LOCUS       sequence-487012-       24812 bp    DNA     circular SYN 23-SEP-2025
DEFINITION  PVC structural operon - tail fiber (Pvc13) tagged with SpyTag - 
            deleted spike tip (Pvc10).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 24812)
  TITLE     Feng Zhang Lab - PVC plasmids
REFERENCE   2  (bases 1 to 24812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAWP78-PVCpnf_pvc13-SpyTag_Delta-pvc10
FEATURES             Location/Qualifiers
     source          1..24812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(339..1154)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(339..1154)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1062..1081
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1146..1165
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(1458..2606)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2878..3249)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            3282..3391
                     /note="incP origin of transfer"
     rep_origin      3544..4175
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      4484..5072
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4973..4992
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagatgatga taagaatggt cagtcaccag ataaaatgat ctctggtcaa ttttaggaaa
       61 gctgaaaaaa cctgaccaaa tgcgtcgtaa attctatgct tagaaagact cggtgccagt
      121 gtaataccta agatatggtt tttatttcca tatctgttcg aaaacttaga aataacaagt
      181 tcaacaataa ccagggactt taaatatcgg atccttgtcg ggaagatgcg tgatctgatc
      241 cttcaactca gcaaaagttc gatttattca acaaagccgc cgtcccgtca agtcagcgta
      301 atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
      361 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
      421 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
      481 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
      541 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
      601 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
      661 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
      721 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
      781 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
      841 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
      901 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
      961 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     1021 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     1081 ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt
     1141 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     1201 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     1261 ctttcgactt ccggcaagct atacgcgccc tagaattgtc aattttaatc ctctgtttat
     1321 cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga
     1381 gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg ctgctgaacc cccagccgga
     1441 actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt
     1501 tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc
     1561 ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac
     1621 ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg
     1681 tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg
     1741 tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc
     1801 agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc
     1861 tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc
     1921 aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc
     1981 gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg
     2041 gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg
     2101 cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc
     2161 gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc
     2221 tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg
     2281 tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc
     2341 gacttcgcca aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat
     2401 ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct
