LOCUS       sequence-486469-       20468 bp    DNA     circular SYN 17-SEP-2025
DEFINITION  for chromosome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20468)
  AUTHORS   Sun C, Li H, Liu Y, Li Y, Gao R, Shi X, Fei H, Liu J, Liang R, Gao C
  TITLE     Iterative recombinase technologies for efficient and precise genome 
            engineering across kilobase to megabase scales.
  JOURNAL   Cell. 2025 Aug 1:S0092-8674(25)00800-1. doi: 
            10.1016/j.cell.2025.07.011.
  PUBMED    40763736
REFERENCE   2  (bases 1 to 20468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 1:S0092-8674(25)00800-1. doi: 10.1016/j.cell.2025.07.011."
COMMENT     Sequence Label: pH-SpRY-PCE
FEATURES             Location/Qualifiers
     source          1..20468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     233..255
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     247..264
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     248..264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     ncRNA           1414..1481
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     terminator      1512..1760
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        1783..3775
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     regulatory      3783..3792
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3796..3822
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             3862..3882
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8206..8226
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9952..9972
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11098..11124
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             11164..11184
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(11843..11859)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11843..11859)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11856..11878)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11867..11883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11891..11897,11898..11915,11916..11921))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11936..11957
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12148..12824
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     12761..12781
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             12891..13916
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    13957..14131
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    14209..14233
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             14658..15452
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      15539..16127
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16028..16047
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16281..16298
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    16313..16453
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(16379..16398)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     16539..16561
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      16797..16991
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(17057..18130)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(18559..19188)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
      121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      241 cgacgttgta aaacgacggc cagtgaattc atggagtcaa agattcaaat agaggaccta
      301 acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac
      361 aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc
      421 aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc
      481 ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa
      541 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
      601 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
      661 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgattggca gacatactgt
      721 cccacaaatg aagatggaat ctgtaaaaga aaacgcgtga aataatgcgt ctgacaaagg
      781 ttaggtcggc tgcctttaat caataccaaa gtggtcccta ccacgatgga aaaactgtgc
      841 agtcggtttg gctttttctg acgaacaaat aagattcgtg gccgacaggt gggggtccac
      901 catgtgaagg catcttcaga ctccaataat ggagcaatga cgtaagggct tacgaaataa
      961 gtaagggtag tttgggaaat gtccactcac ccgtcagtct ataaatactt agcccctccc
     1021 tcattgttaa gggagcaaaa tctcagagag atagtcctag agagagaaag agagcaagta
     1081 gcctagaagt agtcaaggcg gcgaagtatt caggcacgtg gccaggaaga agaaaagcca
     1141 agacgacgaa aacaggtaag agctaagcat ctagataagt tgaaaacaat cttcaaaagt
     1201 cccacatcgc ttagataaga aaacgaagct gagtttatat acagctagag tcgaagtagt
     1261 gattgaacaa agcaccagtg gtctagtggt agaatagtac cctgccacgg tacagacccg
