LOCUS       sequence-486580-        9739 bp    DNA     circular SYN 17-SEP-2025
DEFINITION  Mammalian expression of Flex-Cas12a cytosine base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Ma E, Chen K, Shi H, Wasko KM, Esain-Garcia I, Trinidad MI, Zhou K, 
            Ye J, Doudna JA
  TITLE     Directed evolution expands CRISPR-Cas12a genome-editing capacity.
  JOURNAL   Nucleic Acids Res. 2025 Jul 8;53(13):gkaf649. doi: 
            10.1093/nar/gkaf649.
  PUBMED    40671523
REFERENCE   2  (bases 1 to 9739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-07-8"; volume: "53(13):gkaf649. doi"; 
            pages: " 10.1093/nar/gkaf649"
COMMENT     Sequence Label: pHS638_Flex-Cas12a-CBE
FEATURES             Location/Qualifiers
     source          1..9739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          163..1179
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1103..1125
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1187..1206
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1272..1281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1284..1304
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1314..1334
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1344..2027
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             5805..5852
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5889..6137
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6150..6170
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6260..6279)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6325..6380
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6379..6398)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6741..6757)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6741..6757)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6754..6776)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6765..6781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6789..6795,6796..6813,6814..6819))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6834..6855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6914..7109
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6960..7095
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7115..7249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7152..7171)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7206..7225
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7317..7334)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7488..8076)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7568..7587)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8247..9038,9039..9107))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8870..8889
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9108..9212)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        9243..9622
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggggggcgc gccrgggggg gggggggggg gggggggggg gggggggggg ggggsggrgr
       61 ggkgsggsgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
      121 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc
      181 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
      241 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
      301 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg
      361 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
      421 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg
      481 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
      541 ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca
      601 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct
      661 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg
      721 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg
      781 gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg
      841 agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc
      901 caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
      961 gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg
     1021 cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt
     1081 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     1141 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     1201 attgtgctgt ctcatcattt tggcaaagaa ttctgcagtc gacggtaccg cgggcccggg
     1261 atccaccggt cgccaccatg ggcccaaaga aaaagaggaa agtcggcagt ggacctaaaa
     1321 agaaacgaaa ggttgggtca ggtagctcag agactggccc agtggctgtg gaccccacat
     1381 tgagacggcg gatcgagccc catgagtttg aggtattctt cgatccgaga gagctccgca
     1441 aggagacctg cctgctttac gaaattaatt gggggggccg gcactccatt tggcgacata
     1501 catcacagaa cactaacaag cacgtcgaag tcaacttcat cgagaagttc acgacagaaa
     1561 gatatttctg tccgaacaca aggtgcagca ttacctggtt tctcagctgg agcccatgcg
