LOCUS       sequence-486664-        8984 bp    DNA     circular SYN 18-SEP-2025
DEFINITION  7-stem loop MS2 Citrine + magnetic separation RNA reporter, 
            stem-loops in 5'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8984)
  AUTHORS   Thurm AR, Finkel Y, Andrews C, Cai XS, Benko C, Bintu L
  TITLE     High-throughput discovery of regulatory effector domains in human 
            RNA-binding proteins.
  JOURNAL   bioRxiv [Preprint]. 2024 Jul 19:2024.07.19.604317. doi: 
            10.1101/2024.07.19.604317.
  PUBMED    39071298
REFERENCE   2  (bases 1 to 8984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jul 19:2024.07.19.604317. doi: 
            10.1101/2024.07.19.604317."
COMMENT     Sequence Label: pAT209
FEATURES             Location/Qualifiers
     source          1..8984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1065..1868
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    1875..1900
                     /label=SA
                     /note="splice acceptor site"
     CDS             1924..1977
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1987..2586
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1987..2006)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2483..2503
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(2618..2635)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2624..2848
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2921..2939
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2957..2975
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2993..3011
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3029..3047
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3065..3083
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3101..3119
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3137..3155
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3173..3191
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3209..3227
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          3472..4410
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4367..4387
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        4443..4461
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(4471..4482)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      4857..4866
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     4863..4925
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     CDS             5694..5723
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5727..5873
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             5889..5942
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      5949..5958
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5955..5957,5958..5960,5961..6671)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6000..6021)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6261..6280)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6608..6629
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6672..6692
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6703..6824
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6740..6759)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6794..6813
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    6890..7726
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     CDS             complement(join(8001..8792,8793..8861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8624..8643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca gaattaaccc
     1021 tcactaaagg gactagtcct gcaggtttaa accaattggc cctttgcttt ctctgaccag
     1081 cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg ggtcacctct
     1141 acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct ccactccctc
     1201 ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc acttccttct
     1261 cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg tcctctccgg
     1321 gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc ccttttcctt
     1381 ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg acggatgtct
     1441 cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt ctggacaacc
     1501 ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt tctttgcctg
     1561 gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg ggcagctccc
     1621 ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca tggcatcttc
     1681 caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat gtccacttca
     1741 ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc accttatatt
     1801 cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc tccaccccac
     1861 agtggggcaa gcttctgacc tcttctcttc ctcccacagg gcctcgagag atcaggcagc
     1921 ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctagg
     1981 ctcgagatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     2041 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     2101 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     2161 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     2221 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     2281 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     2341 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     2401 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     2461 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     2521 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     2581 gcctgatcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     2641 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     2701 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     2761 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     2821 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctgggggcta
     2881 gcaaagccta ggaacagctg catatagcac tgcagtttac tccctatcag tgatagagag
     2941 tcaaaaggcc atgccctccc tatcagtgat agagagagca gactcaaggt ggtccctatc
     3001 agtgatagag agattgagca agagggcctc cctatcagtg atagagaata gcactgcagt
     3061 ttactcccta tcagtgatag agagtcaaaa ggccatgccc tccctatcag tgatagagag
     3121 agcagactca aggtggtccc tatcagtgat agagagattg agcaagaggg cctccctatc
     3181 agtgatagag attgatccag tcagggcctc cctatcagtg atagagaagc tatgtgcagg
     3241 tagggctgta cagtgggaat gcatgagcgc acatcgccca cagtccccga gaagttgggg
