LOCUS       sequence-487177-        8611 bp    DNA     circular SYN 25-SEP-2025
DEFINITION  Expresses membrane-anchored transcription factor (LexA-VP16) in 
            yeast, along with YFP reporter under LexO promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Roh H, Kim D, Kim B, Jeon Y, Kim Y, Jacko M, Xu F, Lin C, Won Um J, 
            Ting AY
  TITLE     Light-activated tetanus neurotoxin for conditional proteolysis and 
            inducible synaptic inhibition in vivo
  JOURNAL   bioRxiv 2025.01.27.635161; doi: 10.1101/2025.01.27.635161
REFERENCE   2  (bases 1 to 8611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.01.27.635161; doi: 10.1101/2025.01.27.635161"
COMMENT     Sequence Label: pAct1-LexA-VP16-VAMP2 + LexO-YFP
FEATURES             Location/Qualifiers
     source          1..8611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             508..1170
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     CDS             1925..2641
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MRSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             2732..3532
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="SKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELLE
                     LVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQY
                     SACVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGEY
                     TKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDDV
                     VSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      3561..3810
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(3577..3595)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      complement(3811..4169)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3851..3872)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4063..4082
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4295..4317
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4309..4326
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4310..4326
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4333..4352
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4333..4351
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4996..5247,5248..5601)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     5539..5560
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             5788..6021
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(6288..6304)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6289..6305)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(6351..6369)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6390..6406)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6390..6406)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6403..6425)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6414..6430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6438..6444,6445..6462,6463..6468))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6483..6504
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6621..6638)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6792..7380)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6872..6891)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7551..8342,8343..8411))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8174..8193
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8412..8516)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8584..8602
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagaggca
      181 ccatatgcgt tttaagagct tggtgagcgc taggagtcac tgccaggtat cgtttgaaca
      241 cggcattagt cagggaagtc ataacacagt cctttcccgc aattttcttt ttctattact
      301 cttggcctcc tctagtacac tctatatttt tttatgcctc ttaattaagg taatgatttt
      361 catttttttt tttccaccta gcggatgact cttttttttt cttagcgatt ggcattatca
      421 cataatgaat tatacattat ataaagtaat gtgatttctt cgaagaatat actaaaaaat
      481 gagcaggcaa gataaacgaa ggcaaagatg acagagcaga aagccctagt aaagcgtatt
      541 acaaatgaaa ccaagattca gattgcgatc tctttaaagg gtggtcccct agcgatagag
      601 cactcgatct tcccagaaaa agaggcagaa gcagtagcag aacaggccac acaatcgcaa
      661 gtgattaacg tccacacagg tatagggttt ctggaccaca tgatacatgc tctggccaag
      721 cattccggct ggtcgctaat cgttgagtgc attggtgact tacacataga cgaccatcac
      781 accactgaag actgcgggat tgctctcggt caggctttta aagaggccct aggggccgtg
      841 cgtggagtaa aaaggtttgg atcaggattt gcgcctttgg atgaggcact ttccagagcg
      901 gtggtagatc tttcgaacag gccgtacgca gttgtcgaac ttggtttgca aagggagaaa
