LOCUS       sequence-487484-        9688 bp    DNA     circular SYN 30-SEP-2025
DEFINITION  Secretory granule marker for endocrine cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9688)
  TITLE     Martin lab plasmids
REFERENCE   2  (bases 1 to 9688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 30, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NPY td-tomato
FEATURES             Location/Qualifiers
     source          1..9688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        263..466
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     417..437
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     463..487
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      817..826
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             823..2253
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     promoter        2281..2780
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2316..2339)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2713..2732
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2801..3400
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2801..2820)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3297..3317
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3414..4002
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3467..3487)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3885..3896)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4209..4842
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4971..4987)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4971..4987)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4984..5006)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4995..5011
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5019..5025,5026..5043,5044..5049))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5064..5085
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5202..5219)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5373..5961)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5453..5472)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6132..6923,6924..6992))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6755..6774
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6993..7097)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7145..7279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7182..7201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7236..7255
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7447..8080
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8127..8252
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8749..8982
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9167..9211
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9474..9589
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        join(9647..9688,1..262)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
       61 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      121 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      181 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      241 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      301 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      361 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      421 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
      481 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta
      541 ctagaggatc gctagcgcta ccggactcag atctcgagct caagcttggg ctgcaggtcc
      601 gcgccagcca ccatgctagg taacaagcga ctggggctgt ccggactgac cctcgccctg
      661 tccctgctcg tgtgcctggg tgcgctggcc gaggcgtacc cctccaagcc ggacaacccg
      721 ggcgaggacg caccagcgga ggacatggcc agatactact cggcgctgcg acactacatc
      781 aacctcatca ccaggcagag atatggaaaa ccggtcgcca ccatggtgag caagggcgag
      841 gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac
      901 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
      961 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
     1021 atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg
     1081 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg
     1141 accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc
     1201 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     1261 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg
     1321 aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag
     1381 cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac
     1441 gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg
     1501 gggcatggca ccggcagcac cggcagcggc agctccggca ccgcctcctc cgaggacaac
     1561 aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac
     1621 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     1681 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     1741 cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag
     1801 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt
     1861 ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag
     1921 atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg
     1981 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag
     2041 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc
     2101 aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc
     2161 cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg
     2221 ttcctgtacg gcatggacga gctgtacaag taagcggccg cgactctaga taattctacc
     2281 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     2341 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     2401 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     2461 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     2521 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     2581 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     2641 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     2701 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     2761 cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac
     2821 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     2881 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     2941 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     3001 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     3061 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     3121 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     3181 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     3241 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     3301 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     3361 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga ccgcgtctgg aacaatcaac
     3421 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3481 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3541 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3601 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3661 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3721 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3781 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     3841 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     3901 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     3961 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag
     4021 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     4081 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     4141 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     4201 gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     4261 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     4321 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     4381 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     4441 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     4501 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     4561 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     4621 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     4681 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     4741 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4801 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     4861 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac
     4921 attgctagcg ttttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     4981 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     5041 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     5101 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     5161 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     5221 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     5281 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     5341 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5401 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5461 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5521 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5581 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5641 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5701 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5761 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     5821 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5881 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5941 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6001 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6061 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     6121 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     6181 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     6241 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     6301 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     6361 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     6421 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     6481 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     6541 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6601 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6661 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     6721 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     6781 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6841 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6901 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6961 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     7021 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     7081 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     7141 gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7201 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7261 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
     7321 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
     7381 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
     7441 agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct
     7501 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
     7561 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
     7621 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
     7681 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
     7741 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
     7801 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
     7861 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
     7921 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7981 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     8041 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     8101 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     8161 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     8221 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     8281 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     8341 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     8401 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     8461 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     8521 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     8581 cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     8641 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     8701 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     8761 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     8821 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     8881 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     8941 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     9001 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     9061 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     9121 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     9181 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     9241 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     9301 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     9361 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     9421 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gcctttaaaa
     9481 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     9541 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     9601 acagggacag cagagatcca gtttatcgat aagcttggga gttccgcgtt acataactta
     9661 cggtaaatgg cccgcctggc tgaccgcc
//