     1621 gcgaatgtag tagggccatc actgaattcc tgtcaaggta tccccacgtc actctgttta
     1681 tttacatcgc aaggctgtac caccacgctg acccccgcaa tcgacaaggc ctgcgggatt
     1741 tgatctcttc aggtgtgact atccaaatta tgactgagca ggagtcagga tactgctgga
     1801 gaaactttgt gaattatagc ccgagtaatg aagcccactg gcctaggtat ccccatctgt
     1861 gggtacgact gtacgttctt gaactgtact gcatcatact gggcctgcct ccttgtctca
     1921 acattctgag aaggaagcag ccacagctga cattctttac catcgctctt cagtcttgtc
     1981 attaccagcg actgccccca cacattctct gggccaccgg gttgaaatct ggtggttctt
     2041 ctggtggttc tagcggcagc gagactcccg ggacctcaga gtccgccaca cccgaaagtt
     2101 ccggagggag tagcggcggg tctagcaagc tggaaaaatt taccaactgt tacagcctga
     2161 gcaaaaccct gaggttcaag gccatccccg tgggcaagac ccaagagaac atcgataaca
     2221 agagactgct ggtcgaggat gagaagcgtg cagaggacta caagggcgtg aagaagctcc
     2281 tggatcggta ctacctgagt ttcatcaacg acgtgctgca cagcatcaag ctgaagaacc
     2341 tgaacaacta catcagcctg tttagaaaga aaaccagaac cgaaaaagaa aataaggagc
     2401 tggaaaatct ggaaatcaac ctgagaaagg aaatcgctaa ggcctttaag ggaaacgaag
     2461 gatacaaatc cctgtttaag aaagacatca tcgagacaat tctgccagag ttcctggacg
     2521 acaaagacga gatagctctt gtgaacagct tcaacaggtt cacgaccgcc ttcaccggat
     2581 tcttcgacaa cagggaaaac atgttcagtg aggaagccaa gtctaccagc atcgctttcc
     2641 ggtgcatcaa tgagaacctg accgtgtata tctctaatat ggatatcttt gaaaaagtgg
     2701 acgctatttt cgacaagcac gaggtgcagg agatcaagga gaagatcctg aacagcgact
     2761 acgacgtgga ggacttcttc gagggcgaat ttttcaactt cgtgctgaca caagagggca
     2821 tcgacgttta caacgccatc ataggcggct tcgtgaccga gtccggcgaa aagatcaagg
     2881 gccttaacga gtacatcaac ctgtataacc agaaaaccaa gcagaagctg cctaagttca
     2941 agcctcttta taagcaggtc ctgagcgacc gggaaagcct gagcttctac ggcgagggct
     3001 acacatctga cgaggaagtg ctggaggtct ttcggaatac cctgaacaag aactctgaga
     3061 tcttcagctc tattaagaaa ctggaaaagc tgttcaagaa tttcgacgag tatagtagcg
     3121 ccgggatctt cgtgaagaac ggccctgcca ttagcaccat cagcaaggac atcttcggcg
     3181 aatggaacgt tatccgcgat aagtggaacg ccgagtacga tgacatccac ctgaaaaaga
     3241 aggccgtggt gaccgagaag tacgaagatg acagaagaaa gagcttcaaa aagatcggct
     3301 catttagcct ggaacagctg caggagtacg ccgacgccga cctgtccgtg gtcgaaaagc
     3361 tgaaggaaat tatcatccaa aaagtggacg agatctataa ggtgtacggg agctctgaga
     3421 agctgttcga cgccgacttt gtgctggaaa agtcactgaa aaagaacgac gctgtggtcg
     3481 ccatcatgaa ggatctgctg gactccgtga agagcttcga gaactacatc aaggccttct
     3541 tcggcgaagg caaggagacc aaccgggacg agtcttttta cggcgatttc gtgctggcct
     3601 acgacatcct gctgaaggtg gaccacatct acgacgccat cagaaactac gtgacccaga
     3661 agccctacag caaagacaag tttaagctgt acttccagaa tcctcagttc atgggaggat
     3721 ggggcaaaga caaggaaacc gactaccggg ctacaattct gagatacggc tttaagtact
     3781 accttgcaat catggacaaa aagtatgcca agtgcctgca gaaaattgat aaggatgacg
     3841 tgaacggcaa ctacgagaag atcaactaca agctcctgcc cggccccaac aagatgctgc
     3901 caaaggtgtt cttctccaaa aagtggatgg cctattacaa cccttctgag gatatccaga
     3961 agatctataa gaacggaaca ttcaagaagg gcgatatgtt caacctgaat gactgccaca
     4021 agctgatcga cttctttaaa gacagcatct ccagataccc taagtggtcc aacgcctacg
     4081 acttcaactt cagcgaaaca gaaaagtaca agaacatcgc cggcttctac agagaggtgg
     4141 aagaacaagg ctacaaggtc agcttcgaga gcgctagcaa gaaggaggtg gacaagctgg
     4201 tggaggaggg aaagctgtac atgttccaga tctacaacaa ggatttcagc gacaagagcc
     4261 acggcacacc taacctgcac accatgtatt tcaagctgct gtttgacgag aacaaccatg
     4321 gccagatccg gctgtctggc ggcgccgaac tgttcatgcg gagagcctct ttgaagaagg
     4381 aagagctggt ggtgcaccct gccaatagcc ctatcgccaa caagaaccca gataacccaa
     4441 agaaaaccac cacactgagc tacgacgtgt acaaagataa gagattcagc gaggatcagt
     4501 accagctgca tatccctatc gccatcaata agtgtcctaa aaacatcttc aagatcaata
     4561 ccgaggtgcg ggtgttgctg aagcacgacg ataaccctta cgtgatcgga atcgccaggg
     4621 gcgagagaaa cctgctgtac atcgtggtgg tggacggcaa gggcaatatc gtggagcaat
     4681 acagcctgaa tgagatcatc aacaatttca acggcatccg cataaaaaca gattaccact
     4741 ctctgctgga taagaaagag aaagagcggt ttgaggccag acagaactgg accagcatcg
     4801 agaatatcaa agagttaaag gccggctaca tcagccaggt ggtgcacaaa atctgcgagc
     4861 tggtggaaaa gtacgacgct gtgatcgccc ttgaggatct aaacagcggc tttaagaaca
     4921 gcagagtgaa ggtagaaaag caggtgtatc agaagttcga gaagatgctc atcgataagc
     4981 tgaactacat ggtggataag aaaagcaacc cctgcgccac aggcggagct ctgaagggct
     5041 accagatcac caacaaattc gagagcttta agagcatgtc gacccagaat ggtttcatct
     5101 tttacatccc cgcctggctc acctcaaaga tcgatcccag cacaggcttc gtcaacctgc
     5161 tgaaaaccaa gtacaccagc atcgccgata gcaagaagtt tatctcgtcc ttcgacagaa
     5221 tcatgtacgt gcccgaggag gacctgttcg agttcgccct ggactacaag aacttctccc
