LOCUS       sequence-486723-        5821 bp    DNA     circular SYN 19-SEP-2025
DEFINITION  Expresses VSVGmut protein in mammalian cells for lentiviral 
            pseudotyping.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Dobson CS, Reich AN, Gaglione S, Smith BE, Kim EJ, Dong J, Ronsard 
            L, Okonkwo V, Lingwood D, Dougan M, Dougan SK, Birnbaum ME
  TITLE     Antigen identification and high-throughput interaction mapping by 
            reprogramming viral entry.
  JOURNAL   Nat Methods. 2022 Apr;19(4):449-460. doi: 
            10.1038/s41592-022-01436-z. Epub 2022 Apr 8.
  PUBMED    35396484
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-022-01436-z"; journalName: "Nat Methods"; date: 
            "2022-04"; volume: "19"; issue: "4"; pages: "449-460"
COMMENT     Sequence Label: pMD2-VSVGmut
FEATURES             Location/Qualifiers
     source          1..5821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..59)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        161..540
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        541..744
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     695..715
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     741..765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          878..1353
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             join(1428..1475,1476..2963)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPQSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTEAELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    3163..3557
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(3896..3913)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4067..4655)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4147..4166)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4826..5617,5618..5686))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5449..5468
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5687..5791)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatcccctg agggggcccc catgggctag aggatccggc ctcggcctct gcataaataa
       61 aaaaaattag tcagccatga gcttggccca ttgcatacgt tgtatccata tcataatatg
      121 tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt
      181 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      241 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      301 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      361 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      421 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      481 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      541 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      601 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      661 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      721 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      781 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt
      841 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg
      901 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag
      961 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct
     1021 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
     1081 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
     1141 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
     1201 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
     1261 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
     1321 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
     1381 ctggcccatc actttggcaa agcacgtgag atctgaattc tgacactatg aagtgccttt
     1441 tgtacttagc ctttttattc attggggtga attgcaagtt caccatagtt tttccacaca
     1501 accaaaaagg aaactggaaa aatgttcctt ctaattacca ttattgcccg tcaagctcag
     1561 atttaaattg gcataatgac ttaataggca cagccttaca agtcaaaatg ccccagagtc
     1621 acaaggctat tcaagcagac ggttggatgt gtcatgcttc caaatgggtc actacttgtg
     1681 atttccgctg gtatggaccg aagtatataa cacattccat ccgatccttc actccatctg
     1741 tagaacaatg caaggaaagc attgaacaaa cgaaacaagg aacttggctg aatccaggct
     1801 tccctcctca aagttgtgga tatgcaactg tgacggatgc cgaagcagtg attgtccagg
     1861 tgactcctca ccatgtgctg gttgatgaat acacaggaga atgggttgat tcacagttca
     1921 tcaacggaaa atgcagcaat tacatatgcc ccactgtcca taactctaca acctggcatt
     1981 ctgactataa ggtcaaaggg ctatgtgatt ctaacctcat ttccatggac atcaccttct
     2041 tctcagagga cggagagcta tcatccctgg gaaaggaggg cacagggttc agaagtaact
     2101 actttgctta tgaaactgga ggcaaggcct gcaaaatgca atactgcaag cattggggag
     2161 tcagactccc atcaggtgtc tggttcgaga tggctgataa ggatctcttt gctgcagcca
     2221 gattccctga atgcccagaa gggtcaagta tctctgctcc atctcagacc tcagtggatg
     2281 taagtctaat tcaggacgtt gagaggatct tggattattc cctctgccaa gaaacctgga
     2341 gcaaaatcag agcgggtctt ccaatctctc cagtggatct cagctatctt gctcctaaaa
     2401 acccaggaac cggtcctgct ttcaccataa tcaatggtac cctaaaatac tttgagacca
     2461 gatacatcag agtcgatatt gctgctccaa tcctctcaag aatggtcgga atgatcagtg
     2521 gaactaccac agaagccgaa ctgtgggatg actgggcacc atatgaagac gtggaaattg
     2581 gacccaatgg agttctgagg accagttcag gatataagtt tcctttatac atgattggac
     2641 atggtatgtt ggactccgat cttcatctta gctcaaaggc tcaggtgttc gaacatcctc
     2701 acattcaaga cgctgcttcg caacttcctg atgatgagag tttatttttt ggtgatactg
     2761 ggctatccaa aaatccaatc gagcttgtag aaggttggtt cagtagttgg aaaagctcta
     2821 ttgcctcttt tttctttatc atagggttaa tcattggact attcttggtt ctccgagttg
     2881 gtatccatct ttgcattaaa ttaaagcaca ccaagaaaag acagatttat acagacatag
     2941 agatgaaccg acttggaaag taactcaaat cctgcacaac agattcttca tgtttggacc
     3001 aaatcaactt gtgataccat gctcaaagag gcctcaatta tatttgagtt tttaattttt
     3061 atgaaaaaaa aaaaaaaaac ggaattcacc ccaccagtgc aggctgccta tcagaaagtg
     3121 gtggctggtg tggctaatgc cctggcccac aagtatcact aagctcgctt tcttgctgtc
     3181 caatttctat taaaggttcc tttgttccct aagtccaact actaaactgg gggatattat
     3241 gaagggcctt gagcatctgg attctgccta ataaaaaaca tttattttca ttgcaatgat
     3301 gtatttaaat tatttctgaa tattttacta aaaagggaat gtgggaggtc agtgcattta
     3361 aaacataaag aaatgaagag ctagttcaaa ccttgggaaa atacactata tcttaaactc
     3421 catgaaagaa ggtgaggctg caaacagcta atgcacattg gcaacagccc ctgatgccta
     3481 tgccttattc atccctcaga aaaggattca agtagaggct tgatttggag gttaaagttt
     3541 tgctatgctg tattttacat tacttattgt tttagctgtc ctcatgaatg tcttttcact
     3601 acccatttgc ttatcctgca tctctcagcc ttgactccac tcagttctct tgcttagaga
     3661 taccaccttt cccctgaagt gttccttcca tgttttacgg cgagatggtt tctcctcgcc
     3721 tggccactca gccttagttg tctctgttgt cttatagagg tctacttgaa gaaggaaaaa
     3781 cagggggcat ggtttgactg tcctgtgagc ccttcttccc tgcctccccc actcacagtg
     3841 acccggaatc cctcgacatg gcagtctagc actagtgcgg ccgcagatct gcttcctcgc
     3901 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3961 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4021 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4081 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4141 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4201 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4261 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4321 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4381 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4441 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4501 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4561 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4621 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4681 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4741 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     4801 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4861 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4921 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4981 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     5041 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5101 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5161 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     5221 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5281 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     5341 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     5401 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     5461 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5521 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5581 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5641 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5701 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5761 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     5821 t
//
