LOCUS       sequence-486660-        6736 bp    DNA     circular SYN 18-SEP-2025
DEFINITION  Helper plasmid to produce CHIKV-pseudotyped propogation incompetent 
            Sindbis virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Kim H, Xu C, Washington C, Lowmann M, Kebschull JM
  TITLE     Mapping the projectional architecture of the mouse midbrain 
            dopaminergic system using cell type-specific barcoding
  JOURNAL   bioRxiv 2025.06.25.661405; doi: 
            https://doi.org/10.1101/2025.06.25.661405
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.25.661405; doi: https://doi.org/10.1101/2025.06.25.661405"
COMMENT     Sequence Label: DH-BB[5'SIN;181/25ORF]
FEATURES             Location/Qualifiers
     source          1..6736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2902..3006
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3007..3075,3076..3867)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3225..3244)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4038..4626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4527..4546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        4714..4732
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4714..4731
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 acacttcatc ctggcccgat gtccgaaagg agaaactctg acggtgggat tcactgacgg
       61 taggaagatc agtcactcat gtacgcaccc atttcaccac gaccctcctg tgataggccg
      121 ggaaaaattt cattcccgac cgcagcacgg tagagaacta ccttgcagca cgtacgcgca
      181 gagcaccgct gcaactgccg aggagataga ggtacatatg cccccagaca ccccagatcg
      241 cacattgatg tcacaacagt ccggtaatgt aaagatcaca gtcaatagtc agacggtgcg
      301 gtacaagtgt aattgcggtg actcaaatga aggactaacc actacagaca aagtgattaa
      361 taactgcaag gttgatcaat gccatgccgc ggtcaccaat cacaaaaaat ggcagtataa
      421 ttcccctctg gtcccgcgta atgctgaact cggggaccga aaaggaaaag ttcacattcc
      481 gtttcctctg gcaaatgtga catgcagggt gcctaaggca aggaacccca ccgtgacgta
      541 cggaaaaaac caagtcatca tgctgctgta tcctgaccac ccaacgctcc tgtcctaccg
      601 gaatatggga gaagaaccaa actatcaaga agagtgggtg acgcataaga aggagatcag
      661 gttaaccgtg ccgactgaag ggctagaggt cacgtggggc aacaacgagc cgtacaagta
      721 ttggccgcag ttatccacaa acggtacagc ccacggccac ccgcatgaga taattttgta
      781 ttattatgag ctgtacccta ctatgactgt ggtagttgtg tcagtggcct cgttcgtact
      841 cctgtcgatg gtgggtgtgg cagtggggat gtgcatgtgt gcacgacgca gatgcattac
      901 accgtacgaa ctgacaccag gagctaccgt ccctttcctg cttagcctaa tatgctgcat
      961 tagaacagct aaagcggcca cataccaaga ggctgcggta tacctgtgga acgagcagca
     1021 gcctttgttt tggctgcaag cccttattcc gctggcagcc ctgattgtcc tatgcaactg
     1081 tctgagactc ttaccatgct tttgtaaaac gttgactttt ttagccgtaa tgagcgtcgg
     1141 tgcccacact gtgagcgcgt acgaacacgt aacagtgatc ccgaacacgg tgggagtacc
     1201 gtataagact ctagtcaaca gaccgggcta cagccccatg gtactggaga tggagcttct
     1261 gtcagtcact ttggagccaa cgctatcgct tgattacatc acgtgcgagt ataaaaccgt
     1321 catcccgtct ccgtacgtga aatgctgcgg tacagcagag tgcaaggaca agagcctacc
     1381 tgattacagc tgtaaggtct tcaccggcgt ctacccattc atgtggggcg gcgcctactg
     1441 cttctgcgac actgaaaata cgcaattgag cgaagcacat gtggagaagt ccgaatcatg
     1501 caaaacagaa tttgcatcag catatagggc tcataccgca tccgcatcag ctaagctccg
     1561 cgtcctttac caaggaaata atgttactgt atctgcttat gcaaacggcg atcatgccgt
     1621 cacagttaag gacgctaaat tcattgtggg gccaatgtct tcagcctgga caccttttga
     1681 caataaaatc gtggtgtaca aaggcgacgt ctacaacatg gactacccgc ccttcggcgc
     1741 aggaagacca ggacaatttg gcgacatcca aagtcgcacg cctgagagcg aagacgtcta
     1801 tgctaacaca caactggtac tgcagagacc gtccgcgggt acggtgcacg tgccgtactc
     1861 tcaggcacca tctggcttca agtattggct aaaagaacga ggggcgtcgc tgcagcacac
     1921 agcaccattt ggctgtcaaa tagcaacaaa cccggtaaga gcgatgaact gcgccgtagg
     1981 gaacatgcct atctccatcg acataccgga cgcggccttc actagggtcg tcgacgcgcc
     2041 atctttaacg gacatgtcgt gtgaggtacc agcctgcacc cactcctcag actttggggg
     2101 cgtagccatc attaaatatg cagccagcaa gaaaggcaag tgtgcggtgc attcgatgac
     2161 taacgccgtc actattcggg aagctgaaat agaagtagaa gggaactctc agttgcaaat
     2221 ctctttttcg acggccctag ccagcgccga attccgcgta caagtctgtt ctacacaagt
     2281 acactgtgca gccgagtgcc atccaccgaa agaccatata gtcaattacc cggcgtcaca
     2341 caccaccctc ggggtccaag acatttccgt tacggcgatg tcatgggtgc agaagatcac
     2401 gggaggtgtg ggactggttg tcgctgttgc agcactgatc ctaatcgtgg tgctatgcgt
     2461 gtcgtttagc aggcactaac cgctacgccc caatgatccg accagcaaaa ctcgatgtac
     2521 ttccgaggaa ctgatgtgca taatgcatca ggctggtaca ttagatcccc gcttaccgcg
     2581 ggcaatatag caacactaaa aactcgatgt acttccgagg aagcgcagtg cataatgctg
     2641 cgcagtgttg ccacataacc actatattaa ccatttatct agcggacgcc aaaaactcaa
