LOCUS       sequence-486493-        9284 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  The Mef2c anterior heart field promoter driving expression of a 
            tamoxifen inducible Dre recombinase. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9284)
  AUTHORS   Gillespie W, Zhang Y, Ruiz OE, Cerda J, Ortiz-Guzman J, Turner WD, 
            Largoza G, Sherman M, Mosser LE, Fujimoto E, Chien CB, Kwan KM, 
            Arenkiel BR, Devine WP, Wythe JD
  TITLE     Multisite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox with diverse applications in vertebrate model 
            systems.
  JOURNAL   bioRxiv [Preprint]. 2024 Jul 13:2024.07.13.603267. doi: 
            10.1101/2024.07.13.603267.
  PUBMED    39026881
REFERENCE   2  (bases 1 to 9284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jul 13:2024.07.13.603267. doi: 
            10.1101/2024.07.13.603267."
COMMENT     Sequence Label: pMEF2c-AHF-DreERT2 (JDW 196)
FEATURES             Location/Qualifiers
     source          1..9284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..41
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(260..364)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4445..4464
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    4504..4528
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4541..4550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5618..6553
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5666..6559
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     protein_bind    complement(6580..6604)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6696..6715)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6761..6816
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6815..6834)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7177..7193)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7177..7193)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7190..7212)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7201..7217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7225..7231,7232..7249,7250..7255))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7270..7291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7350..7545
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7396..7531
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7551..7685
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7588..7607)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7642..7661
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7753..7770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7924..8512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8004..8023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8683..9284,1..190,191..259))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
       61 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      121 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      181 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      241 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      301 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
      361 cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac tatcgagagg
      421 aaagccacca gtttctttgt cttgaagtat ttatttaaaa tgttggcatg tgtgacagtg
      481 atacagttaa atggccatgc tcttctttat gatatgtgca gtgctgtggc atggtggttg
      541 ctcagagctc ctaaaacctt ccaagggcca cctttgcaag tgcctactac aattaagctg
      601 attcctaggt ggccatgtac atcatcatag gacaaactac aaagaggtta aaatatcttt
      661 tacattatgg aatttgaatc ttaccatgtt tctcggattt ttatttttct aaattttcat
      721 gttcaattat atttatgatg aaagtattct ggctgaagaa caaatactat acacttgaat
      781 aatcttgagg ggtagattaa tctatatcct ttcttcatct cagggtaggt tgctgtggca
      841 accgccccag ctcactttaa aataattttt taaagctcac ttcaagagct acacccagcg
      901 tgggccaggc ttaaaagcag gcagacccag tgcatagggc tttcgtagac tgctgcttta
      961 atgtagggag gcagaagaaa gggagctgaa gtaagatggc tcaaaacaga aattggaatg
     1021 ttagctttga gctgaaacaa tcaatcacca tgctctctgt aacttcaggt gtctaaatgc
     1081 attgtgaaat aaatccttgg atgagataac taatgtttat ttatatttaa aattaagcac
     1141 ttataataat taatgaaagg aatatttgta attacacaca ttcagagaga gaaggggagg
     1201 gggggagaag gagagagagg aagtgagtga gtgattgaga gagagagaga gagagagagg
     1261 gagggaggga gagggaggta aggagaaagg tggtttaatg gcaggtcaat tctagccatc
     1321 acttcatctt ctttattaaa ggaaagtttc cttttattaa atccttagct tcactcccca
     1381 ctgatataaa agatataata attatgatta ttttcaagca tagaacaaaa ttggagagct
     1441 catgcaaacc aggaaaccga ggcctagaaa ggatttatct attaggaaga cacttttagt