     2461 tgctggacca tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg
     2521 cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat
     2581 gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc
     2641 ggggcaatgt gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc
     2701 accttatcgg caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta
     2761 ttccgaatct tgccctgcac gaataccagc tccgcgaagt cgctcttctt gatggagcgc
     2821 atggggacgt gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca
     2881 tggctctgcc ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc
     2941 ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc
     3001 gtcggtgagc cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc
     3061 cagctcgcgg acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc
     3121 cagcaggtag gcctacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg
     3181 ttcgtctgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc
     3241 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
     3301 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
     3361 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
     3421 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
     3481 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagatcc gtcgaccctt
     3541 tccgacgctc accgggctgg ttgccctcgc cgctgggctg gcggccgtct atggccctgc
     3601 aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cgcggccgcc ggcgttgtgg
     3661 atacctcgcg gaaaacttgg ccctcactga cagatgaggg gcggacgttg acacttgagg
     3721 ggccgactca cccggcgcgg cgttgacaga tgaggggcag gctcgatttc ggccggcgac
     3781 gtggagctgg ccagcctcgc aaatcggcga aaacgcctga ttttacgcga gtttcccaca
     3841 gatgatgtgg acaagcctgg ggataagtgc cctgcggtat tgacacttga ggggcgcgac
     3901 tactgacaga tgaggggcgc gatccttgac acttgagggg cagagtgctg acagatgagg
     3961 ggcgcaccta ttgacatttg aggggctgtc cacaggcaga aaatccagca tttgcaaggg
     4021 tttccgcccg tttttcggcc accgctaacc tgtcttttaa cctgctttta aaccaatatt
     4081 tataaacctt gtttttaacc agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg
     4141 gggtgccccc ccttctcgaa ccctcccggc ccgctaacgc gggcctccca tccccccagg
     4201 ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat ggcagccaag ctgacccgct
     4261 agggacgtga agtcgattcc ttcgatggtt agcaatcaaa gactcagagt gctgtctgga
     4321 gcgtgaatct aacggtacgt atctcgattg ctcggtcgct attcgcactc tgcgaaagtt
     4381 cgtaccgctc attcactagg ttgcgaatca tgaccaaaat cccttaacgt gagttttcgt
     4441 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4501 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4561 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4621 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4681 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4741 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4801 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4861 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4921 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4981 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5041 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5101 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5161 tggataaccg tattaccgcc tttgagtgag ctgtcgcgaa tgcatctaga tataattaca
     5221 tcttcatcat tcggtatttt aaatgacata tatcctcctt ccgaatagtt ttaatggtga
     5281 tgttcgattg gacagtgttt tccattatta aaattttaag tatcacatca tgtttaatga
     5341 aggattgatg tttattagaa tattcataat agctttgtct ttgcctctat gtaaatgaaa
     5401 ttgcataaaa agcaaattgc attattggca atgtacttat tctggatttt tcggcacgat
     5461 aaattattgg tgtaagtttt atatcatgag taaaaattcg gaatgatatt cctgtcattt
     5521 tgtggcggta gcgttactca agatattatc ctacatcata tgttgattat tgtaatcaat
     5581 cgagctattt tttgtctaaa aaggggcttt tatgtctaca agtacatctc aaattgcggt
     5641 tgaatatcct attcctgtct atcgctttat tgtttctgtc ggagatgaga aaattccatt
     5701 taatagtgtt tcaggattag atattagtta tgacaccatt gaataccgag atggtgttgg
     5761 taattggttc aaaatgccgg gtcagagtca gagcactaat atcaccttgc gtaaaggcgt
