LOCUS       sequence-487152-        9991 bp    DNA     circular SYN 25-SEP-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-SEMA5A-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             3875..3898
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5558..5602
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             5603..5629
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(5769..5786)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5775..5886
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6004..6024)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6009..6338
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6189..6324
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6251..6270
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6386..6433
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6452..7051
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6452..6471)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6948..6968
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7181..7302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7218..7237)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7272..7291
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(7355..8146,8147..8215))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7978..7997
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8216..8307)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8349..8368
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8374..8393
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8479..9067
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8968..8987
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9221..9238
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     9336..9353
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9337..9353
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9809..9825
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg ccgaagctca gggaacaaca cagtgtcaga gaacagagca
     1021 ccccgtgatc agctacaaag agatcggccc ctggctgaga gagttcagag ccaagaacgc
     1081 cgtggacttc agccagctga ccttcgatcc tggccagaaa gagctggtcg tcggcgccag
     1141 aaactacctg tttcggctgc agctggaaga tctgagcctg atccaggccg tggaatggga
     1201 gtgtgatgag gccaccaaga aggcctgcta cagcaagggc aagagcaaag aggaatgcca
     1261 gaactacatc cgggtgctgc tcgttggcgg cgacagactg tttacctgcg gcaccaatgc
     1321 cttcacaccc gtgtgcacca acagaagcct gagcaacctg accgagatcc acgaccagat
     1381 cagcggcatg gccagatgtc cttacagccc tcagcacaac agcacagctc tgctgacagc
     1441 cggcggagaa ctgtatgccg ccacagccat ggatttccct ggcagagatc ccgccatcta
     1501 cagatctctg ggaatcctgc ctcctctgag aacagcccag tacaacagca agtggctgaa
     1561 cgagcccaac ttcgtgtcca gctacgacat cggcaacttc acctactttt tcttccggga
     1621 aaatgccgtg gaacacgact gcggcaagac cgtgtttagc agagccgcca gagtgtgcaa
     1681 gaacgatatc ggcggcagat tcctgctcga ggacacctgg acaaccttta tgaaggcccg
     1741 gctgaactgc agcagacctg gcgaggtgcc cttctactac aacgagctgc agagcacatt
     1801 ctttctgccc gagctggacc tgatctacgg catcttcacc accaacgtga actctatcgc
     1861 cgccagcgcc gtgtgcgtgt tcaatctgtc tgctatcgcc caggccttca gcggcccttt
     1921 caagtaccaa gagaacagca gaagcgcctg gctgccctat cctaatccaa atcctcactt
     1981 ccagtgcggc accgtggatc agggcctgta tgtgaacctg acagagagaa acctgcagga
     2041 cgcccagaaa ttcatcctga tgcacgaggt ggtgcagccc gtgaccacag tgcctagctt
     2101 catggaagat aacagccggt tctcccacgt ggccgtggat gtggttcagg gaagagaagc
     2161 cctggtgcac atcatctacc tggccaccga ctacggcacc atcaagaaag tgcgggtgcc
     2221 cctgaatcag accagcagct cttgtctgct ggaagagatc gagctgttcc ccgagcggag
     2281 aagagagcct atcagatccc tgcagatcct gcacagccag tccgtgctgt ttgtgggcct
     2341 gagagaacac gtggtcaaga tccctctgaa gcggtgccag ttctaccgga ccagaagcac
     2401 ctgtattggc gctcaggacc cttactgtgg ctgggacgtc gtgatgaaga agtgcaccag
     2461 cctggaagag tccctgagca tgacacagtg ggagcagagc atcagcgcct gtcctaccag
     2521 aaatctgacc gtggatggcc actttggcgt gtggtcacct tggacacctt gcacacacac
     2581 agatggctct gccgtgggca gctgtctgtg cagaacaaga agctgcgata gccctgctcc
     2641 tcagtgtggc ggatggcaat gtgaaggccc tggcatggaa atcgccaact gtagcagaaa
     2701 tggcggctgg accccatgga cctcttggag cccttgtagc acaacctgtg gcatcggctt
