LOCUS       sequence-486982-         940 bp    DNA     linear   UNA 23-SEP-2025
DEFINITION  HA-tagged human RhoA(G14V) cloned into the pCW backbone (Addgene 
            #41393, with puromycin resistance) together with an IRES GFP to 
            indicate doxycycline-inducible expression by fluorescence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  TITLE     Doxycycline-inducible pCW vectors with HA-tagged genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCW-Rho(G14V)-HA
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8..586
                     /codon_start=1
                     /product="human Rho family small GTPase RhoA"
                     /label=RhoA
                     /translation="MAAIRKKLVIVGDVACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     CDS             590..616
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             620..646
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(875..892)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 cgccaccatg gctgccatcc ggaagaaact ggtgattgtt ggtgatgtag cctgtggaaa
       61 gacatgcttg ctcatagtct tcagcaagga ccagttccca gaggtgtatg tgcccacagt
      121 gtttgagaac tatgtggcag atatcgaggt ggatggaaag caggtagagt tggctttgtg
      181 ggacacagct gggcaggaag attatgatcg cctgaggccc ctctcctacc cagataccga
      241 tgttatactg atgtgttttt ccatcgacag ccctgatagt ttagaaaaca tcccagaaaa
      301 gtggacccca gaagtcaagc atttctgtcc caacgtgccc atcatcctgg ttgggaataa
      361 gaaggatctt cggaatgatg agcacacaag gcgggagcta gccaagatga agcaggagcc
      421 ggtgaaacct gaagaaggca gagatatggc aaacaggatt ggcgcttttg ggtacatgga
      481 gtgttcagca aagaccaaag atggagtgag agaggttttt gaaatggcta cgagagctgc
      541 tctgcaagct agacgtggga agaaaaaatc tgggtgcctt gtcttgggat acccatacga
      601 tgttcctgac tatgcgggct atccctatga cgtcccggac tatgcagggc cggctaaggc
      661 agcggcacgg gcggtgtagc tcgagggccg gcgcgccgcg gccgctacgt aaattccgcc
      721 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
      781 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
      841 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
      901 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
//