     1321 ggttcgattc ccggctggtg catgagacct tcggggaata tgagcgtcag gtctcacgcg
     1381 gttctatcta gttacgcgtt aaaccaacta gaaggccggc atggtcccag cctcctcgct
     1441 ggcgccggct gggcaacatg cttcggcatg gcgaatggga cttttttttg atatctccgg
     1501 ggctaattga atatgaagat gaagatgaaa tatttggtgt gtcaaataaa aagctggtgt
     1561 gcttaagttt gtgttttttt cttggcttgt tgtgttatga atttgtggct ttttctaata
     1621 ttaaatgaat gtaagatctc attataatga ataaacaaat gtttctataa tccattgtga
     1681 atgttttgtt ggatctcttc tgcagcatat aactactgta tgtgctatgg tatggactat
     1741 ggaatatgat taaagataag aagcttggcg taatctgcat gcctgcagtg cagcgtgacc
     1801 cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa aaaattacca
     1861 catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata catatattta
     1921 aactttactc tacgaataat ataatctata gtactacaat aatatcagtg ttttagagaa
     1981 tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg acaacaggac
     2041 tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa atagcttcac
     2101 ctatataata cttcatccat tttattagta catccattta gggtttaggg ttaatggttt
     2161 ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta aattaagaaa
     2221 actaaaactc tattttagtt tttttattta ataatttaga tataaaatag aataaaataa
     2281 agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg aaacattttt
     2341 cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta acggacacca
     2401 accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg catctctgtc
     2461 gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc cgctgtcggc
     2521 atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg gcctcctcct
     2581 cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc gctttccctt
     2641 cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg
     2701 ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct
     2761 tcaaggtacg ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc
     2821 cggtccatgg ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt
     2881 tgtgttagat ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt
     2941 ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc
     3001 gcagacggga tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt
     3061 ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt
     3121 ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc
     3181 tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat
     3241 tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt
     3301 gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg
     3361 tggtgtggtt gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta
     3421 cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga
     3481 gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact
     3541 gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta
     3601 tctattataa taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg
     3661 gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc
     3721 ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta
     3781 gggccaccat ggatgcctgc cgccaagaga gtcaagctcg atggcggcaa gaggaccgcc
     3841 gatggcagcg agttcgagtc tcctaagaag aagaggaagg tcgacaagaa gtactcgatc
     3901 ggcctcgata ttgggactaa ctctgttggc tgggccgtga tcaccgacga gtacaaggtg
     3961 ccctcaaaga agttcaaggt cctgggcaac accgatcggc attccatcaa gaagaatctc
     4021 attggcgctc tcctgttcga cagcggcgag acggctgagc gtacgcggct caagcgcacc
     4081 gcccgcaggc ggtacacgcg caggaagaat cgcatctgct acctgcagga gattttctcc
     4141 aacgagatgg cgaaggttga cgattctttc ttccacaggc tggaggagtc attcctcgtg
     4201 gaggaggata agaagcacga gcggcatcca atcttcggca acattgtcga cgaggttgcc
     4261 taccacgaga agtaccctac gatctaccat ctgcggaaga agctcgtgga ctccacagat
     4321 aaggcggacc tccgcctgat ctacctcgct ctggcccaca tgattaagtt caggggccat
     4381 ttcctgatcg agggggatct caacccggac aatagcgatg ttgacaagct gttcatccag
     4441 ctcgtgcaga cgtacaacca gctcttcgag gagaacccca ttaatgcgtc aggcgtcgac
     4501 gcgaaggcta tcctgtccgc taggctctcg aagtctcgga agctcgagaa cctgatcgcc
     4561 cagctgccgg gcgagaagaa gaacggcctg ttcgggaatc tcattgcgct cagcctgggg
     4621 ctcacgccca acttcaagtc gaatttcgat ctcgctgagg acgccaagct gcagctctcc
     4681 aaggacacat acgacgatga cctggataac ctcctggccc agatcggcga tcagtacgcg
     4741 gacctgttcc tcgctgccaa gaatctgtcg gacgccatcc tcctgtctga tattctcagg
     4801 gtgaacaccg agattacgaa ggctccgctc tcagcctcca tgatcaagcg ctacgacgag
     4861 caccatcagg atctgaccct cctgaaggcg ctggtcaggc agcagctccc cgagaagtac
     4921 aaggagatct tcttcgatca gtcgaagaac ggctacgctg ggtacattga cggcggggcc
