgtca
     5221 cctacgcaga tgataggagg aggtggatcc ggaggcggag tcttcacact cgaagatttc
     5281 gttggggact ggcgacagac agccggctac aacctggacc aagtccttga acagggaggt
     5341 gtgtccagtt tgtttcagaa tctcggggtg tccgtaactc cgatccaaag gattgtcctg
     5401 agcggtgaaa atgggctgaa gatcgacatc catgtcatca tcccgtatga aggtctgagc
     5461 ggcgaccaaa tgggccagat cgaaaaaatt tttaaggtgg tgtaccctgt ggatgatcat
     5521 cactttaagg tgatcctgca ctatggcaca ctggtaatcg acggggttac gccgaacatg
     5581 atcgactatt tcggacggcc gtatgaaggc atcgccgtgt tcgacggcaa aaagatcact
     5641 gtaacaggga ccctgtggaa cggcaacaaa attatcgacg agcgcctgat caaccccgac
     5701 ggctccctgc tgttccgagt aaccatcaac ggagtgaccg gctggcggct gtgcgaacgc
     5761 attctggcgt aatattaaga aaccctggac cacccacccc agcaaggaca ctgagcaaga
     5821 gaggccctat cccaactcgg cccccaacac tgagcatctc cctcacaatt tccatcccag
     5881 acccccataa taacaggagg ggcccaggga gccctcccta ctctcttgaa taccatcaat
     5941 aaagttcgct gcacccacct ctccttggtt ttaatgcggt gttgggggag ggagggtgtg
     6001 taaataaatc tcgaggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag
     6061 ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc
     6121 acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc
     6181 ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc
     6241 cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag
     6301 ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg
     6361 cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg
     6421 tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg
     6481 gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccagctgac cacttcggtc
     6541 ctgagcgctt gggagttagg ttgtttgccg gcgtagcggc cagcgcctga gcccgccctt
     6601 gatcttcgct gtggcgtcga catggaagat gccaaaaaca ttaagaaggg cccagcgcca
     6661 ttctacccac tcgaagacgg gaccgccggc gagcaactgc acaaagccat gaagcgctac
     6721 gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac
     6781 gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat
     6841 acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg
     6901 ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag
     6961 ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg
     7021 caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat
     7081 agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca
     7141 cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc
     7201 ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc
     7261 accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc
     7321 gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg
     7381 ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc
     7441 ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac actctttagc
     7501 ttcttcgcta agagcactct gattgataag tacgacctaa gcaacttgca cgagatcgcc
     7561 agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta
     7621 ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc
     7681 gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg
     7741 gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt
     7801 ggccccatga tcatgagcgg ctacgttaac aacccagagg ctacaaacgc tctcatcgac
     7861 aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc
     7921 atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa
     7981 ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc
     8041 gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg
     8101 accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc
     8161 ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc
     8221 aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt gtaagctagc
     8281 ttgactgact gagatacagc gtaccttcag ctcacagaca tgataagata cattgatgag
     8341 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     8401 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     8461 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     8521 ctctacaaat gtggtattgg cccatctcta tcggtatcgt agcataaccc cttggggcct
     8581 ctaaacgggt cttgaggggt tttttgtgcc cctcgggccg gattgctatc taccggcatt
     8641 ggcgcagaaa aaaatgcctg atgcgacgct gcgcgtctta tactcccaca tatgccagat
     8701 tcagcaacgg atacggcttc cccaacttgc ccacttccat acgtgtcctc cttaccagaa
     8761 atttatcctt aaggtcgtca gctatcctgc aggcgatctc tcgatttcga tcaagacatt
     8821 cctttaatgg tcttttctgg acaccactag gggtcagaag tagttcatca aactttcttc
     8881 cctccctaat ctcattggtt accttgggct atcgaaactt aattaaccag tcaagtcagc
     8941 tacttggcga gatcgacttg tctgggtttc gactacgctc agaattgcgt cagtcaagtt
     9001 cgatctggtc cttgctattg cacccgttct ccgattacga gtttcattta aatcatgtga
     9061 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9121 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9181 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9241 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9301 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9361 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9421 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9481 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9541 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9601 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9661 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9721 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9781 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9841 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9901 atctcagcga tctgtctatt tcgttcatcc atagttgcat ttaaatttcc gaactctcca
     9961 aggccctcgt cggaaaatct tcaaaccttt cgtccgatcc atcttgcagg ctacctctcg
    10021 aacgaactat cgcaagtctc ttggccggcc ttgcgccttg gctattgctt ggcagcgcct
    10081 atcgccaggt attactccaa tcccgaatat ccgagatcgg gatcacccga gagaagttca
    10141 acctacatcc tcaatcccga tctatccgag atccgaggaa tatcgaaatc ggggcgcgcc
    10201 tg
//
