LOCUS       sequence-487156-        9557 bp    DNA     circular SYN 25-SEP-2025
DEFINITION  Zebrafish transgenesis plasmid encoding D. rerio cPla2-mKate2, 
            driven by the QUAS enhancer, and including a cmlc2:EGFP cardiac 
            transgenesis marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9557)
  TITLE     Fibroblasts promote osmotic surveillance by wound-induced unique 
            calcium patterns
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDestTol2CG2-QUAS:cPla2-mKate2
FEATURES             Location/Qualifiers
     source          1..9557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..37)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    53..187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(489..1202,1203..1205,1206..1208))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(492..1202,1203..1205,1206..1208))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(534..555)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     883..902
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1142..1163
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2664..2680)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2664..2680)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2717..2737)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2717..2735)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2756..2772)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2769..2791)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2780..2796
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2804..2810,2811..2828,2829..2834))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2849..2870
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2987..3004)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3158..3746)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3238..3257)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3917..4708,4709..4777))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4540..4559
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4778..4882)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5045..5066)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6066..6082
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6102..6122
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6313..6337
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8610..9320
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKAVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRAD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKL"
     CDS             8610..9320
                     /codon_start=1
                     /product="monomeric photoactivatable red fluorescent 
                     protein (Gunewardene et al., 2011)"
                     /label=PAmKate
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKAVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRAD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKL"
     protein_bind    complement(9327..9351)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    9353..9487
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(join(9550..9557,1..13))
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ttatacatag ttgataattc actggccgtc gttttacggt accatcgatg atgatccaga
       61 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      121 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
      181 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
      241 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga
      301 tcagatctct tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
      361 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
      421 gtatcttatc atgtctggat ctacgtaata cgactcacta tagttctaga ggctcgagag
      481 gggccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
      541 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg
      601 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      661 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
      721 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
      781 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
      841 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
      901 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
      961 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg
     1021 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
     1081 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
     1141 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
     1201 ctcaccatgg tcactgtctg ctttgctgtt ggtctgggct cctgggtcac tggcttacta
     1261 atggagtctt tatgtatgag gactcttatc aattgttctt ctataaaggt ctgcagtgtt
     1321 tctgttcgtc ccctacatgg acacccagag cctcctaaat acaggagccc tgataactgc
     1381 acaagtgctc agattccagc agggtggaaa atgagataaa gtgtgcagat ggggaggggg
