LOCUS       sequence-486613-       12360 bp    DNA     circular SYN 17-SEP-2025
DEFINITION  for chromosome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12360)
  AUTHORS   Sun C, Li H, Liu Y, Li Y, Gao R, Shi X, Fei H, Liu J, Liang R, Gao C
  TITLE     Iterative recombinase technologies for efficient and precise genome 
            engineering across kilobase to megabase scales.
  JOURNAL   Cell. 2025 Aug 1:S0092-8674(25)00800-1. doi: 
            10.1016/j.cell.2025.07.011.
  PUBMED    40763736
REFERENCE   2  (bases 1 to 12360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 1:S0092-8674(25)00800-1. doi: 10.1016/j.cell.2025.07.011."
COMMENT     Sequence Label: PCE(cm24Cre)
FEATURES             Location/Qualifiers
     source          1..12360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2009..2035
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             2075..2095
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     RBS             6109..6117
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6419..6439
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8165..8185
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9311..9337
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             9377..9397
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9460..9636
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(10190..10206)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10190..10206)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10203..10225)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10214..10230
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10238..10244,10245..10262,10263..10268))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10283..10304
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10421..10438)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10592..11180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10672..10691)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11351..12142,12143..12211))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11974..11993
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12212..12316)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccccctctct
     1021 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
     1081 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
     1141 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
     1201 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
     1261 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
     1321 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
     1381 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
     1441 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     1501 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     1561 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     1621 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     1681 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     1741 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     1801 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     1861 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     1921 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     1981 gtttggtgtt acttctgcaa agcttatgcc tgccgccaag agagtcaagc tcgatggcgg
     2041 caagaggacc gccgatggca gcgagttcga gtctcctaag aagaagagga aggtcgacaa
     2101 gaagtactcg atcggcctcg atattgggac taactctgtt ggctgggccg tgatcaccga
     2161 cgagtacaag gtgccctcaa agaagttcaa ggtcctgggc aacaccgatc ggcattccat
     2221 caagaagaat ctcattggcg ctctcctgtt cgacagcggc gagacggctg aggctacgcg
     2281 gctcaagcgc accgcccgca ggcggtacac gcgcaggaag aatcgcatct gctacctgca
     2341 ggagattttc tccaacgaga tggcgaaggt tgacgattct ttcttccaca ggctggagga
     2401 gtcattcctc gtggaggagg ataagaagca cgagcggcat ccaatcttcg gcaacattgt
     2461 cgacgaggtt gcctaccacg agaagtaccc tacgatctac catctgcgga agaagctcgt
     2521 ggactccaca gataaggcgg acctccgcct gatctacctc gctctggccc acatgattaa