     5821 tttcccgggg aaaacagaac tgtttgattg gattaactct attcagctta atcaggtaga
     5881 gaaaaaggat attaccatca gtttaactaa tgatgcaggt accgaattat taatgacctg
     5941 gaatgtttct aatgcttttc ccacttcatt gacttcacct tcatttgatg ccaccagtaa
     6001 tgatattgca gtacaggaaa ttacgctgat ggcagatcgg gtgattatgc aggctgtttg
     6061 aagcattgat atttaatcat ctcatataag ggaactttta tgacaaccgt taccagttat
     6121 cctggcgttt atattgaaga attaaatagc ctggccttgt cagtttcaaa tagcgccaca
     6181 gcggttcctg tttttgctgt ggacgaacaa aaccaatata ttagtgaaga taatgcaatc
     6241 cgtattaatt cgtggatgga ttatcttaat ctgattggca attttaataa tgaagacaaa
     6301 ttagatgttt ctgtgcgtgc ttattttgcc aatggaggtg gatattgtta tctcgtcaaa
     6361 acaacgagtt tagaaaaaat tattccaacc ttggatgatg taaccttatt ggttgctgcg
     6421 ggcgaagata ttaaaacgac agtagatgtt ttatgtcagc caggaaaagg gttattcgca
     6481 gtctttgatg gccctgaaac agagttgact atcaacggtg cggaagaggc aaaacaagcc
     6541 tataccgcca caccattcgc tgcggtttat tatccttggt tgaaagcgga ttgggctaac
     6601 atagatattc cacccagtgc agtgatggcg ggagtttatg catcggtgga tttatcccgt
     6661 ggtgtatgga aagcgcctgc caatgttgcg ttgaaagggg gcctggaacc taaattttta
     6721 gtcacggatg aattgcaggg tgaatataac actggccgcg ctatcaatat gattcgtaat
     6781 ttcagtaaca caggtactac ggtttggggt gcaagaaccc tggaagataa agacaattgg
     6841 cgttatgttc cagtgcgacg cttgtttaat tctgtggagc gggatatcaa gcgtgccatg
     6901 agctttgcta tgttcgagcc taataatcag cctacttggg agcgggtacg ggcggcgatt
     6961 agcaactacc tttatagcct gtggcaacag gggggattag ctggcagcaa agaagaagac
     7021 gcttattttg tgcaaattgg taaaggtata acgatgacac aggagcagat tgatgcaggg
     7081 caaatgattg ttaaagtcgg tttggctgct gtacggcctg cggaatttat cattctccag
     7141 tttacgcaag atgtagaaca gcgttaatca tatgattatg aggagttatc atgtctgcta
     7201 ttctgaaagc gcctggcgtt tatattgaag aagacgcttc cctagcgttg tctgtcagta
     7261 acagcgcgac tgccgtgcct gtttttatcg gaaaatttac tccgacagtg gttgattcaa
     7321 tccaagtctg tacccgtatc agcaactggc ttgaattcac ttcctctttt tccctagctc
     7381 caacagttga gattgttgtc caatctaaca ctgaatctga atctgaatct gaaacttacc
     7441 actatattga gacaatcaat ttatctccag ctgtggaagc attgcgactc tattttcaaa
     7501 atggcggagg agcttgctat atctacccat taaatgatgc tgaagatgaa ttggttctgg
     7561 cggccatacc agaagtcatt gaacagaaag gtgatattac tctgttggtt tgcccggaac
     7621 tcgatctgga ttacaaaact aagatctatg gcgcagtgag ctcactgttg aatgataaca
     7681 aagtgggcta tttcctgatt gcggatagca atgatggaga atctgtgtca ggagtatgga
     7741 atagtgctaa ggccgccgcc tattatcccc agttggaaac taacctaaaa ttttccacgt
     7801 tgcctgggga taaggacatt cgtatcagcg gttatcagga tgatgatgaa acacataaac
     7861 cgaaaaactt ggatgagctc aggacaatca acgaggcgtt ggcacaggat attgatgcaa
     7921 gattgctcga ggagaaacaa cgtgctgtca tcattccgcc aagtgctgcc attgcgggca
     7981 tttattgcca aacggataat cgtcgcggtg tttggaaagc gccagccaac gttgcgctca
     8041 cagggatcgg gagtttgctt gataaggtag acgatgaacg gcagggagag atgaatgaca
     8101 agggaatcaa tgtcatccgt tcatttaccg accgtggttt tatggtctgg ggagcccgta
     8161 cttgtgtgga cgctgccaac atcagctggc gttatattcc tgttcgtcgc ctgttcaatt
     8221 ccgttgaacg agatatccgc caggcgctgc gcgctgtgtt gtttgaaact aatagtcagc
     8281 ctacctgggt acgtgctaag gctgccgttg atcaatatct ttataccctt tggcagaaaa
     8341 atgcattgat gggtgctcgc ccggaagaag cttattttgt gcaaattggt caggatatca
     8401 ccatgtccga ggctgatatt aaacagggta agatgatcat gactgttggt ttggcagcag
     8461 tgcggccagc tgagttcatc attctgcaat ttacgcagga tgttgttcag taatctccat
     8521 gactaaacgc caggcactgt attgacagtg cctactctaa ccatcttgga ggaggtgatg
     8581 atgatggaga gactccaacc gggtgtgact ttaacagaaa gtataatcac gatgggtcag
     8641 caagagatac ccagtgctgt gccggtgttt attggttaca ccgttcgtta tccggaacaa
     8701 tcggaagcat cagtccgtat cgacagtttg gccgagtata ccagcctgtt tggtgacgac
     8761 catgtgatga tgtttgctgt caggcactat tttgataatg gcgggcaaca ggcatttgtt
     8821 ttacccctga aggacaatat gccatcagtg gagatgacca cagctgaagc ggaaaatctg
     8881 atagccgcat tgcgctctgc tacggttagc gaagccattg gtgggcatag tcagattaca
     8941 ctgattttgg taccggatat ggctcggctt aatgacagtg atattgatga ctcctcaacc
