LOCUS       sequence-486618-        8393 bp    DNA     circular SYN 17-SEP-2025
DEFINITION  Glutamate sensor for localization to membrane contatct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Shindo Y, Ashida K, Masamoto K, Takuwa H, Takahashi M, Higuchi M, 
            Ide R, Hotta K, Oka K
  TITLE     Genetically encoded sensors for analysing neurotransmission among 
            synaptically-connected neurons
  JOURNAL   bioRxiv 2022
REFERENCE   2  (bases 1 to 8393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022"
COMMENT     Sequence Label: pcDNA3.1-iRFP-P2A-Post-SPHERE-sfmTurquoise2-iG...
FEATURES             Location/Qualifiers
     source          1..8393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      917..926
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             923..1855
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHQQR"
     CDS             1862..1903
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1913..1969
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     3217..3238
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(3575..3586)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3984..4001)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3990..4214
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4260..4688
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4347..4366)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4557..4578
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4697..4717)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4702..5031
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4882..5017
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4944..4963
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5098..5892
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5152..5171)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5762..5781
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6066..6187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6103..6122)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6157..6176
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6236..6252)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6236..6252)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6249..6271)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6260..6276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6284..6290,6291..6308,6309..6314))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6329..6350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6467..6484)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6638..7226)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6718..6737)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7397..8188,8189..8257))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8020..8039
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8258..8362)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatggcgcg taaggtcgat ctcacctcct gcgatcgcga
      961 gccgatccac atccccggca gcattcagcc gtgcggctgc ctgctagcct gcgacgcgca
     1021 ggcggtgcgg atcacgcgca ttacggaaaa tgccggcgcg ttctttggac gcgaaactcc
     1081 gcgggtcggt gagctactcg ccgattactt cggcgagacc gaagcccatg cgctgcgcaa
     1141 cgcactggcg cagtcctccg atccaaagcg accggcgctg atcttcggtt ggcgcgacgg
     1201 cctgaccggc cgcaccttcg acatctcact gcatcgccat gacggtacat cgatcatcga
     1261 gttcgagcct gcggcggccg aacaggccga caatccgctg cggctgacgc ggcagatcat
     1321 cgcgcgcacc aaagaactga agtcgctcga agagatggcc gcacgggtgc cgcgctatct
     1381 gcaggcgatg ctcggctatc accgcgtgat gttgtaccgc ttcgcggacg acggctccgg
     1441 gatggtgatc ggcgaggcga agcgcagcga cctcgagagc tttctcggtc agcactttcc
     1501 ggcgtcgctg gtcccgcagc aggcgcggct actgtacttg aagaacgcga tccgcgtggt
     1561 ctcggattcg cgcggcatca gcagccggat cgtgcccgag cacgacgcct ccggcgccgc
     1621 gctcgatctg tcgttcgcgc acctgcgcag catctcgccc tgccatctcg aatttctgcg
     1681 gaacatgggc gtcagcgcct cgatgtcgct gtcgatcatc attgacggca cgctatgggg
     1741 attgatcatc tgtcatcatt acgagccgcg tgccgtgccg atggcgcagc gcgtcgcggc
     1801 cgaaatgttc gccgacttct tatcgctgca cttcaccgcc gcccaccaac aacgcggatc
     1861 cggcaagcct atccctaatc ctctgctggg actggactct acaggaagcg gagctactaa
     1921 cttcagcctg ctgaagcagg ctggagacgt ggaggagaac cctggacctg agaccgacac
     1981 cctcctgcta tgggtgctgc tgctctgggt gcccggcagc accggcgacg caccggtcgg
     2041 aggcaacgac cctaacctgt tcgtcgccct gtacgacttc gtcgcctctg gcgacaacac
     2101 cctgagcatc accaagggcg agaagctgag agtcctgggc tacaaccaca acggcgagtg
     2161 gtgcgaggcc cagaccaaga acggccaggg ctgggtccct agcaactaca tcacacctgt
     2221 caacagcacc ggtggaggca gcggaggagg ctccggagcc gcaggcagca cgctggacaa
     2281 aatcgccaaa aacggtgtga ttgtcgtcgg tcaccgtgaa tcttcagtgc ctttctctta
     2341 ttacgacaat cagcaaaaag tggtgggtta ctcgcaggat tactccaacg ccattgttga
     2401 agcagtgaaa aagaaactca acaaaccgga cttgcaggta aaactgattc cgattacctc
     2461 acaaaaccgt attccactgc tgcaaaacgg cactttcgat tttgaatgtg gttctaccac
     2521 caacaacgtc gaacgccaaa aacaggcggc tttctctgac actattttcg tggtcggtac
     2581 gcgcctgttg accaaaaagg gtggcgatat caaagatttt gccaacctga aagacaaagc
     2641 cgtagtcgtc acttccggca ctacctctga agttttgctc aacaaactga atgaagagca
     2701 aaaaatgaat atgcgcatca tcagcgccaa agatcacggt gactctttcc gcaccctgga
     2761 aagcggtcgt gccgttgcct ttatgatgga tgacgtgctg ctggccggtg aacgtgcgaa
     2821 agcgaagaaa ccagacaact gggaaatcgt cggcaagccg cagtctcagg aggcctacgg
     2881 ttgtatgttg cgtaaagatg atccgcagtt caaaaagctg atggatgaca ccatcgctca
     2941 ggtgcagacc tccggtgaag cggaaaaatg gtttgataag tggttcaaaa atccaatttg
