LOCUS       sequence-487543-       10609 bp    DNA     circular SYN 30-SEP-2025
DEFINITION  Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of 
            Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces 
            beta-carotene in Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10609)
  AUTHORS   Cunningham FX Jr, Pogson B, Sun Z, McDonald KA, DellaPenna D, Gantt 
            E
  TITLE     Functional analysis of the beta and epsilon lycopene cyclase enzymes
            of Arabidopsis reveals a mechanism for control of cyclic carotenoid 
            formation.
  JOURNAL   Plant Cell. 1996 Sep;8(9):1613-26.
  PUBMED    8837512
REFERENCE   2  (bases 1 to 10609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 30, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Cell."; date: "1996-09"; volume: "8(9)"; pages: "1613-26"
COMMENT     Sequence Label: pAC-BETA
FEATURES             Location/Qualifiers
     source          1..10609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(928..947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1505..1510,1511..1527,1528..1533)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(8297..8316)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(join(10169..10609,1..219))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
     1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
     1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
     1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
     1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
     1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
     1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
     1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
     1861 ccgtcctgtg gatctaaagg cacagcgtct catgcttcgc acaatgtaaa actgcttcag
     1921 aacctggcga gagctatccg cgcggtctac ggttaactga tactaaaaga caattcagcg
     1981 ggtaaccttg caatggtgag tggcagtaaa gcgggcgttt cgcctcatcg cgaaatagaa
     2041 gtaatgagac aatccattga cgatcacctg gctggcctgt tacctgaaac cgacagccag
     2101 gatatcgtca gccttgcgat gcgtgaaggc gtcatggcac ccggtaaacg gatccgtccg
     2161 ctgctgatgc tgctggccgc ccgcgacctc cgctaccagg gcagtatgcc tacgctgctc
     2221 gatctcgcct gcgccgttga actgacccat accgcgtcgc tgatgctcga cgacatgccc
     2281 tgcatggaca acgccgagct gcgccgcggt cagcccacta cccacaaaaa atttggtgag
     2341 agcgtggcga tccttgcctc cgttgggctg ctctctaaag cctttggtct gatcgccgcc
     2401 accggcgatc tgccggggga gaggcgtgcc caggcggtca acgagctctc taccgccgtg
     2461 ggcgtgcagg gcctggtact ggggcagttt cgcgatctta acgatgccgc cctcgaccgt
     2521 acccctgacg ctatcctcag caccaaccac ctcaagaccg gcattctgtt cagcgcgatg
     2581 ctgcagatcg tcgccattgc ttccgcctcg tcgccgagca cgcgagagac gctgcacgcc
     2641 ttcgccctcg acttcggcca ggcgtttcaa ctgctggacg atctgcgtga cgatcacccg
     2701 gaaaccggta aagatcgcaa taaggacgcg ggaaaatcga cgctggtcaa ccggctgggc
     2761 gcagacgcgg cccggcaaaa gctgcgcgag catattgatt ccgccgacaa acacctcact
     2821 tttgcctgtc cgcagggcgg cgccatccga cagtttatgc atctgtggtt tggccatcac
     2881 cttgccgact ggtcaccggt catgaaaatc gcctgatacc gcccttttgg gttcaagcag
     2941 tacataacga tggaaccaca ttacaggagt agtgatgaat gaaggacgag cgccttgttc
     3001 agcgtaagaa cgatcatctg gatatcgttc tcgacccccg tcgcgccgta actcaggcta
     3061 gcgcaggttt tgagcgctgg cgctttaccc actgcgccct gccagagctg aattttagcg
     3121 acatcacgct ggaaaccacc ttcctgaatc ggcagctaca ggctccgctg ctgatcagct
     3181 ccatgaccgg cggcgttgag cgctcgcgcc atatcaaccg ccacctcgcc gaggcggcgc
     3241 aggtgctaaa aattgcgatg ggggtgggct cccagcgcgt cgccattgag agcgacgcgg
     3301 gcttagggct ggataaaacc ctgcggcagc tggctccgga cgtgccgctg ctggcgaacc
     3361 tcggcgcggc gcagctgacc ggcagaaaag gtattgatta cgcccgacgg gccgtggaga
     3421 tgatcgaggc ggatgcgctg attgtgcacc taaacccgct gcaggaggcg ctacagcccg
     3481 gcggcgatcg cgactggcgc ggacggctgg cggctattga aactctggtc cgcgagctgc
     3541 ccgttccgct ggtggtgaaa gaggtgggag ccggtatctc ccgaaccgtg gccgggcagc
     3601 tgatcgatgc cggcgttacc gtgattgacg tcgcgggcgc gggcggcacc agctgggccg
     3661 ccgttgaagg cgagcgggcg gccaccgagc agcagcgcag cgtggccaac gtctttgccg
     3721 actgggggat ccccaccgct gaggcgctgg ttgacattgc cgaggcctgg ccgcagatgc
