LOCUS       sequence-486527-        8681 bp    DNA     circular SYN 17-SEP-2025
DEFINITION  Mammalian expression of wild-type LbCas12a with 2xSV40 NLS at 
            N-terminus and 2xSV40 at C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Ma E, Chen K, Shi H, Wasko KM, Esain-Garcia I, Trinidad MI, Zhou K, 
            Ye J, Doudna JA
  TITLE     Directed evolution expands CRISPR-Cas12a genome-editing capacity.
  JOURNAL   Nucleic Acids Res. 2025 Jul 8;53(13):gkaf649. doi: 
            10.1093/nar/gkaf649.
  PUBMED    40671523
REFERENCE   2  (bases 1 to 8681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-07-8"; volume: "53(13):gkaf649. doi"; 
            pages: " 10.1093/nar/gkaf649"
COMMENT     Sequence Label: pEM1_Euk_2NLS-WT ( LbCas12a)-2NLS
FEATURES             Location/Qualifiers
     source          1..8681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1167..1546
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1548..1823
                     /label=chicken beta-actin promoter
     intron          1824..2840
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2764..2786
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2848..2867
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2933..2942
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2945..2965
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2975..2995
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3008..6688
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             6689..6736
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6755..6775
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6865..6884)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6930..6985
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6984..7003)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7346..7362)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7346..7362)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7359..7381)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7370..7386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7394..7400,7401..7418,7419..7424))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7439..7460
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7519..7714
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7565..7700
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7720..7854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7757..7776)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7811..7830
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7922..7939)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8093..8681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8173..8192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgggtcgaca ttgattattg actagttatt aatagtaatc
     1201 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1261 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1321 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1381 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     1441 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     1501 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
     1561 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt
     1621 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc gcgccaggcg
     1681 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
     1741 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
     1801 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
     1861 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
     1921 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
     1981 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg
     2041 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg
     2101 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg
     2161 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga
     2221 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg
     2281 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
     2341 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
     2401 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc
     2461 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
     2521 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
     2581 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
     2641 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
     2701 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
     2761 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
     2821 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     2881 ttggcaaaga attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat
     2941 gggcccaaag aaaaagagga aagtcggcag tggacctaaa aagaaacgaa aggttgggtc
     3001 aggttctagc aagctggaaa aatttaccaa ctgttacagc ctgagcaaaa ccctgaggtt
     3061 caaggccatc cccgtgggca agacccaaga gaacatcgat aacaagagac tgctggtcga
     3121 ggatgagaag cgtgcagagg actacaaggg cgtgaagaag ctcctggatc ggtactacct
     3181 gagtttcatc aacgacgtgc tgcacagcat caagctgaag aacctgaaca actacatcag
     3241 cctgtttaga aagaaaacca gaaccgaaaa agaaaataag gagctggaaa atctggaaat
     3301 caacctgaga aaggaaatcg ctaaggcctt taagggaaac gaaggataca aatccctgtt
     3361 taagaaagac atcatcgaga caattctgcc agagttcctg gacgacaaag acgagatagc
     3421 tcttgtgaac agcttcaacg gcttcacgac cgccttcacc ggattcttcg acaacaggga
     3481 aaacatgttc agtgaggaag ccaagtctac cagcatcgct ttccggtgca tcaatgagaa
     3541 cctgacccgg tatatctcta atatggatat ctttgaaaaa gtggacgcta ttttcgacaa
     3601 gcacgaggtg caggagatca aggagaagat cctgaacagc gactacgacg tggaggactt
     3661 cttcgagggc gaatttttca acttcgtgct gacacaagag ggcatcgacg tttacaacgc
     3721 catcataggc ggcttcgtga ccgagtccgg cgaaaagatc aagggcctta acgagtacat
