LOCUS       sequence-486425-       10191 bp    DNA     circular SYN 17-SEP-2025
DEFINITION  Dual luciferase (nano, firefly) with Zfp423 5' end fused to 
            NanoLuciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Concepcion D, Liang C, Kim D, Hamilton BA
  TITLE     Nonequivalence of Zfp423 premature termination codons in mice.
  JOURNAL   bioRxiv [Preprint]. 2025 Jun 2:2025.05.30.656936. doi: 
            10.1101/2025.05.30.656936.
  PUBMED    40501617
REFERENCE   2  (bases 1 to 10191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Jun 2:2025.05.30.656936. doi: 
            10.1101/2025.05.30.656936."
COMMENT     Sequence Label: pZNpF-∆11
FEATURES             Location/Qualifiers
     source          1..10191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1210
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1047..1066
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1214..1813
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1214..1233)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1710..1730
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    1840..1888
                     /note="synthetic polyadenylation signal"
     CDS             join(2154..2222,2223..3014)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2372..2391)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3027..3163
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3315..3386
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3391..3437)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3455..3503
                     /note="synthetic polyadenylation signal"
     promoter        3540..4718
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3771..4709
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4666..4686
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5250..5762
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     promoter        6005..6515
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6434..6452
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6612..8264
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    8314..8435
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8325..8344)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8379..8398
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      8550..8596
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      8626..8762
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    8807..8878
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(9104..9692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9184..9203)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcgtcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc aggagcgtcc tgatccttcc gcccggacgc tcaggacagc
      121 ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg acattttagg
      181 acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga
      241 aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg
      301 attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc
      361 gcggttcttg tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg
      421 gccggggctt tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc
      481 aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc
      541 agcaaaatgg cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag
      601 gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgagcccttc
      661 gctaatgcgg gaaagctctt attcgggtga gatgggctgg gcaccatctg gggaccctga
      721 cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta
      781 tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt
      841 gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgcg
      901 gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg
      961 acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt
     1021 tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt
     1081 ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat
     1141 atgtaatttt cagtgttaga cttgtaaatt gtccgctaaa ttctggccgt ttttggcttt
     1201 tttgttagac aacatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     1261 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     1321 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     1381 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     1441 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     1501 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gagggcctcc tggcgccgca
     1561 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     1621 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gccgccgagc gcgccggggt
     1681 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     1741 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     1801 gcccggtgcc taggctagta tgtaagccta gtcttagata ataaaatcgc tatccatcga
     1861 agatggatgt gtgttggttt tttgtgtgtg taacgctagg cgcgcctggt gtaccgagaa
     1921 cgatcctctc agtgcgagtc tcgacgatcc atatcgttgc ttggcagtca gccagtcgga
     1981 atccagcttg ggacccagga agtccaatcg tcagatattg tactcaagcc tggtcacggc
     2041 agcgtaccga tctgtttaaa cctagatatt gatagtctga tcggtcaacg tataatcgag
     2101 tcctagcttt tgcaaacatc tatcaagaga caggatcagc aggaggcttt cgcatgagta
     2161 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     2221 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcgcgagtgg
     2281 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     2341 gctttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2401 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2461 attcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2521 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg attggaggac
     2581 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2641 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2701 caatggcaac aaccttgcgt aaactattaa ctggcgaact acttactcta gcttcccggc
     2761 aacagttgat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2821 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2881 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2941 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     3001 ttaagcattg gtaaccgatt ctaggtgcat tggcgcagaa aaaaatgcct gatgcgacgc
     3061 tgcgcgtctt atactcccac atatgccaga ttcagcaacg gatacggctt ccccaacttg
     3121 cccacttcca tacgtgtcct ccttaccaga aatttatcct taagatcccg aatcgtttaa
     3181 actcgactct ggctctatcg aatctccgtc gtttcgagct tacgcgaaca gccgtggcgc
     3241 tcatttgctc gtcgggcatc gaatctcgtc agctatcgtc agcttacctt tttggcagcg
     3301 atcgcggctc ccgacatctt ggaccattag ctccacaggt atcttcttcc ctctagtggt
     3361 cataacagca gcttcagcta cctctcaatt caaaaaaccc ctcaagaccc gtttagaggc
     3421 cccaaggggt tatgctatca atcgttgcgt tacacacaca aaaaaccaac acacatccat
     3481 cttcgatgga tagcgatttt attatctaac tgctgatcga gtgtagccag atctcgtgag
     3541 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     3601 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     3661 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcac
     3721 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     3781 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     3841 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     3901 agagttcgtg gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gtggcctggc
     3961 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     4021 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     4081 aagatagtct tgtaaatgcg ggccaagatc agcacactgg tatttcggtt tttggggccg
