LOCUS       sequence-486510-        9156 bp    DNA     circular SYN 17-SEP-2025
DEFINITION  A CMV driven expression vector containing a Sun1-2xsfGFP for 
            labeling nuclear envenlope. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9156)
  AUTHORS   Gillespie W, Zhang Y, Ruiz OE, Cerda J, Ortiz-Guzman J, Turner WD, 
            Largoza G, Sherman M, Mosser LE, Fujimoto E, Chien CB, Kwan KM, 
            Arenkiel BR, Devine WP, Wythe JD
  TITLE     Multisite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox with diverse applications in vertebrate model 
            systems.
  JOURNAL   bioRxiv [Preprint]. 2024 Jul 13:2024.07.13.603267. doi: 
            10.1101/2024.07.13.603267.
  PUBMED    39026881
REFERENCE   2  (bases 1 to 9156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jul 13:2024.07.13.603267. doi: 
            10.1101/2024.07.13.603267."
COMMENT     Sequence Label: pCS2-Sun1-2xsfGFP-6xMYC-pA (JDW 694)
FEATURES             Location/Qualifiers
     source          1..9156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    108..132
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      214..223
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3040..3042,3043..3045,3046..3756)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3778..3780,3781..3783,3784..4494)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4534..4563
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4570..4599
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4606..4635
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4663..4692
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4699..4728
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4735..4764
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4787..4804)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4793..5017
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(5139..5163)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5208..5342
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5232..5251)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5286..5305
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5455..5475)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5455..5473)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5494..5510)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5494..5510)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5507..5529)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5518..5534
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5542..5548,5549..5566,5567..5572))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5587..5608
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5725..5742)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5896..6484)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5976..5995)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6655..7446,7447..7515))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7278..7297
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7516..7620)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7646..8101)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7783..7804)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7995..8014
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8172..9156
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca aggatcaaca agtttgtaca
      121 aaaaagcagg ctactagtgg ccgctagcgt ttgggcttaa ggggtctatc gatgggtctg
      181 cggccgcact actggccggc cactactacg cgtgccacca tggacttttc tcggctgcac
      241 acgtacaccc caccccagtg tgtgccggag aacactggct acacttacgc actcagttct
      301 agttactcgt cggatgctct ggattttgaa actgagcaca agttggaacc tgtatttgac
      361 tctccaagga tgtcccgccg cagcttgcgt ctggtcacaa cagcttcgta cagcagtggg
      421 gacagccagg ctattgattc gcacattagc accagcaggg ccacccccgc caaggggaga
      481 gaaaccagga cagtcaaaca gagaagaagt gcaagcaagc cagcttttag tatcaaccac
      541 ctgtcaggga agggcttgtc ctcgagcaca agccatgaca gctcttgcag cctgcggagt
      601 gccacggtgc tgcggcaccc tgtgctagat gagtccctga ttcgtgagca gaccaaagtg
      661 gaccacttct ggggtctcga tgatgatggt gaccttaaag gtggaaataa agctgccact
      721 cagggaaatg gtgaactggc agcagaggtg gcgagcagca atggatacac ttgccgtgac
      781 tgcaggatgc tctcagcgcg cactgacgca ctcacagccc actctgccat ccacgggacc
      841 acctccaggg tgtactccag agacaggact ctcaaaccac gcggagtgtc cttttacctg
      901 gataggactc tgtggctggc caagtccacc tcctcatcct ttgcatcatt tatagttcaa
      961 cttttccaag tggttttaat gaagctcaat tttgaaactt acaaattgaa aggctatgaa
     1021 tccagagctt atgaatcaca gagctatgag acaaagagcc atgagtcaga agcccatctc
     1081 ggtcactgtg ggaggatgac tgccggagaa ctttccagag tggacgggga gtccctgtgc
