LOCUS       sequence-487188-       14710 bp    DNA     circular SYN 25-SEP-2025
DEFINITION  Expression of human Parkin fused to mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14710)
  AUTHORS   Tait SW, Oberst A, Quarato G, Milasta S, Haller M, Wang R, Karvela 
            M, Ichim G, Yatim N, Albert ML, Kidd G, Wakefield R, Frase S, 
            Krautwald S, Linkermann A, Green DR
  TITLE     Widespread mitochondrial depletion via mitophagy does not compromise
            necroptosis.
  JOURNAL   Cell Rep. 2013 Nov 27;5(4):878-85. doi: 
            10.1016/j.celrep.2013.10.034. Epub 2013 Nov 21.
  PUBMED    24268776
REFERENCE   2  (bases 1 to 14710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.10.034"; journalName: "Cell Rep"; date: 
            "2013-11-27- 27"; volume: "5"; issue: "4"; pages: "878-85"
COMMENT     Sequence Label: mCherry-Parkin-IRES-zeo
FEATURES             Location/Qualifiers
     source          1..14710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..892
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             957..1373
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1303..1325
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1341..1357
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1383..1757
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1695..1711
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             1781..2488
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1926..1944)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2194..2213
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(3975..4549,4550..4552,4553..4561)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4142..4159)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4369..4388
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4562..4936
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             5159..5628
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             5893..6084
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(6078..6100)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6186..6326
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6241..6260
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6341..6358)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6512..7100)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6592..6611)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7223..7357
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7247..7266)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7301..7320
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7587..8186)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7670..7690)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8167..8186
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8269..8768)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8317..8336)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     8710..8733
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(join(13209..14000,14001..14069))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13832..13851
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14070..14174)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      721 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      781 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      841 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
      901 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc
      961 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1021 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1081 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1141 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1201 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1261 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1321 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1381 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     1441 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     1501 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1561 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1621 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1681 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1741 gaccatcctc tagactgccg gatctaagta agtaggatcc atggtgagca agggcgagga
     1801 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     1861 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     1921 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     1981 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     2041 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     2101 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     2161 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     2221 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     2281 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     2341 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     2401 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     2461 caccggcggc atggacgagc tgtacaagtc cggactcaga tctcgagctc aagctttgat