     9001 caggtaagcc tgtggtccca aggctgggag gcgctgctgc aattgagtca ggttaggccc
     9061 aacctctttg tgctgttaga tgcgccggat aatgttgaac aggcgcagaa gtgtatgaca
     9121 acgctatcgt cagattatcg tcaatggggg gcagcatatt ggcctcgtct ggaaactacc
     9181 tatcagaaag aaatatctgg caaggacaat gaatctcagg gaattttcca ggggactgtt
     9241 ctgtcaccca cagccgcggt cgcagcggta attcaacgca cggataacga cgcgggtgtt
     9301 tggaaagcac cggccaatat tgccttatcc caggttattc gacctgttaa atcttatctt
     9361 cagggaagtg tactgtttaa cagcagcggc acttcgctca atgtgatccg cagtttccca
     9421 ggtaagggca tacgggtatg gggatgccgc actctggaaa acacggataa tacgcagtgg
     9481 cgctatctgc aaacacgtcg gctggtttcc tatgtaacag cgcatttgac ccaattggct
     9541 cgcatgtatg tcttcgagcc aaataatgaa cttacctgga tgaagttaaa aggacaaagt
     9601 tacaactggt tacggcaatt atggttgcag ggtggcttgt atggttcaca ggaggatgag
     9661 gcatttaaca ttctgttagg cgtaaacgag acgatgactg aggatgatgt tcgtgcagga
     9721 aaaatgatca tgaaagttga gttggctgtg ttgtttcctg ccgaatttat tgagatcagt
     9781 ttggtgttta atacccaaac agaggcgctg tcttaagaag gaaaaagtac gatgaacgat
     9841 tattacacac ccgtggtatc ccatcgtttt atggcgagtt ttatttttaa ccgcattccc
     9901 gatccgctgg atattcgttt tcagcgtatc tctggcctta gtcgggaact acaggtgact
     9961 cagtacagtg agggaggaga aaatgcccgt aataactatt tagctgagaa aatccaacac
    10021 ggtacgttga ctttggaacg gggcgtgatg acagtctcgc cattgacctg gatgtttgat
    10081 cgggtattga gtggtgaaaa aatcgcttat gccgatgtgg tggtgatgct actgaatgaa
    10141 aattcactgc cattgtccag ttggacgttg agcaatgcgc tgccggtacg ctggcaaacc
    10201 agcgactttg acgctaacag caatgccata ttggtgaata cccttgaatt gcgttaccag
    10261 gatatgcgct ggcttggagt caaaatatga cagtagaaat cagagagtta cttatccagg
    10321 caaaggtagt gccatcaaca cgaccgactg aatcagaacg gcaaaaccat tctttgatac
    10381 aggaaagtct ggatgaggcg acttgggtgg aaacgataaa acgcgaagtg ttggccgcat
    10441 tacgcgatga ggaagggtgg cgtccatgag tctgattgaa cgtggtttag ctaagctgac
    10501 aattaatgct tataaggata gggaagggaa gatacgggca ggaacgttgc aggccatgta
    10561 taaccctgac tccttgcaac tggattacca aacggattat cagcaatccc aagcgattaa
    10621 tagcgaaaag caaagtagca tttatgtaca ggccaagccc gcagggttat cacttgaatt
    10681 aatttttgat gccacgatgc cgggtaacaa aacccccatt gaagagcagc tcatgcagct
    10741 caagcaactg tgcagtgtgg atgcaaccag taacgagacg cgattcctgc aagttaaatg
    10801 gggcaaaatg cgttgggaaa gtcggggtta ctttgctggc agggccaaga gtttgtctgt
    10861 gaattacact ttgtttgatc gtgatgcgac tcccttgagg gtacgggtaa tattggcatt
    10921 agtggctgat gaaagtctgg tgttgcagga gactgaacaa aatctgcaat ctccggcaaa
    10981 aatcgcatta cgcatacagg atggggtatc tctggctctg atggcagcca gtacggcatc
    11041 aacattgtca ggcggtgtgg attatctgac gctggcctgg caaaacggtc tggataatct
    11101 caatgggttc gttccgggtg aaatattgca ggccaccagg ggagacgaat catgagccac
    11161 caactgaaaa ttattgcaga tggtaaggca ctgtcacttt tggccgcggt agatgtggac
    11221 acctgttatc gggttaacag tataccttct gcgacattga aactgagcgt accggatagg
    11281 ccactctctt ctttcagtca gacggatgtt cagacagaac tggcccactg tcaggtaggg
    11341 aaaaccctgc gtctggaatt gattgatggt agcaaaaaat gggtgctgtt taatggtctt
    11401 attacccgta aggctctgag aattaagaat aagcaattat tgctcactct ggttgtcaag
    11461 catcggttgc aactgatggt ggatacccag cattcacagc tgtttaaaga caaaagcgaa
    11521 aaagcgatct taagcacgct attgaatcag accggaatca atgctcgctt cggaaagata
    11581 gcggcgttag atcaaaagca tgaacagatg gtgcaatttc gttgttcaga ctggcatttt
    11641 ctgttgtgcc gactgtcggc aaccggtgca tggttgttac ctgccataga agacgttcag
    11701 tttgttcaac ctgatgctct gaaatcaaac tcagcctata ccttgaagag caggggggat
    11761 gagaacaaag acatcgttgt caaggatgct tactggcagt ttgacaatca aatcaacccc
    11821 gctttgctgg aagtcagtgg ctgggatatc agtaagcagc aggtacaatc aggcggtcgc
    11881 tacggaaaaa tcgcgttggg taaggcggca ctctctcctg atggattggc atcccttaat
    11941 aaaacgggtt gggacatttg ttatagcagt ccgttaacaa cccaggaaag cggttatctg
    12001 gcacagggat tattgcttaa ccagcgcatt tctggggtga caggagaatt tttgctcaaa
    12061 ggagatgggc gttaccagtt gggagacaac attcagctga ctggatttgg ttcacagtta
    12121 gatggtacgg caagcattac tgaggttcgc caccgtctta atcggcgaat tgattgggaa