     1141 gatgactgta aggggaagaa gcaccttgag atacacacag ccacccactc gcaactgccc
     1201 cagccacaca gggtggccgg ggccatgggg cgcctctgca tctatacagg tgacctcttg
     1261 gttcaagcac tgcgaaggac tagagctgcc gggtggtctg tggccgaggc cgtgtggtcg
     1321 gtgctctggc tggctgtctc tgctccaggg aaggcagcct cgggaacctt ctggtggcta
     1381 gggagcggct ggtaccaatt tgttactttg atttcttggc tgaatgtctt tcttcttacc
     1441 aggtgccttc gaaatatttg caaggttttt gtcttgctcc tcccactcct acttttacta
     1501 ggtgctggtg tctccctgtg gggccaggga aacttcttct cactcctacc agtgctgaac
     1561 tggacggcca tgcagccaac acagagggtg gacgattcca agggcatgca tagacctggc
     1621 cctcttcccc cgagcccacc tccaaaggtt gatcacaagg cttcccagtg gcctcaggag
     1681 agtgacatgg ggcagaaggt agcttctttg agtgcgcagt gccacaacca tgatgagaga
     1741 cttgcagagc tgacagtcct gcttcagaaa ctacagatac gggtagacca agtggatgac
     1801 ggcagggaag ggctgtcact gtgggtcaag aatgtggttg gacagcacct gcaggagatg
     1861 ggcaccatag aaccacctga tgctaagact gacttcatga ctttccacca tgaccatgaa
     1921 gtgcgtctct ccaacttgga agatgttctt agaaaactga cagaaaaatc tgaggctatc
     1981 cagaaggagc tggaagaaac caagctgaaa gcaggcagca gggatgaaga gcagcccctc
     2041 cttgaccgtg tgcagcacct agaactggaa ctgaacctgt tgaagtcaca gctgtcagac
     2101 tggcagcatc tgaagaccag ctgtgagcag gctggggccc gcatccagga gactgtgcag
     2161 ctcatgttct ctgaggatca gcagggcggt tccctcgagt ggctattaga gaagctttct
     2221 tctcggttcg tgagcaagga tgagctgcag gtgctcttac atgaccttga gctgaaactg
     2281 ctgcagaata tcacacacca catcaccgtg acaggacagg ccccgacatc cgaggctatt
     2341 gtgtctgccg tgaatcaggc agggatttca ggaatcacag aagcgcaagc acatatcatt
     2401 gtgaacaatg ctctgaagct gtactcccaa gacaagacgg ggatggtgga ctttgctctg
     2461 gagtctggag gtggcagcat cctaagcact cggtgctctg agacctatga gaccaagacg
     2521 gcactgctga gcctgtttgg ggtcccactg tggtacttct cacagtcacc tcgagtggtg
     2581 atccagcccg acatctaccc agggaattgc tgggcgttca aaggttccca ggggtacctg
     2641 gtggtgcggt tgtccatgaa gatctaccca accacattca ccatggaaca cattccaaag
     2701 acactatcac ccactggtaa catctccagt gcccccaaag actttgcagt ctatggactg
     2761 gaaacggagt atcaagaaga ggggcagcct ctgggacggt tcacctatga ccaggaagga
     2821 gactcactcc agatgttcca cacactggaa agacctgacc aagccttcca gatagtagag
     2881 ctccgggtcc tgtccaactg gggccaccct gagtacactt gcctctaccg gttccgagtc
     2941 cacggagagc ccatccaggg aggcgtggcg cgcctcgccg cagcctccgg cggcggaggc
     3001 tctggcggag ggggctccct cgcagcagcc tctgaattca tggtgagcaa gggcgaggag
     3061 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     3121 ttcagcgtgc gcggcgaggg cgagggcgat gccaccaacg gcaagctgac cctgaagttc
     3181 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     3241 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     3301 gccatgcccg aaggctacgt ccaggagcgc accatcagct tcaaggacga cggcacctac
     3361 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     3421 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactttaac
     3481 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
     3541 atccgccaca acgtggagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     3601 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgtg
     3661 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     3721 gccgggatca ctctcggcat ggacgagctg tacaagggag gcggaggtgc aagatctatg
     3781 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     3841 gacgtaaacg gccacaagtt cagcgtgcgc ggcgagggcg agggcgatgc caccaacggc
     3901 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     3961 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     4021 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcagcttc
     4081 aaggacgacg gcacctacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     4141 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     4201 ctggagtaca actttaacag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
     4261 atcaaggcca acttcaagat ccgccacaac gtggaggacg gcagcgtgca gctcgccgac
     4321 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     4381 ctgagcaccc agtccgtgct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     4441 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagagatct
     4501 gaattcggcg gaggatcccc cgggggccgc tctgagcaaa agctcatttc tgaagaggac
     4561 ttgaatggag aacagaaatt gatcagtgag gaagacctca acggtgagca gaagttaata
     4621 tccgaggagg atcttaatag tagcggcagt ggtagccgct ctgagcaaaa gctcatttct
     4681 gaagaggact tgaatggaga acagaaattg atcagtgagg aagacctcaa cggtgagcag
     4741 aagttaatat ccgaggagga tcttaatagt agcggttaat aagcttcctc gactgtgcct
     4801 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     4861 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     4921 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     4981 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgagcataa cgcgcttaat
