LOCUS       sequence-486434-       10191 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Dual luciferase (nano, firefly) with Zfp423 5' end fused to 
            NanoLuciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Concepcion D, Liang C, Kim D, Hamilton BA
  TITLE     Nonequivalence of Zfp423 premature termination codons in mice.
  JOURNAL   bioRxiv [Preprint]. 2025 Jun 2:2025.05.30.656936. doi: 
            10.1101/2025.05.30.656936.
  PUBMED    40501617
REFERENCE   2  (bases 1 to 10191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Jun 2:2025.05.30.656936. doi: 
            10.1101/2025.05.30.656936."
COMMENT     Sequence Label: pZNpF-∆11cag
FEATURES             Location/Qualifiers
     source          1..10191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1046..1065
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1213..1812
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1213..1232)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1709..1729
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    1839..1887
                     /note="synthetic polyadenylation signal"
     CDS             join(2153..2221,2222..3013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2371..2390)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3026..3162
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3314..3385
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3390..3436)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3454..3502
                     /note="synthetic polyadenylation signal"
     promoter        3539..4717
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3770..4708
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4665..4685
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5249..5761
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     promoter        6004..6514
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6433..6451
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6611..8263
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    8313..8434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8324..8343)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8378..8397
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      8549..8595
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      8625..8761
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    8806..8877
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(9103..9691)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9183..9202)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(10191,1..1209)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcgtcacgg cgagcgctgc
       61 cacgtcagac gaagggcgca ggagcgtcct gatccttccg cccggacgct caggacagcg
      121 gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga
      181 cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa
      241 aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga
      301 ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg
      361 cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg
      421 ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca
      481 agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca
      541 gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg
      601 tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gagcccttcg
      661 ctaatgcggg aaagctctta ttcgggtgag atgggctggg caccatctgg ggaccctgac
      721 gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat
      781 ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg
      841 acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg
      901 taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga
      961 caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt
     1021 ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg
     1081 gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata
     1141 tgtaattttc agtgttagac ttgtaaattg tccgctaaat tctggccgtt tttggctttt
     1201 ttgttagaca acatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     1261 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     1321 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     1381 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     1441 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     1501 gagttgagcg gttcccggct ggccgcgcag caacagatgg agggcctcct ggcgccgcac
     1561 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     1621 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg ccgccgagcg cgccggggtg
     1681 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     1741 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     1801 cccggtgcct aggctagtat gtaagcctag tcttagataa taaaatcgct atccatcgaa
     1861 gatggatgtg tgttggtttt ttgtgtgtgt aacgctaggc gcgcctggtg taccgagaac
     1921 gatcctctca gtgcgagtct cgacgatcca tatcgttgct tggcagtcag ccagtcggaa
     1981 tccagcttgg gacccaggaa gtccaatcgt cagatattgt actcaagcct ggtcacggca
     2041 gcgtaccgat ctgtttaaac ctagatattg atagtctgat cggtcaacgt ataatcgagt
     2101 cctagctttt gcaaacatct atcaagagac aggatcagca ggaggctttc gcatgagtat
     2161 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2221 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cgcgagtggg
     2281 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2341 ctttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2401 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2461 ttcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2521 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga ttggaggacc
     2581 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2641 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2701 aatggcaaca accttgcgta aactattaac tggcgaacta cttactctag cttcccggca
     2761 acagttgata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2821 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2881 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2941 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3001 taagcattgg taaccgattc taggtgcatt ggcgcagaaa aaaatgcctg atgcgacgct
     3061 gcgcgtctta tactcccaca tatgccagat tcagcaacgg atacggcttc cccaacttgc
     3121 ccacttccat acgtgtcctc cttaccagaa atttatcctt aagatcccga atcgtttaaa
     3181 ctcgactctg gctctatcga atctccgtcg tttcgagctt acgcgaacag ccgtggcgct
     3241 catttgctcg tcgggcatcg aatctcgtca gctatcgtca gcttaccttt ttggcagcga
     3301 tcgcggctcc cgacatcttg gaccattagc tccacaggta tcttcttccc tctagtggtc
     3361 ataacagcag cttcagctac ctctcaattc aaaaaacccc tcaagacccg tttagaggcc
     3421 ccaaggggtt atgctatcaa tcgttgcgtt acacacacaa aaaaccaaca cacatccatc
     3481 ttcgatggat agcgatttta ttatctaact gctgatcgag tgtagccaga tctcgtgagg
     3541 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     3601 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     3661 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcact
     3721 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
     3781 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
     3841 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
     3901 gagttcgtgg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg tggcctggcc
     3961 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
     4021 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
     4081 agatagtctt gtaaatgcgg gccaagatca gcacactggt atttcggttt ttggggccgc