     3781 caacctgtat aaccagaaaa ccaagcagaa gctgcctaag ttcaagcctc tttataagca
     3841 ggtcctgagc gaccgggaaa gcctgagctt ctacggcgag ggctacacat ctgacgagga
     3901 agtgctggag gtctttcgga ataccctgaa caagaactct gagatcttca gctctattaa
     3961 gaaactggaa aagctgttca agaatttcga cgagtatagt agcgccggga tcttcgtgaa
     4021 gaacggccct gccattagca ccatcagcaa ggacatcttc ggcgaatgga acgttatccg
     4081 cgataagtgg aacgccgagt acgatgacat ccacctgaaa aagaaggccg tggtgaccga
     4141 gaagtacgaa gatgacagaa gaaagagctt caaaaagatc ggctcattta gcctggaaca
     4201 gctgcaggag tacgccgacg ccgacctgtc cgtggtcgaa aagctgaagg aaattatcat
     4261 ccaaaaagtg gacgagatct ataaggtgta cgggagctct gagaagctgt tcgacgccga
     4321 ctttgtgctg gaaaagtcac tgaaaaagaa cgacgctgtg gtcgccatca tgaaggatct
     4381 gctggactcc gtgaagagct tcgagaacta catcaaggcc ttcttcggcg aaggcaagga
     4441 gaccaaccgg gacgagtctt tttacggcga tttcgtgctg gcctacgaca tcctgctgaa
     4501 ggtggaccac atctacgacg ccatcagaaa ctacgtgacc cagaagccct acagcaaaga
     4561 caagtttaag ctgtacttcc agaatcctca gttcatggga ggatgggaca aagacaagga
     4621 aaccgactac cgggctacaa ttctgagata cggctctaag tactaccttg caatcatgga
     4681 caaaaagtat gccaagtgcc tgcagaaaat tgataaggat gacgtgaacg gcaactacga
     4741 gaagatcaac tacaagctcc tgcccggccc caacaagatg ctgccaaagg tgttcttctc
     4801 caaaaagtgg atggcctatt acaacccttc tgaggatatc cagaagatct ataagaacgg
     4861 aacattcaag aagggcgata tgttcaacct gaatgactgc cacaagctga tcgacttctt
     4921 taaagacagc atctccagat accctaagtg gtccaacgcc tacgacttca acttcagcga
     4981 aacagaaaag tacaaggaca tcgccggctt ctacagagag gtggaagaac aaggctacaa
     5041 ggtcagcttc gagagcgcta gcaagaagga ggtggacaag ctggtggagg agggaaagct
     5101 gtacatgttc cagatctaca acaaggattt cagcgacaag agccacggca cacctaacct
     5161 gcacaccatg tatttcaagc tgctgtttga cgagaacaac catggccaga tccggctgtc
     5221 tggcggcgcc gaactgttca tgcggagagc ctctttgaag aaggaagagc tggtggtgca
     5281 ccctgccaat agccctatcg ccaacaagaa cccagataac ccaaagaaaa ccaccacact
     5341 gagctacgac gtgtacaaag ataagagatt cagcgaggat cagtacgagc tgcatatccc
     5401 tatcgccatc aataagtgtc ctaaaaacat cttcaagatc aataccgagg tgcgggtgtt
     5461 gctgaagcac gacgataacc cttacgtgat cggaatcgac aggggcgaga gaaacctgct
     5521 gtacatcgtg gtggtggacg gcaagggcaa tatcgtggag caatacagcc tgaatgagat
     5581 catcaacaat ttcaacggca tccgcataaa aacagattac cactctctgc tggataagaa
     5641 agagaaagag cggtttgagg ccagacagaa ctggaccagc atcgagaata tcaaagagtt
     5701 aaaggccggc tacatcagcc aggtggtgca caaaatctgc gagctggtgg aaaagtacga
     5761 cgctgtgatc gcccttgagg atctaaacag cggctttaag aacagcagag tgaaggtaga
     5821 aaagcaggtg tatcagaagt tcgagaagat gctcatcgat aagctgaact acatggtgga
     5881 taagaaaagc aacccctgcg ccacaggcgg agctctgaag ggctaccaga tcaccaacaa
     5941 attcgagagc tttaagagca tgtcgaccca gaatggtttc atcttttaca tccccgcctg
     6001 gctcacctca aagatcgatc ccagcacagg cttcgtcaac ctgctgaaaa ccaagtacac
     6061 cagcatcgcc gatagcaaga agtttatctc gtccttcgac agaatcatgt acgtgcccga
     6121 ggaggacctg ttcgagttcg ccctggacta caagaacttc tcccggacag atgccgacta
     6181 cattaagaag tggaagctgt acagctacgg aaacaggatc agaatcttta gaaaccccaa
     6241 aaagaacaac gtattcgatt gggaggaagt gtgcctgacc tcggcctaca aggagctgtt
     6301 caacaagtac ggcatcaact accagcaggg cgacatccgg gccctgctct gtgaacagag
     6361 cgacaaggct ttctacagca gcttcatggc cctgatgagc ctgatgctgc agatgagaaa
     6421 cagcatcaca ggaagaacag acgtggactt tctgatctct cctgtgaaga acagcgacgg
     6481 catcttctac gacagcagaa actatgaagc ccaggagaac gcgatcctgc ctaagaacgc
     6541 agacgccaac ggcgcctaca atatcgccag aaaggtgctg tgggccattg gccagttcaa
     6601 gaaggctgaa gatgagaaac tggataaagt gaagatcgcc atctccaaca aggaatggct
     6661 ggaatacgcc cagaccagcg tgaagcacaa aaggccggcg gccacgaaaa aggccggcca
     6721 ggcaaaaaag aaaaagggat cctctggtgg ttctcccaag aagaagagga aagtctaaag
     6781 cggccgcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     6841 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     6901 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     6961 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     7021 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     7081 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     7141 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     7201 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     7261 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     7321 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7381 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7441 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7501 tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     7561 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7621 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     7681 aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat
     7741 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7801 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc
     7861 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7921 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7981 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8041 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8101 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8161 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8221 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8281 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8341 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8401 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8461 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8521 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8581 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8641 tttgcaagca gcagattacg cgcagaaaaa aaggatctca a
//
