LOCUS       sequence-486431-        9927 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Negative control, firefly luciferase mutated.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Concepcion D, Liang C, Kim D, Hamilton BA
  TITLE     Nonequivalence of Zfp423 premature termination codons in mice.
  JOURNAL   bioRxiv [Preprint]. 2025 Jun 2:2025.05.30.656936. doi: 
            10.1101/2025.05.30.656936.
  PUBMED    40501617
REFERENCE   2  (bases 1 to 9927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Jun 2:2025.05.30.656936. doi: 
            10.1101/2025.05.30.656936."
COMMENT     Sequence Label: pZNp-2
FEATURES             Location/Qualifiers
     source          1..9927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1046..1065
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1213..1812
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1213..1232)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1709..1729
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    1839..1887
                     /note="synthetic polyadenylation signal"
     CDS             join(2153..2221,2222..3013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2371..2390)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3026..3162
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3314..3385
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3390..3436)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3454..3502
                     /note="synthetic polyadenylation signal"
     promoter        3539..4717
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3770..4708
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4665..4685
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5260..5772
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     primer_bind     6168..6186
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     polyA_signal    8049..8170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8060..8079)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8114..8133
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      8285..8331
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      8361..8497
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    8542..8613
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(8839..9427)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8919..8938)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(9927,1..1209)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcgtcacgg cgagcgctgc
       61 cacgtcagac gaagggcgca ggagcgtcct gatccttccg cccggacgct caggacagcg
      121 gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga
      181 cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa
      241 aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga
      301 ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg
      361 cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg
      421 ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca
      481 agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca
      541 gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg
      601 tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gagcccttcg
      661 ctaatgcggg aaagctctta ttcgggtgag atgggctggg caccatctgg ggaccctgac
      721 gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat
      781 ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg
      841 acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg
      901 taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga
      961 caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt
     1021 ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg
     1081 gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata
     1141 tgtaattttc agtgttagac ttgtaaattg tccgctaaat tctggccgtt tttggctttt
     1201 ttgttagaca acatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     1261 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     1321 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     1381 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     1441 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     1501 gagttgagcg gttcccggct ggccgcgcag caacagatgg agggcctcct ggcgccgcac
     1561 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     1621 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg ccgccgagcg cgccggggtg
     1681 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     1741 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     1801 cccggtgcct aggctagtat gtaagcctag tcttagataa taaaatcgct atccatcgaa
     1861 gatggatgtg tgttggtttt ttgtgtgtgt aacgctaggc gcgcctggtg taccgagaac
     1921 gatcctctca gtgcgagtct cgacgatcca tatcgttgct tggcagtcag ccagtcggaa
