LOCUS       sequence-487458-        9214 bp    DNA     circular SYN 30-SEP-2025
DEFINITION  Expresses unspliced EPB41L4A-AS1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Monziani A,  Unfried JP, Cvetanovic T, Ulitsky I
  TITLE     EPB41L4A-AS1 long noncoding RNA acts in both cis- and trans-acting 
            transcriptional regulation and controls nucleolar biology
  JOURNAL   bioRxiv 2025.04.08.647803
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 30, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.04.08.647803"
COMMENT     Sequence Label: pLV-Puro-CMV-EPB41L4A-AS1 unspliced-MUT-
            delta...
FEATURES             Location/Qualifiers
     source          1..9214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2019..2322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2323..2526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2477..2497
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2547..2571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3887..3911)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3929..4517
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3982..4002)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4400..4411)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4548..5047
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4583..4606)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4980..4999
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5064..5663
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5064..5083)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5560..5580
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5735..5968
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6040..6161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6077..6096)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6131..6150
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6201..6336
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6356..6375)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6357..6375)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6385..6402)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6385..6401)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6394..6416)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6543..6998
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6630..6649)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6840..6861
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7024..7128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7129..7197,7198..7989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7347..7366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8160..8748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8649..8668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8902..8919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9036..9057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9072..9077,9078..9095,9096..9102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9110..9126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9115..9137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9134..9150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9134..9150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9169..9189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9171..9189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgta gttattaata gtaatcaatt
     1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2521 agagctggtt tagtgaaccg tcagatccaa gtttgtacaa aaaagcaggc ttcctttctt
     2581 ccttttggtg cgagcttgct gtggtttttg ctctgggtcc tctgggatgg cgcctggctg
     2641 tggccgcgtg gtctctcacg caggggcgcc gggcggggga acgcggccac cctgagtctg
     2701 gtgagtcgac tgcggcggcc tgtgtccgaa gtgtccgggg ccgtgaacaa gggcagcggc
     2761 ctggcctcag gcctgcgttc ccacgtttgg aaacggggag cttcgtcgat ttgtgtttac
     2821 atcatcgact atgccaggga gttctccaga taagcctggt tttattttcg tcagtgaaaa
     2881 ggccttaccg tataactgac tttatgcttg ccctgccccc gtataaaata ccttagaagt
     2941 agagtgcctg gttacagctg tttccacgtg cggtgctcgt cgggagtgat cacctaccct
     3001 acaggtgagt tttcacgttc gtgcaagacc agtcgccatt taaaacgcat cgcatttcac
     3061 ttttcattat taagtcggat tttaaatcgt gagaaaattt ctctgaaatg tattgtccgt
     3121 ttttaggctg taatcggcat tactgtcagc cagtcagcaa ccttatgcca taaagcctac
     3181 ctcacgcagt gtcgctgctt ttacgtttcc acgcgtgatc ttgaccctgc tagccttaag
     3241 tgtatggttt ctcttagcca gttctaattt ttgttcaggt ggaagatgga tgcctgaagt
     3301 gtagactgct gctagctgaa taccatctgg gagcataaag gtgacctgaa ggtagggtga
     3361 tatgtcttaa agcactttgt aatgggaatt tttatcacct tttaaattgg ggttccttct
     3421 ctagtgagtt ttaatgtcag tggtacattc gtagtgttgc tctgtctgta gctattaagg
     3481 tgagttaata aatgggatag cctccacagc ttatttttgg gaaggttttg ctgatacttc
     3541 ctgagaagcc cagggaaata aatacgcata gtactggcat tctgcatctc tttaagattt
     3601 gtttttatgt gtagtaattg agttttttaa aagcttgtga aatcgcaggc atattaccaa
     3661 gttcttgatt aaaatgtaat acaaaaatat ttgctgtcga attgagtact ttattttttc
     3721 tcttaggatg tccttggtga ggattttgaa aatttgatct tcacaagagt tgcctggatc
     3781 atttgaaatt tctgggagtc tgaggagtac tgacataatt acctgctgga gtctgtaaat
     3841 acacatttaa gacagtgagg atgtgaataa atatattaat gcacttaccc agctttcttg
     3901 tacaaagtgg tgataatcga attccgataa tcaacctctg gattacaaaa tttgtgaaag
     3961 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     4021 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4081 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4141 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4201 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4261 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4321 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     4381 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     4441 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     4501 ttgggccgcc tccccgcatc gggaattccc gcggttcgaa ttctaccggg taggggaggc
     4561 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     4621 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     4681 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     4741 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     4801 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     4861 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     4921 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     4981 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     5041 cctttcgacc tcacgtggcc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     5101 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     5161 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     5221 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     5281 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     5341 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     5401 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     5461 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     5521 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     5581 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     5641 tgacccgcaa gcccggtgcc tgaggtacct ttaagaccaa tgacttacaa ggcagctgta
     5701 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5761 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     5821 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5881 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5941 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     6001 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     6061 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6121 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     6181 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6241 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6301 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     6361 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     6421 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     6481 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     6541 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6601 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6661 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6721 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     6781 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     6841 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     6901 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     6961 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     7021 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     7081 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     7141 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7201 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     7261 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     7321 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     7381 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     7441 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     7501 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7561 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7621 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     7681 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7741 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7801 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7861 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7921 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7981 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     8041 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     8101 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     8161 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     8221 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     8281 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     8341 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     8401 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     8461 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     8521 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaagag
     8581 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     8641 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     8701 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8761 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatatt ctttcctgcg
     8821 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8881 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     8941 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     9001 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     9061 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     9121 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     9181 cactaaaggg aacaaaagct ggagctgcaa gctt
//