     3301 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     3361 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     3421 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     3481 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     3541 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     3601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     3661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     3721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     3781 caagatagtc ttgtaaatgc gggccaacat gtgcacactg gtatttcggt ttttggggcc
     3841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     4021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     4081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     4141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     4201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     4261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     4321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     4381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag ctagcgatta cgccaagctc
     4441 gaaattaacc ctcactaaag ggaacaaaag ctggagctcc caccgcggtg ggctccgttt
     4501 atgctttcca gctcggtatc agtgcacgag catccgccgt gccttaacaa ggctaacaaa
     4561 cgactcgcac gagcatcagc cgtgcctgag cccttactcc gtggtctcgc acgagcatca
     4621 gccgtgccta tcatacaatt ccactgtatt tgcacgagca tcagccgtgc ctggccatta
     4681 acacccaaac agtggcacga gcatcagccg tgcctcaggc ctatgttcca gacggatgca
     4741 cgagcatcag ccgtgcctag aggcgagtcc tgcgcgaatc gcacgagcat cagccgtgcc
     4801 tgaaccgaag tccttggagt ttagagcgag ggccgctcga ctagctacgt agatctgcca
     4861 ccatggagac agacacactc ctgctatggg tactgctgct ctgggttcca ggttccactg
     4921 gtgacgctag cgagacgggc ggatcaggcg gtcgtctcgg atccgacaaa actcacacat
     4981 gcccaccgtg cccagcacct gaactcctgg ggggaccgtc agtcttcctc ttccccccaa
     5041 aacccaagga caccctcatg atctcccgga cccctgaggt cacatgcgtg gtggtggacg
     5101 tgagccacga agaccctgag gtcaagttca actggtacgt ggacggcgtg gaggtgcata
     5161 atgccaagac aaagccgcgg gaggagcagt acaacagcac gtaccgtgtg gtcagcgtcc
     5221 tcaccgtcct gcaccaggac tggctgaatg gcaaggagta caagtgcaag gtctccaaca
     5281 aagccctccc agcccccatc gagaaaacca tctccaaagc caaagggcag ccccgagaac
     5341 cacaggtcta caccctgccc ccatcccggg aggagatgac caagaaccag gtcagcctga
     5401 cctgcctggt caaaggcttc tatcccagcg acatcgccgt ggagtgggag agcaatgggc
     5461 agccggagaa caactacaag accacgcctc ccgtgctgga ctccgacggc tccttcttcc
     5521 tctacagcaa gctcaccgtg gacaagagca ggtggcagca ggggaacgtc ttctcatgct
     5581 ccgtgatgca cgaggctctg cacaaccact acacgcagaa gagcctctcc ctgtctccgg
     5641 gtaaaggagg aggtggaagc ggaggaggag gaagcggagg aggaggtagc ggagaacaaa
     5701 aactcatctc agaagaggat ctgaatgctg tgggccagga cacgcaggag gtcatcgtgg
     5761 tgccacactc cttgcccttt aaggtggtgg tgatctcagc catcctggcc ctggtggtgc
     5821 tcaccatcat ctcccttatc atcctcatca tgctttggca gaagaagcca cgttgtacag
     5881 gcagtggaga gggcagagga agtctgctaa catgcggtga cgtcgaggag aatcctggcc
     5941 caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     6001 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     6061 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     6121 tgccctggcc caccctcgtg accaccttcg gctacggcct gatgtgcttc gcccgctacc
     6181 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     6241 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     6301 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     6361 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     6421 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     6481 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     6541 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
     6601 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     6661 agctgtacaa gccaaaaaag aagagaaagg tatagttaac taaacttgtt tattgcagct
     6721 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6781 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgcatatgt
     6841 aaattgtaag cgtctcggcc tctagagtcg agatgacatt gattattgaa ctagggacag
     6901 gattggtgac agaaaagccc catccttagg cctcctcctt cctagtctcc tgatattggg
     6961 tctaaccccc acctcctgtt aggcagattc cttatctggt gacacacccc catttcctgg
     7021 agccatctct ctccttgcca gaacctctaa ggtttgctta cgatggagcc agagaggatc
     7081 ctgggaggga gagcttggca gggggtggga gggaaggggg ggatgcgtga cctgcccggt
     7141 tctcagtggc caccctgcgc taccctctcc cagaacctga gctgctctga cgcggctgtc
     7201 tggtgcgttt cactgatcct ggtgctgcag cttccttaca cttcccaaga ggagaagcag
     7261 tttggaaaaa caaaatcaga ataagttggt cctgagttct aactttggct cttcaccttt
     7321 ctagtcccca atttatattg ttcctccgtg cgtcagtttt acctgtgaga taaggccagt
     7381 agccagcccc gtcctggcag ggctgtggtg aggagggggg tgtccgtgtg gaaaactccc
     7441 tttgtgagaa tggtgcgtcc taggtgttca ccaggtcgtg gccgcctcta ctccctttct
     7501 ctttctccat ccttctttcc ttaaagagtc cccagtgcta tctgggacat attcctccgc
     7561 ccagagcagg gtcccgcttc cctaaggccc tgctctgggc ttctgggttt gagtccttgg
     7621 caagcccagg agaggcgctc aggcttccct gtcccccttc ctcgtccacc atctcatgcc
     7681 cctggctctc ctgccccttc cctacagggg ttcctggctc tgctcttcgc cttcttgacg
     7741 agttcttctg aattattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc
     7801 tgtgcggtat ttcacaccgc atcaggtggc acttttcggg gaaatgtgcg cggaacccct
     7861 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagatt atcaaaaagg
     7921 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7981 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8041 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8101 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8161 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8221 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8281 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8341 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8401 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8461 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8521 ccatccgtaa gatgcttttc tgtgactggt gartactcaa ccaagtcatt ctgagaatag
     8581 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8641 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8701 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8761 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8821 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8881 tattgaagca tttatcaggg ttattgtctc atgaccaaaa tcccttaacg tgagttttcg
     8941 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