     1981 tccagcttgg gacccaggaa gtccaatcgt cagatattgt actcaagcct ggtcacggca
     2041 gcgtaccgat ctgtttaaac ctagatattg atagtctgat cggtcaacgt ataatcgagt
     2101 cctagctttt gcaaacatct atcaagagac aggatcagca ggaggctttc gcatgagtat
     2161 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2221 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cgcgagtggg
     2281 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2341 ctttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2401 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2461 ttcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2521 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga ttggaggacc
     2581 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2641 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2701 aatggcaaca accttgcgta aactattaac tggcgaacta cttactctag cttcccggca
     2761 acagttgata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2821 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2881 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2941 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3001 taagcattgg taaccgattc taggtgcatt ggcgcagaaa aaaatgcctg atgcgacgct
     3061 gcgcgtctta tactcccaca tatgccagat tcagcaacgg atacggcttc cccaacttgc
     3121 ccacttccat acgtgtcctc cttaccagaa atttatcctt aagatcccga atcgtttaaa
     3181 ctcgactctg gctctatcga atctccgtcg tttcgagctt acgcgaacag ccgtggcgct
     3241 catttgctcg tcgggcatcg aatctcgtca gctatcgtca gcttaccttt ttggcagcga
     3301 tcgcggctcc cgacatcttg gaccattagc tccacaggta tcttcttccc tctagtggtc
     3361 ataacagcag cttcagctac ctctcaattc aaaaaacccc tcaagacccg tttagaggcc
     3421 ccaaggggtt atgctatcaa tcgttgcgtt acacacacaa aaaaccaaca cacatccatc
     3481 ttcgatggat agcgatttta ttatctaact gctgatcgag tgtagccaga tctcgtgagg
     3541 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     3601 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     3661 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcact
     3721 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
     3781 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
     3841 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
     3901 gagttcgtgg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg tggcctggcc
     3961 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
     4021 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
     4081 agatagtctt gtaaatgcgg gccaagatca gcacactggt atttcggttt ttggggccgc
     4141 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     4201 gcggccaccg agaatcggac gggggtagtc tcaagctgcc cggcctgctc tggtgcctgg
     4261 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     4321 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagcacaa aatggaggac
     4381 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     4441 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     4501 gttctccagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     4561 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     4621 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     4681 ggttcaaagt ttttttcttc catttcaggt gtcgtgaaaa ctacccctaa aagccaaaag
     4741 atctttgtcg atcctaccat ccactcgaca cacccgccag cggccgctgc caagcttccg
     4801 agctctcgaa ttcagccgac cgcggacggc gacggagcgc ccggagcccc ggacatgtcc
     4861 aggcggaagc aggcgaagcc gcgatcggtg aaagttgaag agggggaggc ctcggacttc
     4921 tcgctggcct gggattcctc tgtggcagca gcaggaggcc tggaaggaga gccagagtgt
     4981 gatcggaaaa ccagccgtgc gctggaagac aggaacagcg tgacaagtca agaggagaga
     5041 aatgaggacg atgaagacgt ggaagatgag tcaatttaca cctgcgatca ctgtcagcag
     5101 gacttcgagt ctctggcaga cctgacggac caccgggccc accgctgtcc tggagatggt
     5161 gatgacgacc cacagctctc ctgggtggct tcatctccct ccagcaagga tgttgcgtca
     5221 cctacgcaga tgataggagg aggtggatcc ggaggcggag tcttcacact cgaagatttc
     5281 gttggggact ggcgacagac agccggctac aacctggacc aagtccttga acagggaggt
     5341 gtgtccagtt tgtttcagaa tctcggggtg tccgtaactc cgatccaaag gattgtcctg
     5401 agcggtgaaa atgggctgaa gatcgacatc catgtcatca tcccgtatga aggtctgagc
     5461 ggcgaccaaa tgggccagat cgaaaaaatt tttaaggtgg tgtaccctgt ggatgatcat
     5521 cactttaagg tgatcctgca ctatggcaca ctggtaatcg acggggttac gccgaacatg
     5581 atcgactatt tcggacggcc gtatgaaggc atcgccgtgt tcgacggcaa aaagatcact
     5641 gtaacaggga ccctgtggaa cggcaacaaa attatcgacg agcgcctgat caaccccgac
     5701 ggctccctgc tgttccgagt aaccatcaac ggagtgaccg gctggcggct gtgcgaacgc
     5761 attctggcgt aatattaaga aaccctggac cacccacccc agcaaggaca ctgagcaaga
     5821 gaggccctat cccaactcgg cccccaacac tgagcatctc cctcacaatt tccatcccag
     5881 acccccataa taacaggagg ggcctaggga gccctcccta ctctcttgaa taccatcaat
