LOCUS       sequence-486661-        9945 bp    DNA     circular SYN 18-SEP-2025
DEFINITION  24-stem loop MS2 Citrine + magnetic separation RNA reporter, 
            stem-loops in 5'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9945)
  AUTHORS   Thurm AR, Finkel Y, Andrews C, Cai XS, Benko C, Bintu L
  TITLE     High-throughput discovery of regulatory effector domains in human 
            RNA-binding proteins.
  JOURNAL   bioRxiv [Preprint]. 2024 Jul 19:2024.07.19.604317. doi: 
            10.1101/2024.07.19.604317.
  PUBMED    39071298
REFERENCE   2  (bases 1 to 9945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jul 19:2024.07.19.604317. doi: 
            10.1101/2024.07.19.604317."
COMMENT     Sequence Label: pAT039
FEATURES             Location/Qualifiers
     source          1..9945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     491..510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     744..761
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    878..899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(914..919,920..937,938..944)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    952..968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     957..979
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     976..992
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     976..992
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1013..1031
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1066..1869
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    1876..1901
                     /label=SA
                     /note="splice acceptor site"
     CDS             1925..1978
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1988..2587
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1988..2007)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2484..2504
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(2619..2636)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2625..2849
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2922..2940
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2958..2976
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2994..3012
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3030..3048
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3066..3084
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3102..3120
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3138..3156
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3174..3192
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3210..3228
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          3473..4411
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4368..4388
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        4444..4462
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(4472..4483)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      5819..5828
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     5825..5887
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     CDS             6656..6685
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6689..6835
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             6851..6904
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      6911..6920
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6917..6919,6920..6922,6923..7633)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6962..6983)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7223..7242)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7570..7591
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7634..7654
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7665..7786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7702..7721)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7756..7775
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    7852..8688
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     CDS             complement(join(8963..9754,9755..9823))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9586..9605
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
       61 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      781 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      841 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      901 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      961 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc agaattaacc
     1021 ctcactaaag ggactagtcc tgcaggttta aaccaattgg ccctttgctt tctctgacca
     1081 gcattctctc ccctgggcct gtgccgcttt ctgtctgcag cttgtggcct gggtcacctc
     1141 tacggctggc ccagatcctt ccctgccgcc tccttcaggt tccgtcttcc tccactccct
     1201 cttccccttg ctctctgctg tgttgctgcc caaggatgct ctttccggag cacttccttc
     1261 tcggcgctgc accacgtgat gtcctctgag cggatcctcc ccgtgtctgg gtcctctccg
     1321 ggcatctctc ctccctcacc caaccccatg ccgtcttcac tcgctgggtt cccttttcct
     1381 tctccttctg gggcctgtgc catctctcgt ttcttaggat ggccttctcc gacggatgtc