    12181 accacggtga gcattggttt acaacatgaa tatttgccga tattacctga tgctcccgaa
    12241 ctacatattg cgacagtagc gaaatatcag caggacagtg cggtgttaaa ccgtatcccc
    12301 attattctgc cggtactgaa tcgtcccaat gaatttttgt gggccagatt ggggaaacct
    12361 tatgctagcc atgaaagcgg tttctgtttt tacccagagc caggtgacga agttattatt
    12421 ggtttttttg aaaatgatcc gcgttatcca gttattttag gtgctatgca taatccgaaa
    12481 aataaggccc cttttgaacc aacccaagat aatagggaaa aagtattgat cgttaaaaaa
    12541 ggtgaagcgc aacaacaatt agtcattgat ggcaaagaga aaatgatccg aattaatgcg
    12601 ggtgaaaatc aaataatgct tcagcaagat aaagacattt ctctgtcaac gaaaaaagaa
    12661 ttaacactga aagcgcagac aatgaatgcc acgatggata aatcattggc aatgtccggg
    12721 aaaaacagtg ttgaaatcaa aggcgcaaaa attaatctta cccaatgaaa ggtgacgatg
    12781 aatggaaaat caaatactga cacaactcta tggtcgtggt tgggcttttc ctccggtctt
    12841 ttcccttgaa aagggggtag agatggctga aggggcggaa gatgtgagac aaagtttgca
    12901 gattctgttt agtactgagc cgggggaacg tcttatgcgt gaaaattatg gctgcggatt
    12961 aaatgatttt atgtttgaaa atatccgcaa tgaacttatt gctgaaattg aatcccatat
    13021 ccatgacaac gtattacgat atgaaccccg ggctgatatg actgatattc aggttcgtca
    13081 atcccctggc atggggaata ctttgcaagt gcaggtcatg tatcgcctga gagggagtga
    13141 tatcaatcaa caaatccagg gagtacttgc actgagtgaa ggccgggtga cggaggtagt
    13201 atgattcatc aggaaaagga cgttttatca ctcaacaaaa ttttgctacc gtaaattaga
    13261 gtattgactg aattaaatag aattaacgaa ggtgtaaata attatttatt tgctgacgaa
    13321 tcgctgtgac aaataaacac aggtgatgtt atggaattaa atgagttaac taacaaattg
    13381 tcaaatttgg tgccaatgac cgattttaaa ttagataatc gagccagttt gcaattgctt
    13441 aaatatattg aagcgtatac gaagataata ccctttaatt ctggcgataa atattggaat
    13501 gactttttct ttatgtcagg aaatacgcca gagaaacttg caaaattata tcagaaagaa
    13561 atagaaccca atggggagtt attacctcag caggcttttt tgttggcggt tttgcgttta
    13621 ttggaaacac caatatcctt attaaatgta ttacctgctg ctcatcgtga gctctattat
    13681 cgggagcttt taggcttgtc ttcccatgcg gcacagcctg atcaggttgc tttatctatg
    13741 gaactgaatt cgacagtgat ggaacagctg ctccctgaag gaaccctgtt tgaggctggt
    13801 caggatgaac aaggcaatgc attgcaatat gccctggatg ccagtttgct ggctaatcgt
    13861 ggatatatca gtgacttgcg ctggttacgg aatgacgggg aaaagcaatg ggttacttct
    13921 gctccatggg atttacaggc acaggtgtca ctgccgtctg atgggatacg attatttggt
    13981 aagacaaata gtgatcagca ggtatttggt ggggtgttga taacgtcatc acttctggcg
    14041 atggaagcgg ggataaggaa gatcattgtt acttttgagc aggagatgaa cacccaagaa
    14101 ctggtggcac aggtcagcag tggaaatcaa tggctaacat tgacgtctga ggtaaataag
    14161 aaagaggtca cactgacact gtcagacaaa gaaccggcaa tcagtgcgcc agaggatctg
    14221 gataatctct ttttcacgca accggtactc aggctacagg gaaaggatag tcaggcactg
    14281 ccggaggtga cgggtatcag cgtttcggaa aaggatgata ctaaggatac ctcttttgag
    14341 atgtatcact taacaccatt tggttatagc agtgatatag agccattgga ggaaaatcca
    14401 gcgttatatt taggctttac tgatgtaaag ccagggcaaa cactggcgct gtattggaaa
    14461 ttaaaatccc cgcagcaacc aaccgtttcc tggtattacc tggatcaaca taatcaatgg
    14521 gctgaattgg attcatgggt cagtgatgga acccagaatc tgtatcagga tggtacttgg
    14581 cacgttgagt tgcctgtgga tgcatccaat caggcagagc agatgccagt tggacgctat
    14641 tggttgcggg cagtggtgga ggtacccgct catgaggggg cgttggggaa ggctccttgg
    14701 ctatatggtc taatctataa cgccatgacg gcaaccttgg ttaatgtaga tagcatcagt
    14761 gacagccatt tcttaacccc tttgcctgcc agcagcatac agcggcccgt tgaacccatc
    14821 attgtgttgg catcggtcaa ccagccttgg gcatcatggg gtggacgtat acctgaatcc
    14881 tacagtgcct tttttgaacg gatagctcaa aacctgtctc atcgaaaccg gtccttaacc
    14941 tggggaaata tggtgacatt actcaaagag cgttatgtca gcatctttga tgttaagtat
    15001 ccaggtaatg atgaactcac cagagtgcca gcattggagc agcagcaact aacagtgatt
    15061 ccagcaaacc ggtacaacga tagcgatgat tctctgcgtc cggtactgaa tcctgctcgt
    15121 ctgcaagaga tggctgattg gttgcagcag aaagactctc cctgggcctc tattgaggtc
    15181 aggaatccag aatacttgga tgtgaaaatc cattacgagg tgatttttaa acctgatgtg
    15241 aacgaagatt ttggctatcg ccagctacag cagcaactgt gtgaggtgta tatgccttgg