     5041 taaggggatc cgggatatcg ggtctattta aattctgggg gcctttaagg ccgcgcgcgt
     5101 ctagagtctg gtaccgggcc tagggggctc gaggggtctc ccagctttct tgtacaaagt
     5161 ggttgatcct ctcgagcctc tagaactata gtgagtcgta ttacgtagat ccagacatga
     5221 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     5281 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     5341 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     5401 tttaattcgc ggccgcggcg ccaatgcatt gggcccggta cccagctttt gttcccttta
     5461 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     5521 ttatccgctc acaattccac acaacatacg agccgggagc ataaagtgta aagcctgggg
     5581 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     5641 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     5701 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     5761 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     5821 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     5881 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     5941 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6001 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6061 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6121 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6181 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6241 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6301 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     6361 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6421 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6481 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     6541 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     6601 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     6661 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     6721 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     6781 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     6841 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     6901 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     6961 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7021 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7081 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7141 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7201 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7261 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7321 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7381 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     7441 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     7501 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     7561 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     7621 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
     7681 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     7741 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     7801 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     7861 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     7921 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     7981 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     8041 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     8101 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     8161 ctattacgcc agtcgaccgc caattcaata tggcgtatat ggactcatgc caattcaata
     8221 tggtggatct ggacctgtgc caattcaata tggcgtatat ggactcgtgc caattcaata
     8281 tggtggatct ggaccccagc caattcaata tggcggactt ggcaccatgc caattcaata
     8341 tggcggacct ggcactgtgc caactgggga ggggtctact tggcacggtg ccaagtttga
     8401 ggaggggtct tggccctgtg ccaagtccgc catattgaat tggcatggtg ccaataatgg
     8461 cggccatatt ggctatatgc caggatcaat atataggcaa tatccaatat ggccctatgc
     8521 caatatggct attggccagg ttcaatacta tgtattggcc ctatgccata tagtattcca
     8581 tatatgggtt ttcctattga cgtagatagc ccctcccaat gggcggtccc atataccata
     8641 tatggggctt cctaataccg cccatagcca ctcccccatt gacgtcaatg gtctctatat
     8701 atggtctttc ctattgacgt catatgggcg gtcctattga cgtatatggc gcctccccca
     8761 ttgacgtcaa ttacggtaaa tggcccgcct ggctcaatgc ccattgacgt caataggacc
     8821 acccaccatt gacgtcaatg ggatggctca ttgcccattc atatccgttc tcacgccccc
     8881 tattgacgtc aatgacggta aatggcccac ttggcagtac atcaatatct attaatagta
     8941 acttggcaag tacattacta ttggaagtac gccagggtac attggcagta ctcccattga
     9001 cgtcaatggc ggtaaatggc ccgcgatggc tgccaagtac atccccattg acgtcaatgg
     9061 ggaggggcaa tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa
     9121 tgggaggtct atataagcaa tgctcgttta gggaac
//
