LOCUS       sequence-487492-        6178 bp    DNA     circular SYN 30-SEP-2025
DEFINITION  5' Gateway Entry vector containing the itga11a-cFos promoter for 
            fibroblast-specific expression in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6178)
  TITLE     Fibroblasts promote osmotic surveillance by wound-induced unique 
            calcium patterns
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 30, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p5E itga11a-cFos
FEATURES             Location/Qualifiers
     source          1..6178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        690..708
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3924..3940)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3924..3940)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(4119..4130)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        complement(4205..4223)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    4224..4347
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(4420..4438)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4443..4459)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4443..4459)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4561..4580)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4572..5381
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4639..4658)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5528..6116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6017..6036
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttggt aatacgactc actatagggc gaattgggta
      721 ccgaattgct ctgctttgat ggaaatattt gatgaaagga aagacagatg actttttaag
      781 tactcttaat gaatgcagcg ctctttactt tggtcatttt tgttttaagc aaaatcagct
      841 cagtgttgat cagatgtttg gtctctttgg tttcaaactt aaggcacata catagtagaa
      901 actgcaacat cctcatacta ttaagaattt atatattctt tattaatcgt gtgttcaata
      961 gtatgtaaca ttgatttttc aactcagatc ttcctccaca atttggtgga ttagttttct
     1021 ggcagtcaga aggtcgtgag ttctttaatg tgcttcttta atgttttatt gtttatctat
     1081 aatattgggt atcatggatt ttgagtatat aatggtacaa ataatgagat aaaaatgtat
     1141 ttttattgtt ttggacagtc ggaattccag acatgaataa gtgaatgatg ataattgaat
     1201 attattttcc tatacaatgg agataatgct tttaaaggta aaggaaatat ttaaatatct
     1261 gtggttaatt acagtatagt aaaaacgagt ttttaattat taagtggtcg tctaattaat
     1321 gacataatgt gattgacttt tgcacattgg aggaaggatg acactatggc tgaagtattt
     1381 cctgacaggt aatgttctgt tttaaaatta tttagtcacg caaagctgat gtggattgtg
     1441 ctgttttacg agtgagttat atgcacagaa gtgttgttca ccaacttaaa tcctccggca
     1501 aaacgttgat gtgactattt tcagtttcat aagggacctt ttacagcatc gacagattat
     1561 tatttagttt aacaacaaag catcagttaa tagagctatt cctccgagcc tgctttggtt
     1621 catttttgaa caatgacacc ctataatttt tatcatgcta ctttgaatat ttgatatgaa
     1681 atagcacgca agtatggctt agtaataagt gtaatttttg cacgccgcca gccaaccact
     1741 aactacattt ctagctggcg aatgacatta actaataggt tgtctgctgt catctttaag
     1801 gtgcgcacgt ttgtgtgcat gcaaaagcat ggctttggac agcaggggag ggactttgtt
     1861 ttaaagatat catgctagcc ggttagtatt ttggcatatc acctactaca cccttaatga
     1921 gtgctaatga tgaccttttg aatgataata ttaactatgt agattaacgc accaaaaatg
     1981 taaaaccgcg agccctgaac gtgatagttt tctgaaataa aatccatgtt tacaccaaag
     2041 atggttgcta taactatagt gatgacgata ttaacaatgc acaataatta aataatgtgc
     2101 tttaactact ataaaattca ttaatgaaat tgacctgggc gtcacggtag cgcaatgttt
     2161 agcacaatcg cctcacagta agaaggtcac tggttcgagc ctgggctggg tcagttggca
     2221 tttctgtctg gagattgcat gttctccccg tatttgcatg ctccagttta ccccacaagt
     2281 ccaaagacat gtggtatagg tgaattgaaa aggctaaatt gtccctagtg catgtgtgtg
     2341 aatgagtgtg catggatgtt taccattgat gggttgcaga tgaaaggaca taatctgcgt
     2401 aaaacatatg ctggataagt tggtggttca ttccactgtg tacagccttt aaagggatag
     2461 ttcacctaaa attgaaatat ctgtcatcgt ttactcaccc ttcacatata acaaaccagt
     2521 ttgagtttct tttttctgtt gaacacaaaa aagaagtgat tttgataaat ggaaacctgc
     2581 aaccattgac tcctatcgta tttgtttttc atactatggg tggttacaca tggttgcagg
