LOCUS       sequence-486524-        8629 bp    DNA     circular SYN 17-SEP-2025
DEFINITION  pUltra-EGFP bi-cistronic lentiviral vector expressing EGFP and 
            truncated NRL..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Shayler DWH, Stachelek K, Cambier L, Lee S, Bai J, Reid MW, 
            Weisenberger DJ, Bhat B, Aparicio JG, Kim Y, Singh M, Bay M, 
            Thornton ME, Doyle EK, Fouladian Z, Erberich SG, Grubbs BH, 
            Bonaguidi MA, Craft CM, Singh HP, Cobrinik D
  TITLE     Identification and characterization of early human photoreceptor 
            states and cell-state-specific retinoblastoma-related features
  JOURNAL   eLife 13:RP101918
REFERENCE   2  (bases 1 to 8629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife 
            13:RP101918"
COMMENT     Sequence Label: pUltra-EGFP-P2A-Tr-NRL
FEATURES             Location/Qualifiers
     source          1..8629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1840..1957
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2022..3233
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3070..3089
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3266..3275
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3272..3274,3275..3277,3278..3988)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3272..3274,3275..3277,3278..3988)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3317..3338)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3578..3597)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3925..3946
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3998..4054
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4379..4432
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    4476..5064
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4529..4549)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4947..4958)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5155..5388
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5460..5581
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5497..5516)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5551..5570
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5621..5756
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5776..5795)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5777..5795)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5805..5822)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5805..5821)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5814..5836)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5963..6418
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6050..6069)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6260..6281
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6444..6548
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6549..6617,6618..7409)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6767..6786)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7580..8168
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8069..8088
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8322..8339
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8456..8477
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8492..8497,8498..8515,8516..8522)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8530..8546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8535..8557
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8554..8570
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8554..8570
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8589..8609
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8591..8609
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     1801 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     1861 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     1921 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     1981 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt
     2041 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
     2101 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
     2161 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
     2221 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
     2281 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
     2341 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
     2401 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
     2461 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
     2521 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
     2581 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
     2641 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     2701 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     2761 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
     2821 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
     2881 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     2941 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     3001 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     3061 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     3121 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     3181 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
     3241 gggctgcagg tccgatccac cggtcgccac catggtgagc aagggcgagg agctgttcac
     3301 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     3361 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     3421 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     3481 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     3541 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     3601 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     3661 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     3721 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     3781 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     3841 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     3901 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     3961 cactctcggc atggacgagc tgtacaaggg atctggagca acaaacttct cactactcaa
     4021 acaagcaggt gacgtggagg agaatcccgg gccttctaga ggatccatgt ctgtgcggga
     4081 gctaaaccgg cagctgcggg gctgcgggcg cgacgaggcg ctgcggctga agcagaggcg
     4141 ccgcacgctg aagaaccgcg gctacgcgca ggcctgtcgc tccaagcggc tgcagcagcg
     4201 gcgcgggctg gaggccgagc gcgcccgcct ggccgcccag ctggacgcgc tgcgggccga
     4261 ggtggcccgc ctggcccggg agcgcgatct ctacaaggct cgctgtgacc ggctaacctc
     4321 gagcggcccc gggtccgggg acccctccca cctcttcctc tgaggatccg gctccggcga
     4381 gggcagggga agtctactaa catgcgggga cgtggaggaa aatcccggcc cagctagcgt
     4441 gtgatcagaa ttcgttccgg agtcgtcgac tcgacaatca acctctggat tacaaaattt
     4501 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4561 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4621 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4681 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4741 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4801 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4861 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     4921 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4981 gcctgctgcc ggctctgcgg cctcttccgc atcttcgcct tcgccctcag acgagtcgga
     5041 tctccctttg ggccgcctcc ccgcctggaa ttaattcgag ctcggtacct ttaagaccaa
     5101 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     5161 ggctacgtaa ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     5221 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5281 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5341 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     5401 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     5461 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5521 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5581 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     5641 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5701 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5761 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     5821 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     5881 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     5941 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6001 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6061 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6121 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6181 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6241 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6301 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6361 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     6421 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6481 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6541 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6601 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6661 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6721 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6781 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6841 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6901 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6961 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7021 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7081 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7141 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7201 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7261 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7321 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7381 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7441 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7501 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7561 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7621 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7681 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7741 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7801 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7861 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7921 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7981 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8041 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8101 ggtttcgcca cctctcactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8161 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8221 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8281 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8341 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8401 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     8461 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     8521 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     8581 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgca
//