     2701 tgtatttctg aggaagcgtg gtgcataatg ccacgcagcg tctgcataac ttttattatt
     2761 tcttttatta atcaacaaaa ttttgttttt aacatttcaa aaaaaaaaaa aaaaaaaaaa
     2821 aaaaaaaaaa aaggggaatt cctcgagggg aattaattct tgaagacgaa agggccaggt
     2881 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     2941 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3001 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3061 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3121 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3181 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3241 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3301 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     3361 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     3421 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     3481 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3541 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     3601 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     3661 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     3721 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     3781 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     3841 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     3901 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3961 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4021 aagattaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4081 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4141 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     4201 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4261 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4321 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4381 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4441 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4501 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4561 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4621 tggaaaaacg ccagcaacgc gagctcgtat ggacatattg tcgttagaac gcggctacaa
     4681 ttaatacata accttatgta tcatacacat acgatttagg tgacactata gattgacggc
     4741 gtagtacaca ctattgaatc aaacagccga ccaattgcac taccatcaca atggagaagc
     4801 cagtagtaaa cgtagacgta gacccccaga gtccgtttgt cgtgcaactg caaaaaagct
     4861 tcccgcaatt tgaggtagta gcacagcagg tcactccaaa tgaccatgct aatgccagag
     4921 cattttcgca tctggccagt aaactaatcg agctggaggt tcctaccaca gcgacgatct
     4981 tggacatagg cagcgcaccg gctcgtagaa tgttttccga gcaccagtat cattgtgtct
     5041 gccccatgcg tagtccagaa gacccggacc gcatgatgaa atatgccagt aaactggcgg
     5101 aaaaagcgtg caagattaca aacaagaact tgcatgagaa gattaaggat ctccgggatc
     5161 ccctgaaaag gctgtttaag ttgggtaaac cgctcccagc cgacgacgag caagacgaag
     5221 acagaagacg cgctctgcta gatgaaacaa aggcgtggtt tagagtaggt ataacaggca
     5281 ctttagcagt ggccgtgacg acccggtatg aggtagacaa tattacacct gtcctactgg
     5341 cattgagaac ttttgcccag agcaaaagag cattccaagc catcagaggg gaaataaagc
     5401 atctctacgg tggtcctaaa tagtcagcat agtacatttc atctgactaa tactacaaca
     5461 ccaccaccat ggagtttatc ccaacccaaa ctttctacaa taggaggtac cagcctcgac
     5521 cttggactcc gcgccctact atccaagtta tcagacccag accgcgtccg caaaggaaag
     5581 ccgggcaact tgcccagctg atctcagcag ttaataaact gacaatgcgc gcggtacctc
     5641 aacagaagcc gcgcaagaat cggaagaata agaagcaaaa gcaaaagcag caggcgccac
     5701 gaaacaacat gaatcaaaag aagcagcccc ctaaaaagaa accggctcaa aagaaaaaga
     5761 agccgggccg tagagagaga atgtgcatga aaatcgaaaa tgattgcatc ttcgaagtca
     5821 agcatgaagg taaggtaaca ggttacgcgt gcttggtagg ggacaaagta atgaagccag
     5881 cacacgtaaa ggggaccatc gataatgcgg acctggccaa attggccttc aagcggtcat
     5941 ctaagtacga ccttgaatgc gcgcagatac ccgtgcacat gaagtccgac gcttcgaagt
     6001 tcacccatga gaaaccggag gggtactaca actggcacca cggagcagta cagtactcag
     6061 gaggccggtt caccatccct acaggtgcgg gcaaaccagg ggacagcggt agaccgatct
     6121 tcgacaacaa ggggcgcgtg gtggccatag ttttaggagg agctaatgaa ggagcccgta
     6181 cagccctctc ggtggtgacc tggaacaaag acatcgtcac gaaaatcacc cctgaggggg
     6241 ccgaagagtg gagtcttgcc attccagtta tgtgcctgct ggcaaatacc acgttcccct
     6301 gctcccagcc cccttgcaca ccctgctgct acgaaaaaga gccggagaaa accctgcgca
     6361 tgctagaaga caacgtcatg agccccgggt actatcagct gctacaagca tccttaacat
     6421 gttctccccg ccgccagcga cgcagtatta aggacaactt caatgtctat aaagccataa
     6481 gaccgtacct agctcactgt cccgactgtg gagaagggca ctcgtgccat agtcccgtag
     6541 cgctagaacg catcagaaac gaagcgacag acgggacgct gaaaatccag gtttccttgc
     6601 aaatcggaat aaagacggat gatagccatg attggaccaa gctgcgttac atggacaatc
     6661 atatgccagc agacgcagag agggccaggc tatttgtaag aacgtcagca ccgtgcacga
     6721 ttactggaac aatggg
//
