LOCUS       sequence-486437-        8920 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  expression vector or PiggyBac-insertable cargo for expressing 
            CITED2_K>A-HaloTag-ERD-SOX2DBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Fan V, Staller MV
  TITLE     Short activation domains control chromatin association of 
            transcription factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pVF202
FEATURES             Location/Qualifiers
     source          1..8920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     481..500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(719..823)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(849..1304)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(986..1007)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1198..1217
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1431..1453
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1445..1462
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1446..1462
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1592..1654
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2073..2572
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2108..2131)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2505..2524
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2594..3193
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2594..2613)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3090..3110
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(3220..3237)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3226..3450
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      3887..3896
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4028..4918
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             6038..6079
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6146..6166)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    6208..6329
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6245..6264)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6299..6318
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7092..7126
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7617..7633)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7617..7633)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7630..7652)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7641..7657
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7665..7671,7672..7689,7690..7695))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7710..7731
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7848..7865)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8019..8607)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8099..8118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8778..8920,1..649,650..718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
       61 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
      121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
      181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
      241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
      301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
      361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
      421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
      481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
      541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
      601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
      661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
      721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
      781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
      841 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
      901 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      961 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     1021 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     1081 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     1141 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     1201 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     1261 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     1321 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     1381 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     1441 acgttgtaaa acgacggcca gtgagcgcgc ctcgttcatt cacgtttttg aacccgtgga
     1501 ggacgggcag actcgcggtg caaatgtgtt ttacagcgtg atggagcaga tgaagatgct
     1561 cgacacgctg cagaacacgc agctagatta accctagaaa gataatcata ttgtgacgta
     1621 cgttaaagat aatcatgtgt aaaattgacg catgtgtttt atcggtctgt atatcgaggt
     1681 ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca tactaataat
     1741 aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaacaa aaactcaaaa
     1801 tttcttctat aaagtaacaa aacttttatg agggacagcc cccccccaaa gcccccaggg
     1861 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     1921 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     1981 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     2041 cctgggggga tacggggaaa aggcgaacgc gtgggtaggg gaggcgcttt tcccaaggca
     2101 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     2161 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     2221 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     2281 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     2341 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     2401 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     2461 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     2521 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     2581 cccaagcttc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     2641 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     2701 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     2761 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     2821 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     2881 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     2941 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     3001 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     3061 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     3121 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     3181 gcccggtgcc tgagcggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     3241 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     3301 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     3361 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     3421 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaacgcgat atgtacgggc
