LOCUS       sequence-486433-       10195 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Dual luciferase (nano, firefly) with Zfp423 5' end fused to 
            NanoLuciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Concepcion D, Liang C, Kim D, Hamilton BA
  TITLE     Nonequivalence of Zfp423 premature termination codons in mice.
  JOURNAL   bioRxiv [Preprint]. 2025 Jun 2:2025.05.30.656936. doi: 
            10.1101/2025.05.30.656936.
  PUBMED    40501617
REFERENCE   2  (bases 1 to 10195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Jun 2:2025.05.30.656936. doi: 
            10.1101/2025.05.30.656936."
COMMENT     Sequence Label: pZNpF-∆7cg
FEATURES             Location/Qualifiers
     source          1..10195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1046..1065
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1213..1812
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1213..1232)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1709..1729
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    1839..1887
                     /note="synthetic polyadenylation signal"
     CDS             join(2153..2221,2222..3013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2371..2390)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3026..3162
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3314..3385
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3390..3436)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3454..3502
                     /note="synthetic polyadenylation signal"
     promoter        3539..4717
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3770..4708
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4665..4685
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5253..5765
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     promoter        6008..6518
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6437..6455
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6615..8267
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    8317..8438
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8328..8347)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8382..8401
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      8553..8599
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      8629..8765
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    8810..8881
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(9107..9695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9187..9206)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(10195,1..1209)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcgtcacgg cgagcgctgc
       61 cacgtcagac gaagggcgca ggagcgtcct gatccttccg cccggacgct caggacagcg
      121 gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga
      181 cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa
      241 aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga
      301 ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg
      361 cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg
      421 ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca
      481 agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca
      541 gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg
      601 tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gagcccttcg
      661 ctaatgcggg aaagctctta ttcgggtgag atgggctggg caccatctgg ggaccctgac
      721 gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat
      781 ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg
      841 acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg
      901 taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga
      961 caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt
     1021 ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg
     1081 gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata
     1141 tgtaattttc agtgttagac ttgtaaattg tccgctaaat tctggccgtt tttggctttt
     1201 ttgttagaca acatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     1261 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     1321 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     1381 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     1441 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     1501 gagttgagcg gttcccggct ggccgcgcag caacagatgg agggcctcct ggcgccgcac
     1561 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     1621 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg ccgccgagcg cgccggggtg
     1681 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     1741 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     1801 cccggtgcct aggctagtat gtaagcctag tcttagataa taaaatcgct atccatcgaa
     1861 gatggatgtg tgttggtttt ttgtgtgtgt aacgctaggc gcgcctggtg taccgagaac
     1921 gatcctctca gtgcgagtct cgacgatcca tatcgttgct tggcagtcag ccagtcggaa
