LOCUS       sequence-486869-        9505 bp    DNA     circular SYN 22-SEP-2025
DEFINITION  A CAGGS driven, Dre recombinase dependent switch reporter 
            (nls-mKate2 to MbBFP following Dre recombination). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9505)
  AUTHORS   Gillespie W, Zhang Y, Ruiz OE, Cerda J, Ortiz-Guzman J, Turner WD, 
            Largoza G, Sherman M, Mosser LE, Fujimoto E, Chien CB, Kwan KM, 
            Arenkiel BR, Devine WP, Wythe JD
  TITLE     Multisite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox with diverse applications in vertebrate model 
            systems.
  JOURNAL   bioRxiv [Preprint]. 2024 Jul 13:2024.07.13.603267. doi: 
            10.1101/2024.07.13.603267.
  PUBMED    39026881
REFERENCE   2  (bases 1 to 9505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 22, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jul 13:2024.07.13.603267. doi: 
            10.1101/2024.07.13.603267."
COMMENT     Sequence Label: pCAGGS-Rox-nlsKate5V-stop-Rox-myr-Flag-BFP-NEO...
FEATURES             Location/Qualifiers
     source          1..9505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        726..1029
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1031..1306
                     /label=chicken beta-actin promoter
     intron          1307..2323
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2247..2269
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2331..2350
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2430..2439
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2463..2483
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2487..2507
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2511..2531
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2547..3242
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             3243..3284
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    3452..3507
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     protein_bind    3901..3925
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3932..3941
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3962..4660
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    4661..4726
                     /label=MCS
                     /note="multiple cloning site"
     CDS             4739..4762
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(4774..4798)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    4955..5010
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     protein_bind    complement(5374..5407)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    5427..5888
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     CDS             complement(5898..6701)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6009..6028)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6619..6638
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6715..7214)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6763..6782)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7156..7179
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(7230..7263)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        complement(7317..7335)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7356..7372)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7356..7372)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7369..7391)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7380..7396
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7404..7410,7411..7428,7429..7434))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7449..7470
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7587..7604)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7758..8346)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7838..7857)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8517..9308,9309..9377))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTXFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9140..9159
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9378..9482)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccggcgc
      661 gccactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      721 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      781 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      841 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      901 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      961 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     1021 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
     1081 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
     1141 gggggggggg ggsgcgccrg gsggggsggg gcggggcgrg gggsggggcg gggcgaggcg
     1201 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     1261 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     1321 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     1381 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
     1441 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     1501 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
     1561 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
     1621 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
     1681 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
     1741 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
     1801 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
     1861 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
     1921 cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc
     1981 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt
     2041 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     2101 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
     2161 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
     2221 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     2281 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     2341 ggttattgtg ctgtctcatc attttggcaa agaattcaag ctttaacttt aaataatgcc
     2401 aattatttaa agttactagg ttcccgcggg ccaccatggc cacaaccatg gtgcacgtgg
     2461 atccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtagat ccaaaaaaga
     2521 agagaaaggt acacgtgtcc acgcgtatgg tgagcgagct gattaaggag aacatgcaca
     2581 tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca tccgagggcg
     2641 aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggcggtcgag ggcggccctc
     2701 tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaaa accttcatca
     2761 accacaccca gggcatcccc gacttcttta agcagtcctt ccccgagggc ttcacatggg
     2821 agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc
     2881 aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttccca tccaacggcc
     2941 ctgtgatgca gaagaaaaca ctcggctggg aggcctccac cgagaccctg taccccgctg
     3001 acggcggcct ggaaggcaga gccgacatgg ccctgaagct cgtgggcggg ggccacctga
     3061 tctgcaactt gaagaccaca tacagatcca agaaacccgc taagaacctc aagatgcccg
     3121 gcgtctacta tgtggacaga agactggaaa gaatcaagga ggccgacaaa gagacctacg
