LOCUS       sequence-487268-       11083 bp    DNA     circular SYN 26-SEP-2025
DEFINITION  Expresses plant cytochrome P450 in yeast for enzyme assay. Contains 
            CPR1 from Lotus japonicus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11083)
  AUTHORS   Khatri P, Chen L, Rajcan I, Dhaubhadel S
  TITLE     Functional characterization of Cinnamate 4-hydroxylase gene family 
            in soybean (Glycine max).
  JOURNAL   PLoS One. 2023 May 15;18(5):e0285698. doi: 
            10.1371/journal.pone.0285698. eCollection 2023.
  PUBMED    37186600
REFERENCE   2  (bases 1 to 11083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2023-05-15"; volume: "18(5):e0285698. doi"; pages: " 
            10.1371/journal.pone.0285698. eCollection 2023"
COMMENT     Sequence Label: pESC-leu2D-LjCPR1-GW
FEATURES             Location/Qualifiers
     source          1..11083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(157..1251)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     rep_origin      complement(1559..2014)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1696..1717)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     terminator      complement(2093..2258)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     2179..2203
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        4560..5224
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    4776..4893
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     5188..5211
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     5235..5254
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5235..5253
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5262..5386
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5411..5416,5417..5434,5435..5441)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5495..6154
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5561..5580)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6496..6801
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6680..6699
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6842..6966)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      6983..7172
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(7244..7261)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7415..8003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7495..7514)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8174..8965,8966..9034))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8797..8816
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9035..9139)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9166..10508
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10129..10176)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10549..10567
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10605..10627)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10727..10746
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atcctccttt ttctccttct tgataaatgt atgtagattg cgtatatagt ttcgtctacc
       61 ctatgaacat attccatttt gtaatttcgt gtcgtttcta ttatgaattt catttataaa
      121 gtttatgtac aaatatcata aaaaaagaga atctttttaa gcaaggattt tcttaacttc
      181 ttcggcgaca gcatcaccga cttcggtggt actgttggaa ccacctaaat caccagttct
      241 gatacctgca tccaaaacct ttttaactgc atcttcaatg gccttacctt cttcaggcaa
      301 gttcaatgac aatttcaaca tcattgcagc agacaagata gtggcgatag ggttgacctt
      361 attctttggc aaatctggag cagaaccgtg gcatggttcg tacaaaccaa atgcggtgtt
      421 cttgtctggc aaagaggcca aggacgcaga tggcaacaaa cccaaggaac ctgggataac
      481 ggaggcttca tcggagatga tatcaccaaa catgttgctg gtgattataa taccatttag
      541 gtgggttggg ttcttaacta ggatcatggc ggcagaatca atcaattgat gttgaacctt
      601 caatgtaggg aattcgttct tgatggtttc ctccacagtt tttctccata atcttgaaga
      661 ggccaaaaca ttagctttat ccaaggacca aataggcaat ggtggctcat gttgtagggc
      721 catgaaagcg gccattcttg tgattctttg cacttctgga acggtgtatt gttcactatc
      781 ccaagcgaca ccatcaccat cgtcttcctt tctcttacca aagtaaatac ctcccactaa
      841 ttctctgaca acaacgaagt cagtaccttt agcaaattgt ggcttgattg gagataagtc
      901 taaaagagag tcggatgcaa agttacatgg tcttaagttg gcgtacaatt gaagttcttt
      961 acggattttt agtaaacctt gttcaggtct aacactaccg gtaccccatt taggaccacc
     1021 cacagcacct aacaaaacgg catcagcctt cttggaggct tccagcgcct catctggaag
     1081 tggaacacct gtagcatcga tagcagcacc accaattaaa tgattttcga aatcgaactt
     1141 gacattggaa cgaacatcag aaatagcttt aagaacctta atggcttcgg ctgtgatttc
     1201 ttgaccaacg tggtcacctg gcaaaacgac gatcttctta ggggcagaca ttacaatggt
     1261 atatccttga aatatatata aaaaaaggcg ccttagaccg ctcggccaaa caaccaatta
     1321 cttgttgaga aatagagtat aattatccta taaatataac gtttttgaac acacatgaac
     1381 aaggaagtac aggacaattg attttgaaga gaatgtggat tttgatgtaa ttgttgggat
     1441 tccattttta ataaggcaat aatattaggt atgtagatat actagaagtt ctcctcgacc
     1501 ggtcgatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa
     1561 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     1621 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     1681 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     1741 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     1801 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     1861 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     1921 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     1981 cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact
