LOCUS       sequence-486690-        6159 bp    DNA     circular SYN 18-SEP-2025
DEFINITION  Strep- MYC deltaCTD, deltaMB0-1-His protein expression in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6159)
  TITLE     Myc-related plasmids
REFERENCE   2  (bases 1 to 6159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: M24
FEATURES             Location/Qualifiers
     source          1..6159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..28
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             803..829
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             905..922
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             933..950
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1003..1021)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1017..1064
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1101..1556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1188..1207)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1398..1419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(1648..2463)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1648..2463)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2371..2390
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2455..2474
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2585..3173
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3074..3093
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3327..3344
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3359..3498
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3424..3443)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3584..3606
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3600..3791)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(4600..5682)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5644..5663
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(5683..5760)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     5966..5985
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     6073..6092
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6073..6091
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6092..6116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6131..6153
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tatgtggtcc caccctcagt tcgagaagat gccgtcgcgt cgttccggac tttgtagtcc
       61 ttcctatgtc gccgtaaccc cgttttcctt gcgcggtgac aacgacggcg ggggaggtag
      121 cttttctact gcggatcaac ttgaaatggt cacggaactg ctggggggag atatggtaaa
      181 ccagtccttc atctgcgacc ctgatgacga gacatttatt aagaacatta tcatccagga
      241 ttgcatgtgg tcaggctttt cggcagccgc caaactggtc agcgagaaat tggcatctta
      301 tcaggcagca cgtaaggatt ctggaagccc caatcctgcg cgtgggcact cagtctgttc
      361 gacgtccagc ttgtacttgc aagatctgag cgccgctgct agcgaatgta ttgacccaag
      421 cgttgtattc ccttatccac tgaacgatag ttctagcccc aaatcttgcg cctcccaaga
      481 ttcatcagcc ttctcgccga gtagcgatag ccttttaagc tctacagagt cgagtccgca
      541 gggatcaccg gaacctttag tattacatga ggaaaccccg cctactacct catctgattc
      601 ggaggaggag caggaggatg aggaagaaat tgacgtagtc tctgtcgaaa aacgccaagc
      661 tccgggcaag cgcagtgagt cagggagccc ctcggcaggt gggcatagta agccgccaca
      721 tagtccactt gtgctgaagc gctgccacgt ctccacccat cagcataatt atgccgcccc
      781 tcccagcacc cgtaaggatt atccagcagc gaaacgcgtc aaattagact cggtacgcgt
      841 gctgcgccag atcagcaaca atcgcaagtg tacttcgcca cgctcgtcag atacggaaga
      901 gaaccaccac catcatcacc actaacctcg agcaccacca ccaccaccac tgagatccgg
      961 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
     1021 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
     1081 tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     1141 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     1201 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     1261 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     1321 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     1381 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     1441 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     1501 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     1561 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     1621 aatatgtatc cgctcatgaa ttaattctta gaaaaactca tcgagcatca aatgaaactg
     1681 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
     1741 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
     1801 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
     1861 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat
     1921 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
     1981 aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt
     2041 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
     2101 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg
     2161 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
     2221 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc
     2281 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg
     2341 atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
     2401 agcatccatg ttggaattta atcgcggcct agagcaagac gtttcccgtt gaatatggct
     2461 cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc atgaccaaaa
     2521 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2581 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2641 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2701 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     2761 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2821 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2881 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2941 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3001 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3061 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3121 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3181 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3241 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3301 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3361 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct
     3421 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     3481 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     3541 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     3601 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg
     3661 tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc
     3721 tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc
     3781 tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg
     3841 atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg
     3901 gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact
     3961 cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag
     4021 catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga
     4081 ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg
     4141 cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg
     4201 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggg
     4261 gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg
     4321 acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc
     4381 gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt
     4441 cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc
     4501 gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt
     4561 gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg
     4621 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     4681 cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc
     4741 cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag
     4801 gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc
     4861 gtcgtatccc actaccgaga tgtccgcacc aacgcgcagc ccggactcgg taatggcgcg
     4921 cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc
     4981 attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc
     5041 cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg
     5101 cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac
     5161 cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg
     5221 tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc
     5281 aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag
     5341 aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac
     5401 cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc
     5461 gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg
     5521 ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg
     5581 cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac
     5641 accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg
     5701 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc
     5761 cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
     5821 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
     5881 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     5941 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     6001 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atcgatctcg
     6061 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc
     6121 tagaaataat tttgtttaac tttaagaagg agatataca
//
