LOCUS       sequence-487596-       11243 bp    DNA     circular SYN 01-OCT-2025
DEFINITION  Ae aegypti PUb promoter expressing barstar, and TRE expressing 
            barnase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11243)
  TITLE     Harnessing the highly adaptable barnase-barstar system for genetic 
            biocontrol of Aedes aegypti
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AGG1767 PUb-Bs-TRE-Bn
FEATURES             Location/Qualifiers
     source          1..11243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      541..1129
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1030..1049
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1283..1300
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1417..1438
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1453..1458,1459..1476,1477..1483)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1491..1507
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1496..1518
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1515..1531
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1515..1531
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   1619..1653
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    complement(1983..2016)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             3702..3725
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3738..3755
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     enhancer        4464..4946
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     regulatory      5780..5789
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5828..6502
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(6008..6028)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     6420..6443
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             6512..6532
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    7357..7390
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(7401..7434)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    join(7476..7494,7495..7517,7518..7536,7537..7559,7560..7578,
                     7579..7601,7602..7620,7621..7643,7644..7662,7663..7685,
                     7686..7704,7705..7727,7728..7746)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     CDS             8012..8053
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8534..8554
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    8648..8769
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8659..8678)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8713..8732
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8874..9569)
                     /codon_start=1
                     /product="Zoanthus yellow fluorescent protein"
                     /label=ZsYellow1
                     /note="mammalian codon-optimized"
                     /translation="MAHSKHGLKEEMTMKYHMEGCVNGHKFVITGEGIGYPFKGKQTIN
                     LCVIEGGPLPFSEDILSAGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWGRSFLFEDGAV
                     CICNVDITVSVKENCIYHKSIFNGVNFPADGPVMKKMTTNWEASCEKIMPVPKQGILKG
                     DVSMYLLLKDGGRYRCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKWQLTEH
                     AIAFPSALA"
     protein_bind    9805..9838
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   10031..10093
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10158..10175)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10158..10174)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10167..10189)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10383..10402)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10502..10524
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10562..10580)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10648..10752
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10753..10821,10822..11243,1..370)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10971..10990)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
       61 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
      121 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
      181 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
      241 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
      301 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
      361 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
      421 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
      481 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
      541 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
