LOCUS       sequence-487421-       14525 bp    DNA     circular SYN 29-SEP-2025
DEFINITION  Expresses Bahcc1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14525)
  AUTHORS   Monziani A, Perry RBT, Hezroni H, Ulitsky I
  TITLE     BAHCC1 promotes gene expression in neuronal cells by antagonizing 
            SIN3A-HDAC1
  JOURNAL   bioRxiv 2025.01.24.634719
REFERENCE   2  (bases 1 to 14525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 29, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.01.24.634719"
COMMENT     Sequence Label: pLIX-Bahcc1-HA_deltaBAH
FEATURES             Location/Qualifiers
     source          1..14525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7398..7424
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7440..7457
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        7467..7977
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7896..7914
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7987..8583
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7987..8006)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8483..8503
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             8584..8637
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(8638..9255,9256..9261,9262..9297,9298..9300,9301..9336,
                     9337..9339,9340..9375,9376..9384)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(9424..9444)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9842..9853)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10058..10291
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10363..10484
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10400..10419)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10454..10473
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10524..10659
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        10659..10730
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10731..10799,10800..11591)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10949..10968)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11762..12350
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12251..12270
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12504..12521
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12638..12659
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12674..12679,12680..12697,12698..12704)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12712..12728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12717..12739
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12736..12752
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12736..12752
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12771..12791
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12773..12791
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        12819..13045
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             13046..13226
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    13273..13398
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13891..14124
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             14309..14353
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatggcttc gcacacagcc agcagccgtc tgatgggaaa ccccccagcc tcctctttta
      601 tgggcagctt cctcaccagc agcctgggct cagcagcgtc ggcacacccc agtggcccca
      661 catcctctcc ctctgagccg gcctaccgag gatcccaccc tgccacctct cagatctggt
      721 tctcccactc ccatgaagct cctccgtacc ccagattttc ggggagtctg gcatctacct
      781 tcctacccgt gagccacttg gatcaccatg gaaacagcaa tgttctctat gggcaacatc
      841 gtttctatgg aacccaaaaa gataacttct acctgcgcaa cctgccccca cagcctacca
      901 tactacccgc caaccacaac ttccccggag tgccccgagc cacccctgcc catcccatcg
      961 ggtcctgcag ccgggaccgc atcgaggctg cctcactgca gaagggtcct aaggagtttg
     1021 accgcttcct catgggtaaa gaggtgggca aagagaaagt tagcaaggga gcagagggca
     1081 gggagcggcc agctgtggag gaagacagtg gtaaagatcg acagaagctg gtgccgccca
     1141 tgcctgccga ggggccctgc aaggaggcag gccctgcacc ccgggggtcc tgtgagggcc
     1201 gccccaaaca cctcacctct tgcctgctca acaccaaggt actcaatgga gacatgggca
     1261 aggcctccct ggccagctgt gcaggaggca tgctgggcag gcctgggaca ggtgtggcag
     1321 cacccggacg ctgtgccaag gaggtggcag gccccgtgga gcctgggcca gccttcagtg
     1381 agtgcctgga gcggcggcag atgctgcatc atgctgtatc ctacacagta ccctctggcc
     1441 tgcctaccgg gccacccccg cccctcagca caggcccagc gggctctttc ccctgcctgc
     1501 agctgcatgc aggcccagat gggctctgcc ctctgcagga caaagtctcc cgggacctaa
     1561 aggccagcgg gcccaccttt gtgccttctg tgggacacct ggctgacaag agccgctctt
     1621 tccaagtagc agaggcctgt gccgtggcag gtgatggaaa ggaccggcac ctggatgcgg
     1681 ccatggccac cgaccatggt gcaccctatg gagtctccta cgcccaccta aaagctgagg
     1741 gcaagggcga gcggcgaccc gggggctttg aggcggccct ccacccccgg ctgaaaggcc
     1801 tggagtatct cagttcaggc cccgaggccc ccttccctgg acttcccaaa ggcggtctgg
     1861 acaaaagtgg ctactttgag ttgcccactt cacaggactg tgcccggtcc aatcaccaag