     2041 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctgaat tggagcgacc
     2101 tcatgctata cctgagaaag caacctgacc tacaggaaag agttactcaa gaataagaat
     2161 tttcgtttta aaacctaaga gtcactttaa aatttgtata cacttatttt ttttataact
     2221 tatttaataa taaaaatcat aaatcataag aaattcgctt atttagaagt gtcaacaacg
     2281 tatctaccaa cgatttgacc cttttccatc ttttcgtaaa tttctggcaa ggtagacaag
     2341 ccgacaacct tgattggaga cttgaccaaa cctctggcga agaattgtta attaatcacc
     2401 atacatcacg caaataccta ccattcaatt gtaggttctt aaccataccc tcagccttgg
     2461 aactatcgag agaaccctgc tcttgcaaaa ttgtgtgcag agtacggtgc acatccctag
     2521 ccatgccctt ggcatcccca cacacataaa tgtaagctcc ctgagatatc atgttccaaa
     2581 tatccgaagc cttctccatc attttatgtt gcacatattc cttggtaggt ccctcccgtg
     2641 agaaggcaac aatgagctca gaaagcgcac cactgttgac aaagtggttc aactcatctt
     2701 catagatgta gtccatttga cgattcctgc atccgaagaa taaaacggag gggccaagtt
     2761 cagctccatc ctctttcaga gctaatcttt cctgcaagaa acccctgaaa ggagccaatc
     2821 ctgtgccagg acctatcatg attataggca ctttattatc agcagggagt ttaaaattgg
     2881 actgtctaac aaatattgga gcccaactgc agtcctgact tttctccaat ggcacagaat
     2941 tcttcatcca tgttgaacac actcccctat gaatcctacc agtgggcatt ttatcattca
     3001 ctaatgcaca ggtaacgtga attctggatg gagccatcct aggagatgat gagatcgaat
     3061 aaaatcttgg ctgcaggcga ggagcaattg ctgcgaaaaa gacaccaatt ggaggtttgg
     3121 ctgatggaaa ttcagccatg acctcaagga gacttctttg actggcaatc acccactctg
     3181 aatattcatc ctttccagca ggtgaagcaa gatgtcgtag gcggtcggct tcagatggat
     3241 tagatgcatg agcagctaag gcaagcaaaa cagacttttt gggtgaactc aagacatctg
     3301 catatcgggc aattgctgtt cttaaagtac atggtgggaa agtaggtggc aaggagcttc
     3361 cactaagtgg tttcccatct tcatcatcag tatggacgga gaaataggta tctggtgaca
     3421 aacctagtaa ccttactgct tcttccacag tttcagataa attctcacag taaacaccaa
     3481 catggtcccc tgtttcatat gcaactccag tgcctgaaat gtcaaattct aaatgtgtgc
     3541 aagaacgatc tgacacagga gtatgaagct cctttcgcac agccacattt gacctgactg
     3601 gatgttgagc atccacaata gcatggccat taacattatg ccacttcttt tcatcgacag
     3661 atgcatctaa tggatcatga ataacaacac gatactccaa cacagcagcc gtatagggtg
     3721 tagacacagt tgtatcatca tcacctctaa gcaattcatc caatgctggc cacaattctt
     3781 ctttccatgc agtaaagtca tcttctatgc attgatcatc atctcctaga cctactttga
     3841 caagacgctt cccaccaaaa tcagcaagct tatcatccac ttcaatggcg accttattaa
     3901 aatgctcata ctgcctgttc cccagaccaa aaacagcata ctcaagattt cgaagccatc
     3961 cttcttcttt ctcatctccc tccagaaacc atttgtaaaa tctcgccgca ttatcagttg
     4021 gctcaccatc gccatatgta gctaagaaga aaagtgccag tgtctctttc ttgagctttt
     4081 cctcatactc atcatcgtcc tgggcataat catccatatc aacaattttg aacttggctt
     4141 tttcgtatcg cacttttgcc tcttccgcaa tcgccttggc gaatccttca gcagtaccag
     4201 tctgagtacc gaagaagatg gtgacctttc tggtaccgtc gtcaacctca gcctcgtcgc
     4261 taagcttctc gacgacgcgc ttgggaggct cgatggactt agccttgtta ccggtggatc
     4321 tgcgccaaat gaaaacgacg acgcatccga tgaggacggc gatggaggtg gtaagcacca
     4381 tgacgaactc acgattctcg aggaagacgg agccggcgcc ggaggtggag gagacgttgg
     4441 aagggtcgag tgtgcccttg atcatggcgg acatcagatc cagaggcgaa atcttcatgg
     4501 agcttgattc ttccatcgat actagtgcgg ccgcccttta gtgagggttg aattcgaatt
     4561 ttcaaaaatt cttacttttt ttttggatgg acgcaaagaa gtttaataat catattacat
     4621 ggcattacca ccatatacat atccatatac atatccatat ctaatcttac ttatatgttg
     4681 tggaaatgta aagagcccca ttatcttagc ctaaaaaaac cttctctttg gaactttcag
     4741 taatacgctt aactgctcat tgctatattg aagtacggat tagaagccgc cgagcgggtg
     4801 acagccctcc gaaggaagac tctcctccgt gcgtcctcgt cttcaccggt cgcgttcctg
     4861 aaacgcagat gtgcctcgcg ccgcactgct ccgaacaata aagattctac aatactagct
     4921 tttatggtta tgaagaggaa aaattggcag taacctggcc ccacaaacct tcaaatgaac
     4981 gaatcaaatt aacaaccata ggatgataat gcgattagtt ttttagcctt atttctgggg
     5041 taattaatca gcgaagcgat gatttttgat ctattaacag atatataaat gcaaaaactg
     5101 cataaccact ttaactaata ctttcaacat tttcggtttg tattacttct tattcaaatg
     5161 taataaaagt atcaacaaaa aattgttaat atacctctat actttaacgt caaggagaaa
     5221 aaaccccgga tccgtaatac gactcactat agggccccat cacaagtttg tacaaaaaag
     5281 ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
     5341 aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg
     5401 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     5461 tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     5521 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     5581 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     5641 aaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     5701 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     5761 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     5821 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     5881 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     5941 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     6001 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     6061 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     6121 acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc
     6181 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     6241 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt
     6301 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     6361 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     6421 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     6481 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     6541 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
     6601 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     6661 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
     6721 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     6781 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
     6841 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     6901 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
     6961 agtggtgccg cggctagcta agatccgctc taaccgaaaa ggaaggagtt agacaacctg
     7021 aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat
     7081 ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa
     7141 ccttgcttga gaaggttttg ggacgctcga agatccagct gcattaatga atcggccaac
     7201 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7261 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7321 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7381 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7441 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7501 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7561 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7621 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7681 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7741 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7801 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7861 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7921 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7981 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8041 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8101 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8161 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8221 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8281 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8341 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8401 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8461 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8521 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8581 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8641 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8701 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8761 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8821 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8881 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8941 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9001 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9061 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9121 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaacga agcatctgtg
     9181 cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg
     9241 agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca acgaagaatc
     9301 tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt caaacaaaga
     9361 atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt taccaacaaa
     9421 gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat ttttctaaca
     9481 aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc tcttgataac
     9541 tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct attttctctt
     9601 ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa gctgcgggtg
     9661 cattttttca agataaaggc atccccgatt atattctata ccgatgtgga ttgcgcatac
     9721 tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat tatgaacggt
     9781 ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc gtattgtttt
     9841 cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta atactagaga
     9901 taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga aaggtggatg
     9961 ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt ttgagcaatg
    10021 tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg cgtttttggt
    10081 tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga agttcctata
    10141 ctttctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct
    10201 tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct
    10261 gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa
    10321 atgcgtactt atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat
    10381 attatcccat tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct
    10441 atatgctgcc actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat
    10501 attggatcat actaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
    10561 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
    10621 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
    10681 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
    10741 attgtactga gagtgcacca tatcgactac gtcgttaagg ccgtttctga cagagtaaaa
    10801 ttcttgaggg aactttcacc attatgggaa atggttcaag aaggtattga cttaaactcc
    10861 atcaaatggt caggtcattg agtgtttttt atttgttgta tttttttttt tttagagaaa
    10921 atcctccaat atataaatta ggaatcatag tttcatgatt ttctgttaca cctaactttt
    10981 tgtgtggtgc cctcctcctt gtcaatatta atgttaaagt gcaattcttt ttccttatca
    11041 cgttgagcca ttagtatcaa tttgcttacc tgtattcctt tac
//