     5281 ggacagatgc cgactacatt aagaagtgga agctgtacag ctacggaaac aggatcagaa
     5341 tctttagaaa ccccaaaaag aacaacgtat tcgattggga ggaagtgtgc ctgacctcgg
     5401 cctacaagga gctgttcaac aagtacggca tcaactacca gcagggcgac atccgggccc
     5461 tgctctgtga acagagcgac aaggctttct acagcagctt catggccctg atgagcctga
     5521 tgctgcagat gagaaacagc atcacaggaa gaacagacgt ggactttctg atctctcctg
     5581 tgaagaacag cgacggcatc ttctacgaca gcagaaacta tgaagcccag gagaacgcga
     5641 tcctgcctaa gaacgcagac gccaacggcg cctacaatat cgccagaaag gtgctgtggg
     5701 ccattggcca gttcaagaag gctgaagatg agaaactgga taaagtgaag atcgccatct
     5761 ccaacaagga atggctggaa tacgcccaga ccagcgtgaa gcacaaaagg ccggcggcca
     5821 cgaaaaaggc cggccaggca aaaaagaaaa agggatcctc tggtggttct ggaggatctg
     5881 gtggttctac taatctgtca gatattattg aaaaggagac cggtaagcaa ctggttatcc
     5941 aggaatccat cctcatgctc ccagaggagg tggaagaagt cattgggaac aagccggaaa
     6001 gcgatatact cgtgcacacc gcctacgacg agagcaccga cgagaatgtc atgcttctga
     6061 ctagcgacgc ccctgaatac aagccttggg ctctggtcat acaggatagc aacggtgaga
     6121 acaagattaa gatgctctct ggtggttctc ccaagaagaa gaggaaagtc taaagcggcc
     6181 gcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg
     6241 ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     6301 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     6361 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     6421 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
     6481 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
     6541 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
     6601 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
     6661 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc
     6721 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6781 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6841 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6901 tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     6961 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7021 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     7081 gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg
     7141 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7201 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa
     7261 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7321 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7381 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7441 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7501 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7561 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7621 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7681 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7741 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7801 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7861 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7921 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7981 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8041 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8101 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8161 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8221 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8281 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8341 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8401 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8461 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8521 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8581 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8641 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8701 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8761 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8821 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8881 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8941 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9001 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9061 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9121 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9181 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg
     9241 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     9301 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     9361 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     9421 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     9481 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     9541 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     9601 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
     9661 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
     9721 gatgggggcg ggggggggg
//