     4141 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     4201 cgcggccacc gagaatcgga cgggggtagt ctcaagctgc ccggcctgct ctggtgcctg
     4261 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     4321 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagcaca aaatggagga
     4381 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     4441 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     4501 agttctccag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     4561 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     4621 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     4681 tggttcaaag tttttttctt ccatttcagg tgtcgtgaaa actaccccta aaagccaaaa
     4741 gatctttgtc gatcctacca tccactcgac acacccgcca gcggccgctg ccaagcttcc
     4801 gagctctcga attcagccga ccgcggacgg cgacggagcg cccggagccc cggacatgtc
     4861 caggcggaag caggcgaagc cgcgatcggt gaaagttgaa gagggggagg cctcggactt
     4921 ctcgctggcc tgggattcct ctgtggcagc agcaggaggc ctggaaggag agccagagtg
     4981 tgatcggaaa accagccgtg cgctggaaga caggaacagc gtgacaagtc aagaggagag
     5041 aaatgaggac gatgaagacg tggaagatga gtcaatttac acctgcgatc actgtcagca
     5101 ggacttcgag tctctggcag acctgacgga ccaccgggcc tggagatggt gatgacgacc
     5161 cacagctctc ctgggtggct tcatctccct ccagcaagga tgttgcgtca cctacgcaga
     5221 tgataggagg aggtggatcc ggaggcggag tcttcacact cgaagatttc gttggggact
     5281 ggcgacagac agccggctac aacctggacc aagtccttga acagggaggt gtgtccagtt
     5341 tgtttcagaa tctcggggtg tccgtaactc cgatccaaag gattgtcctg agcggtgaaa
     5401 atgggctgaa gatcgacatc catgtcatca tcccgtatga aggtctgagc ggcgaccaaa
     5461 tgggccagat cgaaaaaatt tttaaggtgg tgtaccctgt ggatgatcat cactttaagg
     5521 tgatcctgca ctatggcaca ctggtaatcg acggggttac gccgaacatg atcgactatt
     5581 tcggacggcc gtatgaaggc atcgccgtgt tcgacggcaa aaagatcact gtaacaggga
     5641 ccctgtggaa cggcaacaaa attatcgacg agcgcctgat caaccccgac ggctccctgc
     5701 tgttccgagt aaccatcaac ggagtgaccg gctggcggct gtgcgaacgc attctggcgt
     5761 aatattaaga aaccctggac cacccacccc agcaaggaca ctgagcaaga gaggccctat
     5821 cccaactcgg cccccaacac tgagcatctc cctcacaatt tccatcccag acccccataa
     5881 taacaggagg ggcccaggga gccctcccta ctctcttgaa taccatcaat aaagttcgct
     5941 gcacccacct ctccttggtt ttaatgcggt gttgggggag ggagggtgtg taaataaatc
     6001 tcgaggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
     6061 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
     6121 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
     6181 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
     6241 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
     6301 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
     6361 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
     6421 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
     6481 ccctcgttga ccgaatcacc gacctctctc cccagctgac cacttcggtc ctgagcgctt
     6541 gggagttagg ttgtttgccg gcgtagcggc cagcgcctga gcccgccctt gatcttcgct
     6601 gtggcgtcga catggaagat gccaaaaaca ttaagaaggg cccagcgcca ttctacccac
     6661 tcgaagacgg gaccgccggc gagcaactgc acaaagccat gaagcgctac gccctggtgc
     6721 ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac gccgagtact
     6781 tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat acaaaccatc
     6841 ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg ggtgccctgt
     6901 tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag ctgctgaaca
     6961 gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc
     7021 tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat agcaagaccg
     7081 actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca cccggcttca
     7141 acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc ctgatcatga
     7201 acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc accgcttgtg
     7261 tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc gacaccgcta
     7321 tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg ggctacttga
     7381 tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc ttgcgcagct
     7441 tgcaagacta taagattcaa tctgccctgc tggtgcccac actctttagc ttcttcgcta
     7501 agagcactct gattgataag tacgacctaa gcaacttgca cgagatcgcc agcggcgggg
     7561 cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta ccaggcatcc
     7621 gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc gaaggggacg
     7681 acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg gtggacttgg
     7741 acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt ggccccatga
     7801 tcatgagcgg ctacgttaac aacccagagg ctacaaacgc tctcatcgac aaggacggct
     7861 ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc atcgtggacc
     7921 ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa ctggagagca
     7981 tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc gacgacgatg
     8041 ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg accgagaagg
     8101 agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc ggtggtgttg
     8161 tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc aagatccgcg
     8221 agattctcat taaggccaag aagggcggca agatcgccgt gtaagctagc ttgactgact
     8281 gagatacagc gtaccttcag ctcacagaca tgataagata cattgatgag tttggacaaa
     8341 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     8401 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     8461 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     8521 gtggtattgg cccatctcta tcggtatcgt agcataaccc cttggggcct ctaaacgggt
     8581 cttgaggggt tttttgtgcc cctcgggccg gattgctatc taccggcatt ggcgcagaaa
     8641 aaaatgcctg atgcgacgct gcgcgtctta tactcccaca tatgccagat tcagcaacgg
     8701 atacggcttc cccaacttgc ccacttccat acgtgtcctc cttaccagaa atttatcctt
     8761 aaggtcgtca gctatcctgc aggcgatctc tcgatttcga tcaagacatt cctttaatgg
     8821 tcttttctgg acaccactag gggtcagaag tagttcatca aactttcttc cctccctaat
     8881 ctcattggtt accttgggct atcgaaactt aattaaccag tcaagtcagc tacttggcga
     8941 gatcgacttg tctgggtttc gactacgctc agaattgcgt cagtcaagtt cgatctggtc
     9001 cttgctattg cacccgttct ccgattacga gtttcattta aatcatgtga gcaaaaggcc
     9061 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9121 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9181 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9241 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9301 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9361 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9421 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9481 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9541 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9601 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9661 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9721 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9781 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9841 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9901 tctgtctatt tcgttcatcc atagttgcat ttaaatttcc gaactctcca aggccctcgt
     9961 cggaaaatct tcaaaccttt cgtccgatcc atcttgcagg ctacctctcg aacgaactat
    10021 cgcaagtctc ttggccggcc ttgcgccttg gctattgctt ggcagcgcct atcgccaggt
    10081 attactccaa tcccgaatat ccgagatcgg gatcacccga gagaagttca acctacatcc
    10141 tcaatcccga tctatccgag atccgaggaa tatcgaaatc ggggcgcgcc t
//