     3781 cccttattgc ctcgggcggg attaaaaacg gcgtcgacca gccgcgctac gttgccgagg
     3841 ctaatctggt gatcacccac ggcggtctca ataccgtact ggatgcgctg gctgccgcga
     3901 cgccggtgct ggcggtgcca ctctctttcg accagcccgc cgtggctgcc cggctggtct
     3961 ataacgggct gggtcgccgg gtatcgcgct ttgccagaca gcagacgctg gcggatgaga
     4021 ttgcccaact gctgggggat gagacgctgc atcagcgtct ggcgacggcc cgccagcagc
     4081 ttaacgacgc cgggggcacg ccccgtgcgg cgaccctgat tgaacaggcc atagcaggga
     4141 gtgagagcgt atcgtgaggg atctgatttt agtcggcggc ggcctggcca acgggctgat
     4201 cgcctggcgt ctgcgccagc gctacccgca gcttaacctg ctgctgatcg aggccgggga
     4261 gcagcccggc gggaaccata cctggtcatt ccatgaagac gatctgactc ccgggcagca
     4321 cgcctggctg gccccgctgg tggcccacgc ctggccgggc tatgaggtgc agtttcccga
     4381 tcttcgccgt cgcctcgcgc gcggctacta ctccattacc tcagagcgct ttgccgaggc
     4441 cctgcatcag gcgctggggg agaacatctg gctaaactgt tcggtgagcg aggtgttacc
     4501 caatagcgtg cgccttgcca acggtgaggc gctgcttgcc ggagcggtga ttgacggacg
     4561 cggcgtgacc gccagttcgg cgatgcaaac cggctatcag ctctttcttg gtcagcagtg
     4621 gcggctgaca cagccccacg gcctgaccgt accgatcctg atggatgcca cggtggcgca
     4681 gcagcagggc tatcgctttg tctacacgct gccgctctcc gccgacacgc tgctgatcga
     4741 ggatacgcgc tacgccaatg tcccgcagcg tgatgataat gccctacgcc agacggttac
     4801 cgactatgct cacagcaaag ggtggcagct ggcccagctt gaacgcgagg agaccggctg
     4861 tctgccgatt accctggcgg gtgacatcca ggctctgtgg gccgatgcgc cgggcgtgcc
     4921 gcgctcggga atgcgggctg ggctatttca ccctaccact ggctattcgc tgccgctggc
     4981 ggtggccctt gccgacgcga ttgccgacag cccgcggctg ggcagcgttc cgctctatca
     5041 gctcacccgg cagtttgccg aacgccactg gcgcaggcag ggattcttcc gcctgctgaa
     5101 ccggatgctt ttcctggccg ggcgcgagga gaaccgctgg cgggtgatgc agcgctttta
     5161 tgggctgccg gagcccaccg tagagcgctt ttacgccggt cggctctctc tctttgataa
     5221 ggcccgcatt ttgacgggca agccaccggt tccgctgggc gaagcctggc gggcggcgct
     5281 gaaccatttt cctgacagac gagataaagg atgaaaaaaa ccgttgtgat tggcgcaggc
     5341 tttggtggcc tggcgctggc gattcgcctg caggcggcag ggatcccaac cgtactgctg
     5401 gagcagcggg acaagcccgg cggtcgggcc tacgtctggc atgaccaggg ctttaccttt
     5461 gacgccgggc cgacggtgat caccgatcct accgcgcttg aggcgctgtt caccctggcc
     5521 ggcaggcgca tggaggatta cgtcaggctg ctgccggtaa aacccttcta ccgactctgc
     5581 tgggagtccg ggaagaccct cgactatgct aacgacagcg ccgagcttga ggcgcagatt
     5641 acccagttca acccccgcga cgtcgagggc taccggcgct ttctggctta ctcccaggcg
     5701 gtattccagg agggatattt gcgcctcggc agcgtgccgt tcctctcttt tcgcgacatg
     5761 ctgcgcgccg ggccgcagct gcttaagctc caggcgtggc agagcgtcta ccagtcggtt
     5821 tcgcgcttta ttgaggatga gcatctgcgg caggccttct cgttccactc cctgctggta
     5881 ggcggcaacc ccttcaccac ctcgtccatc tacaccctga tccacgccct tgagcgggag
     5941 tggggggtct ggttccctga gggcggcacc ggggcgctgg tgaacggcat ggtgaagctg
     6001 tttaccgatc tgggcgggga gatcgaactc aacgcccggg tcgaagagct ggtggtggcc
     6061 gataaccgcg taagccaggt ccggctggcg gatggtcgga tctttgacac cgacgccgta
     6121 gcctcgaacg ctgacgtggt gaacacctat aaaaagctgc tcggccacca tccggtgggg
     6181 cagaagcggg cggcagcgct ggagcgcaag agcatgagca actcgctgtt tgtgctctac
     6241 ttcggcctga accagcctca ttcccagctg gcgcaccata ccatctgttt tggtccccgc
     6301 taccgggagc tgatcgacga gatctttacc ggcagcgcgc tggcggatga cttctcgctc
     6361 tacctgcact cgccctgcgt gaccgatccc tcgctcgcgc ctcccggctg cgccagcttc
     6421 tacgtgctgg ccccggtgcc gcatcttggc aacgcgccgc tggactgggc gcaggagggg
     6481 ccgaagctgc gcgaccgcat ctttgactac cttgaagagc gctatatgcc cggcctgcgt
     6541 agccagctgg tgacccagcg gatctttacc ccggcagact tccacgacac gctggatgcg
     6601 catctgggat cggccttctc catcgagccg ctgctgaccc aaagcgcctg gttccgcccg
     6661 cacaaccgcg acagcgacat tgccaacctc tacctggtgg gcgcaggtac tcaccctggg
     6721 gcgggcattc ctggcgtagt ggcctcggcg aaagccaccg ccagcctgat gattgaggat
     6781 ctgcaatgag ccaaccgccg ctgcttgacc acgccacgca gaccatggcc aacggctcga
     6841 aaagttttgc caccgctgcg aagctgttcg acccggccac ccgccgtagc gtgctgatgc
     6901 tctacacctg gtgccgccac tgcgatgacg tcattgacga ccagacccac ggcttcgcca
     6961 gcgaggccgc ggcggaggag gaggccaccc agcgcctggc ccggctgcgc acgctgaccc
     7021 tggcggcgtt tgaaggggcc gagatgcagg atccggcctt cgctgccttt caggaggtgg