     1441 acgtgaatga gagatttgag ggatgaaaag gatggatgaa cgcattgaaa atagcccctt
     1501 tcacaaaata ataccagtaa attgtcatat aattaatgac cagaccttta cctgtaaatg
     1561 atgtgatgtt catatacaga agaatgccaa caatttacag atgattttac aaattcttaa
     1621 ttttcctttt ctaaatggat ttgttcacac attatctctt caaagcaatt gaattttctg
     1681 gaaaagattg gctgtgtgtg taaaaggtgc tactgaggaa tttaatgtga catggaatga
     1741 agcaaacagc agcctaagac aggatggcag gaaaaatgtc acatgtttaa aataagagtg
     1801 aaaccaacga cctggactta tagagactgt gtgtccttag gcaaagctct tgtacaaaca
     1861 gtgcttgtga ctgtgaaatt cggggtttgc ctggattgtg ttaaatgtgt gcttggatgt
     1921 atcataaaaa agaatcggtt ctctgtattt gacattttct gtaatttatc taaagttaca
     1981 actggctatg cctgattttt atcactattg aatcaatcat tttaaagaat gttaagtctg
     2041 ctgatttgga gagctcattt acatttattt tgaatgtctc ttatttaaca caactgattt
     2101 aagctttaga tctgcgaaga tacggccacg ggtgctcttg atcctgtggc tgattttgga
     2161 ctgtgctgct cgcagctgct gatgaatcac atacttcctc cattttcttc cactgattga
     2221 ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac
     2281 atgacgtcac ttccaaagga ccaatgaaca tgtctgacca atttcatata atgtgaaaac
     2341 gattttcata ggcagaataa ataacattta aattaaactg ggcatcagcg caattcaatt
     2401 ggtttggtaa tagcaaggga aaatagaatg aagtgatctc caaaaaataa gtactttttg
     2461 actgtaaata aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag
     2521 taaaagtaaa agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa
     2581 tacttaagta cagtaatcaa gtaaaattac tcaagtactt tacacctctg gttcttgacc
     2641 ccctaccttc agcaagccca gcagatccac tagttctaga gcggccgcca ccgcggtgga
     2701 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     2761 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     2821 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     2881 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     2941 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     3001 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     3061 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     3121 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3181 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3241 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3301 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3361 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3421 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3481 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3541 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3601 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3661 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3721 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3781 ctcagtggaa cgaaaactca cgttaaggca ttttggtcat gagattatca aaaaggatct
     3841 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3901 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3961 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4021 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4081 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4141 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4201 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     4261 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     4321 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4381 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4441 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4501 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     4561 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4621 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4681 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4741 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4801 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4861 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt
     4921 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     4981 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     5041 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     5101 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     5161 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     5221 ttgacgggga aaggctggct aagaactcat cagcctcccc ggtccatcta cccacgtacc
     5281 aatgcaccaa ttggccacaa tgacggctac tacatggtgc cattccttcc tctttatagg
     5341 aatggagact acctcctgtc caacaatgct cttggatacg agtacgccta cctgttggac
     5401 ccaggtcatt gcacaacacc agaaatgccc tctgatctgc aaaagacgtg aatatctgtt
     5461 cagacaccca tatccactct gttccacaca ggtcagaggt ttgtccagga gttcttgaca
     5521 gaggtgtaaa aagtactcaa aaattttact caagtgaaag tacaagtact tagggaaaat
     5581 tttactcaat taaaagtaaa agtatctggc tagaatctta cttgagtaaa agtaaaaaag
     5641 tactccatta aaattgtact tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac
     5701 tagagattct tgtttaagct tttaatctca aaaaacatta aatgaaatgc atacaaggtt
     5761 ttatcctgct ttagaactgt ttgtatttaa ttatcaaact ataagacaga caatctaatg
     5821 ccagtacacg ctactcaaag ttgtaaaacc tcagatttaa cttcagtaga agctgattct
     5881 caaaattgtt agtgtcaagc ctagctcttt tggggctgaa aagcaatcct gcagtgctga
     5941 aaagcctctc acaggcagcc gatgcgggaa gaggtgtatt agtcttgata gagaggctgc
     6001 aaatagcagg aaacgtgagc agagactccc tggtgtctga aacacaggcc agatgggccc
     6061 tcgagcagga aacagctatg accatgatta cgccaagcta tcaactttgt atagaaaagt
     6121 tggggtaatc gcttatcctc ggataaacaa ttatcctcac gggtaatcgc ttatccgctc
     6181 gggtaatcgc ttatcctcgg gtaatcgctt atccttgcaa gggtcgactc tagagggtat
     6241 ataatggatc ccatcgcgtc tcagcctcac tttgagctcc tccacacgaa ttccctcgac
     6301 ctcgaagacg cgtcaagttt gtacaaaaaa gcaggctgga cggatctatc gatgccacaa
     6361 tgtccaacat tatagttgag catcagtatt ctcatagact gaaattgact gtggtgagag
     6421 ctgaaaatgt aactaaagga gcatttgggg atttattgga caccccggac ccttatgttg
     6481 agctgtcagt gccaaccaca ccagagtcaa gaaaacgaac ccgccacatt aacaatgaca
     6541 ttaaccccaa gtggaacgag acctttgagt tcatattgga tcccaatcaa tccaatgttt
     6601 tggaggtgac actgatggat gccaattatg tcatggatga aactcttggc acagcaaaat
     6661 attccctctc aaaactcaaa gtggcacaaa tggagcatgt gaccctctct atcggcaaga
     6721 caaccaaagt gttcctcgac ttgctcttgg aagtttgcgc atccacagac ctgcgcttca
     6781 gcatgacact gtgtgatcaa gagaagctgt tcatgcagac acgcagagac agggtcatgc
     6841 tcagcataaa gaagcttctt aaaatggaaa accctcgttt ccttccatcc tcaccaagag
     6901 aggttcccac catcgctatt ctgggctctg gaggaggttt tcgtgcaatg gtgggcttct
     6961 ctggtgtgat gaaggctttg tatgaatctg gcgtgtttga ctgtgcaaca tatgtggcag
     7021 gactttcagg atcaacatgg tatatgtcta tgctgtactc tcatccagag tttcctgcta
     7081 aaggcccagg cgacattaat aaagagctca tgaacagagt cagtaataac ccactcaaac
     7141 tgctcctgcc ccaaaacatc aaccgctacg tcaaagcact gtggaagaag aaatctgctg
     7201 gacagccggt caccttcact gacatcttcg ggatgttgat aggagagacg cttattccag
     7261 ggagaatgaa cattaagctg agctcattga aagggaaaat taatgaaggt cagagccctc
     7321 ttcctctctt cacctgtctg cacgtcaaac ctgacgtctc tgagctcatg tttgcagatt
     7381 gggtggagtt cagcccttat gaaattggca tggccaaata tggcaccttc atgtctcctg
     7441 ggctgtttgg cagcaaattc ttcatgggaa gtgttgtgaa acaatatgag gaaaacccct
     7501 tacatttcct gatgggtgtg tggggaagtg ctttttccat cctctttaac cgggttttgg
     7561 gtgtgaagga gacgaccagc agtagcacta tggaggagga gctggagcaa attaaaccag
     7621 agcacattgt gggtgacgat tctgctgata acgaggagga aactcaaaga ggaggcacag
     7681 agtctgcaga tgcagaggac gagcgccagc gtcatgccca ggccagctgg gtacagcgca
     7741 tgttgacctc catcatgggc gacaccactc tgttcaccac tcgtgagggc cgcgcaggca
     7801 aagtgcacaa cttcatgctg ggactgaacc tgaactcaac cctgcccttc tctcccttca
     7861 gcggcatcac acaccagacc tcgctggagg aggaagtgga cgctgttact gatccggatg
     7921 agtttgagcg aatctatgag cctctggatg tgaagagcaa gaaaatccat gtggtggaca
     7981 gtggcttgac ctttaacctg ccctaccctc tcattctgcg gtgtcagaga ggagtcgacc
     8041 tgattatttc attcgacttc tcagcccgac ccagtgactc cagcccaccg ttcaaagagt
     8101 tactgctggc tgaaaaatgg gcccgtatga ataagctgcc cttcccgaag attgattcga
     8161 aagtattcga cagagagggt ctaaaagagt gctatgtgtt caagcctgct aaaggagaca
     8221 agaactgccc caccatcatt cattttgtct tggccaacat caacttccgg aattttaaag
     8281 cccctggagt tcctcgagat tcagacaagg acatagagtt tggtgacttt gacatatttg
     8341 acgagccagc ctccccttac tccactttca acttcaaata caacaaccag gccttcaaga
     8401 gacttcatga cctcatggag ttcaatacgc ttaacaacat cgaggttatc aaagaagcca
     8461 ttaaagacag cattctgctg agacgagaga acccagcgcg atgttcggtt tccctttcgc
     8521 tcagtgaaat cgaaaacaag aagttcctga aacgagacaa cagcattgca aaacgaccca
     8581 ccggagctgg aggaattcgt cctggatcta tggtgtctaa gggcgaagag ctgattaagg
     8641 agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca
     8701 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggccgtcg
     8761 agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca
     8821 aaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccctgagg
     8881 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg
     8941 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc
     9001 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggcctcc accgagaccc
     9061 tgtaccccgc tgacggcggc ctggaaggca gagccgacat ggccctgaag ctcgtgggcg
     9121 ggggccacct gatctgcaac ttgaagacca catacagatc caagaaaccc gctaagaacc
     9181 tcaagatgcc cggcgtctac tatgtggaca gaagactgga aagaatcaag gaggccgaca
     9241 aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca
     9301 aactggggca caaactgtaa ctcgagaccc agctttcttg tacaaagtgg gggatccaga
     9361 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     9421 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     9481 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     9541 ggttttttcc aacttta
//