     4981 tctcaggagg agttctacaa gttcatcaag ccgattctgg agaagatgga cggcacggag
     5041 gagctgctgg tgaagctcaa gcgcgaggac ctcctgagga agcagcggac attcgataac
     5101 ggcagcatcc cacaccagat tcatctcggg gagctgcacg ctatcctgag gaggcaggag
     5161 gacttctacc ctttcctcaa ggataaccgc gagaagatcg agaagattct gactttcagg
     5221 atcccgtact acgtcggccc actcgctagg ggcaactccc gcttcgcttg gatgacccgc
     5281 aagtcagagg agacgatcac gccgtggaac ttcgaggagg tggtcgacaa gggcgctagc
     5341 gctcagtcgt tcatcgagag gatgacgaat ttcgacaaga acctgccaaa tgagaaggtg
     5401 ctccctaagc actcgctcct gtacgagtac ttcacagtct acaacgagct gactaaggtg
     5461 aagtatgtga ccgagggcat gaggaagccg gctttcctgt ctggggagca gaagaaggcc
     5521 atcgtggacc tcctgttcaa gaccaaccgg aaggtcacgg ttaagcagct caaggaggac
     5581 tacttcaaga agattgagtg cttcgattcg gtcgagatct ctggcgttga ggaccgcttc
     5641 aacgcctccc tggggaccta ccacgatctc ctgaagatca ttaaggataa ggacttcctg
     5701 gacaacgagg agaatgagga tatcctcgag gacattgtgc tgacactcac tctgttcgag
     5761 gaccgggaga tgatcgagga gcgcctgaag acttacgccc atctcttcga tgacaaggtc
     5821 atgaagcagc tcaagaggag gaggtacacc ggctggggga ggctgagcag gaagctcatc
     5881 aacggcattc gggacaagca gtccgggaag acgatcctcg acttcctgaa gagcgatggc
     5941 ttcgcgaacc gcaatttcat gcaactgatc cacgatgaca gcctcacctt caaggaggat
     6001 atccaaaagg ctcaagtgag cggccagggg gactcgctgc acgagcatat cgcgaacctc
     6061 gctggctccc ccgcgatcaa gaagggcatc ctccagaccg tgaaggttgt ggacgagctc
     6121 gtgaaggtca tgggccggca caagcctgag aacatcgtca tcgagatggc cagagagaac
     6181 caaaccacgc agaaggggca aaagaactct agggagcgca tgaagcgcat cgaggagggc
     6241 atcaaggagc tggggtccca aatcctcaag gagcacccag tggagaacac ccaactgcag
     6301 aacgagaagc tctacctgta ctacctccag aacggcaggg atatgtacgt ggaccaagag
     6361 ctggatatca accgcctcag cgattacgac gtcgatgcca tcgttcccca gtctttcctg
     6421 aaggatgact ccatcgacaa caaggtcctc accaggtcgg acaagaaccg cggcaagtca
     6481 gataacgttc catctgagga ggtcgttaag aagatgaaga actactggag gcagctcctg
     6541 aacgccaagc tgatcacgca aaggaagttc gacaacctca ccaaggctga gagaggcggg
     6601 ctctcagagc tggacaaggc cggcttcatc aagcggcagc tggtcgagac cagacaaatc
     6661 acgaagcacg ttgcgcaaat cctcgactct cggatgaaca cgaagtacga tgagaacgac
     6721 aagctgatca gggaggttaa ggtgatcacc ctgaagtcta agctcgtctc cgacttcagg
     6781 aaggatttcc agttctacaa ggttcgcgag atcaacaact accaccatgc ccatgacgct
     6841 tacctcaacg ctgtggtcgg caccgctctg atcaagaagt acccaaagct ggagtccgag
     6901 ttcgtgtacg gggactacaa ggtttacgat gtgcgcaaga tgatcgccaa gtcggagcaa
     6961 gagatcggca aggctaccgc caagtacttc ttctactcaa acatcatgaa cttcttcaag
     7021 accgagatca cgctggccaa cggcgagatc cggaagagac cgctcatcga gaccaacggc
     7081 gagacggggg agatcgtgtg ggacaagggc agggatttcg cgaccgtccg caaggttctc
     7141 tccatgcccc aggtgaacat cgtcaagaag accgaggtcc aaacgggcgg gttctcaaag
     7201 gagtctatca ggcctaagcg gaacagcgac aagctcatcg ccagaaagaa ggactgggac
     7261 ccaaagaagt acggcgggtt cctctggcct accgtggcct actcggtcct ggttgtggcg
     7321 aaggttgaga agggcaagtc caagaagctc aagagcgtga aggagctcct ggggatcacc
     7381 atcatggaga ggtccagctt cgagaagaac ccaatcgact tcctggaggc caagggctac
     7441 aaggaggtga agaaggacct gatcatcaag ctcccgaagt actctctctt cgagctggag
     7501 aacggcagga agagaatgct ggcttccgct aagcagctcc agaaggggaa cgagctcgcg
     7561 ctgccaagca agtacgtgaa cttcctctac ctggcttccc actacgagaa gctcaagggc
     7621 agcccggagg acaacgagca aaagcagctg ttcgtcgagc agcacaagca ttacctcgac
     7681 gagatcatcg agcaaatctc cgagttcagc aagcgcgtga tcctcgccga cgcgaacctg
     7741 gataaggtcc tctccgccta caacaagcac cgggacaagc ccatcagaga gcaagcggag
     7801 aacatcatcc atctcttcac cctgacgagg ctcggcgctc ctagggcttt caagtacttc
     7861 gacaccacga tcgatcccaa gcaatacagg tccacgaagg aggtcctgga cgcgaccctc
     7921 atccaccagt cgatcaccgg cctgtacgag acgaggatcg acctctcaca actcggcggg
     7981 gatgaattct ccgggagcga gacgccaggc acctccgagt cggccacccc agaatctgcc
     8041 acagtggtgt ccggccaaaa gcaggaccgc cagggcggag aacgcagaag gtcccagctc
     8101 gatagggatc agtgtgccta ctgcaaggag aagggccact gggccaaaga ctgcccgaaa
     8161 aagccgcgcg gcccacgcgg cccaaggcca caaacatccc tccttccaaa gaagaagcgg
     8221 aaggtggagc tcagcggagg atcttccgga ggatctagcg gctccgagac accaggaaca
     8281 tccgaaagcg ctacaccaga atctagcgga ggctcttccg gaggatctag gcctaccctc
     8341 aacatcgagg atgagtatcg cctccacgaa acctccaaag aaccggacgt gtccctcggc
     8401 agcacatggc tcagcgactt cccacaagcg tgggccgaaa ccggcggcat gggcctcgcc
     8461 gtccgccaag ccccactcat tatcccgctg aaggcgacct ccacaccggt gtccatcaag
     8521 cagtacccga tgagccaaga ggcgaggctc gggattaagc cgcacattca gcgcctcctc
     8581 gatcaaggca ttctcgtgcc gtgccaatcc ccgtggaata caccactcct cccggtcaaa