     1501 gttttcaatg taaactatca aaccataagt aaaaaagtta gtagtgctct gccacagagt
     1561 cacatctaca accacaaatc ccaaactgga tagagcattc taagtctctc aacatctaga
     1621 acaaaatact tcctacacaa gaaaaatgaa taccattagg tagcaaggat caccagttta
     1681 ggtaaataaa agtaaagaga taatgtgtgt ctggcattac ttaaagacaa atataaaaca
     1741 cactctggat taacaatctc atggattact actaaattat ttacttattt atttacttat
     1801 atatatattt atttattttc cacttcagct acaaggtttg tgaggaaaac taacactaat
     1861 cttaactttg ctcacacatc attaagaagc taaatataca aagggggaaa atcttatctt
     1921 ttcaaaggcc agctgtaata cattccagac ccaaagtttc aaatcatcct cacctggact
     1981 ggacatactt ttaaatgttt acaatattgt tgataaatac gggtttgtta caatacttat
     2041 tactaaacaa ttctttgaca cctggtgggg aaaaataaaa gtttcctttt gaaggaaaat
     2101 aaatcaaaac tgaagtgtgc tgtggtaagt agaaactttg tctataatta aggagaagag
     2161 catgattgaa ttcatgtatt ggctatatca actctgtcat gtaacatagg ccattatagt
     2221 tcattatact aatttaaagc actatgagaa taagttctct tggtccttct tatagtttgc
     2281 tctgtgagga tctttgtaaa ccttaaaaat aatctaaatt tttatttata tcaccatatc
     2341 taggtatgcc ttagcatgca gcatgtctaa gctcagagct tgtacctcta aacatgccgt
     2401 tctgtttata taaactcatg gtagtcacac ttgctatgtg aaagaggagc aaatacaata
     2461 tagcatagat accattttca aaagcagaac aaacaaaaaa cccaccatgt cacaaagctt
     2521 gtgctctgtg acagcttgtc atgctttcct gtaataaaat cgcagtacta attaatagtc
     2581 tcagtggtaa ggagccagag gtttgaagat tgttcccatg tagtttaact tgcctgtaaa
     2641 atcaactttc tgctttcaaa tgaagccttc cttcggaggg tgcttaaatg ttgagtccac
     2701 tgctctgata gaagctacta acttcaaaca gcacaatgag gcaggaggct tttttgatgc
     2761 acaatgcccc tctgcattaa aatagtactc tgcaggccta ctggagagct tcccggggaa
     2821 ttcagccagt tactgggtta aacatatact ttcgaaaata tttttaatta gctagatatt
     2881 tctatgggct actattgtct actcttgtat cttctacatt atagtactct gtgaactttc
     2941 tcttcctcta atcatagtat taaaaattaa atgttcatat tttacacaag ctacagtact
     3001 cctggcatgc aatctttcac tattttgaga atgcataggg ttgacttgta tatagttgat
     3061 agatttaatc tttagctttt taggtgtggt cgggatatct agtacctttt ttttttctaa
     3121 gtatcaattc tttgttaacc tatattttta ctatggaaaa ttattcgaca gcttaaacct
     3181 taacctaaga gttcttatca gtgtctgctc ctgcttcctg tgcagctgaa actcttcagg
     3241 ataagtgaag tcacccgcta tctatcggtc aggggagcct aatgcatttg ggaagttgtt
     3301 tttggtttac ttgctaatga cctggataaa gtccagtaag gtgtggaggg aaggaggggg
     3361 cattctccga ggaagctgtc ctctcagtaa atttgtccag attgattcga aagttaatgg
     3421 cccggggata aagtcgagat ttcaaaaact tgtaattaat ttattactaa cattggagga
     3481 tccattcggc tcactttcta gcctagccct cctgactctc aaggccaaaa gctttataca
     3541 ttctgggcgg gcaggaagcg cacgctggga tcgaggcttg cagacatcct ggatcgtggc
     3601 ttttacgcgt gagtaacgga ggcgcgcgcg gaccggagca aggatgaagt ggctactgag
     3661 aatctaaagg gggcgggggg gggggggagt cactagagca gctcttaggg tcgcgctcct
     3721 attggtcaga aggaagatct cgacttcaga aggactttcc tgctagctgg cttgcagcct
     3781 ccccggggga gcgagcgcgc aggagactag cgtcctctgt agcggcgggg agagaggcag
     3841 ggagccgccg ctccccgtgt tgtcttccct ttcatgcaat aaagaattgt cattaggttt
     3901 gcgtatggca tgtgccatta ccccaccgta aaactaaaat tagcaaaatg tcaggaatgg
     3961 aaagatcgat tcaccaagat gcaattatca tttaaaagtg cttgattgag gtactgatgt
     4021 tcagtgattt attctgcata ccatatacat aattaaagta gtgtagtgga gtaatttatc
     4081 aatctagttg agactggagg ggtgaggagg gagctgcagt atgtttgttt tattaaaatg
     4141 ttgcgaggtt ctagtcccgc cccccttttg caagagtgaa actgatgatt tccccagctc
     4201 gggaggagag gcaacggttt gggctggttg ggatagagaa acggaaagga aaaggcagcg
     4261 ccaggcaaag gcaagaataa aatgaattgg gggacggggg tagcaccgga gcgtacgtgc
     4321 tgcttagaac cccagccccg tgtgctccgt cttgcttgcc gcccccgcgt cccctcccag
     4381 tcgaggtcga ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     4441 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cctcgaggat
     4501 atcacaagtt tgtacaaaaa agcaggctga attcgtcgac gccaccatgg gtgctagcga
     4561 gctgatcatc tctggctcct ctggaggatt cctgaggaac atcggcaagg agtaccagga
     4621 ggctgctgag aacttcatga gattcatgaa tgaccaggga gcctacgccc ctaacaccct
     4681 gagagacctg aggctggtgt tccactcctg ggctagatgg tgccacgcta gacagctggc
     4741 ctggttccct atctctcctg agatggctag ggagtacttc cttcagctgc acgatgctga
     4801 cctggcctct accaccatcg acaagcacta cgccatgctg aacatgctgc tgtcccactg
     4861 tggcctgcct cctctgtctg atgacaagtc tgtgagcctg gccatgagga gaatccggag
     4921 agaggctgcc accgagaagg gagagagaac cggccaggcc atccctctga gatgggatga
     4981 cctgaagctg ctggatgtgc tgctgtctag atctgagaga ctggtggacc tgaggaatag
     5041 ggccttcctg tttgtggcct acaacaccct gatgaggatg tctgagatct ctaggatcag