    15301 agcatagatg agcagcggcc cgttgtattg aataacagca ttaattattt ccagttgtta
    15361 gccactattc aacagcaacc gctggttgag cgagtcactc gtctgacact acatcgggct
    15421 gattcttctg atgagagtga tggtacagca tctgtggaag ccaaagataa tgaagtgctt
    15481 attttagtct gggaagagga cgataatctg caataccgag gaaatgacta tgagtaatca
    15541 ggatgcactg tttcatagcg ttaaagacga tattcacttt gataccttgc tggaacaagc
    15601 tcatcaggtg attgaaaaac aggctgaaaa actgtggagt gatacggcag agcatgatcc
    15661 gggtatcaca tttttgcagg gaatcagtta cggtgtgtca gatttggctt accgacatac
    15721 attacccctg aaagatttac tgactccggc gccggatgag cagcagcaag agggaatttt
    15781 tcctgccgaa tttggcccgc ataatacact gacttgtggg ccggtgacag cggatgatta
    15841 tcgcaaggca ttgttagatc tacacagcag cgacagcctg gatggtactc agcaggatga
    15901 gggggatttt ctgttccgga gtgtgcaact ggtgcgtgaa ccggaaaaac agcgttatac
    15961 ctattggtat gatgcaacca agagggaata tagctttgtc aacagtgaag gggctaaaga
    16021 gtttaccttg cgggggaatt actggttgta tctggaacca acccgttgga ctcagggtaa
    16081 tattgccgct gctaccagac aactgacaga atttttgact aaaaatcgca atattggtga
    16141 atctgtcagc aacattatct ggctacaacc ggttgatctg ccactgttgc tggatgttga
    16201 actggatgat gatgtaggtg cacaggatgt ccccggtatt tttgcggcgg tgtatagcac
    16261 cgcagagcag tatctgatgc ctggagcaca gcgttaccgt acggaagtac tgcaaaatgc
    16321 tgggatgagc aatgatcaaa tcttcgaagg tccattattg gaacatggct ggataccaga
    16381 gctgccggca gcccgtgatt atactcaaag gctcactctc aatcttagcc ggttggtaaa
    16441 tagtctgctt gagattgagg gcattaaaca tgtgaatcgt cttcgtctgg atgatagctt
    16501 cgataaaact gctattgaac ccgttaaggg ggatacctgg tcgtggtcga tcaaagaggg
    16561 ctattatcca cgtctttggg gagaagaccc acttaaccaa ttggcgcaac aaaatggccc
    16621 gcttagggtg atagccaaag gagggattag cgtcagtgtg agtaaagagc aaatccaggc
    16681 cagtttaccc agtcaatcac tgattcaaaa tgagccggta atattggctt acggccagca
    16741 ccgtgacgtt ggcagctatt atcccgtcag tgatactttg ccgccttgct atggactaca
    16801 acattctttg tctgaaagtg aacacttatt gccacttcat caatttatgt tgccatttga
    16861 acaattattg gcctgtggtt gtcaacagat agccatgctc ccgcggttac tggcttttca
    16921 gcgcgaaggt tatgaggttt ggggtgatca gtggcccttt aagtcaggct cagtgaatga
    16981 tgacgcccat caagattatg cccctgcatt aaaggatttg ttaggacaga ttgcgctgga
    17041 tagtgatcat gaattggata ttattaatta cttgctgggt tactttggca cacagcgggc
    17101 accgcgtacc tttacgacac aactcgatga ttttcgtgcg gtccaacagg gttatctggc
    17161 ccagcaaccg acattgactt accaccgctc caatattcgt atcgatcagg tatcgtcgct
    17221 acaaaaacgt attgctgctc gcatggggct gggcggtgag ttgtttaaac ctcaaccgga
    17281 tctgagccaa ctgccttttt atttgattga acatcgagcg ttgctgccag tcaaacccaa
    17341 tagtcagttt gataaggaac agaaaccagc ctcggtgaca gaggaggggg gcagccaaac
    17401 aggtcaacat tatgtggtca ttgaacagaa gggcattgat ggcaagctga cacaggggca
    17461 agtgatcaat ttaattctgt atgaaggaga gcagggagaa acccaattta cgatacgcgg
    17521 tcagatggta ttcaaaaccg agggggataa gttttggttg gatgtgaata atagtgcgca
    17581 actggaatat aatctggcgc gggtaatgac agcagccaag gcgagtaaac tcttttggca
    17641 aaacagcccg gtatggatgg aggatatggg ctatcgtctg gcctatgcta gtgaccaatc
    17701 ctcattgcct gtgaatcaac ggcgcttgac ccgcacagtg caaactccat tcccgccgat
    17761 ggttgttgta ggtagcgaaa tcaccctgtt aaagcaggtg gggatagtca atttaaaaaa
    17821 agcggagtca gaaaaacttt atgcaaaagt tgttagcttt gatcgcattg aagggacctt
    17881 gattattgag cgtttgggta attccactct ggcttttcct acctcggaag aggcgtggcg
    17941 gtatagttgg tatttttcgg gggagaaata tgaaaggact gaccgctttt catttgtgat
    18001 tagcgtagta gtgaacagtg acttaattaa attgcccggt gttgatccct ataaattgga
    18061 agaatgggtg aaagaaacga ttcttaccga atttccagct catatttcta tgattatcca
    18121 ttggatggat cgggaagcct ttttaaattt cgccaatacc tatcagcgtt ggcaaaataa
    18181 tggtacgcca ctgggggatg cggcttattc cattctagaa agtttgacac ttggtaaatt
    18241 gccatctgcc ttaaaaggtg ttggcacaat gcgtattgcc acatctagtc aaagagaaga
    18301 agtggtgggt agtaatggtg atcaatggaa tacagatgga ataacccaga atgaattatt
    18361 ctatgttcct aaagagagct aggaaaaata aatatctgcc actaatgatg ttgaattaaa
    18421 tatgttttct ggagttaatc atgaacgaaa ctcgttataa tgcaactgta caagaacaac