     8641 aagccgggca ccaacgacta tcgcccggtc caagatctcc gcgaggtcaa caagcgcgtg
     8701 gaagacatcc acccgaccgt cccgaacccg tataatctgc tctccgggct cccaccatcc
     8761 caccagtggt atacagtgct ggacctcaaa gacgccttct tctgtctccg cctccaccca
     8821 acaagccagc cgctcttcgc cttcgagtgg cgcgacccgg agatgggcat ctccggccaa
     8881 ctgacatgga cacgcctccc gcaaggcttc aagaacagcc cgacactctt caacgaggcg
     8941 ctccataggg acctcgcgga ttttcgcatc cagcatccgg acctcatcct cctccagtat
     9001 gcggatgatc tcctcctcgc cgcgacctcc gagctggatt gtcaacaagg cacacgcgcg
     9061 ctcctccaaa cactcgggaa cctcggctat cgcgcgtccg cgaaaaaggc ccaaatctgc
     9121 cagaagcaag tgaagtacct cgggtatctg ctcaaggaag gccaacgctg gctcaccgaa
     9181 gcgcgcaaag aaacagtgat ggggcaaccg acaccgaaaa caccacgcca gctgcgcgag
     9241 tttctcggca aagccggctt ctgtcgcctc ttcatcccgg gctttgccga gatggccgcg
     9301 ccactctacc cactcaccaa gccgggcaca ctgtttaact gggggccgga tcagcagaaa
     9361 gcctaccaag agatcaaaca agcgctcctc accgccccag cgctcgggct cccagatctc
     9421 acaaagccgt tcgagctgtt cgtcgatgag aagcaaggct acgcgaaggg cgtgctcaca
     9481 cagaagctcg gcccgtggag gaggccagtg gcctatctct ccaaaaaact cgatccagtg
     9541 gccgccggct ggccaccgtg tctgcgcatg gtcgccgcga ttgccgtgct cacaaaggat
     9601 gccggcaaac tcacaatggg ccagccgctg gtgatcctcg cgccacatgc cgtggaagcc
     9661 ctcgtcaaac agccgccgga taggtggctc tccaatgcgc gcatgaccca ttaccaagcg
     9721 ctcctcctcg acaccgatcg cgtccagttc ggcccagtgg tcgccctcaa tccggcgaca
     9781 ctgctgccac tcccagagga gggcctccaa cacaactgtc tggatattct cgcggaagcg
     9841 catggcacaa ggccagacct cacagatcaa ccgctcagcg gcggcagcaa gaggaccgcc
     9901 gactcccagc actcgacccc gccgaagacc aagaggaagg tggagttcga gccgaagaag
     9961 aagaggaagg tctccggcgg atcttccgga ggatctagcg gctccgagac accaggaaca
    10021 tccgaatccg ctacaccaga gtcttctgga ggatctagcg gaggatctat gagtaatctc
    10081 ttgaccgttc atcaaaattt accggcgctc ccggtggacg ccacaagcga tgaggtgcgg
    10141 aagaacctga tggatatgtt ccgtgatcgg caggcgttta gcgaagagac atggaagatg
    10201 cttctttccg tgtgtcgtag ttgggcagcg tggtgcaagc tcaacaaccg gaagtggttc
    10261 cctgctgagc cggaagacgt cagggactat ttactctacc tccaagctag ggggttagca
    10321 gttaaaacta tacagcagca tctcggtcaa ttgaacatgc tgcaccggcg cagcggactg
    10381 ccgcggccca gtgactctaa tgctgtgtcg ctcgtgatga ggcgcatcag aaaggagaat
    10441 gtggatgctg gtgaacgagc caagcaggcg cttgccttcg agaggacaca gtttgatcaa
    10501 gttcgcagcc tcatggagaa cagcgatcgt tgccaagaca tcaggaatct agccttcctg
    10561 ggtatcgcct acaatactct gttgagaatt gcggagattg cgaggatccg tgtgaaggac
    10621 ataagccgaa ctgatggtgg gaggatgttg attcacattg gacgcaccaa gacactcgta
    10681 tcaaccgcag gagtcgagaa ggctctatcg cttggggtta ctaagttggt cgaacgatgg
    10741 atatcagtat ccggcgtcgc cgacgatcca aacaattacc tgttctgccg cgtgcgcaag
    10801 aatggggttg cggcgccttc agctacgtcc cagctctcga cgagggcact ggagggcatc
    10861 tttgaggcta cgcacaggct gatctatggc ccgccggatg attccggcgg gagatacttg
    10921 gcatggtctg gacactccgc cagagtcggc gccgcccgcg acatggcgcg ggccggcgtg
    10981 tcaattccag aaataatgca ggcaggaggg tggaccaatg tcaacattgt aatgaactat
    11041 atcagaaacc tcgactcgga gacaggagcc atggtcaggc ttctagaaga cggtgaccca
    11101 gcagctaaac gcgtaaagtt ggatggaggt aagaggacag cggatggatc tgaattcgag
    11161 agcccgaaaa agaagaggaa agtctgagag ctcagagctt tcgttcgtat catcggtttc
    11221 gacaacgttc gtcaagttca atgcatcagt ttcattgcgc acacaccaga atcctactga
    11281 gtttgagtat tatggcattg ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat
    11341 ttactgtgtt ttttattcgg ttttcgctat cgaactgtga aatggaaatg gatggagaag
    11401 agttaatgaa tgatatggtc cttttgttca ttctcaaatt aatattattt gttttttctc
    11461 ttatttgttg tgtgttgaat ttgaaattat aagagatatg caaacatttt gttttgagta
    11521 aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat atgaggagta aaacacttgt
    11581 agttgtacca ttatgcttat tcactaggca acaaatatat tttcagacct agaaaagctg
    11641 caaatgttac tgaatacaag tatgtcctct tgtgttttag acatttatga actttccttt
    11701 atgtaatttt ccagaatcct tgtcagattc taatcattgc tttataatta tagttatact
    11761 catggatttg tagttgagta tgaaaatatt ttttaatgca ttttatgact tgccaattga
    11821 ttgacaacga attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca
    11881 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    11941 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    12001 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattggct
    12061 agagcagctt gccaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga
    12121 tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa
    12181 cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga
    12241 aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc
    12301 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
    12361 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgaa catggtggag cacgacactc
    12421 tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt
    12481 ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
    12541 tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag
    12601 gaaaggctat cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
    12661 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
    12721 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
    12781 cctctatata aggaagttca tttcatttgg agaggacacg ctgaaatcac cagtctctct
    12841 ctacaaatct atctctctcg agctttcgca gatccggggg gcaatgagat atgaaaaagc
    12901 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
    12961 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
    13021 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
    13081 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggagttta
    13141 gcgagagcct gacctattgc atctcccgcc gttcacaggg tgtcacgttg caagacctgc
    13201 ctgaaaccga actgcccgct gttctacaac cggtcgcgga ggctatggat gcgatcgctg
    13261 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
    13321 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
    13381 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
    13441 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
    13501 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
    13561 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
    13621 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgcca cgactccggg
    13681 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
    13741 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
    13801 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
    13861 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag aaatagagta
    13921 gatgccgacc gggatctgtc gatcgacaag ctcgagtttc tccataataa tgtgtgagta
    13981 gttcccagat aagggaatta gggttcctat agggtttcgc tcatgtgttg agcatataag
    14041 aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct
    14101 aaaaccaaaa tccagtacta aaatccagat cccccgaatt aattcggcgt taattcagta
    14161 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
    14221 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
    14281 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
    14341 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
    14401 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
    14461 gcctgtgatc accgcggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt
    14521 gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt
    14581 gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa
    14641 gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg
    14701 aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg
    14761 tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct
    14821 atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa
    14881 aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg
    14941 gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt
    15001 atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata
    15061 gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg
    15121 attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt
    15181 taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca
    15241 acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg
    15301 cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag
    15361 aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa
    15421 aatattatat tttactggat gaattgtttt agtacctaga atgcatgacc aaaatccctt
    15481 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    15541 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    15601 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    15661 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
    15721 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    15781 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    15841 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    15901 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    15961 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    16021 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    16081 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    16141 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    16201 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    16261 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
    16321 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