     3181 tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca
     3241 gaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagccg cgggaaccta
     3301 ggcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     3361 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
     3421 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     3481 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     3541 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
     3601 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     3661 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     3721 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     3781 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
     3841 ctgcagctag taactttaaa taatgccaat tatttaaagt tactaggatt cgaggatatc
     3901 acaagtttgt acaaaaaagc aggctttaaa cgccaccatg ggttgctgtt tctccaagac
     3961 catgagcgag ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga
     4021 caaccatcac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
     4081 catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac
     4141 tagcttcctc tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt
     4201 caagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg
     4261 cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa
     4321 gatcagaggg gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg
     4381 ggaggccttc accgagacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat
     4441 ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac atcaagacca catatagatc
     4501 caagaaaccc gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga
     4561 aagaatcaag gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag
     4621 atactgcgac ctccctagca aactgggaca caagcttaat tccggactca gatctcgagc
     4681 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagaga
     4741 ctacaaagac gatgacgaca agtagccatc tagacccagc tttcttgtac aaagtggtga
     4801 tatcgcggcc gcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
     4861 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
     4921 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     4981 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     5041 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     5101 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     5161 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     5221 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     5281 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
     5341 gacctgcagc ccaagctatc ctagattttc gacgaagttc ctatactttc tagagaatag
     5401 gaacttcctc gacggtatcg ataagcttct gatggaatta gaacttggca aaacaatact
     5461 gagaatgaag tgtatgtgga acagaggctg ctgatctcgt tcttcaggct atgaaactga
     5521 cacatttgga aaccacagta cttagaacca caaagtggga atcaagagaa aaacaatgat
     5581 cccacgagag atctatagat ctatagatca tgagtgggag gaatgagctg gcccttaatt
     5641 tggttttgct tgtttaaatt atgatatcca actatgaaac attatcataa agcaatagta
     5701 aagagccttc agtaaagagc aggcatttat ctaatcccac cccaccccca cccccgtagc
     5761 tccaatcctt ccattcaaaa tgtaggtact ctgttctcac ccttcttaac aaagtatgac
     5821 aggaaaaact tccattttag tggacatctt tattgtttaa tagatcatca atttctgcag
     5881 acttacagcg gatcccctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     5941 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     6001 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
     6061 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     6121 gcatcgccat gggtcacgac gagatcatcg ccgtcgggca tgcgcgcctt gagcctggcg
     6181 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     6241 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     6301 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     6361 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     6421 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     6481 gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg
     6541 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     6601 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     6661 gaacctgcgt gcaatccatc ttgttcaatg gccgatccca tattggctgc aggtcgaaag
     6721 gcccggagat gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa
     6781 tgccgggcct ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc
     6841 gcccccggac ccaccccttc ccagcctctg agcccagaaa gcgaaggagc aaagctgcta
     6901 ttggccgctg ccccaaaggc ctacccgctt ccattgctca gcggtgctgt ccatctgcac
     6961 gagactagtg agacgtgcta cttccatttg tcacgtcctg cacgacgcga gctgcggggc
     7021 gggggggaac ttcctgacta ggggaggagt agaaggtggc gcgaaggggc caccaaagaa
     7081 cggagccggt tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt
     7141 gtagcgccaa gtgcccagcg gggctgctaa agcgcatgct ccagactgcc ttgggaaaag
     7201 cgcctcccct acccggtaga attggccgcg aagttcctat actttctaga gaataggaac
     7261 ttcggccgcc accgcggtgg aaatcccggg ttaattaaga gctccagctt ttgttccctt
     7321 tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7381 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7441 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7501 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7561 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7621 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7681 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7741 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7801 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7861 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7921 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7981 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8041 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8101 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8161 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8221 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8281 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8341 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8401 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8461 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8521 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8581 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8641 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8701 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8761 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8821 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8881 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagyggtt
     8941 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9001 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9061 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9121 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9181 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9241 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9301 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9361 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9421 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9481 cgcacatttc cccgaaaagt gccac
//