     2761 tcaagtgcgg cagagaagct gcagcaaccc cacacctaga cacggcggaa gagtgtgcgt
     2821 gggccagaac agagaggaac ggtactgcaa cgagcatctg ctgtgccctc cacacatgtt
     2881 ttggacagga tggggcccct gggagagatg tacagcacaa tgtggcggag gaatccaggc
     2941 cagaagaagg atctgcgaga acggccctga ttgtgccggc tgcaatgtgg aataccagag
     3001 ctgcaacaca aacccctgtc ctgagctgaa gaaaacaaca ccctggactc cctggacacc
     3061 cgtgaacatc tctgacaatg gcggccacta cgagcagcgg ttcagataca cctgtaaagc
     3121 ccggctggcc gatcctaacc tgcttgaagt gggcagacag cggatcgaga tgagatactg
     3181 tagctccgat ggcaccagcg gctgtagcac agatggactg agcggcgatt ttctgagagc
     3241 cggcagatac agcgcccaca cagttaatgg tgcttggagc gcctggacaa gctggtccca
     3301 atgtagcagg gattgcagca gaggcatccg gaaccggaag agagtgtgta acaaccccga
     3361 gcctaagtac ggcggcatgc cttgtcttgg ccctagcctg gaatatcaag agtgcaatat
     3421 cctgccttgt cctgtggacg gcgtctggtc ttgttggagt ccttggacca agtgctctgc
     3481 cacatgcggc ggaggccact acatgagaac cagatcctgt agcaaccccg ctcctgccta
     3541 cggcggagat atttgtctgg gactgcatac cgaagaggcc ctgtgcaata cccagccatg
     3601 tccagaatct tggagcgagt ggtccgattg gagcgaatgt gaagccagcg gcgtgcaagt
     3661 tcgggccaga cagtgtatcc tgctgttccc tatgggctcc cagtgcagcg gcaataccac
     3721 cgaaagcaga ccctgcgtgt tcgacagcaa cttcatccct gaggtgtccg tggccagatc
     3781 cagcagcgtg gaagagaaaa gatgcggcga gttcaacatg ggcgcgccgg aagatcaagt
     3841 tgatccaaga ttgattgatg gcaagggcgg atctctggag gtgctgttcc agggaccagg
     3901 cggaagcggc aatcccggcg gcgatctggc cccccagatg ctgcgcgagc tgcaggagac
     3961 caatgccgcc ctgcaggacg tgcgcgagct gctgcgccac cgcgtgaagg agatcacctt
     4021 cctgaagaac accgtgatgg agtgcgacgc ctgcggtatg cagccctcta gaggctccgg
     4081 ctccatcatc ccagttgagg aggagaaccc ggacttctgg aaccgcgagg cagccgaggc
     4141 cctgggtgcc gccaagaagc tgcagcctgc acagacagcc gccaagaacc tcatcatctt
     4201 cctgggcgat gggatggggg tgtctacggt gacagctgcc aggatcctaa aagggcagaa
     4261 gaaggacaaa ctggggcctg agatacccct ggccatggac cgcttcccat atgtggctct
     4321 gtccaagaca tacaatgtag acaaacatgt gccagacagt ggagccacag ccacggccta
     4381 cctgtgcggg gtcaagggca acttccagac cattggcttg agtgcagccg cccgctttaa
     4441 ccagtgcaac acgacacgcg gcaacgaggt catctccgtg atgaatcggg ccaagaaagc
     4501 agggaagtca gtgggagtgg taaccaccac acgagtgcag cacgcctcgc cagccggcac
     4561 ctacgcccac acggtgaacc gcaactggta ctcggacgcc gacgtgcctg cctcggcccg
     4621 ccaggagggg tgccaggaca tcgctacgca gctcatctcc aacatggaca ttgacgtgat
     4681 cctaggtgga ggccgaaagt acatgtttcg catgggaacc ccagaccctg agtacccaga
     4741 tgactacagc caaggtggga ccaggctgga cgggaagaat ctggtgcagg aatggctggc
     4801 gaagcgccag ggtgcccggt atgtgtggaa ccgcactgag ctcatgcagg cttccctgga
     4861 cccgtctgtg acccatctca tgggtctctt tgagcctgga gacatgaaat acgagatcca
     4921 ccgagactcc acactggacc cctccctgat ggagatgaca gaggctgccc tgcgcctgct
     4981 gagcaggaac ccccgcggct tcttcctctt cgtggagggt ggtcgcatcg accatggtca
     5041 tcatgaaagc agggcttacc gggcactgac tgagacgatc atgttcgacg acgccattga
     5101 gagggcgggc cagctcacca gcgaggagga cacgctgagc ctcgtcactg ccgaccactc
     5161 ccacgtcttc tccttcggag gctaccccct gcgagggagc tccatcttcg ggctggcccc
     5221 tggcaaggcc cgggacagga aggcctacac ggtcctccta tacggaaacg gtccaggcta
     5281 tgtgctcaag gacggcgccc ggccggatgt taccgagagc gagagcggga gccccgagta
     5341 tcggcagcag tcagcagtgc ccctggacga agagacccac gcaggcgagg acgtggcggt
     5401 gttcgcgcgc ggcccgcagg cgcacctggt tcacggcgtg caggagcaga ccttcatagc
     5461 gcacgtcatg gccttcgccg cctgcctgga gccctacacc gcctgcgacc tggcgccccc
     5521 cgccggcacc accgacgccg cgcacccggg tggcggagga ctgaacgaca tcttcgaggc
     5581 tcagaaaatc gaatggcacg aacatcacca tcaccatcac catcaccatt aaggccggcc
     5641 tgtagtcact ggcacaacgc gttacccggt aagccaatcg ggtatacacg gtcgtcatac
     5701 tgcagacagg gttcttctac tttgcaagat agtcttgagt agtaaaataa atagatagag
     5761 aaaatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     5821 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     5881 tgcatcacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5941 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     6001 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     6061 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6121 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6181 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6241 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     6301 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     6361 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     6421 agtataatac gacaaggtga ggaactaaac catgaccgag tacaagccca cggtgcgcct