    16381 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
    16441 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
    16501 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
    16561 gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt
    16621 cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca
    16681 tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca
    16741 gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg
    16801 cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat
    16861 cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct
    16921 ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg
    16981 tgctatccac aggaaacaga ccttttcgac ctttttcccc tgctagggca atttgcccta
    17041 gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc
    17101 gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt
    17161 catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc
    17221 cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc
    17281 gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa
    17341 ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct
    17401 cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca
    17461 aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca
    17521 tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc
    17581 cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc
    17641 cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg
    17701 ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct
    17761 ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg
    17821 acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga
    17881 gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg
    17941 caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg
    18001 attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca
    18061 acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc
    18121 gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcaggc
    18181 aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc
    18241 gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct
    18301 actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct
    18361 cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg
    18421 gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag
    18481 ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt
    18541 tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac
    18601 ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt
    18661 gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc
    18721 cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa
    18781 ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc
    18841 ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc
    18901 cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc
    18961 gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt
    19021 tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc
    19081 gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt
    19141 cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg
    19201 cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt
    19261 tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg
    19321 gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg
    19381 gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc
    19441 tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct
    19501 cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga
    19561 aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt
    19621 acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct
    19681 ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa
    19741 acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg
    19801 aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg
    19861 tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct
    19921 cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca
    19981 caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg
    20041 tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt
    20101 ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg
    20161 cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac
    20221 taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt
    20281 aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg
    20341 cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa ccctgtggtt
    20401 ggcatgcaca tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat
    20461 tattctaa
//