     1921 acccactggg tgggaaggcc acccaggcct gctgcacttt agacaaagtg gccaacaaag
     1981 aggcccctgc tggccctcca gtgggacaga aggtagccag gatccggcac cagcagcact
     2041 tggtggctcc tgaggtagaa tcgggaggca gcggggccga gacgaaacgc aagtccgtgg
     2101 agctggcttc tctgggttat agcggccacc acatgcctcc gtggggtgta cagacgggcc
     2161 acgacacctc catggccatc atcgaggaac gaaagggcag tgcctacctc gacccttttg
     2221 gaagtggctt acagcaggca gccctcctgt cccaggagct gcccactccg ccagatgagg
     2281 tctcggccat gaagaacctg ctgaaataca gcaaccaagc cctagtcgtg ggccagaagg
     2341 ctccgttcgt gggcctgggc agcctcaaag ccagctgcgt ccagcaggaa gccaagtttc
     2401 cagccactaa gggcccaggc ccagtggaaa gacccgactg tgcccgcagc agggagcatg
     2461 aggccccaca tggagacggg gaggtgaggc agccacccgt gggcatcgca gtggccttgg
     2521 ccaggcagaa ggacacggtc ggcaggcccg atacagccta caataccaac agtgggcggc
     2581 agggcagggc cgcccccacc ttcaaaggtg ctggaggacc acgtgccagc catgcgctag
     2641 acctggagag cgaagaggag aggtcacggg cgtgcgagga gcgcctgggt ctgcctggac
     2701 gtgagctgct gttacaagac aacaaagacc tcgtggaatt tgcccggatc cacccttcaa
     2761 gcagctgccc tggagacctg cccccccacc tcatgatgca aggcggggac ccagcccccc
     2821 acccccatcc tgcccatccc cactggctgc cccgcacccg cagtccctcc ttgtggatgg
     2881 ggggacattc ctatggtctc ggacaccccg ccctgcacca gaacctaccc cctggcttcc
     2941 ccgcatctgt gcccggctcc atgccctccg tgttccccct cccccaagat gcagccacgc
     3001 agttggtcat cttgccctcg gaacccacac cccacaccac ccctcacaca ctcgctgaag
     3061 ttatggacca ggcctccctg tggcccccca tgtatggggc ccggggccct gcctctcaca
     3121 tgcagcaccc gggccagctc cctgtgtatt ctcggtccca gctgcttcgg cagcaagagc
     3181 tctatgcact gcagcagcag cagcaacaac agcagcagca gcaacaacag cagcaacagc
     3241 agcagcaaca acaacagcag cagcagcaac agcagcagca gcaacatcgg gccacacagg
     3301 ccctggagct acagcgagtt gcccagttcc agcgcaagcc tgaggatcgc cacatggagc
     3361 tggaggaagc tgcccaggag aagaccccaa agtccaccca caagccagtt gccttaaccc
     3421 ccatggccaa gggcaccccc tcctccgcca ctgcaggcct ggtcaagcta tcaccctgct
     3481 gtcagtcgcc cactttgaag acccctgcga gttgccccac accaccacct cggcccagcg
     3541 ccccctgcac tttacccatc tgccctactg gcagcccagg gcctggctcc aaggtgccta
     3601 gtaccatgga caagagtgag gagggccagc gggctggaac caacctgacc acgctggaac
     3661 cagacctgac tccaggattg aaccccacag ctggcctgga tctctccctc ccttcagacg
     3721 tgcactcctc tgacctccaa gaccccaaaa ctatgcaaac cactacccca gggactcggc
     3781 ctgagccccc aaggacgttc ctccctgggg agccaccccc atgcagcccc aggaacttag
     3841 aagagcctgg gctgctctca agggccaggg atgccaccca ggaccttgcc aatctacccc
     3901 ctcctgttga gggaggactc ccaccaggga aggcagaaga ccccagccca ctcgaggggt
     3961 tacaagcact gaaatttgga gacctcctcg agggaggggg aactgaggct actggccaga
     4021 cgaattctac tcagggaggg atgcaaaatg agaggactgt agatcagggg gcaccacagc
     4081 cccctttggg ggctacccct caagcactag aacaggaagc agggagccca gctgccctgg
     4141 acaagcgtga aggtccacag aaggtccctg atgtggccca gctacaggag gaagaaaccc
     4201 agcttgagga gagcgggggg gactcggagg tggactgggg gactcccaac catagccacc
     4261 cacccaaagc gctgccaggc ttggatgcct tggtggctgc tactgtggac ctaggagacc
     4321 tgcctgacat cagcctgaca gaccctcaga ccccagcagc ctccgtgccc cttagcacgg
     4381 ctcctctgcc gcatagctca gggattcatg ggattgccct gctcagtgag ctggctgacc
     4441 tggagaccca gcggcagaag agtgagctgt ccatgcaaga agatgaggat gtgctggcct
     4501 tcaacctgca gcacctggcc acactggcca cagcctggtc cctggtggag gctgctaacc
     4561 tggacagccc agtcacttca ctgcaggccc cagctgctga cccagacaga ggccccaggc
     4621 tcacccccag aatgcaaatc ctgcaacgca aggacacctg ggcccctaaa acaaagcctg
     4681 tatgtcccct gaaggctgcc atcgaccggc tggacacaca ggaagtggag atgcgcatgc
     4741 agctagcaga gctgcagagg cgctacaagg agaagcaacg ggagctggct cgcctgcagc
     4801 gcaggcatga ccacgagaga gaggagagct cacggagccc tgcacggcgg gggcctggca
     4861 ggccaagaaa gcgcaaacac tcaagctcac tgcctgccct gcgtcccggg ggccagcttg
     4921 ccagaagtga cagcaagaaa gccaaggctg ttcgtgcaag cctaagtctg ctgtgtgctg
     4981 agctacgagg tgatgagccc ccaaggaagc gaagcaaact gggaaagagt ccttacactg
     5041 gcctgcagtc cgtctcctcg gagaaggtgc ggtgcaagaa gagctgtggc caggccgagt
     5101 tgccatcctc ggtggcccac aaggtggccc agctgaagcc aaaggtcaag agcaaggggc
     5161 tgcctgccgg cctcggtgcc ttccagcgca aagaggctgc cccaggtggg cgcatccaga
     5221 agaagctatc tagagccaag agcgtcacgg cgtcaggggc ggcacggcat ccacaccccg
     5281 atggggacag tggcagggag atgcacaaat tccaagccca acccgcagtg gctgtggcac
     5341 atgaggcagg cagcggctat gacagtgagg actgccaggc actcctgggg acagaggcag
     5401 ctcccagaga gcctgggctg gtgttgcacc cagggtctgg tgtggcagtg ctgggaccct
     5461 caccttcctc cgtggtcaag atggaagcca atcagaaggc caagaagaaa aaggagaggc
     5521 agggcttgct aggggcctgc cgcctgtcca gccccgaggg cgaggttaag atcaagagac
     5581 gaacagtaaa gaccaaggtg ggagccaagc tggagcgggc gccagggcgg cggcccccgg
     5641 gtgcaccagg caagaagaag gccaagggca aggtgaaaac tggcctgcgt acagagccag
     5701 gaaccgccac cagcagagac acccttttca gtcccacccg gacttttgcc tgccgagagg