     3481 cagatatacg cgtcgcagaa caaacgccca gaaccgaagg ccggagccca gacgccgcag
     3541 cagcccagcc tgcgttgtaa cccggtcccc atagcaacca actaccgcag actactctcg
     3601 caagcgctgt tctgcttgtg acccggaccg gcttctcttc cgcttccggt cccacaatcc
     3661 tctgtcggcc tagaagagct ttgcattgtg ggagctcctt cctttctcgc tccccggcca
     3721 tcttggcggc tggtgttggt gagtgagctc tgcggggtaa acgattaggc ggctcgggga
     3781 gctccgctag ctggtgtttg acgctctgga ttataatcct ctcttctgtc ctctgtgtat
     3841 gctaggttgg gggttgtccc gcacctagag ctagcgccca gcttaagcca ccatggacac
     3901 cgacttcatc gacgaggagg tgctgatgag cctggtcatc gagatgggcc tggacagaat
     3961 cgcagaactg ccagagctgt ggctgggaca gaacgagttc gacttcatga ccgacggatc
     4021 cggcagcatg gccgagatcg gaaccggctt ccctttcgac cctcactacg tggaggtgct
     4081 gggagaacgg atgcactacg tggacgtggg acctagagac ggaacaccag tgctgttcct
     4141 gcacggaaac cccacaagca gctacgtctg gcggaacatc atcccccacg tggcccctac
     4201 acacagatgt atcgccccag acctgatcgg catgggaaag agcgacaagc ccgacctggg
     4261 ctacttcttc gacgaccacg tgcggttcat ggacgctttc atcgaggccc tgggactgga
     4321 agaggtggtg ctggtcatcc acgattgggg aagcgctctg ggcttccatt gggccaagag
     4381 gaacccagag agggtcaagg gcatcgcctt catggagttc atccggccta tccccacttg
     4441 ggacgagtgg ccagaattcg ccagggagac cttccaggct ttcagaacca ccgacgtggg
     4501 caggaagctg atcatcgacc agaacgtgtt catcgaggga accctgccta tgggagtcgt
     4561 gagacctctg accgaggtgg agatggacca ctacagggag cccttcctga acccagtgga
     4621 cagagagcct ctgtggagat tccccaacga gctgcctatc gccggagaac cagccaatat
     4681 cgtggccctg gtggaggagt acatggattg gctgcaccag agccccgtgc ctaagctgct
     4741 gttttggggc acaccaggag tgctgattcc tccagcagaa gccgctagac tggccaaaag
     4801 cctgcctaat tgcaaggccg tggacatcgg cccaggactg aatctgctgc aggaggacaa
     4861 ccccgacctg attggaagcg agattgcccg gtggctcagt accctggaga tcagcggcag
     4921 cgctggctct gctggagaca tgagagctgc caacctttgg ccaagcccgc tcatgatcaa
     4981 acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg tcagtgcctt
     5041 gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac ccttcagtga
     5101 agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc acatgatcaa
     5161 ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg tccaccttct
     5221 agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca tggagcaccc
     5281 agtgaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa aatgtgtaga
     5341 gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc gcatgatgaa
     5401 tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt ctggagtgta
     5461 cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc accgagtcct
     5521 ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga ccctgcagca
     5581 gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc acatgagtaa
     5641 caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc tctatgacct
     5701 gctgctggag atgctggacg cccaccgcct acatgcgccc actagccgtg gaggggcatc
     5761 cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat cgggtaccgt
     5821 caagcggccc atgaatgcct tcatggtgtg gtcccgcggg cagcggcgca agatggccca
     5881 ggagaacccc aagatgcaca actcggagat cagcaagcgc ctgggcgccg agtggaaact
     5941 tttgtcggag acggagaagc ggccgttcat cgacgaggct aagcggctgc gagcgctgca
     6001 catgaaggag cacccggatt ataaaggaag cggagctgga aagcctatcc ctaaccctct
     6061 gctgggactg gacagcacct gaataggtcg acaatcaacc tctggattac aaaatttgtg
     6121 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6181 taatgccttt gtatcatgcg ttaactaaac ttgtttattg cagcttataa tggttacaaa
     6241 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6301 ggtttgtcca aactcatcaa tgtatcttat catgtctgga attgactcaa atgatgtcaa
     6361 ttagtctatc agaagctcat ctggtctccc ttccggggga caagacatcc ctgtttaata
     6421 tttaaacagc agtgttccca aactgggttc ttatatccct tgctctggtc aaccaggttg
     6481 cagggtttcc tgtcctcaca ggaacgaagt ccctaaagaa acagtggcag ccaggtttag
     6541 ccccggaatt gactggattc cttttttagg gcccattggt atggcttttt ccccgtatcc
     6601 ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg
     6661 ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg cggggggagc
     6721 gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg gagggacgta
     6781 attacatccc tgggggcttt gggggggggc tgtccctgat atctataaca agaaaatata
     6841 tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat cgtatgagtt
     6901 aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc ggtcgttata
     6961 gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc tccaagcggc
     7021 gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga gagagcaata
     7081 tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa tctagctgca
     7141 tcaggatcat atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct
     7201 gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga
     7261 gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat
     7321 gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg
     7381 ggataccagt tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat
     7441 cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga
     7501 cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct ggatgccgca
     7561 gaaatggaca tggatacccc gtgagttacc cggcgggcgc gcttggcgta atcatggtca
     7621 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7681 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7741 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7801 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7861 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7921 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7981 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8041 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8101 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8161 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8221 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8281 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8341 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8401 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     8461 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8521 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8581 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8641 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8701 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8761 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8821 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8881 ggcttaccat ctggccccag tgctgcaatg ataccgcgag
//
