LOCUS       sequence-486497-        9306 bp    DNA     circular SYN 16-SEP-2025
DEFINITION  Mammalian expression of hsOPA1 isoform 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Song Z, Chen H, Fiket M, Alexander C, Chan DC
  TITLE     OPA1 processing controls mitochondrial fusion and is regulated by 
            mRNA splicing, membrane potential, and Yme1L.
  JOURNAL   J Cell Biol. 2007 Aug 27;178(5):749-55. Epub 2007 Aug 20.
  PUBMED    17709429
REFERENCE   2  (bases 1 to 9306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2007-08-27"; volume: "178(5)"; pages: "749-55. Epub 
            2007 Aug 20"
COMMENT     Sequence Label: OPA1-isoform1
FEATURES             Location/Qualifiers
     source          1..9306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     polyA_signal    4347..4468
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4358..4377)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4412..4431
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4482..4981
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4517..4540)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4914..4933
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5002..5601
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5002..5021)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5498..5518
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5701..6215
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6377..6393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6401..6407,6408..6425,6426..6431))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6446..6467
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6584..6601)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6755..7343)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6835..6854)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7514..8305,8306..8374))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8137..8156
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8375..8479)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8547..8565
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8603..8625)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8725..8744
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8938..8960
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9088..9107
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatctctc gagaattcac gcgtggtacc
     1441 atgtggcgac tacgtcgggc cgctgtggcc tgtgaggtct gccagtcttt agtgaaacac
     1501 agctctggaa taaaaggaag tttaccacta caaaaactac atctggtttc acgaagcatt
     1561 tatcattcac atcatcctac cttaaagctt caacgacccc aattaaggac atcctttcag
     1621 cagttctctt ctctgacaaa ccttccttta cgtaaactga aattctctcc aattaaatat
     1681 ggctaccagc ctcgcaggaa tttttggcca gcaagattag ctacgagact cttaaaactt
     1741 cgctatctca tactaggatc ggctgttggg ggtggctaca cagccaaaaa gacttttgat
     1801 cagtggaaag atatgatacc ggaccttagt gaatataaat ggattgtgcc tgacattgtg
     1861 tgggaaattg atgagtatat cgattttgag aaaattagaa aagcccttcc taattcagaa
     1921 gaccttgtaa agttagcacc agactttgac aagattgttg aaagccttag cttattgaag
     1981 gactttttta cctcaggttc tccggaagaa acggcgttta gagcaacaga tcgtggatct
     2041 gaaagtgaca agcattttag aaaggtgtca gacaaagaga aaattgacca acttcaggaa
     2101 gaacttctgc acactcagtt gaagtatcag agaatcttgg aacgattaga aaaggagaac
     2161 aaagaattga gaaaattagt attgcagaaa gatgacaaag gcattcatca tagaaagctt
     2221 aagaaatctt tgattgacat gtattctgaa gttcttgatg ttctctctga ttatgatgcc
     2281 agttataata cgcaagatca tctgccacgg gttgttgtgg ttggagatca gagtgctgga
     2341 aagactagtg tgttggaaat gattgcccaa gctcgaatat tcccaagagg atctggggag
     2401 atgatgacac gttctccagt taaggtgact ctgagtgaag gtcctcacca tgtggcccta
     2461 tttaaagata gttctcggga gtttgatctt accaaagaag aagatcttgc agcattaaga
     2521 catgaaatag aacttcgaat gaggaaaaat gtgaaagaag gctgtaccgt tagccctgag
     2581 accatatcct taaatgtaaa aggccctgga ctacagagga tggtgcttgt tgacttacca
     2641 ggtgtgatta atactgtgac atcaggcatg gctcctgaca caaaggaaac tattttcagt
     2701 atcagcaaag cttacatgca gaatcctaat gccatcatac tgtgtattca agatggatct
     2761 gtggatgctg aacgcagtat tgttacagac ttggtcagtc aaatggaccc tcatggaagg
     2821 agaaccatat tcgttttgac caaagtagac ctggcagaga aaaatgtagc cagtccaagc
     2881 aggattcagc agataattga aggaaagctc ttcccaatga aagctttagg ttattttgct
     2941 gttgtaacag gaaaagggaa cagctctgaa agcattgaag ctataagaga atatgaagaa
     3001 gagttttttc agaattcaaa gctcctaaag acaagcatgc taaaggcaca ccaagtgact
     3061 acaagaaatt taagccttgc agtatcagac tgcttttgga aaatggtacg agagtctgtt
     3121 gaacaacagg ctgatagttt caaagcaaca cgttttaacc ttgaaactga atggaagaat
     3181 aactatcctc gcctgcggga acttgaccgg aatgaactat ttgaaaaagc taaaaatgaa
     3241 atccttgatg aagttatcag tctgagccag gttacaccaa aacattggga ggaaatcctt
     3301 caacaatctt tgtgggaaag agtatcaact catgtgattg aaaacatcta ccttccagct
     3361 gcgcagacca tgaattcagg aacttttaac accacagtgg atatcaagct taaacagtgg
     3421 actgataaac aacttcctaa taaagcagta gaggttgctt gggagaccct acaagaagaa
     3481 ttttcccgct ttatgacaga accgaaaggg aaagagcatg atgacatatt tgataaactt
     3541 aaagaggccg ttaaggaaga aagtattaaa cgacacaagt ggaatgactt tgcggaggac
     3601 agcttgaggg ttattcaaca caatgctttg gaagaccgat ccatatctga taaacagcaa
     3661 tgggatgcag ctatttattt tatggaagag gctctgcagg ctcgtctcaa ggatactgaa
     3721 aatgcaattg aaaacatggt gggtccagac tggaaaaaga ggtggttata ctggaagaat
     3781 cggacccaag aacagtgtgt tcacaatgaa accaagaatg aattggagaa gatgttgaaa
     3841 tgtaatgagg agcacccagc ttatcttgca agtgatgaaa taaccacagt ccggaagaac
     3901 cttgaatccc gaggagtaga agtagatcca agcttgatta aggatacttg gcatcaagtt
     3961 tatagaagac attttttaaa aacagctcta aaccattgta acctttgtcg aagaggtttt