     2581 gttcaggggc catttcctga tcgaggggga tctcaacccg gacaatagcg atgttgacaa
     2641 gctgttcatc cagctcgtgc agacgtacaa ccagctcttc gaggagaacc ccattaatgc
     2701 gtcaggcgtc gacgcgaagg ctatcctgtc cgctaggctc tcgaagtctc ggaagctcga
     2761 gaacctgatc gcccagctgc cgggcgagaa gaagaacggc ctgttcggga atctcattgc
     2821 gctcagcctg gggctcacgc ccaacttcaa gtcgaatttc gatctcgctg aggacgccaa
     2881 gctgcagctc tccaaggaca catacgacga tgacctggat aacctcctgg cccagatcgg
     2941 cgatcagtac gcggacctgt tcctcgctgc caagaatctg tcggacgcca tcctcctgtc
     3001 tgatattctc agggtgaaca ccgagattac gaaggctccg ctctcagcct ccatgatcaa
     3061 gcgctacgac gagcaccatc aggatctgac cctcctgaag gcgctggtca ggcagcagct
     3121 ccccgagaag tacaaggaga tcttcttcga tcagtcgaag aacggctacg ctgggtacat
     3181 tgacggcggg gcctctcagg aggagttcta caagttcatc aagccgattc tggagaagat
     3241 ggacggcacg gaggagctgc tggtgaagct caagcgcgag gacctcctga ggaagcagcg
     3301 gacattcgat aacggcagca tcccacacca gattcatctc ggggagctgc acgctatcct
     3361 gaggaggcag gaggacttct accctttcct caaggataac cgcgagaaga tcgagaagat
     3421 tctgactttc aggatcccgt actacgtcgg cccactcgct aggggcaact cccgcttcgc
     3481 ttggatgacc cgcaagtcag aggagacgat cacgccgtgg aacttcgagg aggtggtcga
     3541 caagggcgct agcgctcagt cgttcatcga gaggatgacg aatttcgaca agaacctgcc
     3601 aaatgagaag gtgctcccta agcactcgct cctgtacgag tacttcacag tctacaacga
     3661 gctgactaag gtgaagtatg tgaccgaggg catgaggaag ccggctttcc tgtctgggga
     3721 gcagaagaag gccatcgtgg acctcctgtt caagaccaac cggaaggtca cggttaagca
     3781 gctcaaggag gactacttca agaagattga gtgcttcgat tcggtcgaga tctctggcgt
     3841 tgaggaccgc ttcaacgcct ccctggggac ctaccacgat ctcctgaaga tcattaagga
     3901 taaggacttc ctggacaacg aggagaatga ggatatcctc gaggacattg tgctgacact
     3961 cactctgttc gaggaccggg agatgatcga ggagcgcctg aagacttacg cccatctctt
     4021 cgatgacaag gtcatgaagc agctcaagag gaggaggtac accggctggg ggaggctgag
     4081 caggaagctc atcaacggca ttcgggacaa gcagtccggg aagacgatcc tcgacttcct
     4141 gaagagcgat ggcttcgcga accgcaattt catgcagctg attcacgatg acagcctcac
     4201 attcaaggag gatatccaga aggctcaggt gagcggccag ggggactcgc tgcacgagca
     4261 tatcgcgaac ctcgctggct cgccagctat caagaagggg attctgcaga ccgtgaaggt
     4321 tgtggacgag ctggtgaagg tcatgggcag gcacaagcct gagaacatcg tcattgagat
     4381 ggcccgggag aatcagacca cgcagaaggg ccagaagaac tcacgcgaga ggatgaagag
     4441 gatcgaggag ggcattaagg agctggggtc ccagatcctc aaggagcacc cggtggagaa
     4501 cacgcagctg cagaatgaga agctctacct gtactacctc cagaatggcc gcgatatgta
     4561 tgtggaccag gagctggata ttaacaggct cagcgattac gacgtcgatg ccatcgttcc
     4621 acagtcattc ctgaaggatg actccattga caacaaggtc ctcaccaggt cggacaagaa
     4681 ccggggcaag tctgataatg ttccttcaga ggaggtcgtt aagaagatga agaactactg
     4741 gcgccagctc ctgaatgcca agctgatcac gcagcggaag ttcgataacc tcacaaaggc
     4801 tgagaggggc gggctctctg agctggacaa ggcgggcttc atcaagaggc agctggtcga
     4861 gacacggcag atcactaagc acgttgcgca gattctcgac tcacggatga acactaagta
     4921 cgatgagaat gacaagctga tccgcgaggt gaaggtcatc accctgaagt caaagctcgt
     4981 ctccgacttc aggaaggatt tccagttcta caaggttcgg gagatcaaca attaccacca
     5041 tgcccatgac gcgtacctga acgcggtggt cggcacagct ctgatcaaga agtacccaaa
     5101 gctcgagagc gagttcgtgt acggggacta caaggtttac gatgtgagga agatgatcgc
     5161 caagtcggag caggagattg gcaaggctac cgccaagtac ttcttctact ctaacattat
     5221 gaatttcttc aagacagaga tcactctggc caatggcgag atccggaagc gccccctcat
     5281 cgagacgaac ggcgagacgg gggagatcgt gtgggacaag ggcagggatt tcgcgaccgt
     5341 caggaaggtt ctctccatgc cacaagtgaa tatcgtcaag aagacagagg tccagactgg
     5401 cgggttctct aaggagtcaa ttctgcctaa gcggaacagc gacaagctca tcgcccgcaa
     5461 gaaggactgg gatccgaaga agtacggcgg gttcgacagc cccactgtgg cctactcggt
     5521 cctggttgtg gcgaaggttg agaagggcaa gtccaagaag ctcaagagcg tgaaggagct
     5581 gctggggatc acgattatgg agcgctccag cttcgagaag aacccgatcg atttcctgga
     5641 ggcgaagggc tacaaggagg tgaagaagga cctgatcatt aagctcccca agtactcact