     2521 agtgtttgtc aggttcaact ccagccatgg tttcccagtg gaggtcgatt ctgacaccag
     2581 catcttccag ctcaaggagg tggttgctaa gcgacagggg gttccggctg accagttgcg
     2641 tgtgattttc gcagggaagg agctgaggaa tgactggact gtgcagaatt gtgacctgga
     2701 tcagcagagc attgttcaca ttgtgcagag accgtggaga aaaggtcaag aaatgaatgc
     2761 aactggaggc gacgacccca gaaacgcggc gggaggctgt gagcgggagc cccagagctt
     2821 gactcgggtg gacctcagca gctcagtcct cccaggagac tctgtggggc tggctgtcat
     2881 tctgcacact gacagcagga aggactcacc accagctgga agtccagcag gtagatcaat
     2941 ctacaacagc ttttatgtgt attgcaaagg cccctgtcaa agagtgcagc cgggaaaact
     3001 cagggtacag tgcagcacct gcaggcaggc aacgctcacc ttgacccagg gtccatcttg
     3061 ctgggatgat gttttaattc caaaccggat gagtggtgaa tgccaatccc cacactgccc
     3121 tgggactagt gcagaatttt tctttaaatg tggagcacac cccacctctg acaaggaaac
     3181 atcagtagct ttgcacctga tcgcaacaaa tagtcggaac atcacttgca ttacgtgcac
     3241 agacgtcagg agccccgtcc tggttttcca gtgcaactcc cgccacgtga tttgcttaga
     3301 ctgtttccac ttatactgtg tgacaagact caatgatcgg cagtttgtcc acgaccctca
     3361 gcttggctac tccctgcctt gtgtggctgg ctgtcccaac tccttgatta aagagctcca
     3421 tcacttcagg attctgggag aagagcagta caaccggtac cagcagtatg gtgcagagga
     3481 gtgtgtcctg cagatggggg gcgtgttatg cccccgccct ggctgtggag cggggctgct
     3541 gccggagcct gaccagagga aagtcacctg cgaagggggc aatggcctgg gctgtgggtt
     3601 tgccttctgc cgggaatgta aagaagcgta ccatgaaggg gagtgcagtg ccgtatttga
     3661 agcctcagga acaactactc aggcctacag agtcgatgaa agagccgccg agcaggctcg
     3721 ttgggaagca gcctccaaag aaaccatcaa gaaaaccacc aagccctgtc cccgctgcca
     3781 tgtaccagtg gaaaaaaatg gaggctgcat gcacatgaag tgtccgcagc cccagtgcag
     3841 gctcgagtgg tgctggaact gtggctgcga gtggaaccgc gtctgcatgg gggaccactg
     3901 gttcgacgtg taggaattcc tgcaggcctc gagggccggc gcgccgcggc cgctacgtaa
     3961 attccgcccc cccccccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
     4021 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     4081 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     4141 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     4201 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     4261 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     4321 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     4381 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     4441 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     4501 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac
     4561 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     4621 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     4681 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     4741 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     4801 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     4861 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     4921 cgaggagcag gactgaccga cgccgaccaa caccgccggt ccgacgcggc ccgacgggtc
     4981 cgaggggggt cgacgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
     5041 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
     5101 aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga tggaacagct
     5161 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     5221 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     5281 ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag
     5341 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     5401 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     5461 aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta
     5521 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
     5581 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcaca tgcagcatgt
     5641 atcaaaatta gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta
     5701 acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc
     5761 agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc
     5821 gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac
     5881 acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga
     5941 agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac
     6001 atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc
     6061 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     6121 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     6181 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     6241 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     6301 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     6361 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6421 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6481 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6541 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6601 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6661 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6721 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6781 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6841 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6901 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     6961 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7021 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7081 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7141 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7201 agatcctttt gcggccgaat tagatccaga catgataaga tacattgatg agtttggaca
     7261 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     7321 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     7381 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     7441 atgtggtatg gctgattatg atcctctaga gtcggtgggc ctcgggggcg ggtgcggggt
     7501 cggcggggcc gccccgggtg gcttcggtcg gagccatggg gtcgtgcgct cctttcggtc
     7561 gggcgctgcg ggtcgtgggg cgggcgtcag gcaccgggct tgcgggtcat gcaccaggtg
     7621 cgcggtcctt cgggcacctc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag
     7681 gggaggttgc ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc
     7741 tccactccgg ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg
     7801 ccgacggtgg ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc
     7861 atctgttgct gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg
     7921 gcgaacaccg cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg
     7981 tcgtccgcga cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc
     8041 agctcggtga cccgctcgat gtggcggtcc gggtcgacgg tgtggcgcgt ggcggggtag
     8101 tcggcgaacg cggcggcgag ggtgcgtacg gcccggggga cgtcgtcgcg ggtggcgagg
     8161 cgcaccgtgg gcttgtactc ggtcatggaa ggtcgtctcc ttgtgagggg tcaggggcgt
     8221 gggtcagggg atggtggcgg caccggtcgt ggcggccgac ctgcaggtcg aaaggcccgg
     8281 agatgaggaa gaggagaaca gcgcggcaga cgtgcgcttt tgaagcgtgc agaatgccgg
     8341 gcctccggag gaccttcggg cgcccgcccc gcccctgagc ccgcccctga gcccgccccc
     8401 ggacccaccc cttcccagcc tctgagccca gaaagcgaag gagcaaagct gctattggcc
     8461 gctgccccaa aggcctaccc gcttccattg ctcagcggtg ctgtccatct gcacgagact
     8521 agtgagacgt gctacttcca tttgtcacgt cctgcacgac gcgagctgcg gggcgggggg
     8581 gaacttcctg actaggggag gagtagaagg tggcgcgaag gggccaccaa agaacggagc
     8641 cggttggcgc ctaccggtgg atgtggaatg tgtgcgaggc cagaggccac ttgtgtagcg
     8701 ccaagtgccc agcggggctg ctaaagcgca tgctccagac tgccttggga aaagcgcctc
     8761 ccctacccgg tagaattaat tctcatgttt gacagcttat catcgataag ctgatcctca
     8821 caggccgcac ccagcttttc ttccgttgcc ccagtagcat ctctgtctgg tgaccttgaa
     8881 gaggaagagg aggggtcccg agaatcccca tccctaccgt ccagcaaaaa gggggacgag
     8941 gaatttgagg cctggcttga ggctcaggac gcaaatcttg aggatgttca gcgggagttt
     9001 tccgggctgc gagtaattgg tgatgaggac gaggatggtt cggaggatgg ggaattttca
     9061 gacctggatc tgtctgacag cgaccatgaa ggggatgagg gtgggggggc tgttggaggg
     9121 ggcaggagtc tgcactccct gtattcactg agcgtcgtct aataaagatg tctattgatc
     9181 tcttttagtg tgaatcatgt ctgacgaggg gccaggtaca ggacctggaa atggcctagg
     9241 agagaaggga gacacatctg gaccagaagg ctccggcggc agtggacctc aaagaagagg
     9301 gggtgataac catggacgag gacggggaag aggacgagga cgaggaggcg gaagaccagg
     9361 agccccgggc ggctcaggat cagggccaag acatagagat ggtgtccgga gaccccaaaa
     9421 acgtccaagt tgcattggct gcaaagggac ccacggtgga acaggagcag gagcaggagc
     9481 gggaggggca ggagcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc
     9541 aggaggggca ggaggggcag gagcaggagg agggggagga ggggcaggag caggaggagg
     9601 gggaggaggg gcaggaggag gaggaggggg aggaggagga ggagggggag gaggggcagg
     9661 aggaggagga gggggaggag ggggaggagg aggagggtga ggtgcaggat taagagatac
     9721 gggcccaggg gggagccctg catgttggtt ggcgactgct gtatgtggaa gttgtgggca
     9781 gccacagcgc gcatcaatca cgatgacaca ctctatcata tggtgttttc ttgatatgtc
     9841 tgacgcactc acggcatcga tatggttcat agtcgtgggc cgcgtcaggt ttgagcagga
     9901 ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggggcagg aggggcagga
     9961 gcaggaggag gggcaggagc aggaggggca ggagcaggag gtggaggccg gggtcgagga
    10021 ggcagtggag gccggggtcg aggaggtagt ggaggccggg gtcgaggagg tagtggaggc
    10081 cgccggggta gaggacgtga aagagccagg gggggaagtc gtgaaagagc cagggggaga
    10141 ggtcgtggac gtggagaaaa gaggcccagg agtcccagta gtcagtcatc atcatccggg
    10201 tctccaccgc gcaggccccc tccaggtaga aggccatttt tccaccctgt aggggaagcc
    10261 gattattttg aataccacca agaaggtggc ccagatggtg agcctgacgt gcccccggga
    10321 gcgatagagc agggccccgc agatgaccca ggagaaggcc caagcactgg accccggggt
    10381 cagggtgatg gaggcaggcg caaaaaagga gggtggtttg gaaagcatcg tggtcaagga
    10441 ggttccaacc cgaaatttga gaacattgca gaaggtttaa gagctctcct ggctaggagt
    10501 cacgtagaaa ggactaccga cgaaggaact tgggtcgccg gtgtgttcgt atatggaggt
    10561 agtaagacct ccctttacaa cctaaggcga ggaactgccc ttgctattcc acaatgtcgt
    10621 cttacaccat tgagtcgtct cccctttgga atggcccctg gacccggccc acaacctggc