      961 gtaggagatc tctcttgcga gatgatcccg cattttcttg aaagttttgc agaggctagc
     1021 agaattaccc tccacgttga ttgtctgcga ggcaagaatg atcatcaccg tagtgagagt
     1081 gcgttcaagg ctcttgcggt tgccataaga gaagccacct cgcccaatgg aaccaacgat
     1141 gttccctcca ccaaaggtgt tcttatgtag tgacaccgat tatttaaagc tgcagcatac
     1201 gatatatata catgtgtata tatgtatacc tatgaatgtc agtaagtatg tatacgaaca
     1261 gtatgatact gaagatgaca aggtaatgca tcattctata cgtgtcattc tgaacgaggc
     1321 gcgctttcct tttttctttt tgctttttct ttttttttct cttgaactcg acggatcata
     1381 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag
     1441 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     1501 ataggccgaa atcggcaaaa tcccttagcg gccgcaataa tatataaacc tgtataatat
     1561 aaccttgaag actatatttc ttttcgagct ccctaggtgc tgtatatact cacagcataa
     1621 ctgtatatac acccagggtc taggtgctgt atatactcac agcataactg tatatacacc
     1681 cagggtctag gtgctgtata tactcacagc ataactgtat atacacccag ggtctaggtg
     1741 ctgtatatac tcacagcata actgtatata cacccagggt ctagagcatg tgctctgtat
     1801 gtatataaaa ctcttgtttt cttcttttct cttctttcct tatacattag gacctttgca
     1861 gcataaatta ctatacttct atactagtgg atcccccggg ctgcaggaat tcgatatcaa
     1921 gcttatgaga tctaaaggtg aagaattatt cactggtgtt gtcccaattt tggttgaatt
     1981 agatggtgat gttaatggtc acaaattttc tgtctccggt gaaggtgaag gtgatgctac
     2041 ttacggtaaa ttgaccttaa aatttatttg tactactggt aaattgccag ttccatggcc
     2101 aaccttagtc actactttag gttatggttt gatgtgtttt gctagatacc cagatcatat
     2161 gaaacaacat gactttttca agtctgccat gccagaaggt tatgttcaag aaagaactat
     2221 ttttttcaaa gatgacggta actacaagac cagagctgaa gtcaagtttg aaggtgatac
     2281 cttagttaat agaatcgaat taaaaggtat tgattttaaa gaagatggta acattttagg
     2341 tcacaaattg gaatacaact ataactctca caatgtttac atcatggctg acaaacaaaa
     2401 gaatggtatc aaagttaact tcaaaattag acacaacatt gaagatggtt ctgttcaatt
     2461 agctgaccat tatcaacaaa atactccaat tggtgatggt ccagtcttgt taccagacaa
     2521 ccattactta tcctatcaat ctaagttatc caaagatcca aacgaaaaga gagaccacat
     2581 ggtcttgtta gaatttgtta ctgctgctgg tattacccat ggtatggatg aattgtacaa
     2641 aggttccacg tcaggttctg gcaagcccgg atcaggcgag ggtagcggct ctacttctgg
     2701 gtctggtaag cctgggagtg gagaggggag ctcgaaagct acatataagg aacgtgctgc
     2761 tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa agcaaacaaa
     2821 cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag ttgaagcatt
     2881 aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt tttccatgga
     2941 gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac tcttcgaaga
     3001 cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgt gtgtatacag
     3061 aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag gtattgttag
     3121 cggtttgaag caggcggcgg aagaagtaac aaaggaacct agaggccttt tgatgttagc
     3181 agaattgtca tgcaagggct ccctagctac tggagaatat actaagggta ctgttgacat
     3241 tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca tgggtggaag
     3301 agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg acaagggaga
     3361 cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat ctgacattat
     3421 tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta
     3481 cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact aatagacagg
     3541 ccccttttcc tttgtctata tcatgtaatt agttatgtca cgcttacatt cacgccctcc
     3601 tcccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat
     3661 ttattttttt taatagttat gttagtatta agaacgttat ttatatttca aatttttctt
     3721 ttttttctgt acaaacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag
     3781 gttttgggac gctcgaaggc tttaatttgc taaatcaaaa gaatagaccg agatagggtt
     3841 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     3901 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     3961 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     4021 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     4081 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     4141 cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg
     4201 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     4261 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     4321 gccagtgaat tgtaatacga ctcactatag ggcgaattgg agaaacagcg gccgcctata
     4381 gggcgaattg gagctccacc gcggtggcgg ccgctctaga gaagcgggta agctgccaca
     4441 gcaattaatg cacaacattt aacctacatt cttccttatc ggatcctcaa aacccttaaa
     4501 aacatatgcc tcaccctaac atattttcca attaaccctc aatatttctc tgtcacccgg
