LOCUS       sequence-486814-       10480 bp    DNA     circular SYN 22-SEP-2025
DEFINITION  Expresses flp recombinase in bacteria upon arabinose induction. No 
            flp activity is detected without induction..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10480)
  AUTHORS   Scholz SA, Diao R, Wolfe MB, Fivenson EM, Lin XN, Freddolino PL
  TITLE     High-Resolution Mapping of the Escherichia coli Chromosome Reveals 
            Positions of High and Low Transcription.
  JOURNAL   Cell Syst. 2019 Mar 27;8(3):212-225.e9. doi: 
            10.1016/j.cels.2019.02.004. Epub 2019 Mar 20.
  PUBMED    30904377
REFERENCE   2  (bases 1 to 10480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 22, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2019.02.004"; journalName: "Cell Syst"; date: 
            "2019-03-27- 27"; volume: "8"; issue: "3"; pages: "212-225.e9"
COMMENT     Sequence Label: pBAD-Flp
FEATURES             Location/Qualifiers
     source          1..10480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1083..1102)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1167..1186)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1693..2406)
                     /codon_start=1
                     /gene="cIts"
                     /product="temperature-sensitive variant of the phage lambda
                     repressor"
                     /label=lambda repressor (ts)
                     /note="thermosensitivity is conferred by the A67T mutation"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     CDS             complement(2448..3326)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRH
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3353..3637
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3560..3579
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             3655..4926
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     primer_bind     complement(5874..5892)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5960..6064
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6065..6133,6134..6925)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6283..6302)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(8387..9337)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      9385..9607
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     promoter        10159..10261
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             join(10262..10480,1..441)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(10328..10347)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
       61 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      121 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
      181 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
      241 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
      301 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
      361 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
      421 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
      481 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgaa agcaaattcg
      541 acccggtcgt cggttcaggg cagggtcgtt aaatagccgc ttatgtctat tgctggttta
      601 ccggtttatt gactaccgga agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt
      661 ttgctcaggc tctccccgtg gaggtaataa ttgacgatat gatcatttat tctgcctccc
      721 agagcctgat aaaaacggtt agcgccagca accgcacctg tggcgctgag gtctgcctcg
      781 tgaagaaggt gttgctgact cataccaggc ctgaatcgcc ccatcatcca gccagaaagt
      841 gagggagcca cggttgatga gagctttgtt gtaggtggac cagttggtga ttttgaactt
      901 ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc
      961 agcaaaagtt cgatttattc aacaaagcca cgttgtgtct caaaatctct gatgttacat
     1021 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     1081 tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctcgaggc cgcgattaaa
     1141 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     1201 aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     1261 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     1321 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     1381 actcaccact gcgatccccg ggaaaacagc attccaggta ttagaagaat atcctgattc
     1441 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     1501 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     1561 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     1621 acaagtctgg aaagaaatgc agattcttgc tcaattgtta tcagctatgc gccgaccaga
     1681 acaccttgcc gatcagccaa acgtctcttc aggccactga ctagcgataa ctttccccac
     1741 aacggaacaa ctctcattgc atgggatcat tgggtactgt gggtttagtg gttgtaaaaa
     1801 cacctgaccg ctatccctga tcagtttctt gaaggtaaac tcatcacccc caagtctggc
     1861 tatgcagaaa tcacctggct caacagcctg ctcagggtca acgagaatta acattccgtc
     1921 aggaaagctt ggcttggagc ctgttggtgc ggtcatggaa ttaccttcaa cctcaagcca
     1981 gaatgcagaa tcactggctt ttttggttgt gcttacccat ctctccgcat cacctttggt
     2041 aaaggttcta agcttaggtg agaacatccc tgcctgaaca tgagaaaaaa cagggtactc
     2101 atactcactt ctaagtgacg gctgcatact aaccgcttca tacatctcgt agatttctct