     5101 agtgggagac ctggaccaga ccggagacac cgtgaccctg cacatctccc acaccaagac
     5161 catcaccacc gctgctggcc tggacaaagt gctgtctagg aggaccaccg ctgtgctgaa
     5221 tgactggctg gatgtgtctg gcctgagaga gcaccctgac gctgtgctgt tccctcctat
     5281 ccaccggagc aacaaggcta ggatcaccac cacccctctg accgcccctg ccatggagaa
     5341 gatttttagc gatgcctggg tgctgctgaa caagagggat gccaccccta acaagggccg
     5401 ctaccggacc tggaccggcc actctgctag agtgggagct gccatcgaca tggctgagaa
     5461 gcaagtgtcc atggtggaga tcatgcagga gggcacctgg aaaaagcctg agacactgat
     5521 gagatacctg aggaggggag gagtgtctgt gggagccaac tctaggctga tggactccgc
     5581 tagcggcgcc agacgcatat gcgtacgcgg atcctctgct ggagacatga gagctgccaa
     5641 cctttggcca agcccgctca tgatcaaacg ctctaagaag aacagcctgg ccttgtccct
     5701 gacggccgac cagatggtca gtgccttgtt ggatgctgag ccccccatac tctattccga
     5761 gtatgatcct accagaccct tcagtgaagc ttcgatgatg ggcttactga ccaacctggc
     5821 agacagggag ctggttcaca tgatcaactg ggcgaagagg gtgccaggct ttgtggattt
     5881 gaccctccat gatcaggtcc accttctaga atgtgcctgg ctagagatcc tgatgattgg
     5941 tctcgtctgg cgctccatgg agcacccagt gaagctactg tttgctccta acttgctctt
     6001 ggacaggaac cagggaaaat gtgtagaggg catggtggag atcttcgaca tgctgctggc
     6061 tacatcatct cggttccgca tgatgaatct gcagggagag gagtttgtgt gcctcaaatc
     6121 tattattttg cttaattctg gagtgtacac atttctgtcc agcaccctga agtctctgga
     6181 agagaaggac catatccacc gagtcctgga caagatcaca gacactttga tccacctgat
     6241 ggccaaggca ggcctgaccc tgcagcagca gcaccagcgg ctggcccagc tcctcctcat
     6301 cctctcccac atcaggcaca tgagtaacaa aggcatggag catctgtaca gcatgaagtg
     6361 caagaacgtg gtgcccctct atgacctgct gctggaggcg gcggacgccc accgcctaca
     6421 tgcgcccact agccgtggag gggcatccgt ggaggagacg gaccaaagcc acttggccac
     6481 tgcgggctct acttcatcgc attccttgca aaagtattac atcacggggg aggcagaggg
     6541 tttccctgcc acagcttgag cggccgcctc gaggaattca cccagctttc ttgtacaaag
     6601 tggtgatatc gcggccgcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     6661 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     6721 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     6781 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     6841 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     6901 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     6961 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     7021 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     7081 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     7141 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7201 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7261 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7321 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg
     7381 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7441 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7501 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta
     7561 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7621 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7681 ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7741 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7801 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7861 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7921 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7981 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8041 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8101 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8161 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8221 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8281 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8341 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8401 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8461 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8521 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8581 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8641 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8701 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8761 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8821 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8881 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8941 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9001 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9061 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9121 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9181 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9241 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgct
//