     4561 cctctatttt ccattttctt ctttacccgc cacgcgtttt tttctttcaa atttttttct
     4621 tccttcttct ttttcttcca cgtcctcttg cataaataaa taaaccgttt tgaaaccaaa
     4681 ctcgcctctc tctctccttt ttgaaatatt tttgggtttg tttgatcctt tccttcccaa
     4741 tctctcttgt ttaatatata ttcatttata tcacgctctc tttttatctt cctttttttc
     4801 ctctctcttg tattcttcct tcccctttct actcaaacca agaagaaaaa gaaaaggtca
     4861 atctttgtta aagaatagga tcttctacta catcagcttt tagatttttc acgcttactg
     4921 cttttttctt cccaagatcg aaaatttact gaattaacaa ctagtggatc ccccgggctg
     4981 caggaattcg gtaccatgaa agcgttaacg gccaggcaac aagaggtgtt tgatctcatc
     5041 cgtgatcaca tcagccagac aggtatgccg ccgacgcgtg cggaaatcgc gcagcgtttg
     5101 gggttccgtt ccccaaacgc ggctgaagaa catctgaagg cgctggcacg caaaggcgtt
     5161 attgaaattg tttccggcgc atcacgcggg attcgtctgt tgcaggaaga ggaagaaggg
     5221 ttgccgctgg taggtcgtgt ggctgccggt gaaccacttc tggcgcaaca gcatattgaa
     5281 ggtcattatc aggtcgatcc ttccttattc aagccgaatg ctgatttcct gctgcgcgtc
     5341 agcgggatgt cgatgaaaga catcggcatt atggatggtg acttgctggc agtgcataaa
     5401 actcaggatg tacgtaacgg tcaggtcgtt gtcgcacgta ttgatgacga ggttaccgtt
     5461 aagcgcctga aaaaacaggg caataaagtc gaactgttgc cagaaaatag cgagtttaaa
     5521 ccaattgtcg tagatcttcg tcagcagagc ttcaccattg aagggctggc ggttggggtt
     5581 attcgcaacg gcgactggct gggtgatatc atgcaggatc tgccgggcaa cgataacagc
     5641 accgcgggcg cgcgtacgaa aaacaattac gggtctacca tcgagggcct gctcgatctc
     5701 ccggacgacg acgcccccga agaggcgggg ctggcggctc cgcgcctgtc ctttctcccc
     5761 gcgggacaca cgcgcagact gtcgacggcc cccccgaccg atgtcagcct gggggacgaa
     5821 ttgcacttag acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat
     5881 ctggacatgt tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc
     5941 ccctacggcg ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt
     6001 ggaattgacg agtacggtgg gaagctcgct agcgttaaca ccggtgatga ggtggtggac
     6061 atcatgaggg tgaatgtgga caaggtcctg gagcgggacc agaagttgtc ggagctggat
     6121 gaccgtgcag atgccctcca ggcaggggcc tcccagtttg aaacaagtgc agccaagctc
     6181 aagcgcaaat actggtggaa aaacctcaag atgatgatca tcttgggagt gatctgcgcc
     6241 atcatcctca tcatcatcat cgtttacttc agcacttaaa agcttatcga taccgtcgac
     6301 ctcgaggggg ggcccggtac ccagcttcgg ccgtgtacca gcttttgttc cctttagtga
     6361 gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6421 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6481 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6541 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6601 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6661 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6721 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6781 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6841 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6901 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6961 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7021 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7081 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7141 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7201 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7261 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7321 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7381 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7441 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7501 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7561 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7621 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7681 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7741 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7801 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7861 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7921 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7981 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8041 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8101 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8161 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8221 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8281 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8341 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8401 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8461 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8521 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     8581 aaaaataggc gtatcacgag gccctttcgt c
//