     1981 tccagcttgg gacccaggaa gtccaatcgt cagatattgt actcaagcct ggtcacggca
     2041 gcgtaccgat ctgtttaaac ctagatattg atagtctgat cggtcaacgt ataatcgagt
     2101 cctagctttt gcaaacatct atcaagagac aggatcagca ggaggctttc gcatgagtat
     2161 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2221 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cgcgagtggg
     2281 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2341 ctttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2401 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2461 ttcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2521 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga ttggaggacc
     2581 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2641 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2701 aatggcaaca accttgcgta aactattaac tggcgaacta cttactctag cttcccggca
     2761 acagttgata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2821 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2881 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2941 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3001 taagcattgg taaccgattc taggtgcatt ggcgcagaaa aaaatgcctg atgcgacgct
     3061 gcgcgtctta tactcccaca tatgccagat tcagcaacgg atacggcttc cccaacttgc
     3121 ccacttccat acgtgtcctc cttaccagaa atttatcctt aagatcccga atcgtttaaa
     3181 ctcgactctg gctctatcga atctccgtcg tttcgagctt acgcgaacag ccgtggcgct
     3241 catttgctcg tcgggcatcg aatctcgtca gctatcgtca gcttaccttt ttggcagcga
     3301 tcgcggctcc cgacatcttg gaccattagc tccacaggta tcttcttccc tctagtggtc
     3361 ataacagcag cttcagctac ctctcaattc aaaaaacccc tcaagacccg tttagaggcc
     3421 ccaaggggtt atgctatcaa tcgttgcgtt acacacacaa aaaaccaaca cacatccatc
     3481 ttcgatggat agcgatttta ttatctaact gctgatcgag tgtagccaga tctcgtgagg
     3541 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     3601 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     3661 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcact
     3721 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
     3781 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
     3841 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
     3901 gagttcgtgg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg tggcctggcc
     3961 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
     4021 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
     4081 agatagtctt gtaaatgcgg gccaagatca gcacactggt atttcggttt ttggggccgc
     4141 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     4201 gcggccaccg agaatcggac gggggtagtc tcaagctgcc cggcctgctc tggtgcctgg
     4261 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     4321 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagcacaa aatggaggac
     4381 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     4441 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     4501 gttctccagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     4561 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     4621 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     4681 ggttcaaagt ttttttcttc catttcaggt gtcgtgaaaa ctacccctaa aagccaaaag
     4741 atctttgtcg atcctaccat ccactcgaca cacccgccag cggccgctgc caagcttccg
     4801 agctctcgaa ttcagccgac cgcggacggc gacggagcgc ccggagcccc ggacatgtcc
     4861 aggcggaagc aggcgaagcc gcgatcggtg aaagttgaag agggggaggc ctcggacttc
     4921 tcgctggcct gggattcctc tgtggcagca gcaggaggcc tggaaggaga gccagagtgt
     4981 gatcggaaaa ccagccgtgc gctggaagac aggaacagcg tgacaagtca agaggagaga
     5041 aatgaggacg atgaagacgt ggaagatgag tcaatttaca cctgcgacca ctgtcagcag
     5101 gacttcgagt ctctggcaga cctgacggac caccgggctg tcctggagag ggtgatgacg
     5161 acccacagct ctcctgggtg gcttcatctc cctccagcaa ggatgttgcg tcacctacgc
     5221 agatgatagg aggaggtgga tccggaggcg gagtcttcac actcgaagat ttcgttgggg
     5281 actggcgaca gacagccggc tacaacctgg accaagtcct tgaacaggga ggtgtgtcca
     5341 gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca aaggattgtc ctgagcggtg
     5401 aaaatgggct gaagatcgac atccatgtca tcatcccgta tgaaggtctg agcggcgacc
     5461 aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc tgtggatgat catcacttta
     5521 aggtgatcct gcactatggc acactggtaa tcgacggggt tacgccgaac atgatcgact
     5581 atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg caaaaagatc actgtaacag
     5641 ggaccctgtg gaacggcaac aaaattatcg acgagcgcct gatcaacccc gacggctccc
     5701 tgctgttccg agtaaccatc aacggagtga ccggctggcg gctgtgcgaa cgcattctgg
     5761 cgtaatatta agaaaccctg gaccacccac cccagcaagg acactgagca agagaggccc
     5821 tatcccaact cggcccccaa cactgagcat ctccctcaca atttccatcc cagaccccca
     5881 taataacagg aggggcccag ggagccctcc ctactctctt gaataccatc aataaagttc
     5941 gctgcaccca cctctccttg gttttaatgc ggtgttgggg gagggagggt gtgtaaataa
     6001 atctcgaggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg
     6061 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct
     6121 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga
     6181 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt
     6241 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa
     6301 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg
     6361 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc
     6421 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg
     6481 gctccctcgt tgaccgaatc accgacctct ctccccagct gaccacttcg gtcctgagcg
     6541 cttgggagtt aggttgtttg ccggcgtagc ggccagcgcc tgagcccgcc cttgatcttc
     6601 gctgtggcgt cgacatggaa gatgccaaaa acattaagaa gggcccagcg ccattctacc
     6661 cactcgaaga cgggaccgcc ggcgagcaac tgcacaaagc catgaagcgc tacgccctgg
     6721 tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc tacgccgagt
     6781 acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg aatacaaacc
     6841 atcggatcgt ggtgtgcagc gagaatagct tgcagttctt catgcccgtg ttgggtgccc
     6901 tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacgagcgc gagctgctga
     6961 acagcatggg catcagccag cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga
     7021 tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat catcatcatg gatagcaaga
     7081 ccgactacca gggcttccaa agcatgtaca ccttcgtgac ttcccatttg ccacccggct
     7141 tcaacgagta cgacttcgtg cccgagagct tcgaccggga caaaaccatc gccctgatca
     7201 tgaacagtag tggcagtacc ggattgccca agggcgtagc cctaccgcac cgcaccgctt
     7261 gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc cccgacaccg
     7321 ctatcctcag cgtggtgcca tttcaccacg gcttcggcat gttcaccacg ctgggctact
     7381 tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga ggaggagcta ttcttgcgca
     7441 gcttgcaaga ctataagatt caatctgccc tgctggtgcc cacactcttt agcttcttcg
     7501 ctaagagcac tctgattgat aagtacgacc taagcaactt gcacgagatc gccagcggcg
     7561 gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa acgcttccac ctaccaggca
     7621 tccgccaggg ctacggcctg acagaaacaa ccagcgccat tctgatcacc cccgaagggg
     7681 acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag gtggtggact
     7741 tggacaccgg taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca
     7801 tgatcatgag cggctacgtt aacaacccag aggctacaaa cgctctcatc gacaaggacg
     7861 gctggctgca cagcggcgac atcgcctact gggacgagga cgagcacttc ttcatcgtgg
     7921 accggctgaa gagcctgatc aaatacaagg gctaccaggt agccccagcc gaactggaga
     7981 gcatcctgct gcaacacccc aacatcttcg acgccggggt cgccggcctg cccgacgacg
     8041 atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga
     8101 aggagatcgt ggactatgtg gccagccagg ttacaaccgc caagaagctg cgcggtggtg
     8161 ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc
     8221 gcgagattct cattaaggcc aagaagggcg gcaagatcgc cgtgtaagct agcttgactg
     8281 actgagatac agcgtacctt cagctcacag acatgataag atacattgat gagtttggac
     8341 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     8401 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     8461 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     8521 aatgtggtat tggcccatct ctatcggtat cgtagcataa ccccttgggg cctctaaacg
     8581 ggtcttgagg ggttttttgt gcccctcggg ccggattgct atctaccggc attggcgcag
     8641 aaaaaaatgc ctgatgcgac gctgcgcgtc ttatactccc acatatgcca gattcagcaa
     8701 cggatacggc ttccccaact tgcccacttc catacgtgtc ctccttacca gaaatttatc
     8761 cttaaggtcg tcagctatcc tgcaggcgat ctctcgattt cgatcaagac attcctttaa
     8821 tggtcttttc tggacaccac taggggtcag aagtagttca tcaaactttc ttccctccct
     8881 aatctcattg gttaccttgg gctatcgaaa cttaattaac cagtcaagtc agctacttgg
     8941 cgagatcgac ttgtctgggt ttcgactacg ctcagaattg cgtcagtcaa gttcgatctg
     9001 gtccttgcta ttgcacccgt tctccgatta cgagtttcat ttaaatcatg tgagcaaaag
     9061 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9121 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9181 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9241 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9301 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9361 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9421 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9481 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9541 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9601 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9661 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9721 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9781 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9841 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9901 cgatctgtct atttcgttca tccatagttg catttaaatt tccgaactct ccaaggccct
     9961 cgtcggaaaa tcttcaaacc tttcgtccga tccatcttgc aggctacctc tcgaacgaac
    10021 tatcgcaagt ctcttggccg gccttgcgcc ttggctattg cttggcagcg cctatcgcca
    10081 ggtattactc caatcccgaa tatccgagat cgggatcacc cgagagaagt tcaacctaca
    10141 tcctcaatcc cgatctatcc gagatccgag gaatatcgaa atcggggcgc gcctg
//