     3001 gggcttccac aacgtctata tcaccgccga caagcagaag aacggcatca aggcgaactt
     3061 caagatccgc cacaacgtgg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3121 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3181 cgtgctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3241 cgccgccggg atcactctcg gcatggacga gctgtacaag ggaggaggct ccggaggcgg
     3301 aagcggtacc ctcgagctgg tgccccactt gcataacctc aatgacatca gccaatacac
     3361 aagtacaacc accaaggtcc caagcactga catcaccctt cgaccaactc ggaaaaactc
     3421 aacccctgtg accagcgcat ttccaaccgc taagcaagac gaccccaaac aacagcctag
     3481 tccatttagc gtagaccaaa gggactacag cacagagctg tccgtgacca tagctgtagg
     3541 agcttccctg cttttcctga acattctggc tttcgcggcc ctctattaca agaaggacaa
     3601 aaggcgccac gacgtacata ggagatgtag tccgcaacga actacaacca atgatcttac
     3661 gcacgcacct gaggaggaga tcatgagtct tcagatgaaa cacacagacc tggaccacga
     3721 gtgtgaatcc atccatcccc atgaggtggt gctgaggaca gcatgccctc cggactatac
     3781 cctggcaatg cgccgaagcc cagacgacat tcccctgatg acacccaata ccataaccat
     3841 gattcccaac actattccag gaatccagcc tctccacact ttcaacacat tcactggtgg
     3901 acagaataat actctccctc atccgcatcc acacccccac tcccacagct aatctagagg
     3961 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4021 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4081 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4141 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4201 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     4261 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4321 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4381 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4441 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4501 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4561 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4621 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4681 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4741 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4801 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4861 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4921 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     4981 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     5041 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     5101 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     5161 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5221 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     5281 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5341 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5401 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5461 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5521 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     5581 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     5641 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5701 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5761 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     5821 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     5881 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     5941 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     6001 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     6061 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6121 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6181 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     6241 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6301 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6361 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6421 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6481 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6541 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6601 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6661 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6721 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6781 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6841 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6901 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6961 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7021 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7081 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7141 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7201 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7261 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7321 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7381 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7441 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7501 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7561 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7621 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7681 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7741 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7801 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7861 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7921 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7981 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8041 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8101 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8161 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8221 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8281 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8341 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