     2161 ggcgattgaa gggctaaatt cttcaacgct aactttgaga atttttgtaa gcaatgcggc
     2221 gttataagca tttaatgcat tgatgccatt aaataaagca ccaacgcctg actgccccat
     2281 ccccatcttg tctgcgacag attcctggga taagccaagt tcatttttct ttttttcata
     2341 aattgcttta aggcgacgtg cgtcctcaag ctgctcttgt gttaatggtt tcttttttgt
     2401 gctcatacgt taaatctatc accgcaaggg ataaatatct aacaccgtta tgacaacttg
     2461 acggctacat cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg
     2521 gtgcattttt taaatacccg cgagaagtag agttgatcgt caaaaccaac attgcgaccg
     2581 acggtggcga taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg
     2641 tcctcgcgcc agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc
     2701 gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg
     2761 tgatcgctga tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac
     2821 tcgttaatcg cttccatgtg ccgcagtaac aattgctcaa gcagatttat cgccagcagc
     2881 tccgaatagc gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa
     2941 tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag
     3001 ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg
     3061 cgagcctccg gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca
     3121 cccggtcggc aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg
     3181 agattgagaa tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg
     3241 ttggcctcaa tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc
     3301 aggggatcat tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc
     3361 aattgtccat attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta
     3421 accaaaccgg taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg
     3481 acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg
     3541 cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc
     3601 tgacgctttt tatcgcaact ctctactgtt tctccatacc cgttaggagg ttgtatgcca
     3661 caatttgata tattatgtaa aacaccacct aaggtgcttg ttcgtcagtt tgtggaaagg
     3721 tttgaaagac cttcaggtga gaaaatagca ttatgtgctg ctgaactaac ctatttatgt
     3781 tggatgatta cacataacgg aacagcaatc aagagagcca cattcatgag ctataatact
     3841 atcataagca attcgctgag tttcgatatt gtcaataaat cactccagtt taaatacaag
     3901 acgcaaaaag caacaattct ggaagcctca ttaaagaaat tgattcctgc ttgggaattt
     3961 acaattattc cttactatgg acaaaaacat caatctgata tcactgatat tgtaagtagt
     4021 ttgcaattac agttcgaatc atcggaagaa gcagataagg gaaatagcca cagtaaaaaa
     4081 atgcttaaag cacttctaag tgagggtgaa agcatctggg agatcactga gaaaatacta
     4141 aattcgtttg agtatacttc gagatttaca aaaacaaaaa ctttatacca attcctcttc
     4201 ctagctactt tcatcaattg tggaagattc agcgatatta agaacgttga tccgaaatca
     4261 tttaaattag tccaaaataa gtatctggga gtaataatcc agtgtttagt gacagagaca
     4321 aagacaagcg ttagtaggca catatacttc tttagcgcaa ggggtaggat cgatccactt
     4381 gtatatttgg atgaattttt gaggaattct gaaccagtcc taaaacgagt aaataggacc
     4441 ggcaattctt caagcaataa acaggaatac caattattaa aagataactt agtcagatcg
     4501 tacaataaag ctttgaagaa aaatgcgcct tattcaatct ttgctataaa aaatggccca
     4561 aaatctcaca ttggaagaca tttgatgacc tcatttcttt caatgaaggg cctaacggag
     4621 ttgactaatg ttgtgggaaa ttggagcgat aagcgtgctt ctgccgtggc caggacaacg
     4681 tatactcatc agataacagc aatacctgat cactacttcg cactagtttc tcggtactat
     4741 gcatatgatc caatatcaaa ggaaatgata gcattgaagg atgagactaa tccaattgag
     4801 gagtggcagc atatagaaca gctaaagggt agtgctgaag gaagcatacg ataccccgca
     4861 tggaatggga taatatcaca ggaggtacta gactaccttt catcctacat aaatagacgc
     4921 atataagtac gcatttaagc ataaacacgc actatgccgt tcttctcatg tatatatata
     4981 tacaggcaac acgcagatat aggtgcgacg tgaacagtga gctgtatgtg cgcagctcgc
     5041 gttgcatttt cggaagcgct cgttttcgga aacgctttga agttcctatt ccgaagttcc
     5101 tattctgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     5161 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     5221 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     5281 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     5341 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     5401 cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt tgctgagttg
     5461 aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc
     5521 aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc
     5581 tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc ttcacgaggc
     5641 agacctcagc gccggtgatg ccggccacga tgcgtccggc gtagaggatc ctagagcgca
     5701 cgaatgaggg ccgacaggaa gcaaagctga aaggaatcaa atttggccgc aggcgtaccg
     5761 tggacaggaa cgtcgtgctg acgcttcatc agaagggcac tggtgcaacg gaaattgctc
     5821 atcagctcag tattgcccgc tccacggttt ataaaattct tgaagacgaa agggcctcgt