     7081 cgctgaccca cggtattacg ccccgcatgg cgctcgatca cctcgacggc tttgcgatgg
     7141 acgtggctca gacccgctat gtcacctttg aggatacgct gcgctactgc tatcacgtgg
     7201 cgggcgtggt gggtctgatg atggccaggg tgatgggcgt gcgggatgag cgggtgctgg
     7261 atcgcgcctg cgatctgggg ctggccttcc agctgacgaa tatcgcccgg gatattattg
     7321 acgatgcggc tattgaccgc tgctatctgc ccgccgagtg gctgcaggat gccgggctga
     7381 ccccggagaa ctatgccgcg cgggagaatc gggccgcgct ggcgcgggtg gcggagcggc
     7441 ttattgatgc cgcagagccg tactacatct cctcccaggc cgggctacac gatctgccgc
     7501 cgcgctgcgc ctgggcgatc gccaccgccc gcagcgtcta ccgggagatc ggtattaagg
     7561 taaaagcggc gggaggcagc gcctgggatc gccgccagca caccagcaaa ggtgaaaaaa
     7621 ttgccatgct gatggcggca ccggggcagg ttattcgggc gaagacgacg agggtgacgc
     7681 cgcgtccggc cggtctttgg cagcgtcccg tttaggcggg cggccatgac gttcacgcag
     7741 gatcgcctgt aggtcggcag gcttgcgggc gtaaataaaa ccgaaggaga cgcagccctc
     7801 ccggccgcgc accgcgtggt gcaggcggtg ggcgacgtag agccgcttca ggtagccccg
     7861 gcgcgggatc ccgcctcgcc gtgctgtccg gtctcaacct gatccgccag aatcgagcca
     7921 acgggatcgg ccagcacgaa ttcggtatgc ggagagtgtt tggcaaacca tgcctgcaag
     7981 ccacccagcg tgccgccgga gccaacgcct actaccacgg catcaaccct gcctgccagc
     8041 tggtcgaaca gctccggtgc ggtggtggtg gcgtgcgcca gcgggttggc cgggttagag
     8101 aactgatcaa tatagtaagc acccggcgtc tcctctgcca ggcggcgggc gtagtcctga
     8161 tagtactccg ggtggccctt tgtcacgtcg gagcgggtca ggcgcacatc aacacccagc
     8221 gcacgcaggt ggtagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
     8281 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
     8341 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     8401 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     8461 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg
     8521 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     8581 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     8641 tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt
     8701 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa
     8761 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc
     8821 ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc
     8881 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat
     8941 cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat cattggaccg
     9001 ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt
     9061 gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg
     9121 gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa
     9181 ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca
     9241 tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc agcgttgggt
     9301 cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg
     9361 gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct
     9421 gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt
     9481 aaagtctgga aacgcggaag tcccctacgt gctgctgaag ttgcccgcaa cagagagtgg
     9541 aaccaaccgg tgataccacg atactatgac tgagagtcaa cgccatgagc ggcctcattt
     9601 cttattctga gttacaacag tccgcaccgc tgccggtagc tccttccggt gggcgcgggg
     9661 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     9721 gccggcagcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     9781 agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg
     9841 aacacctaca tctgtattaa cgaagcgcta accgttttta tcaggctctg ggaggcagaa
     9901 taaatgatca tatcgtcaat tattacctcc acggggagag cctgagcaaa ctggcctcag
     9961 gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt aaaccagcaa
    10021 tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc gaatttgctt
    10081 tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcaa ccaggcgttt
    10141 aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtact
    10201 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
    10261 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
    10321 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
    10381 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
    10441 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
    10501 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
    10561 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacg
//