     5941 aaagttcgct gcacccacct ctccttggtt ttaatgcggt gttgggggag ggagggtgtg
     6001 taaataaatc tcgaggggtt ggggttgcgt cttttccaag gcagccctgg gtttgcgcag
     6061 ggacgcggct gctctgggcg tggttccggg aaacgcagcg gccgggaagg ggcggtgcgg
     6121 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc
     6181 tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc
     6241 tctccccagc tgaccacttc ggtcctgagc gcttgggagt taggttgttt gccggcgtag
     6301 cggccagcgc ctgagcccgc ccttgatctt cgctgtggcg tcgacatgga agatgccaaa
     6361 aacattaaga agggcccagc gccattctac ccactcgaag acgggaccgc cggcgagcaa
     6421 ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt taccgacgca
     6481 catatcgagg tggacattac ctacgccgag tacttcgaga tgagcgttcg gctggcagaa
     6541 gctatgaagc gctatgggct gaatacaaac catcggatcg tggtgtgcag cgagaatagc
     6601 ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct
     6661 aacgacatct acaacgagcg cgagctgctg aacagcatgg gcatcagcca gcccaccgtc
     6721 gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa gctaccgatc
     6781 atacaaaaga tcatcatcat ggatagcaag accgactacc agggcttcca aagcatgtac
     6841 accttcgtga cttcccattt gccacccggc ttcaacgagt acgacttcgt gcccgagagc
     6901 ttcgaccggg acaaaaccat cgccctgatc atgaacagta gtggcagtac cggattgccc
     6961 aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc ccgcgacccc
     7021 atcttcggca accagatcat ccccgacacc gctatcctca gcgtggtgcc atttcaccac
     7081 ggcttcggca tgttcaccac gctgggctac ttgatctgcg gctttcgggt cgtgctcatg
     7141 taccgcttcg aggaggagct attcttgcgc agcttgcaag actataagat tcaatctgcc
     7201 ctgctggtgc ccacactctt tagcttcttc gctaagagca ctctgattga taagtacgac
     7261 ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag
     7321 gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct gacagaaaca
     7381 accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt aggcaaggtg
     7441 gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact gggtgtgaac
     7501 cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt taacaaccca
     7561 gaggctacaa acgctctcat cgacaaggac ggctggctgc acagcggccg acatcgccta
     7621 ctgggacgag gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa
     7681 gggctaccag gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt
     7741 cgacgccggg gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt
     7801 cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca
     7861 ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg
     7921 actgaccggc aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg
     7981 cggcaagatc gccgtgtaag ctagcttgac tgactgagat acagcgtacc ttcagctcac
     8041 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     8101 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     8161 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     8221 ggaggttttt taaagcaagt aaaacctcta caaatgtggt attggcccat ctctatcggt
     8281 atcgtagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gtgcccctcg
     8341 ggccggattg ctatctaccg gcattggcgc agaaaaaaat gcctgatgcg acgctgcgcg
     8401 tcttatactc ccacatatgc cagattcagc aacggatacg gcttccccaa cttgcccact
     8461 tccatacgtg tcctccttac cagaaattta tccttaaggt cgtcagctat cctgcaggcg
     8521 atctctcgat ttcgatcaag acattccttt aatggtcttt tctggacacc actaggggtc
     8581 agaagtagtt catcaaactt tcttccctcc ctaatctcat tggttacctt gggctatcga
     8641 aacttaatta accagtcaag tcagctactt ggcgagatcg acttgtctgg gtttcgacta
     8701 cgctcagaat tgcgtcagtc aagttcgatc tggtccttgc tattgcaccc gttctccgat
     8761 tacgagtttc atttaaatca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8821 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8881 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8941 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9001 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9061 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9121 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9181 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9241 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9301 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9361 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9421 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9481 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9541 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9601 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9661 tgcatttaaa tttccgaact ctccaaggcc ctcgtcggaa aatcttcaaa cctttcgtcc
     9721 gatccatctt gcaggctacc tctcgaacga actatcgcaa gtctcttggc cggccttgcg
     9781 ccttggctat tgcttggcag cgcctatcgc caggtattac tccaatcccg aatatccgag
     9841 atcgggatca cccgagagaa gttcaaccta catcctcaat cccgatctat ccgagatccg
     9901 aggaatatcg aaatcggggc gcgcctg
//
