LOCUS       sequence-487457-        9347 bp    DNA     circular SYN 30-SEP-2025
DEFINITION  Expresses unspliced EPB41L4A-AS1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9347)
  AUTHORS   Monziani A,  Unfried JP, Cvetanovic T, Ulitsky I
  TITLE     EPB41L4A-AS1 long noncoding RNA acts in both cis- and trans-acting 
            transcriptional regulation and controls nucleolar biology
  JOURNAL   bioRxiv 2025.04.08.647803
REFERENCE   2  (bases 1 to 9347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 30, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.04.08.647803"
COMMENT     Sequence Label: pLV-Puro-CMV-EPB41L4A-AS1-unspliced MUT
FEATURES             Location/Qualifiers
     source          1..9347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2019..2322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2323..2526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2477..2497
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2547..2571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4020..4044)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4062..4650
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4115..4135)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4533..4544)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4681..5180
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4716..4739)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5113..5132
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5197..5796
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5197..5216)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5693..5713
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5868..6101
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6173..6294
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6210..6229)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6264..6283
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6334..6469
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6489..6508)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6490..6508)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6518..6535)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6518..6534)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6527..6549)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6676..7131
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6763..6782)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6973..6994
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7157..7261
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7262..7330,7331..8122)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7480..7499)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8293..8881
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8782..8801
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9035..9052
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9169..9190
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9205..9210,9211..9228,9229..9235)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9243..9259
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9248..9270
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9267..9283
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9267..9283
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9302..9322
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9304..9322
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgta gttattaata gtaatcaatt
     1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2521 agagctggtt tagtgaaccg tcagatccaa gtttgtacaa aaaagcaggc ttcctttctt
     2581 ccttttggtg cgagcttgct gtggtttttg ctctgggtcc tctgggatgg cgcctggctg
     2641 tggccgcgtg gtctctcacg caggggcgcc gggcggggga acgcggccac cctgagtctg
     2701 gtgagtcgac tgcggcggcc tgtgtccgaa gtgtccgggg ccgtgaacaa gggcagcggc
     2761 ctggcctcag gcctgcgttc ccacgtttgg aaacggggag cttcgtcgat ttgtgtttac
     2821 atcatcgact atgccaggga gttctccaga taagcctggt tttattttcg tcagtgaaaa
     2881 ggccttaccg tataactgac tttatgcttg ccctgccccc gtataaaata ccttagaagt
     2941 agagtgcctg gttacagctg tttccacgtg cggtgctcgt cgggagtgat cacctaccct
     3001 acaggtgagt tttcacgttc gtgcaagacc agtcgccatt taaaacgcat cgcatttcac
     3061 ttttcattat taagtcggat tttaaatcgt gagaaaattt ctctgaaatg tattgtccgt
     3121 ttttaggctg taatcggcat tactgtcagc cagtcagcaa ccttatgcca taaagcctac
     3181 ctcacgcagt gtcagccttt gtgttgccca ttcactttgg aaactagtga atgtggtgtc
     3241 aaaaaaggcg taaattaaac gctttgcagc cttttcctgc ccttaaattt gatacctttg
     3301 gtgtaggagc tgcataagta acagttgctg cttttacgtt tccacgcgtg atcttgaccc
     3361 tgctagcctt aagtgtatgg tttctcttag ccagttctaa tttttgttca ggtggaagat
     3421 ggatgcctga agtgtagact gctgctagct gaataccatc tgggagcata aaggtgacct
     3481 gaaggtaggg tgatatgtct taaagcactt tgtaatggga atttttatca ccttttaaat
     3541 tggggttcct tctctagtga gttttaatgt cagtggtaca ttcgtagtgt tgctctgtct
     3601 gtagctatta aggtgagtta ataaatggga tagcctccac agcttatttt tgggaaggtt
     3661 ttgctgatac ttcctgagaa gcccagggaa ataaatacgc atagtactgg cattctgcat
     3721 ctctttaaga tttgttttta tgtgtagtaa ttgagttttt taaaagcttg tgaaatcgca
     3781 ggcatattac caagttcttg attaaaatgt aatacaaaaa tatttgctgt cgaattgagt
     3841 actttatttt ttctcttagg atgtccttgg tgaggatttt gaaaatttga tcttcacaag
     3901 agttgcctgg atcatttgaa atttctggga gtctgaggag tactgacata attacctgct
     3961 ggagtctgta aatacacatt taagacagtg aggatgtgaa taaatatatt aatgcactta
     4021 cccagctttc ttgtacaaag tggtgataat cgaattccga taatcaacct ctggattaca
     4081 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4141 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4201 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4261 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4321 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4381 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4441 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     4501 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     4561 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     4621 gtcggatctc cctttgggcc gcctccccgc atcgggaatt cccgcggttc gaattctacc
     4681 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     4741 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     4801 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     4861 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     4921 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     4981 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     5041 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     5101 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     5161 cctcatctcc gggcctttcg acctcacgtg gccaccatga ccgagtacaa gcccacggtg
     5221 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     5281 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     5341 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     5401 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     5461 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     5521 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     5581 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     5641 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     5701 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     5761 cgcacctggt gcatgacccg caagcccggt gcctgaggta cctttaagac caatgactta
     5821 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5881 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     5941 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6001 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6061 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     6121 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     6181 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6241 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6301 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     6361 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     6421 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     6481 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     6541 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     6601 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     6661 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6721 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6781 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6841 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6901 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6961 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7021 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7081 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     7141 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7201 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7261 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7321 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7381 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7441 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7501 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7561 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7621 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7681 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7741 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7801 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7861 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7921 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7981 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     8041 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8101 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8161 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8221 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8281 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8341 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8401 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     8461 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8521 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8581 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8641 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8701 gcttcccgaa gagagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8761 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8821 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8881 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8941 attctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     9001 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     9061 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     9121 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     9181 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     9241 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     9301 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagctt
//