     5701 cttcgagctg gagaacggca ggaagcggat gctggcttcc gctggcgagc tgcagaaggg
     5761 gaacgagctg gctctgccgt ccaagtatgt gaacttcctc tacctggcct cccactacga
     5821 gaagctcaag ggcagccccg aggacaacga gcagaagcag ctgttcgtcg agcagcacaa
     5881 gcattacctc gacgagatca ttgagcagat ttccgagttc tccaagcgcg tgatcctggc
     5941 cgacgcgaat ctggataagg tcctctccgc gtacaacaag caccgcgaca agccaatcag
     6001 ggagcaggct gagaatatca ttcatctctt caccctgacg aacctcggcg cccctgctgc
     6061 tttcaagtac ttcgacacaa ctatcgatcg caagaggtac acaagcacta aggaggtcct
     6121 ggacgcgacc ctcatccacc agtcgattac cggcctctac gagacgcgca tcgacctgtc
     6181 tcagctcggg ggcgacgaat tctccgggag cgagacgcca ggcacctccg agtcggccac
     6241 cccagaatct gccacagtgg tgtccggcca aaagcaggac cgccagggcg gagaacgcag
     6301 aaggtcccag ctcgataggg atcagtgtgc ctactgcaag gagaagggcc actgggccaa
     6361 agactgcccg aaaaagccgc gcggcccacg cggcccaagg ccacaaacat ccctccttcc
     6421 aaagaagaag cggaaggtgg agctcagcgg aggatcttcc ggaggatcta gcggctccga
     6481 gacaccagga acatccgaaa gcgctacacc agaatctagc ggaggctctt ccggaggatc
     6541 taggcctacc ctcaacatcg aggatgagta tcgcctccac gaaacctcca aagaaccgga
     6601 cgtgtccctc ggcagcacat ggctcagcga cttcccacaa gcgtgggccg aaaccggcgg
     6661 catgggcctc gccgtccgcc aagccccact cattatcccg ctgaaggcga cctccacacc
     6721 ggtgtccatc aagcagtacc cgatgagcca agaggcgagg ctcgggatta agccgcacat
     6781 tcagcgcctc ctcgatcaag gcattctcgt gccgtgccaa tccccgtgga atacaccact
     6841 cctcccggtc aaaaagccgg gcaccaacga ctatcgcccg gtccaagatc tccgcgaggt
     6901 caacaagcgc gtggaagaca tccacccgac cgtcccgaac ccgtataatc tgctctccgg
     6961 gctcccacca tcccaccagt ggtatacagt gctggacctc aaagacgcct tcttctgtct
     7021 ccgcctccac ccaacaagcc agccgctctt cgccttcgag tggcgcgacc cggagatggg
     7081 catctccggc caactgacat ggacacgcct cccgcaaggc ttcaagaaca gcccgacact
     7141 cttcaacgag gcgctccata gggacctcgc ggattttcgc atccagcatc cggacctcat
     7201 cctcctccag tatgcggatg atctcctcct cgccgcgacc tccgagctgg attgtcaaca
     7261 aggcacacgc gcgctcctcc aaacactcgg gaacctcggc tatcgcgcgt ccgcgaaaaa
     7321 ggcccaaatc tgccagaagc aagtgaagta cctcgggtat ctgctcaagg aaggccaacg
     7381 ctggctcacc gaagcgcgca aagaaacagt gatggggcaa ccgacaccga aaacaccacg
     7441 ccagctgcgc gagtttctcg gcaaagccgg cttctgtcgc ctcttcatcc cgggctttgc
     7501 cgagatggcc gcgccactct acccactcac caagccgggc acactgttta actgggggcc
     7561 ggatcagcag aaagcctacc aagagatcaa acaagcgctc ctcaccgccc cagcgctcgg
     7621 gctcccagat ctcacaaagc cgttcgagct gttcgtcgat gagaagcaag gctacgcgaa
     7681 gggcgtgctc acacagaagc tcggcccgtg gaggaggcca gtggcctatc tctccaaaaa
     7741 actcgatcca gtggccgccg gctggccacc gtgtctgcgc atggtcgccg cgattgccgt
     7801 gctcacaaag gatgccggca aactcacaat gggccagccg ctggtgatcc tcgcgccaca
     7861 tgccgtggaa gccctcgtca aacagccgcc ggataggtgg ctctccaatg cgcgcatgac
     7921 ccattaccaa gcgctcctcc tcgacaccga tcgcgtccag ttcggcccag tggtcgccct
     7981 caatccggcg acactgctgc cactcccaga ggagggcctc caacacaact gtctggatat
     8041 tctcgcggaa gcgcatggca caaggccaga cctcacagat caaccgctca gcggcggcag
     8101 caagaggacc gccgactccc agcactcgac cccgccgaag accaagagga aggtggagtt
     8161 cgagccgaag aagaagagga aggtctccgg cggatcttcc ggaggatcta gcggctccga
     8221 gacaccagga acatccgaat ccgctacacc agagtcttct ggaggatcta gcggaggatc
     8281 tatgagtaat ctcttgaccg ttcatcaaaa tttaccggcg ctcccggtgg acgccacaag
     8341 cgatgaggtg cggaagaacc tgatggatat gttccgtgat cggcaggcgt ttagcgaaga
     8401 gacatggaag atgcttcttt ccgtgtgtcg tagttgggca gcgtggtgca agctcaacaa
     8461 ccggaagtgg ttccctgctg agccggaaga cgtcagggac tatttactct acctccaagc
     8521 tagggggtta gcagttaaaa ctatacagca gcatctcggt caattgaaca tgctgcaccg
     8581 gcgcagcgga ctgccgcggc ccagtgactc taatgctgtg tcgctcgtga tgaggcgcat
     8641 cagaaaggag aatgtggatg ctggtgaacg agccaagcag gcgcttgcct tcgagaggac
     8701 acagtttgat caagttcgca gcctcatgga gaacagcgat cgttgccaag acatcaggaa
     8761 tctagccttc ctgggtatcg cctacaatac tctgttgaga attgcggaga ttgcgaggat
     8821 ccgtgtgaag gacataagcc gaactgatgg tgggaggatg ttgattcaca ttggacgcac
