LOCUS       sequence-486446-        7988 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Expression of synaptic GFP and cytosolic mRuby.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7988)
  TITLE     Dual FingR and Cytosolic Marker
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EF1a-FLEX-Gephyrin.FR-GFP-CCR5TC-p2A-mRuby
FEATURES             Location/Qualifiers
     source          1..7988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     protein_bind    145..156
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     promoter        168..1346
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          399..1337
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1294..1314
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1363..1382
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1363..1381
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(1444..1477)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1528..1561)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1573..2283)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     CDS             complement(2284..2340)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2359..2484)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the rat zinc finger protein Kid-1 
                     (Witzgall et al., 1994)"
                     /label=KRAB-A
                     /translation="VSVTFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASM"
     CDS             complement(2533..2874)
                     /codon_start=1
                     /product="zinc-finger domain from the CCR5-224 (+) 
                     zinc-finger nuclease that recognizes the left half-site 
                     target sequence in the human CCR5 gene (Perez et al., 2008;
                     Gross et al., 2013)"
                     /label=CCR5TC
                     /translation="FQCRICMRNFSDRSNLSRHIRTHTGEKPFACDICGRKFAISSNLN
                     SHTKIHTGSQKPFQCRICMRNFSRSDNLARHIRTHTGEKPFACDICGRKFATSGNLTRH
                     TKIHLRGSQL"
     CDS             join(2911..2913,2914..3621)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     CDS             join(2911..2913,2914..3621)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     primer_bind     complement(2953..2974)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3214..3233)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3561..3582
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(3658..3681)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    3973..4006
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    4057..4090
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4115..4703
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4168..4188)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4586..4597)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4735..5211
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4934..4953)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   5251..5391
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5251..5380
                     /label=AAV2 ITR
     rep_origin      5466..5921
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5553..5572)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5763..5784
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5938..5957)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6057..6079
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6117..6135)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6203..6307
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6308..6376,6377..7168)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6526..6545)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7339..7927
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7828..7847
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcatc ctcatcgaat tccgtgaggc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
      421 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
      481 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
      541 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
      601 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
      661 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
      721 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
      781 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
      841 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
      901 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
      961 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     1021 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     1081 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     1141 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     1201 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     1261 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     1321 tttttcttcc atttcaggtg tcgtgaggaa ttagcttggt actaatacga ctcactatag
     1381 ggagacccaa gctggctagg taagcttggt accgagctcg gatcctctag agtcgactcc
     1441 ggaataactt cgtataggat actttatacg aagttatgca gaatggtagc tggattgtag
     1501 ctgctattag caatatgaaa cctcttaata acttcgtata gcatacatta tacgaagtta
     1561 tggcgcgcct tacttgtaca gctcgtccat cccaccacca agcccggcga acttggcaac
     1621 tgcgtgttcg cgttgtacta cgaacatttc attgtcactt tcctctaacc tttccaggcg
     1681 gtgatcaacg gcatggatac cgggcatctt gatgttcccg acggtctttt ttgacctgta
     1741 agttgttacg aaagagcaag acagatggcc accaccatca actttcagtg ccatatgagt
     1801 gtatcccctc agaccaccat ctgctggata catcatctct gtattaggct cccaaccctt
     1861 ggtcttcttc tgcatcacgg gaccattgga gggaaagttt acccctctga cttggacgtg
     1921 gtaaacgaga cagccatcct caaggctggt gtcctgcatg acggtgacga ctccaccatc
     1981 ttcgtatctc gtaactcttt cccaagtaaa accctcagga aaggactgtt taaagaaatc
     2041 aggaatgcct ttcgggtact tgataaaagt acggctgcca tacatgaacg acgtggcaag
     2101 aatgtcaaag gcaaatggca ggggtcctcc ctcgatgact ttgatcctca tggtttgagt
     2161 tcccatgtac ggattgcctt ctccttcacc tgtgcatttg aattggtggc cgttgaccga
     2221 accttccatg accaccttca tacgcatatt ttccttgatc agctcttcgc ccttagacac
     2281 catgggaccg gggttttctt ccacgtctcc tgcttgcttt aacagagaga agttcgtggc
     2341 tccggagccc acaccagcca tagaagcaag attagaataa ttttcaagca taacttcacg
     2401 ataaagagaa cgttgagaaa gatcaagttt tttccattca tcacgagtaa aaagaacagc
     2461 aacatcttca aaagtaacag aaacagaaac ttgagaagca ccttcacgtt gttcaggagc
     2521 gtcgacaatc gatagctggc tgccccgcag gtgaatcttt gtgtgccggg tcagattgcc
     2581 ggaggtggca aactttctgc cacagatatc acaggcaaaa ggtttttcac cagtatgtgt
     2641 ccggatgtgt ctggccaggt tgtcgcttct gctaaagttt ctcatacaaa ttctgcactg
     2701 gaaaggtttc tggctgccag tgtggatctt ggtgtggctg ttcaggttgc tgctgatggc
     2761 gaacttccgg ccgcaaatgt cgcaggcgaa gggcttttct ccggtgtggg ttctgatgtg
     2821 cctgctcagg ttggaccggt cgctgaagtt ccgcatgcag atccggcact ggaaagtacc
     2881 acctgaacct ccagtaccac ctgaacctcc gtgagcaagg gcgaggagct gttcaccggg
     2941 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     3001 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     3061 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     3121 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     3181 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     3241 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     3301 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     3361 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     3421 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     3481 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     3541 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     3601 ctcggcatgg acgagctgta cagtaccacc tgaacctcca gtaccacctg aacctccctt
     3661 atcgtcgtca tccttgtaat cggatccggt gcggtagttg atggagatcg ggatcgggtc
     3721 ctggtagttc cagtggtcga tcgtgacggc gtacaccgtg atggtatagt cgacaccagg
     3781 tttcaggctg ctgatggtag cagtggactt gctgccaggc acggtgaatt cctggacagg
     3841 gctattgcca ccagtttcac cgtaggtgat gcggtagtag cgaaccatga ccttgtactt
     3901 gccccagctg atctggatgc tggttggtga tgcttccttg acttcgagca tggtggcggc
     3961 ggtggcgcta gcataacttc gtataaagta tcctatacga agttatttgc cttaacccag
     4021 aaattatcac tgttattctt tagaatggtg caaagaataa cttcgtataa tgtatgctat
     4081 acgaagttat gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     4141 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     4201 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     4261 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     4321 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     4381 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     4441 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     4501 gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg
     4561 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     4621 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     4681 ctccctttgg gccgcctccc cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg
     4741 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc
     4801 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat
     4861 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt
     4921 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact
     4981 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga
     5041 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc
     5101 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct
     5161 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgattttg
     5221 taggtaacca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
     5281 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     5341 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     5401 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     5461 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5521 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
     5581 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5641 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     5701 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5761 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
     5821 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
     5881 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     5941 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     6001 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     6061 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     6121 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     6181 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6241 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6301 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6361 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6421 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     6481 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     6541 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     6601 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     6661 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     6721 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     6781 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     6841 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6901 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6961 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7021 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7081 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7141 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7201 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7261 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7321 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7381 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7441 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     7501 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7561 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7621 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7681 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7741 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7801 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7861 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7921 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7981 tcacatgt
//
