LOCUS       sequence-487090-        4714 bp    DNA     circular SYN 24-SEP-2025
DEFINITION  Bacterial expression of green glucose sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4714)
  TITLE     iGlucoSnFR2: a genetically encoded fluorescent sensor for measuring 
            intracellular or extracellular glucose in vivo in mouse brain
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pNoRBP.iGlucoSnFR2
FEATURES             Location/Qualifiers
     source          1..4714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             70..92
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     1296..1317
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1420..1441)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             2032..2049
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2115..2133)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2129..2176
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2273..2728
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2360..2379)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2570..2591
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2754..2858
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2859..2927,2928..3719)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3077..3096)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3890..4478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4379..4398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4632..4649
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
       61 agaaataatt ttgtttaact ttaagaagga gatatacata tgagatctaa gctggagatt
      121 ttctcttggt gggctgggga tgaaggccct gccctggagg cactcatcag actgtataaa
      181 cagaagtatc ctggagtaga ggtgataaac gccactgtca ccggaggtgc cggtgtaaat
      241 gctcgcgctg tccttaagac aaggatgctt ggtggtgatc ctcctgatac atttcaagtt
      301 gcagcaggga tggagttgat aggcacatgg gtggtggcta acagaatgga ggatctcagc
      361 gccttgtttc gacaagaggg ttggcttcaa gccttcccca agggccttat tgatctgatt
      421 agttataaag gtggtatatg gagtgtgccc gttaatattc acaggtcaaa cgtaatgtgg
      481 tatttgcctg caaagctaaa agagtgggga gttaaccccc cacggacttg ggatgagttt
      541 cttgccacct gtcaaacact taaacaaaag ggtctggaag ctccactggc tctcggggaa
      601 aattggacac aacaacacct ctgggagagt gtcgccttgg ctgtattggg gcctgatgat
      661 tggaataatc tgtggaatgg gaagctgaag ttcacagacc ctaaagcagt tagggcatgg
      721 gaagtctttg gccgcgtttt ggactgtgcc aacaaggatg cagcaggctt gtcttggcag
      781 caagctgtcg atcgggtagt acaggggaaa gccgctttta acgtaatggg cgactgggca
      841 gcaggatata tgactaccac tctcaagttg aagcctggta cagatttcgc atgggcacca
      901 agtcccggga cacagggcgt ctttatgatg gtttccgact ctttcggact tcccaaagga
      961 gccaaaaatc gacaaaacgc tatcaactgg cttcgattgg tcggctctaa agaaggtcaa
     1021 gatacattca acccactgaa agggtccatc gctgctcggc tcgatagcga cccaagcaag
     1081 tatccgtgta acgtctatat caccgccgac aagcagaaga acggcatcaa ggcgaacttc
     1141 aagatccgcc acaacgtgga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1201 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctggt gacccagtcc
     1261 gtgctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1321 gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg agggagcatg
     1381 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1441 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
     1501 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1561 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     1621 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cagcttcaag
     1681 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1741 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1801 gagtacaact ttaacaaccc caacgcatac ggacagagtg caatgcgaga ttggcggagt
     1861 aatcgcatcg ttggctccct ggtagcagga gcagtggccc ctgaaagctc catgtcacaa
     1921 ttcggaacag taatggaaat tttcctgcaa acacgcaacc cacaagcagc agcaaatgca
     1981 gcacaagcta ttgctgacca agttggcctg ggtcgccttg gtcagctgca gcatcaccat
     2041 caccaccatt aagaattcga agcttgatcc ggctgctaac aaagcccgaa aggaagctga
     2101 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
     2161 cttgaggggt tttttgctga aaggaggaac tatatccgga tctggcgtaa tagcgaagag
     2221 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     2281 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     2341 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     2401 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     2461 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     2521 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     2581 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     2641 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     2701 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     2761 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     2821 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     2881 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     2941 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     3001 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     3061 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     3121 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     3181 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     3241 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     3301 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     3361 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     3421 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     3481 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     3541 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     3601 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     3661 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     3721 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     3781 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     3841 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3901 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3961 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4021 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4081 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4141 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4201 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccaaac
     4261 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4321 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4381 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4441 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4501 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     4561 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     4621 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     4681 ctctccccgc gcgttggccg attcattaat gcag
//
