LOCUS       sequence-486857-        5067 bp    DNA     circular SYN 22-SEP-2025
DEFINITION  Express NanoLuc(R)-CCDC6 Fusion Protein in Mammalian Cells under a 
            CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 22, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NanoLuc-CCDC6 Vector
FEATURES             Location/Qualifiers
     source          1..5067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..54
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     5..25
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          190..322
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     305..324
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     366..385
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        366..384
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             398..910
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(2467..2485)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    2522..2643
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2533..2552)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2587..2606
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2888..3245
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2888..3079
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2911..2931)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3096..3231
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3260..3307
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3339..4133
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3393..3412)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4003..4022
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4197..4245
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(4564..4583)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
       61 gtgaaccgtc agatcactag aagctttatt gcggtagttt atcacagtta aattgctaac
      121 gcagtcagtg cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc
      181 actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc
      241 ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc
      301 cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag
      361 agtattaata cgactcacta tagggctagc gctcaccatg gtcttcacac tcgaagattt
      421 cgttggggac tggcgacaga cagccggcta caacctggac caagtccttg aacagggagg
      481 tgtgtccagt ttgtttcaga atctcggggt gtccgtaact ccgatccaaa ggattgtcct
      541 gagcggtgaa aatgggctga agatcgacat ccatgtcatc atcccgtatg aaggtctgag
      601 cggcgaccaa atgggccaga tcgaaaaaat ttttaaggtg gtgtaccctg tggatgatca
      661 tcactttaag gtgatcctgc actatggcac actggtaatc gacggggtta cgccgaacat
      721 gatcgactat ttcggacggc cgtatgaagg catcgccgtg ttcgacggca aaaagatcac
      781 tgtaacaggg accctgtgga acggcaacaa aattatcgac gagcgcctga tcaaccccga
      841 cggctccctg ctgttccgag taaccatcaa cggagtgacc ggctggcggc tgtgcgaacg
      901 cattctggcg ggctcgagcg gcgcgatcgc catggcggac agcgccagcg agagcgacac
      961 ggacggggcg gggggcaaca gcagcagctc ggccgccatg cagtcgtcct gctcgtcgac
     1021 ctcgggcggc ggcggtggcg gcgggggagg cggcggcggt gggaagtcgg ggggcattgt
     1081 catctcgccg ttccgcctgg aggagctcac caaccgcctg gcctcgctgc agcaagagaa
     1141 caaggtgctg aagatagagc tggagaccta caaactgaag tgcaaggcac tgcaggagga
     1201 gaaccgcgac ctgcgcaaag ccagcgtgac catccaagcc agggctgagc aggaagaaga
     1261 attcattagt aacactttat tcaagaaaat tcaggctttg cagaaggaga aagaaaccct
     1321 tgctgtaaat tatgagaaag aagaagaatt cctcactaat gagctctcca gaaaattgat
     1381 gcagttgcag catgagaaag ccgaactaga acagcatctt gaacaagagc aggaatttca
     1441 ggtcaacaaa ctgatgaaga aaattaaaaa actggagaat gacaccattt ctaagcaact
     1501 tacattagaa cagttgagac gggagaagat tgaccttgaa aatacattgg aacaagaaca
     1561 agaagcacta gttaatcgcc tctggaaaag gatggataag cttgaagctg aaaagcgaat
     1621 cctgcaggaa aaattagacc agcccgtctc tgctccacca tcgcctagag atatctccat
     1681 ggagattgat tctccagaaa atatgatgcg tcacatcagg tttttaaaga atgaagtgga
     1741 acggctgaag aagcaactga gagctgctca gttacagcat tcagagaaaa tggcacagta
     1801 tctggaggag gaacgtcaca tgagagaaga gaacttgagg ctccagagga agctgcagag
     1861 ggagatggag agaagagaag ccctctgtcg acagctctcc gagagtgagt ccagcttaga
     1921 aatggacgac gaaaggtatt ttaatgagat gtctgcacaa ggattaagac ctcgcactgt
     1981 gtccagcccg atcccttaca caccttctcc gagttcaagc aggcctatat cacctggtct
     2041 atcatatgca agtcacacgg ttggtttcac gccaccaact tcactgacta gagctggaat
     2101 gtcttattac aattccccgg gtcttcacgt gcagcacatg ggaacatccc atggtatcac
     2161 aaggccttca ccacggagaa gcaacagtcc tgacaaattc aaacggccca cgccgcctcc
     2221 atctcccaac acacagaccc cagtccagcc acctccgcct ccacctccgc cacccatgca
     2281 gcccacggtc ccctcagcag ccacctcgca gcctactcct tcgcaacatt cggcgcaccc
     2341 ctcctcccag cctgtttaaa cgaattcggg ctcggtaccc ggggatcctc tagagtcgac
     2401 ctgcaggcat gcaagctgat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     2461 ctgccaccgc tgagcaataa ctagcataac cccttggggc ggccgcttcg agcagacatg
     2521 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     2581 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     2641 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt
     2701 tttttaagca agtaaaacct ctacaaatgt ggtaaaatcg aattttaaca aaatattaac
     2761 gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     2821 atacgcggat ctgcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag
     2881 gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     2941 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3001 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3061 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3121 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3181 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3241 gcaaaaagct taattaactg ttgacaatta atcatcggca tagtatatcg gcatagtata
     3301 atacgacaag gtgaggaact aaacccagga ggcagatcat gattgaacaa gatggattgc
     3361 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     3421 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     3481 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     3541 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     3601 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     3661 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     3721 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     3781 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     3841 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     3901 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     3961 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     4021 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     4081 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     4141 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgat ggccgcaata
     4201 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagcgataa
     4261 ggatcctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc
     4321 ggggtcagca ccgtttctgc ggactggctt tctacccggt atcagctcac tcaaaggcgg
     4381 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4441 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4501 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4561 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4621 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4681 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4741 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4801 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4861 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4921 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4981 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5041 agcagattac gcgcagaaaa aaaggat
//