    18481 aaacattatc taatccaaaa gctgttggac ctgacatcga taaattaaag gataaattta
    18541 aagagggcag tattcccctg caaaccgatt tcaatgagtt aattgatatt gccgatattg
    18601 gacgtaaagc ctgtggtcaa gcgccacaac aaaatggccc aggagaagga ttgaaattgg
    18661 ctgatgacgg tacgcttaat ttaaaaatag gcactttttc caataaagac ttttctccat
    18721 taatattaaa agatgatgtt ttatctgtag atcttggtag tggtctgact aatgaaacca
    18781 atggaatctg tgtcggtcag ggcgatggta ttacagttaa cactagcaat gtagctgtaa
    18841 aacaaggtaa cggaattagc gttactagta gtggtggtgt tgccgttaaa gttagtgcta
    18901 ataagggact tagcgttgat agtagtggtg ttgcagttaa agttaatact gataagggaa
    18961 ttagcgttga tggtaatggt gttgcagtta aagttaatac tagtaaagga attagcgttg
    19021 ataatacagg tgttgcagtt atagctaatg ctagtaaggg aattagcgtt gatggtagtg
    19081 gtgttgcagt tatagctaat actagtaaag gaattagcgt tgatggtagt ggtgttgcag
    19141 ttatagctaa tactagtaaa ggaattagcg ttgataatac aggtgttgca gttatagcta
    19201 atgctagtaa gggaattagc gttgatggta gtggtgttgc agttatagct aatactagta
    19261 aaggaattag cgttgatggt agtggtgttg cagttatagc taatactagt aaaggaatta
    19321 gcgttgatag tagtggtgtt gcagttaaag ttaaagctaa tggcggaatt aaagtagatg
    19381 ctaatggtgt tgcaattgat cctaataatg tactccccaa gggagtgatt gtaatgttct
    19441 ctggcagtac tgcaccaact ggttgggcgt tatgtgatgg caataatggt acaccaaatt
    19501 taatcgatcg atttatttta ggtgggaaag ggactgatat taatggagtg agtactaata
    19561 cagcttcagg tactaaaaat agtaagttat tcgatttcag ttctgatgaa gctacattaa
    19621 ctattgatgg taaaacactg gggagaggtg gctctggtgg tcgtggtgtt ccgcatattg
    19681 ttatggttga tgcctataaa cgctataaag gcggttctgg cggccacgat catgatatta
    19741 agataacggg cacaggaaaa cattctcaca aaaacaaagt aacagtccct tattatattc
    19801 tggctttcat cataaagctt taatatatat gaaaaattga aaatataaat tatccattaa
    19861 taataaagag gatattagca tgacttcgga gccaaatctg ttaaaccgga ttacaattac
    19921 tattgaagct aataatcaac aagtagctag aaaagtattg catggctcct tgcttaatca
    19981 agctaatata aataaattat ttaattcata ctttaatgaa tatgaaatta ataggggtgt
    20041 ttatttagaa acattaatcc tgaatcttgg tacgataaat ttccatgatt ttaattcatt
    20101 gtttcctact ctcctaaaag ctgcattgaa taaagaattc agtcaatatc agataaacaa
    20161 ccatagggaa gaaatgctat ttaatgagac aatatcaaat caagctactg ataagtctta
    20221 catatttggc gataacaaat taattgatgc agagaatttc attcactttt tatatcaaaa
    20281 gcattccaca ttaaatctag tagaagcaat gggaaataat ggtattgaaa aattaacaaa
    20341 tcagttaaca caaatagaaa ataaatttgc gttattattg gcaaaaagtt gtttgtctga
    20401 ggaaggctta aaacgactct tggctatcaa acaacccgat ttattaatcg ctatcaatcg
    20461 cagattatct gaaagaataa atagaccaca atatcaggag aagcttgttt cctgcggaca
    20521 actgatattt agtgctctgg gatatataca acagtacaat atacaggaaa ttcctaaacc
    20581 ggatgaaaaa gttattgcac gcataacaac tgaacttaat aataatggtt tgcttaatac
    20641 aatacctatt attacactat ttcgtcagag tgggattaac gattcatcac taaatgattg
    20701 gctaaagaaa atctggcagg tgagatcaat ttcacagtta tgcagaaagt atctttctgc
    20761 taaggaatac caatatctgt cagaacattt tgtttcaaag agcgtcgata aaaatagata
    20821 tgatgaagag cccgtaaatc agagcatatt atcaaggttg aataataatt ccattaaaga
    20881 aggaaataat cacagtcaac tctgtactct cagtagacta tattctgaac ccgttgtatt
    20941 acctgaacaa accattctac gtcaggttag taatacagta gatcagagca tattatcaag
    21001 gttgaataat gcctccatta aagaaggaaa taaccaaagt caacttcgca ctctcagtag
    21061 actatattct gagcccgttg cattacctga acaaaccatt ccacgtcagg ttagtaatac
    21121 aggtatatta attctatggc caatgctacc tacactattt aaccagcttg gtctacttga
    21181 gaaaaagaaa tttatccatc gtcaggccca gtttaatgcc gttgattttc ttgattacct
    21241 gatttgggga accgaagatg tgaaagtgga acgaaaggtt ttgaataatg ttctatgtgg
    21301 gttaatggct gatgaaatta ctgaaccaat gcctattgaa ccagaaaaac aatggataat
    21361 aattcaatgg ctggacgcta ttatctccca actttctggc tggaaaaagt taagtcgtaa
    21421 tgacgtccgt caattatttc tacaacgacc aggagaatta ctgatcaatg aacaggaaat
    21481 taaaatcaca atacagcaac aaccatttga tgctctgtta actgattggc cgtggccaat
    21541 gaatatggct tgttttagct ggttgagtca accattaacc attacgtggt tataaccatt
    21601 gaccacaatg acttagtctg agtaaaaaat atgaatatat cgcctgtttt ttatgattca