     1441 tcccttgcgt cccgcctccc cttcttgtag gcctgcatca tcaccgtttt tctggacaac
     1501 cccaaagtac cccgtctccc tggctttagc cacctctcca tcctcttgct ttctttgcct
     1561 ggacaccccg ttctcctgtg gattcgggtc acctctcact cctttcattt gggcagctcc
     1621 cctacccccc ttacctctct agtctgtgct agctcttcca gccccctgtc atggcatctt
     1681 ccaggggtcc gagagctcag ctagtcttct tcctccaacc cgggccccta tgtccacttc
     1741 aggacagcat gtttgctgcc tccagggatc ctgtgtcccc gagctgggac caccttatat
     1801 tcccagggcc ggttaatgtg gctctggttc tgggtacttt tatctgtccc ctccacccca
     1861 cagtggggca agcttctgac ctcttctctt cctcccacag ggcctcgaga gatcaggcag
     1921 cggagagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctag
     1981 gctcgagatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     2041 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     2101 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     2161 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     2221 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     2281 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     2341 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     2401 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     2461 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     2521 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     2581 tgcctgatct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     2641 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     2701 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     2761 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     2821 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctgggggct
     2881 agcaaagcct aggaacagct gcatatagca ctgcagttta ctccctatca gtgatagaga
     2941 gtcaaaaggc catgccctcc ctatcagtga tagagagagc agactcaagg tggtccctat
     3001 cagtgataga gagattgagc aagagggcct ccctatcagt gatagagaat agcactgcag
     3061 tttactccct atcagtgata gagagtcaaa aggccatgcc ctccctatca gtgatagaga
     3121 gagcagactc aaggtggtcc ctatcagtga tagagagatt gagcaagagg gcctccctat
     3181 cagtgataga gattgatcca gtcagggcct ccctatcagt gatagagaag ctatgtgcag
     3241 gtagggctgt acagtgggaa tgcatgagcg cacatcgccc acagtccccg agaagttggg
     3301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     3361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     3421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
     3481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
     3541 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
     3601 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
     3661 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
     3721 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
     3781 gcaagatagt cttgtaaatg cgggccaaca tgtgcacact ggtatttcgg tttttggggc
     3841 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
     3901 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
     3961 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     4021 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     4081 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     4141 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     4201 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     4261 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     4321 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     4381 agtggttcaa agtttttttc ttccatttca ggtgtcgtga gctagcgatt acgccaagct
     4441 cgaaattaac cctcactaaa gggaacaaaa gctggagctc ccaccgcggt gggcggccgc
     4501 gaattcgccc ttcgcgggat cctacggtac ttattgccaa gaaagcacga gcatcagccg
     4561 tgcctccagg tcgaatcttc aaacgacgac gatcacgcgt cgctccagta ttccagggtt
     4621 catcagatcc tacggtactt attgccaaga aagcacgagc atcagccgtg cctccaggtc
     4681 gaatcttcaa acgacgacga tcacgcgtcg ctccagtatt ccagggttca tcagatccta
     4741 cggtacttat tgccaagaaa gcacgagcat cagccgtgcc tccaggtcga atcttcaaac
     4801 gacgacgatc acgcgtcgct ccagtattcc agggttcatc agatcctacg gtacttattg
     4861 ccaagaaagc acgagcatca gccgtgcctc caggtcgaat cttcaaacga cgacgatcac
     4921 gcgtcgctcc agtattccag ggttcatcag atcctacggt acttattgcc aagaaagcac
     4981 gagcatcagc cgtgcctcca ggtcgaatct tcaaacgacg acgatcacgc gtcgctccag
     5041 tattccaggg ttcatcagat cctacggtac ttattgccaa gaaagcacga gcatcagccg
     5101 tgcctccagg tcgaatcttc aaacgacgac gatcacgcgt cgctccagta ttccagggtt
     5161 catcagatcc tacggtactt attgccaaga aagcacgagc atcagccgtg cctccaggtc
     5221 gaatcttcaa acgacgacga tcacgcgtcg ctccagtatt ccagggttca tcagatccta
     5281 cggtacttat tgccaagaaa gcacgagcat cagccgtgcc tccaggtcga atcttcaaac
     5341 gacgacgatc acgcgtcgct ccagtattcc agggttcatc agatcctacg gtacttattg
     5401 ccaagaaagc acgagcatca gccgtgcctc caggtcgaat cttcaaacga cgacgatcac
     5461 gcgtcgctcc agtattccag ggttcatcag atcctacggt acttattgcc aagaaagcac
     5521 gagcatcagc cgtgcctcca ggtcgaatct tcaaacgacg acgatcacgc gtcgctccag
     5581 tattccaggg ttcatcagat cctacggtac ttattgccaa gaaagcacga gcatcagccg
     5641 tgcctccagg tcgaatcttc aaacgacgac gatcacgcgt cgctccagta ttccagggtt