     4021 tattactacc aaaggcattt tgtagattct gagttggaat gcaatgatgt ggtcttgttt
     4081 tggcgtatac agcgcatgct tgctatcacc gcaaatactt taaggcaaca acttacaaat
     4141 actgaagtta ggcgattaga gaaaaatgtt aaagaggtat tggaagattt tgctgaagat
     4201 ggtgagaaga agattaaatt gcttactggt aaacgcgttc aactggcgga agacctcaag
     4261 aaagttagag aaattcaaga aaaacttgat gctttcattg aagctcttca tcaggagaaa
     4321 taagcggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     4381 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     4441 aaccattata agctgcaata aacaagttaa cgaattctac cgggtagggg aggcgctttt
     4501 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     4561 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     4621 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     4681 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     4741 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     4801 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     4861 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     4921 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     4981 gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     5041 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     5101 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     5161 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     5221 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     5281 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     5341 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     5401 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     5461 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     5521 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     5581 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     5641 gcacgacccc atgcatcgat aaaataaaag attttattta gtctccagaa aaagggggga
     5701 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca
     5761 tggaaaatac ataactgaga atagagaagt tcagatcaag gttaggaaca gagagacagc
     5821 agaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     5881 aacagatggt ccccagatgc ggtcccgccc tcagcagttt ctagagaacc atcagatgtt
     5941 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
     6001 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
     6061 cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg tgtatccaat
     6121 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct
     6181 gagtgattga ctacccgtca gcgggggtct ttcatgggta acagtttctt gaagttggag
     6241 aacaacattc tgagggtagg agtcgaatat taagtaatcc tgactcaatt agccactgtt
     6301 ttgaatccac atactccaat actcctgaaa tagttcatta tggacagcgc agaaagagct
     6361 ggggagaatt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6421 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6481 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6541 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6601 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6661 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6721 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6781 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6841 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6901 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6961 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7021 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7081 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7141 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7201 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7261 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7321 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7381 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7441 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7501 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7561 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7621 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7681 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7741 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7801 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7861 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7921 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7981 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8041 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8101 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8161 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8221 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8281 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8341 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8401 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8461 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     8521 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     8581 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     8641 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     8701 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     8761 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     8821 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     8881 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     8941 agtcacgacg ttgtaaaacg acggcgcaag gaatggtgca tgcaaggaga tggcgcccaa
     9001 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
     9061 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
     9121 acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggcgat tagtccaatt
     9181 tgttaaagac aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct
     9241 gaagcctata gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa
     9301 gggggg
//
