LOCUS       sequence-486445-        7985 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Expression of synaptic GFP and cytosolic mRuby.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7985)
  TITLE     Dual FingR and Cytosolic Marker
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EF1a-FLEX-mRuby-p2A-Gephyrin.FR-GFP-CCR5TC
FEATURES             Location/Qualifiers
     source          1..7985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     protein_bind    145..156
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     promoter        168..1346
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          399..1337
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1294..1314
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1363..1382
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1363..1381
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(1444..1477)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1528..1561)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1576..1701)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the rat zinc finger protein Kid-1 
                     (Witzgall et al., 1994)"
                     /label=KRAB-A
                     /translation="VSVTFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASM"
     CDS             complement(1750..2091)
                     /codon_start=1
                     /product="zinc-finger domain from the CCR5-224 (+) 
                     zinc-finger nuclease that recognizes the left half-site 
                     target sequence in the human CCR5 gene (Perez et al., 2008;
                     Gross et al., 2013)"
                     /label=CCR5TC
                     /translation="FQCRICMRNFSDRSNLSRHIRTHTGEKPFACDICGRKFAISSNLN
                     SHTKIHTGSQKPFQCRICMRNFSRSDNLARHIRTHTGEKPFACDICGRKFATSGNLTRH
                     TKIHLRGSQL"
     CDS             join(2128..2130,2131..2838)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     CDS             join(2128..2130,2131..2838)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     primer_bind     complement(2170..2191)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2431..2450)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2778..2799
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(2875..2898)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3166..3222)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(3238..3948)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     regulatory      3945..3954
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    3970..4003
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    4054..4087
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4112..4700
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4165..4185)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4583..4594)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4732..5208
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4931..4950)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   5248..5388
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5248..5377
                     /label=AAV2 ITR
     rep_origin      5463..5918
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5550..5569)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5760..5781
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5935..5954)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6054..6076
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6114..6132)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6200..6304
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6305..6373,6374..7165)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6523..6542)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7336..7924
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7825..7844
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcatc ctcatcgaat tccgtgaggc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
      421 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
      481 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
      541 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
      601 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
      661 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
      721 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
      781 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
      841 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
      901 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
      961 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     1021 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     1081 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     1141 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     1201 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     1261 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     1321 tttttcttcc atttcaggtg tcgtgaggaa ttagcttggt actaatacga ctcactatag
     1381 ggagacccaa gctggctagg taagcttggt accgagctcg gatcctctag agtcgactcc
     1441 ggaataactt cgtataggat actttatacg aagttatgca gaatggtagc tggattgtag
     1501 ctgctattag caatatgaaa cctcttaata acttcgtata gcatacatta tacgaagtta
     1561 tggcgcgcct taagccatag aagcaagatt agaataattt tcaagcataa cttcacgata
     1621 aagagaacgt tgagaaagat caagtttttt ccattcatca cgagtaaaaa gaacagcaac
     1681 atcttcaaaa gtaacagaaa cagaaacttg agaagcacct tcacgttgtt caggagcgtc
     1741 gacaatcgat agctggctgc cccgcaggtg aatctttgtg tgccgggtca gattgccgga
     1801 ggtggcaaac tttctgccac agatatcaca ggcaaaaggt ttttcaccag tatgtgtccg
     1861 gatgtgtctg gccaggttgt cgcttctgct aaagtttctc atacaaattc tgcactggaa
     1921 aggtttctgg ctgccagtgt ggatcttggt gtggctgttc aggttgctgc tgatggcgaa
     1981 cttccggccg caaatgtcgc aggcgaaggg cttttctccg gtgtgggttc tgatgtgcct
     2041 gctcaggttg gaccggtcgc tgaagttccg catgcagatc cggcactgga aagtaccacc
     2101 tgaacctcca gtaccacctg aacctccgtg agcaagggcg aggagctgtt caccggggtg
     2161 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     2221 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     2281 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     2341 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     2401 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     2461 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     2521 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     2581 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     2641 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     2701 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     2761 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     2821 ggcatggacg agctgtacag taccacctga acctccagta ccacctgaac ctcccttatc
     2881 gtcgtcatcc ttgtaatcgg atccggtgcg gtagttgatg gagatcggga tcgggtcctg
     2941 gtagttccag tggtcgatcg tgacggcgta caccgtgatg gtatagtcga caccaggttt
     3001 caggctgctg atggtagcag tggacttgct gccaggcacg gtgaattcct ggacagggct
     3061 attgccacca gtttcaccgt aggtgatgcg gtagtagcga accatgacct tgtacttgcc
     3121 ccagctgatc tggatgctgg ttggtgatgc ttccttgact tcgaggggac cggggttttc
     3181 ttccacgtct cctgcttgct ttaacagaga gaagttcgtg gctccggagc ccacaccctt
     3241 gtacagctcg tccatcccac caccaagccc ggcgaacttg gcaactgcgt gttcgcgttg
     3301 tactacgaac atttcattgt cactttcctc taacctttcc aggcggtgat caacggcatg
     3361 gataccgggc atcttgatgt tcccgacggt cttttttgac ctgtaagttg ttacgaaaga
     3421 gcaagacaga tggccaccac catcaacttt cagtgccata tgagtgtatc ccctcagacc
     3481 accatctgct ggatacatca tctctgtatt aggctcccaa cccttggtct tcttctgcat
     3541 cacgggacca ttggagggaa agtttacccc tctgacttgg acgtggtaaa cgagacagcc
     3601 atcctcaagg ctggtgtcct gcatgacggt gacgactcca ccatcttcgt atctcgtaac
     3661 tctttcccaa gtaaaaccct caggaaagga ctgtttaaag aaatcaggaa tgcctttcgg
     3721 gtacttgata aaagtacggc tgccatacat gaacgacgtg gcaagaatgt caaaggcaaa
     3781 tggcaggggt cctccctcga tgactttgat cctcatggtt tgagttccca tgtacggatt
     3841 gccttctcct tcacctgtgc atttgaattg gtggccgttg accgaacctt ccatgaccac
     3901 cttcatacgc atattttcct tgatcagctc ttcgccctta gacaccatgg tggcggcggt
     3961 ggcgctagca taacttcgta taaagtatcc tatacgaagt tatttgcctt aacccagaaa
     4021 ttatcactgt tattctttag aatggtgcaa agaataactt cgtataatgt atgctatacg
     4081 aagttatgaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     4141 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4201 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4261 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4321 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4381 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4441 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4501 ggggaaatca tcgtcctttc cttggctgct cgcctatgtt gccacctgga ttctgcgcgg
     4561 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4621 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     4681 cctttgggcc gcctccccgc atcgataccg agcgctgctc gagagatcta cgggtggcat
     4741 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     4801 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     4861 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     4921 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     4981 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
     5041 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     5101 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     5161 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttgtag
     5221 gtaaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct
     5281 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     5341 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
     5401 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     5461 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5521 cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5581 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5641 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     5701 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5761 tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt
     5821 ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt
     5881 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
     5941 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     6001 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     6061 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     6121 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     6181 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6241 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6301 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6361 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6421 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6481 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6541 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6601 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6661 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6721 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6781 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6841 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6901 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6961 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7021 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7081 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7141 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7201 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7261 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7321 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7381 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7441 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7501 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7561 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7621 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7681 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7741 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7801 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7861 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7921 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7981 catgt
//
