LOCUS       sequence-487560-       10439 bp    DNA     circular SYN 01-OCT-2025
DEFINITION  Expression of MLS-TIR1-P2A-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10439)
  TITLE     Hengbin lab plasmids 2025
REFERENCE   2  (bases 1 to 10439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MLS-TIR1-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..10439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..188
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    235..360
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    853..1086
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1271..1315
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1613..1730
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1781..2029
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1781..1801
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1952..1971
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2051..2126
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2209..2420
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2596..4320
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             4321..4347
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4357..4413
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4420..5130
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4565..4583)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4833..4852
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             5364..5597
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5623..5640)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5629..5853
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5899..6327
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5986..6005)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6196..6217
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6336..6356)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6341..6670
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6521..6656
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6583..6602
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6718..6765
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6784..7158
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7288..7409
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7325..7344)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7379..7398
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7458..7474)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7458..7474)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7471..7493)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7482..7498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7506..7512,7513..7530,7531..7536))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7551..7572
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7689..7706)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7860..8448)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7940..7959)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8619..9410,9411..9479))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9242..9261
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9480..9584)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9659..9678)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9850..10229
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10231..10429
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10384..10404
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
       61 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      121 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      181 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      241 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      301 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
      361 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
      421 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      481 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
      541 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
      601 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
      661 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
      721 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
      781 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
      841 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
      901 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
      961 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1021 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1081 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1141 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1201 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1261 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1321 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1381 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1441 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1501 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     1561 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     1621 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     1681 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     1741 acagggacag cagagatcca gtttggttaa ttaaggtacc gagggcctat ttcccatgat
     1801 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac
     1861 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     1921 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     1981 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg gcccaacatc
     2041 ctcgtgtcca gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     2101 ttgaaaaagt ggcaccgagt cggtgctctc cctgagcttc agggaggttt tttgaattcg
     2161 ctagctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca
     2221 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgatcc ggtgcctaga
     2281 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2341 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2401 ggtttgccgc cagaacacag gaccggttct agagccgcca ccatgttggg gtttgtgggt
     2461 cgggtggccg ctgctccggc ctccggggcc ttgcggagac tcaccccttc agcgtcgctg
     2521 cccccagctc agctcttact gcgggccgct ccgacggcgg tccatcctgt cagggactat
     2581 gcggcgcaaa gcgctatgac atactttcct gaagaggtcg tcgaacacat ttttagcttc
     2641 ctgcctgcac agagagatag aaacacagtg agcctggtct gcaaagtgtg gtacgagatc
     2701 gaacgcctga gccggagagg agtgttcgtc ggcaactgct atgctgtgag agcaggcagg
     2761 gtcgccgcta ggtttccaaa tgtgcgcgca ctgaccgtca aggggaaacc ccacttcgcc
     2821 gactttaacc tggtgccccc tgattgggga ggatacgccg gcccttggat cgaggcagcc
     2881 gctcgcggct gtcatggact ggaggaactg cgcatgaagc gaatggtggt ctctgacgaa
     2941 agtctggagc tgctggctcg gagcttccct aggtttcgcg cactggtgct gatttcttgc
     3001 gaaggcttca gcaccgatgg actggcagcc gtggcctccc actgtaagct gctgcgggag
     3061 ctggacctcc aggagaatga agtggaggat agaggcccca gatggctgtc ttgcttccca
     3121 gactcatgta ccagcctggt gtccctgaac tttgcctgca tcaaaggcga agtgaatgct
     3181 gggtccctgg agcggctggt ctcaagaagc cccaacctga ggtctctgcg gctgaaccgg
     3241 agcgtgagcg tggacactct ggctaagatt ctgctgagaa cccctaacct ggaggatctg
     3301 ggaaccggca atctgacaga cgatttccag acagaatcct actttaaact gacttctgcc
     3361 ctggagaagt gtaaaatgct gaggagtctg tcaggattct gggatgcttc acccgtgtgc
     3421 ctgagcttta tctaccctct gtgtgcacag ctgacaggcc tgaacctgag ctatgcacca
     3481 accctggacg ccagtgatct gacaaagatg atctcacgct gcgtgaaact ccagcgactg
     3541 tgggtgctgg actgtatttc cgataagggg ctccaggtgg tcgccagctc ctgcaaggac
     3601 ctccaggagc tgagagtgtt cccatctgat ttttacgtgg ccggatatag tgctgtcact
     3661 gaggaaggcc tggtggcagt ctcactggga tgcccaaagc tgaacagcct gctgtatttc
     3721 tgtcatcaga tgactaatgc tgcactggtg accgtcgcca agaactgccc taatttcacc
     3781 cgatttcggc tgtgtattct ggaaccaggc aaacccgacg tggtcacatc ccagccactg
     3841 gatgaagggt ttggagctat cgtgagagag tgcaagggac tccagaggct gagcatttcc
     3901 ggcctgctga cagacaaagt gttcatgtac atcggcaagt atgctaagca gctggagatg
     3961 ctgagcattg catttgccgg agactccgat aagggcatga tgcacgtgat gaacgggtgt
     4021 aagaatctgc gaaaactgga aatccgggac agccctttcg gggatgccgc tctgctggga
     4081 aactttgcca gatacgagac aatgaggagc ctgtggatgt ctagttgcaa tgtgactctg
     4141 aagggctgtc aggtcctggc tagtaaaatg cctatgctga acgtggaagt cattaatgag
     4201 cgggacgggt ctaacgaaat ggaggaaaat catggcgacc tgccaaaggt ggagaaactg
     4261 tatgtgtatc ggaccaccgc aggggcaaga gatgatgctc ccaactttgt gaagattctg
     4321 tacccatacg acgtcccaga ctacgctgga tccggcgcaa caaacttctc tctgctgaaa
     4381 caagccggag atgtcgaaga gaatcctgga ccgctcgaga tggtgagcaa gggcgaggag
     4441 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     4501 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     4561 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     4621 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     4681 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     4741 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     4801 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     4861 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     4921 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     4981 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     5041 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     5101 accggcggca tggacgagct gtacaagtag ggcgcgccgg ggtctttgtc ctctgtactg
     5161 tctctctcct tgcccctaac ccaaaaagct tcatttttct gtgtaggctg cacaagagcc
     5221 ttgattgaag atatattctt tctgaacagt atttaaggtt tccaataaaa tgtacacccc
     5281 tcagaacgta cggtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     5341 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     5401 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     5461 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     5521 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     5581 gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     5641 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     5701 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     5761 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     5821 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     5881 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     5941 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     6001 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     6061 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     6121 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     6181 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     6241 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     6301 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     6361 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6421 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6481 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     6541 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6601 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     6661 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     6721 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     6781 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     6841 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     6901 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     6961 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     7021 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     7081 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     7141 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     7201 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     7261 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     7321 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7381 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     7441 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7501 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7561 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7621 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7681 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7741 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7801 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7861 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7921 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7981 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8041 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8101 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8161 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8221 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8281 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8341 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8401 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8461 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8521 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8581 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8641 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8701 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8761 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8821 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8881 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8941 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9001 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9061 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9121 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9181 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9241 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9301 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9361 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9421 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9481 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9541 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9601 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     9661 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     9721 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     9781 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     9841 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     9901 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     9961 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
    10021 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
    10081 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
    10141 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
    10201 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
    10261 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
    10321 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
    10381 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgc
//