     5881 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     5941 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6001 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6061 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6121 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6181 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6241 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6301 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6361 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6421 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6481 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6541 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6601 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6661 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6721 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6781 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6841 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6901 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6961 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7021 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtttagtc
     7081 actgatgcct ccgtgtaagg gggatttctg ttcatggggt aatgataccg atgaaacgcg
     7141 agaggatgct cacaatacgg gttactgatg atgaacatgc ccggttactt gaacgctgtg
     7201 agggtaaaca actggcggta tggatgcggc gggtctgcct gggggagccg gttgcccgtt
     7261 ccggaaaact gccgacactg gcaccgccgt tactgcgtca gctggccgcc atcggaaata
     7321 acctgaatca gacagcccgt aaggtgaaca gcgggcagtg gtcttccggt gaccgggttc
     7381 aggtggtggc cgcactgatg gccatcgggg atgagctgcg ccggctgcgt ctggctgtca
     7441 gggaacaggg ggcgcgggat gatagttaaa tttcatgcca ggggaaaagg tggtggcagt
     7501 ggtccggttg attacctgct ggggagggag cgtaaccgcg aaggcgcaac ggtgcttcag
     7561 ggtaatccgg aagaagtccg ggaactcatc gatgccacgc catttgcgaa gaaatacacg
     7621 tccggtgttc tgtcgttcgc ggagaaggag ctgccgccgg gaggacgtga aaaagtgatg
     7681 gcgagctttg agcgtgtact gatgcccggt ctcgaaaaaa atcagtacag catcctgtgg
     7741 gtggagcacc aggacaaggg acggcttgag ctgaattttg tcattccgaa catggagcta
     7801 cagaccggaa aacgcctcca gccgtactac gaccgcgcag acaggcctag aattgatgcc
     7861 tggcagacgc tggtaaatca ccattacggg ctgcatgacc cgaacgcccc ggagaaccgc
     7921 aggacgctga cactccctga taacctgcct gaaacgaaac aggcgctatt tcttccagaa
     7981 ttgccatgat tttttcccca cgggaggcgt cactggctcc cgtgttgtcg gcagctttga
     8041 ttcgataagc agcatcgcct gtttcaggct gtctatgtgt gactgttgag ctgtaacaag
     8101 ttgtctcagg tgttcaattt catgttctag ttgctttgtt ttactggttt cacctgttct
     8161 attaggtgtt acatgctgtt catctgttac attgtcgatc tgttcatggt gaacagcttt
     8221 aaatgcacca aaaactcgta aaagctctga tgtatctatc ttttttacac cgttttcatc
     8281 tgtgcatatg gacagttttc cctttgatat gtaacggtga acagttgttc tacttttgtt
     8341 tgttagtctt gatgcttcac tgatagatac aagagccata agaacctcag atccttccgt
     8401 atttagccag tatgttctct agtgtggttc gttgtttttg cgtgagccat gagaacgaac
     8461 cattgagatc atacttactt tgcatgtcac tcaaaaattt tgcctcaaaa ctggtgagct
     8521 gaatttttgc agttaaagca tcgtgtagtg tttttcttag tccgttatgt aggtaggaat
     8581 ctgatgtaat ggttgttggt attttgtcac cattcatttt tatctggttg ttctcaagtt
     8641 cggttacgag atccatttgt ctatctagtt caacttggaa aatcaacgta tcagtcgggc
     8701 ggcctcgctt atcaaccacc aatttcatat tgctgtaagt gtttaaatct ttacttattg
     8761 gtttcaaaac ccattggtta agccttttaa actcatggta gttattttca agcattaaca
     8821 tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt gtgagttttc ttttgtgtta
     8881 gttcttttaa taaccactca taaatcctca tagagtattt gttttcaaaa gacttaacat
     8941 gttccagatt atattttatg aattttttta actggaaaag ataaggcaat atctcttcac
     9001 taaaaactaa ttctaatttt tcgcttgaga acttggcata gtttgtccac tggaaaatct
     9061 caaagccttt aaccaaagga ttcctgattt ccacagttct cgtcatcagc tctctggttg
     9121 ctttagctaa tacaccataa gcattttccc tactgatgtt catcatctga acgtattggt
     9181 tataagtgaa cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg gggttgagta
     9241 gtgccacaca gcataaaatt agcttggttt catgctccgt taagtcatag cgactaatcg
     9301 ctagttcatt tgctttgaaa acaactaatt cagacataca tctcaattgg tctaggtgat
     9361 tttaatcact ataccaattg agatgggcta gtcaatgata attactagtc cttttccttt
     9421 gagttgtggg tatctgtaaa ttctgctaga cctttgctgg aaaacttgta aattctgcta
     9481 gaccctctgt aaattccgct agacctttgt gtgttttttt tgtttatatt caagtggtta
     9541 taatttatag aataaagaaa gaataaaaaa agataaaaag aatagatccc agccctgtgt
     9601 ataactcact actttagtca gttccgcagt attacaaaag gatgtcgcaa acgctgtttg
     9661 ctcctctaca aaacagacct taaaacccta aaggcttaag tagcaccctc gcaagctcgg
     9721 gcaaatcgct gaatattcct tttgtctccg accatcaggc acctgagtcg ctgtcttttt
     9781 cgtgacattc agttcgctgc gctcacggct ctggcagtga atgggggtaa atggcactac
     9841 aggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
     9901 tgatgtccgg cggtgctttt gccgttacgc accaccccgt cagtagctga acaggaggga
     9961 cagctgatag aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag
    10021 taagttggca gcatcacccg acgcactttg cgccgaataa atacctgtga cggaagatca
    10081 cttcgcagaa taaataaatc ctggtgtccc tgttgatacc gggaagccct gggccaactt
    10141 ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat
    10201 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa
    10261 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
    10321 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
    10381 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
    10441 tcacattctt gcccgcctga tgaatgctca tccggaattc
//