     8881 caagacactc gtatcaaccg caggagtcga gaaggctcta tcgcttgggg ttactaagtt
     8941 ggtcgaacga tggatatcag tatccggcgt cgccgacgat ccaaacaatt acctgttctg
     9001 ccgcgtgcgc aagaatgggg ttgcggcgcc ttcagctacg tcccagctct cgacgagggc
     9061 actggagggc atctttgagg ctacgcacag gctgatctat ggcccgccgg atgattccgg
     9121 cgggagatac ttggcatggt ctggacactc cgccagagtc ggcgccgccc gcgacatggc
     9181 gcgggccggc gtgtcaattc cagaaataat gcaggcagga gggtggacca atgtcaacat
     9241 tgtaatgaac tatatcagaa acctcgactc ggagacagga gccatggtca ggcttctaga
     9301 agacggtgac ccagcagcta aacgcgtaaa gttggatgga ggtaagagga cagcggatgg
     9361 atctgaattc gagagcccga aaaagaagag gaaagtctga tgagcggccg cggtacgctg
     9421 aaatcaccag tctctctcta caaatctatc tctctctatt ttctccataa ataatgtgtg
     9481 agtagtttcc cgataaggga aattagggtt cttatagggt ttcgctcatg tgttgagcat
     9541 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
     9601 ttcctaaaac caaaatccag tactaaaatc cagatctcct aaagtcccta tagatctttg
     9661 tcgtgaatat aaaccagaca cgagacgact aaacctggag cccagacgcc gttcgaagct
     9721 agaagtaccg cttaggcagg aggccgttag ggaaaagatg ctaaggcagg gttggttacg
     9781 ttgactcccc cgtaggtttg gtttaaatat gatgaagtgg acggaaggaa ggaggaagac
     9841 aaggaaggat aaggttgcag gccctgtgca aggtaagaag atggaaattt gatagaggta
     9901 cgctactata cttatactat acgctaaggg aatgcttgta tttataccct atacccccta
     9961 ataacccctt atcaatttaa gaaataatcc gcataagccc ccgcttaaaa attggtatca
    10021 gagccatgaa taggtctatg accaaaactc aagaggataa aacctcacca aaatacgaaa
    10081 gagttcttaa ctctaaagat aaaagatctt tcaagatcaa aactagttcc ctcacaccgg
    10141 tgacggggat cgcatgcgat atctcgagat ctagcttggc gtaatcatgg tcatagctgt
    10201 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
    10261 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    10321 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
    10381 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
    10441 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    10501 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    10561 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    10621 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    10681 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    10741 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    10801 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    10861 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    10921 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    10981 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    11041 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    11101 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    11161 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    11221 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    11281 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    11341 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    11401 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    11461 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    11521 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    11581 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    11641 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    11701 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    11761 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    11821 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    11881 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    11941 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    12001 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    12061 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    12121 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    12181 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    12241 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    12301 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgccagtgcc aagctaattc
//