     6481 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     6541 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     6601 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     6661 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     6721 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     6781 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     6841 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     6901 ggccgagcgc gccggggtgc ccgccttcct ggagacatcc gcgccccgca acctcccctt
     6961 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     7021 ctggtgcatg acccgcaagc ccggtgcctg acacgtgcta cgagatttcg attccaccgc
     7081 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     7141 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     7201 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7261 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     7321 gacctctagc tagagcttgg cgtaatcatg gtcattacca atgcttaatc agtgaggcac
     7381 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7441 taactacgat acgggagggc ttaccatctg gccccagcgc tgcgatgata ccgcgagaac
     7501 cacgctcacc ggctccggat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7561 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7621 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccatcgct acaggcatcg
     7681 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7741 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7801 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7861 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7921 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7981 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8041 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8101 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8161 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatattct
     8221 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8281 ttgaatgtat ttagaaaaat aaacaaatag gggtcagtgt tacaaccaat taaccaattc
     8341 tgaacattat cgcgagccca tttatacctg aatatggctc ataacacccc ttgctcatga
     8401 ccaaaatccc ttaacgtgag ttacgcgcgc gtcgttccac tgagcgtcag accccgtaga
     8461 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8521 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8581 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8641 gtagttagcc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8701 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8761 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8821 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8881 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8941 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9001 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9061 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9121 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     9181 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     9241 agcggaaggc gagagtaggg aactgccagg catcaaacta agcagaaggc ccctgacgga
     9301 tggccttttt gcgtttctac aaactctttc tgtgttgtaa aacgacggcc agtcttaagc
     9361 tcgggccccc tgggcggttc tgataacgag taatcgttaa tccgcaaata acgtaaaaac
     9421 ccgcttcggc gggttttttt atggggggag tttagggaaa gagcatttgt cagaatattt
     9481 aagggcgcct gtcactttgc ttgatatatg agaattattt aaccttataa atgagaaaaa
     9541 agcaacgcac tttaaataag atacgttgct ttttcgattg atgaacacct ataattaaac
     9601 tattcatcta ttatttatga ttttttgtat atacaatatt tctagtttgt taaagagaat
     9661 taagaaaata aatctcgaaa ataataaagg gaaaatcagt ttttgatatc aaaattatac
     9721 atgtcaacga taatacaaaa tataatacaa actataagat gttatcagta tttattatca
     9781 tttagaataa attttgtgtc gcccttaatt gtgagcggat aacaattacg agcttcatgc
     9841 acagtggcgt tgactcaata ttggccatta gccatattat tcattggtta tatagcataa
     9901 atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat
     9961 attggctcat gtccaatatg accgccatgt t
//