     2641 tttctttaat ttatcatgta taatatatgt cttgtggcat ggtggctcag tggttagcac
     2701 tgttgcctca cagcaagaag gtcactgggt tgagtcccag ctgggccaga tagcatttct
     2761 gtgtggtgtt tgcatgttta ccctgtgttg gtgcgtgttt cccccggtgc tccggtttcc
     2821 cccacaattt aaagacatgt gatatagatg aattgaataa actaaattgg ccatatgagt
     2881 gtgtgaatga gagtgtatgg ctgtttcgca gtactgggtt gcagctagaa gggcattcgc
     2941 tgcgtaaaac acatgctgga atagttgctg gttcattcct ctgtcacaac ttataaaata
     3001 gagactaagc ttaaggaaaa tgaatgaatg aatatactgt atgtattttt aaatatatct
     3061 tctttgttca acacaaaaaa atgagctcca taaagatttg gaaacacttg agggtgagta
     3121 aataaacatt ttgtggtgaa atttgctttt aagtctgaca gttgtcttgt gaagtatata
     3181 aacaggatat tatgcatgtg tttggtagtt taattgtaag attacattta cactgcatgt
     3241 tcattttgac caaaaactaa gcaacagcgg ttttgatatt ttcaaggtca agttgaaatg
     3301 gaggtgggcc atgtttcact attagagcag ccagaacgtc agtattagtc attatataat
     3361 ctgtaattat atgcttgggt agcgtggttt ggatggtgtc tgaggttctg ctgtgagttt
     3421 gtcagtgggc tttatatggt aacttgcagc tacagtatgt taaccacaaa tctcggcatc
     3481 agtcaacttg gagggcatct tctccgaaag tatgcagcct aaaatgcatg ttaagcgaat
     3541 tacaaaatta cggaagatca ggttttgcca tagaggtgat atacagtgat ccctaaaacc
     3601 tgaatttcca tattcaaagc atttagcaat gagctcataa gaaagtgacc caaactccaa
     3661 cgtagatgga cagacacaca gagggaggaa gtaaaagaga gagaaagaaa aagagaaaag
     3721 atctgtctgc tggctgaatg agcacctcct tttggttcgg ccacagtccc ctcctccctg
     3781 cttcagatat agagtgtttt taggagcagt agagtcaatc agagccgggt gtacaggaga
     3841 gcgcactgtc aggatatttc atccttctgc acacacacac atacaccaac acacacattt
     3901 tcttacgttc tccctctcca acggatccac tagttctaga gcggccgcca ccgcggtggc
     3961 cagtgacgta ggaagtccat ccattcacag cgcttctata aaggcgccag ctgaggcgcc
     4021 tactactcca accgcgactg cagcgagcaa ctgagaagac tggatagagc cggcggttcc
     4081 gcgaacgagc agtgaccgcg ctcccaccca gctctgctct gcagctccca ccagtgtcta
     4141 cccctggacc ccttgccggg ctttccccaa acttcccacc gcggtggagc tccagctttt
     4201 gttcccttta gtgagggtta attcaagttt gtacaaaaaa gttgaacgag aaacgtaaaa
     4261 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
     4321 taaaacacaa catatgcagt cactatgaac caactactta gatggtatta gtgacctgta
     4381 gaattcgagc tctagagctg cagggcggcc gcgatatccc ctatagtgag tcgtattaca
     4441 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     4501 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     4561 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     4621 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     4681 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     4741 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     4801 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     4861 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     4921 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     4981 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     5041 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     5101 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     5161 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     5221 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     5281 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     5341 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     5401 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     5461 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5521 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5581 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5641 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5701 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5761 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5821 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5881 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5941 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6001 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6061 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6121 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
