LOCUS       sequence-487557-        9787 bp    DNA     circular SYN 01-OCT-2025
DEFINITION  Expression of HA-TIR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  TITLE     Hengbin lab plasmids 2025
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PLVX-TIR1-puro
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2837..4561
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             4562..4588
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4562..4588
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4622..5121
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4657..4680)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5054..5073
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5142..5741
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5142..5161)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5638..5658
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5755..6343
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5808..5828)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6226..6237)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6550..7183
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7312..7328)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7312..7328)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7325..7347)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7336..7352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7360..7366,7367..7384,7385..7390))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7405..7426
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7543..7560)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7714..8302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7794..7813)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8473..9264,9265..9333))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9096..9115
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9334..9438)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9486..9620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9523..9542)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9577..9596
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ctcatcggga tccaggatga catactttcc tgaagaggtc gtcgaacaca tttttagctt
     2881 cctgcctgca cagagagata gaaacacagt gagcctggtc tgcaaagtgt ggtacgagat
     2941 cgaacgcctg agccggagag gagtgttcgt cggcaactgc tatgctgtga gagcaggcag
     3001 ggtcgccgct aggtttccaa atgtgcgcgc actgaccgtc aaggggaaac cccacttcgc
     3061 cgactttaac ctggtgcccc ctgattgggg aggatacgcc ggcccttgga tcgaggcagc
     3121 cgctcgcggc tgtcatggac tggaggaact gcgcatgaag cgaatggtgg tctctgacga
     3181 aagtctggag ctgctggctc ggagcttccc taggtttcgc gcactggtgc tgatttcttg
     3241 cgaaggcttc agcaccgatg gactggcagc cgtggcctcc cactgtaagc tgctgcggga
     3301 gctggacctc caggagaatg aagtggagga tagaggcccc agatggctgt cttgcttccc
     3361 agactcatgt accagcctgg tgtccctgaa ctttgcctgc atcaaaggcg aagtgaatgc
     3421 tgggtccctg gagcggctgg tctcaagaag ccccaacctg aggtctctgc ggctgaaccg
     3481 gagcgtgagc gtggacactc tggctaagat tctgctgaga acccctaacc tggaggatct
     3541 gggaaccggc aatctgacag acgatttcca gacagaatcc tactttaaac tgacttctgc
     3601 cctggagaag tgtaaaatgc tgaggagtct gtcaggattc tgggatgctt cacccgtgtg
     3661 cctgagcttt atctaccctc tgtgtgcaca gctgacaggc ctgaacctga gctatgcacc
     3721 aaccctggac gccagtgatc tgacaaagat gatctcacgc tgcgtgaaac tccagcgact
     3781 gtgggtgctg gactgtattt ccgataaggg gctccaggtg gtcgccagct cctgcaagga
     3841 cctccaggag ctgagagtgt tcccatctga tttttacgtg gccggatata gtgctgtcac
     3901 tgaggaaggc ctggtggcag tctcactggg atgcccaaag ctgaacagcc tgctgtattt
     3961 ctgtcatcag atgactaatg ctgcactggt gaccgtcgcc aagaactgcc ctaatttcac
     4021 ccgatttcgg ctgtgtattc tggaaccagg caaacccgac gtggtcacat cccagccact
     4081 ggatgaaggg tttggagcta tcgtgagaga gtgcaaggga ctccagaggc tgagcatttc
     4141 cggcctgctg acagacaaag tgttcatgta catcggcaag tatgctaagc agctggagat
     4201 gctgagcatt gcatttgccg gagactccga taagggcatg atgcacgtga tgaacgggtg
     4261 taagaatctg cgaaaactgg aaatccggga cagccctttc ggggatgccg ctctgctggg
     4321 aaactttgcc agatacgaga caatgaggag cctgtggatg tctagttgca atgtgactct
     4381 gaagggctgt caggtcctgg ctagtaaaat gcctatgctg aacgtggaag tcattaatga
     4441 gcgggacggg tctaacgaaa tggaggaaaa tcatggcgac ctgccaaagg tggagaaact
     4501 gtatgtgtat cggaccaccg caggggcaag agatgatgct cccaactttg tgaagattct
     4561 gtacccctac gacgtgcccg actacgcctg agaattccga catgctctag ataattctac
     4621 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     4681 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     4741 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     4801 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     4861 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     4921 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     4981 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     5041 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     5101 tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca
     5161 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     5221 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     5281 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     5341 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     5401 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     5461 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     5521 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     5581 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     5641 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     5701 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa
     5761 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5821 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5881 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5941 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6001 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6061 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6121 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     6181 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6241 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6301 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca
     6361 gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     6421 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     6481 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6541 agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     6601 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     6661 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     6721 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     6781 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     6841 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     6901 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     6961 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     7021 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7081 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7141 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     7201 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga
     7261 cattgctagc gttttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     7321 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     7381 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     7441 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7501 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7561 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7621 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7681 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7741 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7801 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7861 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7921 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7981 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8041 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8101 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     8161 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8221 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8281 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8341 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8401 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8461 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8521 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8581 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     8641 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8701 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8761 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8821 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8881 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8941 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9001 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9061 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9121 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9181 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9241 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9301 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9361 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9421 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     9481 agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     9541 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9601 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     9661 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
     9721 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     9781 tagtagt
//