    10681 ccgctaaggg agtccattgt ctgttatttc atggtctttt tacaaactca tatatttgct
    10741 gaggttttga aggatgcgat taaggacctt gttatgacaa agcccgctcc tacctgcaat
    10801 atcagggtga ctgtgtgcag ctttgacgat ggagtagatt tgcctccctg gtttccacct
    10861 atggtggaag gggctgccgc ggagggtgat gacggagatg acggagatga aggaggtgat
    10921 ggagatgagg gtgaggaagg gcaggagtga tgtaacttgt taggagacgc cctcaatcgt
    10981 attaaaagcc gtgtattccc ccgcactaaa gaataaatcc ccagtagaca tcatgcgtgc
    11041 tgttggtgta tttctggcca tctgtcttgt caccattttc gtcctcccaa catggggcaa
    11101 ttgggcatac ccatgttgtc acgtcactca gctccgcgct caacaccttc tcgcgttgga
    11161 aaacattagc gacatttacc tggtgagcaa tcagacatgc gacggcttta gcctggcctc
    11221 cttaaattca cctaagaatg ggagcaacca gcaggaaaag gacaagcagc gaaaattcac
    11281 gcccccttgg gaggtggcgg catatgcaaa ggatagcact cccactctac tactgggtat
    11341 catatgctga ctgtatatgc atgaggatag catatgctac ccggatacag attaggatag
    11401 catatactac ccagatatag attaggatag catatgctac ccagatatag attaggatag
    11461 cctatgctac ccagatataa attaggatag catatactac ccagatatag attaggatag
    11521 catatgctac ccagatatag attaggatag cctatgctac ccagatatag attaggatag
    11581 catatgctat ccagatattt gggtagtata tgctacccat ggcaacatta gcccaccgtg
    11641 ctctcagcga cctcgtgaat atgaggacca acaaccctgt gcttggcgct caggcgcaag
    11701 tgtgtgtaat ttgtcctcca gatcgcagca atcgcgcccc tatcttggcc cgcccaccta
    11761 cttatgcagg tattccccgg ggtgccatta gtggttttgt gggcaagtgg tttgaccgca
    11821 gtggttagcg gggttacaat cagccaagtt attacaccct tattttacag tccaaaaccg
    11881 cagggcggcg tgtgggggct gacgcgtgcc cccactccac aatttcaaaa aaaagagtgg
    11941 ccacttgtct ttgtttatgg gccccattgg cgtggagccc cgtttaattt tcgggggtgt
    12001 tagagacaac cagtggagtc cgctgctgtc ggcgtccact ctctttcccc ttgttacaaa
    12061 tagagtgtaa caacatggtt cacctgtctt ggtccctgcc tgggacacat cttaataacc
    12121 ccagtatcat attgcactag gattatgtgt tgcccatagc cataaattcg tgtgagatgg
    12181 acatccagtc tttacggctt gtccccaccc catggatttc tattgttaaa gatattcaga
    12241 atgtttcatt cctacactag tatttattgc ccaaggggtt tgtgagggtt atattggtgt
    12301 catagcacaa tgccaccact gaaccccccg tccaaatttt attctggggg cgtcacctga
    12361 aaccttgttt tcgagcacct cacatacacc ttactgttca caactcagca gttattctat
    12421 tagctaaacg aaggagaatg aagaagcagg cgaagattca ggagagttca ctgcccgctc
    12481 cttgatcttc agccactgcc cttgtgacta aaatggttca ctaccctcgt ggaatcctga
    12541 ccccatgtaa ataaaaccgt gacagctcat ggggtgggag atatcgctgt tccttaggac
    12601 ccttttacta accctaattc gatagcatat gcttcccgtt gggtaacata tgctattgaa
    12661 ttagggttag tctggatagt atatactact acccgggaag catatgctac ccgtttaggg
    12721 ttaacaaggg ggccttataa acactattgc taatgccctc ttgagggtcc gcttatcggt
    12781 agctacacag gcccctctga ttgacgttgg tgtagcctcc cgtagtcttc ctgggcccct
    12841 gggaggtaca tgtcccccag cattggtgta agagcttcag ccaagagtta cacataaagg
    12901 caatgttgtg ttgcagtcca cagactgcaa agtctgctcc aggatgaaag ccactcagtg
    12961 ttggcaaatg tgcacatcca tttataagga tgtcaactac agtcagagaa cccctttgtg
    13021 tttggtcccc ccccgtgtca catgtggaac agggcccagt tggcaagttg taccaaccaa
    13081 ctgaagggat tacatgcact gccccgcgaa gaaggggcag agatgtcgta gtcaggttta
    13141 gttcgtccgg ggcggggatc tggccgcaaa tcaatctaaa gtatatatga gtaaacttgg
    13201 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    13261 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    13321 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    13381 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    13441 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    13501 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    13561 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    13621 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    13681 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    13741 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    13801 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    13861 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    13921 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    13981 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    14041 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    14101 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    14161 ataggggttc cgcgcacatt tccccgaaaa gtgccaccag ctttgctctt aggagtttcc
    14221 taatacatcc caaactcaaa tatataaagc atttgacttg ttctatgccc tagggggcgg
    14281 ggggaagcta agccagcttt ttttaacatt taaaatgtta attccatttt aaatgcacag
    14341 atgtttttat ttcataaggg tttcaatgtg catgaatgct gcaatattcc tgttaccaaa
    14401 gctagtataa ataaaaatag ataaacgtgg aaattactta gagtttctgt cattaacgtt
    14461 tccttcctca gttgacaaca taaatgcgct gctgagaagc cagtttgcat ctgtcaggat
    14521 caatttccca ttatgccagt catattaatt actagtcaat tagttgattt ttatttttga
    14581 catatacatg tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
    14641 tgcaaggcat ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca
    14701 gatggaacag
//