     5761 agggcagcaa acttgccagt gagcgcctca agagagccac ccgcaagagc gccatgttgc
     5821 agccagtcct gaggcggaag aatggggcct tgtccatcgc tctgtcagcc cgcaacgcca
     5881 aggccatcct gggaaagagc aggaagctga cgaaggtgaa gagagaggct gtcagcaagc
     5941 agggccaggg gcgggcggtg agccggctgc tggagagctt tgctgtggag gatgactttg
     6001 agtttgacga ggatgacacc agcttctcgg atgaggaaga agaagaggaa gaagccggtg
     6061 tacagctcag cgcagagcag agcgccgccc tggcacggtc ctgcaccatc cacaaggaag
     6121 acctgcagga cggactgccc gtcctgatcc cgaaggagga cagcctgctg tatgcgggca
     6181 gtgtcaggac cctgcaaccc cccgacatct acagcatcgt cattgagggg gagagaggca
     6241 accgtcaacg tatctactcg ctggaacagc tgctgcagga ggcggttctt gatgtccagc
     6301 cacagtccag tcggtacctt ccacctggta cccgggtctg tgcctactgg agtcagaagt
     6361 cccggtgtct atatccaggc aatgtggttc gaggtgcttc aagcgatgaa gaagacctgg
     6421 actcagtgtt ggtagagttc gatgacggag acacaggcca catagctgtc tctaacatca
     6481 ggctgctgcc tccggacttc aagatccagt gtacagagcc ctcgccagcc ctgttggtgt
     6541 ccagcagctg ccggaggacc aaaaaagcag ccaatgaggg ccacccgccc agcgaagccc
     6601 ccacacccag cctgtcccct aaagtgccgg atggccctga aacttctaag acccctggga
     6661 agaaatctgg cagcaaagac aaagctggca aagtcgacct cctaacctca ggtgccaagt
     6721 cccccacggg ggcctcagac cacttcctag gccgcagagg cagccccctg ctgagctggt
     6781 cagcagtggc tcagaccaag cggaaagcgg tggctgcggc agcggcggcg gcaggtggca
     6841 aagggccggg ggtactacag aacctcttcc agctcaacgg aagcaccaag aagctgcggg
     6901 cccgggacac cctgtttccc atgcatagca tggctacccc cgtgtttggt aacagcttcc
     6961 gagctgactc cttcagcagc ctggcgagtt cctacacacc cttccttgga ggggctgggg
     7021 caggccttcc cggaggagcc cacaagctgc ttagggccaa gaaggctgaa cgggctgaag
     7081 cagaaaaggc cgggaggcgg cgggcaggcg gcgagtttct ggttaagctg gaccatgagg
     7141 gtgtgacctc ccccaaaaac aaaaactgca aggccctgct catgagcgac aaggacttcg
     7201 gacccaagct ggggcgacct cttagcaacc ccagttacgc acacccagcc ctcattggca
     7261 aggacaagaa gggtcgggcg cctgtgcatc cgctacccat ggggctggct ctgcgcaagt
     7321 acccactgcc ctgtgatagt gactgcccca gctcctactc cgatgaggac gaggatgggc
     7381 cggggctggc caccggttat ccgtatgatg tgccggatta tgcgtaaacg cgtccggtcc
     7441 accaccacca ccaccactaa ggatccgggg ttggggttgc gccttttcca aggcagccct
     7501 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac
     7561 cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc
     7621 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg
     7681 ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac
     7741 ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc
     7801 tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt
     7861 ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg
     7921 gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagcaa
     7981 ttcaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     8041 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     8101 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     8161 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     8221 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     8281 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     8341 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     8401 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     8461 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     8521 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     8581 gccgaaggta gaggttctct cctcacttgt ggtgatgttg aagaaaaccc tggtccaatg
     8641 tctagactgg acaagagcaa agtcataaac ggagctctgg aattactcaa tggtgtcggt
     8701 atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg
     8761 tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac
     8821 aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac
     8881 aacgccaagt cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc
     8941 ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt
     9001 cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca
     9061 ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct
     9121 accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga ccggcaggga
     9181 gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta
     9241 aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca
     9301 gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt
     9361 gaccttgaca tgctccccgg gtaactaagt aaggatcgat ccaagatatc gtattcttaa
     9421 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     9481 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     9541 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     9601 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     9661 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     9721 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     9781 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     9841 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     9901 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     9961 tgtttcgcct cggcgtccgg actagaggta cctttaagac caatgactta caaggcagct
    10021 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
    10081 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
    10141 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
    10201 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
    10261 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
    10321 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
    10381 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    10441 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
    10501 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
    10561 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
    10621 agctattcca gaagtagtga ggaggctttt ttggaggcct ttcaaatatg tatccgctca
    10681 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    10741 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    10801 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    10861 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    10921 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
    10981 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    11041 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    11101 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    11161 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    11221 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    11281 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    11341 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    11401 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    11461 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    11521 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    11581 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    11641 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    11701 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    11761 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    11821 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    11881 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    11941 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    12001 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    12061 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    12121 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    12181 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    12241 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    12301 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    12361 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    12421 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    12481 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    12541 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    12601 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    12661 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    12721 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
    12781 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
    12841 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
    12901 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
    12961 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
    13021 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
    13081 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    13141 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    13201 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
    13261 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
    13321 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
    13381 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
    13441 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
    13501 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
    13561 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
    13621 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
    13681 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
    13741 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
    13801 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
    13861 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
    13921 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
    13981 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
    14041 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
    14101 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
    14161 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
    14221 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
    14281 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
    14341 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
    14401 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
    14461 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
    14521 gggac
//