     5701 catcagatcc tacggtactt attgccaaga aagcacgagc atcagccgtg cctccaggtc
     5761 gaatcttcaa acgacgacga tcacgcgtcg ctccagtatt ccagggttca tcagatctgc
     5821 caccatggag acagacacac tcctgctatg ggtactgctg ctctgggttc caggttccac
     5881 tggtgacgct agcgagacgg gcggatcagg cggtcgtctc ggatccgaca aaactcacac
     5941 atgcccaccg tgcccagcac ctgaactcct ggggggaccg tcagtcttcc tcttcccccc
     6001 aaaacccaag gacaccctca tgatctcccg gacccctgag gtcacatgcg tggtggtgga
     6061 cgtgagccac gaagaccctg aggtcaagtt caactggtac gtggacggcg tggaggtgca
     6121 taatgccaag acaaagccgc gggaggagca gtacaacagc acgtaccgtg tggtcagcgt
     6181 cctcaccgtc ctgcaccagg actggctgaa tggcaaggag tacaagtgca aggtctccaa
     6241 caaagccctc ccagccccca tcgagaaaac catctccaaa gccaaagggc agccccgaga
     6301 accacaggtc tacaccctgc ccccatcccg ggaggagatg accaagaacc aggtcagcct
     6361 gacctgcctg gtcaaaggct tctatcccag cgacatcgcc gtggagtggg agagcaatgg
     6421 gcagccggag aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt
     6481 cctctacagc aagctcaccg tggacaagag caggtggcag caggggaacg tcttctcatg
     6541 ctccgtgatg cacgaggctc tgcacaacca ctacacgcag aagagcctct ccctgtctcc
     6601 gggtaaagga ggaggtggaa gcggaggagg aggaagcgga ggaggaggta gcggagaaca
     6661 aaaactcatc tcagaagagg atctgaatgc tgtgggccag gacacgcagg aggtcatcgt
     6721 ggtgccacac tccttgccct ttaaggtggt ggtgatctca gccatcctgg ccctggtggt
     6781 gctcaccatc atctccctta tcatcctcat catgctttgg cagaagaagc cacgttgtac
     6841 aggcagtgga gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg
     6901 cccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     6961 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     7021 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     7081 cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgatgtgct tcgcccgcta
     7141 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     7201 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     7261 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     7321 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     7381 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     7441 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     7501 gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa
     7561 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     7621 cgagctgtac aagccaaaaa agaagagaaa ggtatagtta actaaacttg tttattgcag
     7681 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7741 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgcatat
     7801 gtaaattgta agcgtctcgg cctctagagt cgagatgaca ttgattattg aactagggac
     7861 aggattggtg acagaaaagc cccatcctta ggcctcctcc ttcctagtct cctgatattg
     7921 ggtctaaccc ccacctcctg ttaggcagat tccttatctg gtgacacacc cccatttcct
     7981 ggagccatct ctctccttgc cagaacctct aaggtttgct tacgatggag ccagagagga
     8041 tcctgggagg gagagcttgg cagggggtgg gagggaaggg ggggatgcgt gacctgcccg
     8101 gttctcagtg gccaccctgc gctaccctct cccagaacct gagctgctct gacgcggctg
     8161 tctggtgcgt ttcactgatc ctggtgctgc agcttcctta cacttcccaa gaggagaagc
     8221 agtttggaaa aacaaaatca gaataagttg gtcctgagtt ctaactttgg ctcttcacct
     8281 ttctagtccc caatttatat tgttcctccg tgcgtcagtt ttacctgtga gataaggcca
     8341 gtagccagcc ccgtcctggc agggctgtgg tgaggagggg ggtgtccgtg tggaaaactc
     8401 cctttgtgag aatggtgcgt cctaggtgtt caccaggtcg tggccgcctc tactcccttt
     8461 ctctttctcc atccttcttt ccttaaagag tccccagtgc tatctgggac atattcctcc
     8521 gcccagagca gggtcccgct tccctaaggc cctgctctgg gcttctgggt ttgagtcctt
     8581 ggcaagccca ggagaggcgc tcaggcttcc ctgtccccct tcctcgtcca ccatctcatg
     8641 cccctggctc tcctgcccct tccctacagg ggttcctggc tctgctcttc gccttcttga
     8701 cgagttcttc tgaattatta acgcttacaa tttcctgatg cggtattttc tccttacgca
     8761 tctgtgcggt atttcacacc gcatcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8821 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga ttatcaaaaa
     8881 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8941 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9001 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9061 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9121 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9181 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9241 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9301 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9361 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9421 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9481 tgccatccgt aagatgcttt tctgtgactg gtgartactc aaccaagtca ttctgagaat
     9541 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9601 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9661 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9721 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9781 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9841 attattgaag catttatcag ggttattgtc tcatgaccaa aatcccttaa cgtgagtttt
     9901 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atctt
//