    21661 ttgaatcagg ataacgaccg tgatctatcg tttttattta gcgaactgga acgaatagat
    21721 ctcgctcttc aacaccattt ttattgtgta gaaagtcagc gaagtgagct cctggatgag
    21781 tttctgctca ctgaggcgga agtggtgacc aggctggata agccacttgg taaacctcat
    21841 tggataaatg atgattatct ggcgatatcg caaaagggca atgtaagcct aatggcagcg
    21901 tccagattaa tggatctgat cgaacgcttt gaactgactg attttgagcg cgatgtttta
    21961 ctattaggct tattgcccca ttttgatagc cgctattatc gactgttttc gctgattcaa
    22021 gggggacaac agggtcgatt accttctttt gcgctggcat tggaactgtt ttgccactcg
    22081 gcgctggaga aacaggtaca gcaagcgagt tttctgcacc gggcaccttt gatgggttgc
    22141 cagctattat ccatcgatac tagtcaaaaa acgctggcct ggctccagac tccctttatt
    22201 actgacagcg gggtatatca ctttttactg gggcatcact acattatgcc ggctttagaa
    22261 cattgtgctg agtggttaac accgacaggg attggctgtt atcctgaagg attaaaacaa
    22321 gtactgggta acgtattgtt atctgacaac gataatatta gaccgattgt cttattacgg
    22381 ggaatggccg gcagtgccag agcttatacc attactaata tgatggcttc agaagggaag
    22441 caaacactgc tggtagatat atccaaactt gctgatagcg atgaaaaaaa cattattctt
    22501 cagataaagc atattttgcg ggaaacccgc atgcatggag catgtttatt attacggaat
    22561 ttttgcttgt tagtggaaca gaataaacaa ctattggact ccctgtcaga gttattgaat
    22621 caacctgaat taagaattgt ttgcctgatt gagccttatt ccccattggt atggctgaaa
    22681 aagataccgg tattactgat tgagatgcca cttttaacgc ctgcggaaaa agccagattg
    22741 ttaattgcca gcttaccgga taattgttcc gaggatattg atacgataac tttaagccag
    22801 cgttacactt ttaacccaga aaccctgcca ttgattttgc aagaggccca gctttatcaa
    22861 cagcagcgag atccgctgga tatcttgcag caatgcgata tacgccaggc attaaatttg
    22921 cgtgctcaac aaaatttcgg tcaattggca cagcggatta ttcctaagcg ctcattaaag
    22981 gatttattgg tatccgatga gattgctcag cagttacggg aaatactcat agcaattaag
    23041 tatcgggaac aggttctggc gggagggttt aaagataaaa ttgcctatgg cactggtatc
    23101 agcgccctgt tttatggtga ttcaggcact ggaaaaacca tggcagcaga agtgattgct
    23161 gaccacattg gcgttgactt aataaaagtg gatttatcta cagtagtgaa taaatacatc
    23221 ggtgaaacag aaaaaaactt atcccgtatt ttcgatttgg cggaacagga tgcaggggta
    23281 ttattctttg atgaagctga cgcactgttt ggtaaacgca gtgaaactaa agattcccag
    23341 gacagacatg ccaatattga agtttcttac ttattacagc gcctggagaa ttacccgggt
    23401 ctggtcattt tatccaccaa taatcgtggt catttagaca gtgcttttaa tcgtcgtttt
    23461 actttcatta cccgttttac ttacccggat gaaaaaatcc gtaaaaaaat gtggcaggaa
    23521 atttggccta gaaatataaa aatatcggaa gatatcgatt ttaacgaatt agctcaacga
    23581 acaagcgtga ctggcgcgaa tatccgcaat attgctttat tgtcttcatt ctttgcttca
    23641 gagcagggga atgatgaagt cagtaatgaa aatattgaaa ttgcattgaa gcgtgaatta
    23701 gctaaagtcg gacgattaac attttaaaag ttatcacaat gaaagtattg aaatattaaa
    23761 taaatttatt accaaaaagt tatcacgata taatttaaga gaggtttttt atgttaaaca
    23821 cgcaaactat tattgatgtc aataaggcaa tggatgccat gctgcgcgca tatctgaatc
    23881 aagatattgc cattcgtttt gatctacctg aattggatac tatgcaatct gatgcgatgg
    23941 taagtatctt tctttatgac attcatgaag atttacagct tcgctcggca gaatcaagag
    24001 ggtttgatgt ttatgccggg aggttattgc ctggttgggt aaatattaaa tgtaactatc
    24061 tgattaccta ttgggaagct tctaagccag cgactgatgc cagcagtccg gatagccaac
    24121 ctgataacca ggcaatacaa gtgatgtcac aagtattaaa tgccttgatt aataatcgtc
    24181 aattggcagg tattcctggt gcttatactc aggttgtacc gcctaaagag agtttaaata
    24241 gcctggggaa tttctggcaa tcactgggta atcgcccacg gctttctctc aattattcag
    24301 tgacagtacc tgttagccta aacgatggtc aggatagcgc gactccggtt accgcggttt
    24361 cttctacagt ggaacaaacg gcatcgctca gtcaagaagt ggttagtcat gctttacgcg
    24421 aattactcat tacggaatta ggaggaggag aggataaccg gttggtactg agtaaagttg
    24481 aattatccgc agtgaaagag acgatgactc aagacagtcc ggctcagatg attatattgt
    24541 tgtctgtttc aggcattaca cgacaggaat atttgaagga aattgataat atctttgatc
    24601 gttgggtaaa taatgctgaa gttattacca ctattgatga ttgtgggatt agaattgaaa
    24661 gtataacgaa agataatctt gtaggaattt aaattttttt aattaaataa aggaattaat
    24721 attgatttga tagagcatgt aatcaataac ttctatgaaa atgttgaaaa acaatacacg
    24781 gaaacactta acttacaatt aaattgttaa ag
//