     4141 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     4201 gcggccaccg agaatcggac gggggtagtc tcaagctgcc cggcctgctc tggtgcctgg
     4261 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     4321 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagcacaa aatggaggac
     4381 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     4441 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     4501 gttctccagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     4561 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     4621 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     4681 ggttcaaagt ttttttcttc catttcaggt gtcgtgaaaa ctacccctaa aagccaaaag
     4741 atctttgtcg atcctaccat ccactcgaca cacccgccag cggccgctgc caagcttccg
     4801 agctctcgaa ttcagccgac cgcggacggc gacggagcgc ccggagcccc ggacatgtcc
     4861 aggcggaagc aggcgaagcc gcgatcggtg aaagttgaag agggggaggc ctcggacttc
     4921 tcgctggcct gggattcctc tgtggcagca gcaggaggcc tggaaggaga gccagagtgt
     4981 gatcggaaaa ccagccgtgc gctggaagac aggaacagcg tgacaagtca agaggagaga
     5041 aatgaggacg atgaagacgt ggaagatgag tcaatttaca cctgcgacca atgtcagcag
     5101 gacttcgagt ctctggcaga cctgacggac caccgggcct ggagagggtg atgacgaccc
     5161 acagctctcc tgggtggctt catctccctc cagcaaggat gttgcgtcac ctacgcagat
     5221 gataggagga ggtggatccg gaggcggagt cttcacactc gaagatttcg ttggggactg
     5281 gcgacagaca gccggctaca acctggacca agtccttgaa cagggaggtg tgtccagttt
     5341 gtttcagaat ctcggggtgt ccgtaactcc gatccaaagg attgtcctga gcggtgaaaa
     5401 tgggctgaag atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg gcgaccaaat
     5461 gggccagatc gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc actttaaggt
     5521 gatcctgcac tatggcacac tggtaatcga cggggttacg ccgaacatga tcgactattt
     5581 cggacggccg tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg taacagggac
     5641 cctgtggaac ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg gctccctgct
     5701 gttccgagta accatcaacg gagtgaccgg ctggcggctg tgcgaacgca ttctggcgta
     5761 atattaagaa accctggacc acccacccca gcaaggacac tgagcaagag aggccctatc
     5821 ccaactcggc ccccaacact gagcatctcc ctcacaattt ccatcccaga cccccataat
     5881 aacaggaggg gcccagggag ccctccctac tctcttgaat accatcaata aagttcgctg
     5941 cacccacctc tccttggttt taatgcggtg ttgggggagg gagggtgtgt aaataaatct
     6001 cgaggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg
     6061 ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac
     6121 gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct
     6181 tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca
     6241 aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc
     6301 agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag
     6361 cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt
     6421 cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc
     6481 cctcgttgac cgaatcaccg acctctctcc ccagctgacc acttcggtcc tgagcgcttg
     6541 ggagttaggt tgtttgccgg cgtagcggcc agcgcctgag cccgcccttg atcttcgctg
     6601 tggcgtcgac atggaagatg ccaaaaacat taagaagggc ccagcgccat tctacccact
     6661 cgaagacggg accgccggcg agcaactgca caaagccatg aagcgctacg ccctggtgcc
     6721 cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg ccgagtactt
     6781 cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata caaaccatcg
     6841 gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg gtgccctgtt
     6901 catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc tgctgaacag
     6961 catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc aaaagatcct
     7021 caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata gcaagaccga
     7081 ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac ccggcttcaa
     7141 cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc tgatcatgaa
     7201 cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca ccgcttgtgt
     7261 ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg acaccgctat
     7321 cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg gctacttgat
     7381 ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct tgcgcagctt
     7441 gcaagactat aagattcaat ctgccctgct ggtgcccaca ctctttagct tcttcgctaa
     7501 gagcactctg attgataagt acgacctaag caacttgcac gagatcgcca gcggcggggc
     7561 gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac caggcatccg
     7621 ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg aaggggacga
     7681 caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg tggacttgga
     7741 caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg gccccatgat
     7801 catgagcggc tacgttaaca acccagaggc tacaaacgct ctcatcgaca aggacggctg
     7861 gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca tcgtggaccg
     7921 gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac tggagagcat
     7981 cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg acgacgatgc
     8041 cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga ccgagaagga
     8101 gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg gtggtgttgt
     8161 gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca agatccgcga
     8221 gattctcatt aaggccaaga agggcggcaa gatcgccgtg taagctagct tgactgactg
     8281 agatacagcg taccttcagc tcacagacat gataagatac attgatgagt ttggacaaac
     8341 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     8401 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     8461 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     8521 tggtattggc ccatctctat cggtatcgta gcataacccc ttggggcctc taaacgggtc
     8581 ttgaggggtt ttttgtgccc ctcgggccgg attgctatct accggcattg gcgcagaaaa
     8641 aaatgcctga tgcgacgctg cgcgtcttat actcccacat atgccagatt cagcaacgga
     8701 tacggcttcc ccaacttgcc cacttccata cgtgtcctcc ttaccagaaa tttatcctta
     8761 aggtcgtcag ctatcctgca ggcgatctct cgatttcgat caagacattc ctttaatggt
     8821 cttttctgga caccactagg ggtcagaagt agttcatcaa actttcttcc ctccctaatc
     8881 tcattggtta ccttgggcta tcgaaactta attaaccagt caagtcagct acttggcgag
     8941 atcgacttgt ctgggtttcg actacgctca gaattgcgtc agtcaagttc gatctggtcc
     9001 ttgctattgc acccgttctc cgattacgag tttcatttaa atcatgtgag caaaaggcca
     9061 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9121 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9181 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9241 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9301 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9361 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9421 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9481 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9541 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9601 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9661 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9721 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9781 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9841 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9901 ctgtctattt cgttcatcca tagttgcatt taaatttccg aactctccaa ggccctcgtc
     9961 ggaaaatctt caaacctttc gtccgatcca tcttgcaggc tacctctcga acgaactatc
    10021 gcaagtctct tggccggcct tgcgccttgg ctattgcttg gcagcgccta tcgccaggta
    10081 ttactccaat cccgaatatc cgagatcggg atcacccgag agaagttcaa cctacatcct
    10141 caatcccgat ctatccgaga tccgaggaat atcgaaatcg gggcgcgcct g
//