      601 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      661 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      721 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      781 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      841 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      901 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      961 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1021 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     1081 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     1141 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     1201 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     1261 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     1321 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1381 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     1441 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     1501 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg catgcaggcc
     1561 tctgcagtcg acgggcccgg gatccgatat cccaattata gggagagcgg ccgcttaacc
     1621 ctagaaagat agtctgcgta aaattgacgc atgcattctt gaaatattgc tctctctttc
     1681 taaatagcgc gaatccgtcg ctgtgcattt aggacatctc agtcgccgct tggagctccc
     1741 gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt gaactataac gaccgcgtga
     1801 gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca tacgataatt
     1861 atattgttat ttcatgttct acttacgtga taacttatta tatatatatt ttcttgttat
     1921 agatatccct agagtagtgc cccaactggg gtaacctttg agttctctca gttgggggcg
     1981 taataacttc gtatagtata ccttatacga agttatgcta gcagcgatcg ctatctttac
     2041 atgtagcttg tgcattgaat ccaattataa tttgccttgg caccagctga gccagacaag
     2101 aaagaaagct tcccagaagt atatcgattt agaagggttg acgtcacttg ctgactgcac
     2161 taatacagca aatgatgcaa ttagaatgat tcaagtgaaa ttcccaaatt actgattttt
     2221 ctctggattt ggttatcaga ttacattcga agctaagatt agctaccgaa attgtcgatc
     2281 aaatcaggaa atcctttctc tatcgaaaaa ggcattcgca catcttcctc tctatgccat
     2341 atacacgaag ggtaggtaca ttgacgtctt tgccagaagt tgaactgcat cgttcaaggt
     2401 acagaatgaa cgactaacag acacaagcac gttttgctgt ccattcagac acagggatgg
     2461 tacccatatt cgatcgatat agagccatcc aaccgaacag aggtatatgt atgaatgtat
     2521 tgctgaaatt ttctagaagt acaaccacca ctacgacagt gtctataaaa cgcccctgca
     2581 aaggcgaaac cagctcaatc gaatacgttt cctagtggag tgaacattac gcggcccaag
     2641 taagcagtgc cagtgcaagt gaagtgaagt ctctagtgaa aaagagtgat ccaattagcc
     2701 agaggagaaa atttcagagt gaacaaagct ttattcaaag gacaattact attaaattgg
     2761 tgaaagtgca tttcggtgaa gggaatcttc tagtgaaggt aggtaaatta attgatgaaa
     2821 ttatagctat gagcgaaaag tagtttggtg aatgattcct ttgtctttga atgagcaaac
     2881 tattttccaa gatggcgact attgagcttt gagtgattag tgaaaatttg caacgcagtt
     2941 tcatcatcat tgataaaacc caattgtgat tcacagcgat aatcatattt cgttgaatca
     3001 tcgctactaa ttgaattaaa tttctagaat aataagaata acgtatttgc tccgtcacat
     3061 atctaaaata aatattttga tggtaattac ccattaaggt aatattaaca catatcgaga
     3121 aaaaccttga ggaaatcgtg aaaacttgaa gatacgcaat ttcaaaacta cgtagttcaa
     3181 agtcgaaaac aagttaattt ttcacttaaa agtagggcgt tgttgtgacg tcatcacctt
     3241 caagtgtata tttttcactt ggcctgcgac tgcaaacgca gacaaagcaa aacaagttta
     3301 aaacctgtcg tgtcgtgctc gaagccaaag gcaatgaatc aatatcaaat gagagtttgc
     3361 atttcacaac caattactca agcgtttcct cgtttctttt tctgctcaac agagatttca
     3421 acatgaagaa ggccgtgatc aacggagaac agatccgttc gatctcggat ctgcaccaga
     3481 ccctgaagaa ggaactggcc ctgccggaat actacggaga aaacctggat gccctgtggg
     3541 atgccctgac cggatgggtg gaatacccgc tggtgctgga atggcggcag ttcgaacagt
     3601 cgaagcagct gaccgaaaac ggagccgaat cggtgctgca ggtgttccgt gaagccaagg
     3661 ccgaaggagc cgatatcacc atcatcctgt cgggtgccgg agactacaaa gatgacgatg
     3721 acaagagcgg cgccggacac catcaccacc atcattaact agctgacact acgtgatgaa
     3781 tcgtttttaa aataacaaat caattgtttt ataatattcg tacgattctt tgattatgta
     3841 ataaaatgtg atcattagga agattacgaa aaatataaaa aatatgagtt ctgtgtgtat
     3901 aacaaatgct gtaaacgcca caattgtgtt tgttgcaaat aaacccatga ttatttgatt
     3961 aaaattgttg ttttctttgt tcatagacaa tagtgtgttt tgcctaaacg tgtactgcat
     4021 aaactccatg cgagtgtata gcgagctagt ggctaacgct tgccccacca aagtagattc
     4081 gtcaaaatcc tcaatttcat caccctcctc caagtttaac atttggccgt cggaattaac
     4141 ttctaaagat gccacataat ctaataaatg aaatagagat tcaaacgtgg cgtcatcgtc
     4201 cgtttcgacc atttccgaaa agaactcggg cataaactct atgatttctc tggacgtggt
     4261 gttgtcgaaa ctctcaaagt acgcagtcag gaacgtgcgc gacatgtcgt cgggaaactc
     4321 gcgcggaaac atgttgttgt aaccgaacgg gtcccatagc gccaaaacca aatctgccag
     4381 cgtcaataga atgagcacga tgccgacaat ggagctggct tggatagcga ttcgagttaa
     4441 caagctttac gagtagaatt ctacgcgtaa aacacaatca agtatgagtc ataatctgat
     4501 gtcatgtttt gtacacggct cataaccgaa ctggctttac gagtagaatt ctacttgtaa
     4561 tgcacgatca gtggatgatg tcatttgttt ttcaaatcga gatgatgtca tgttttgcac
     4621 acggctcata aactcgcttt acgagtagaa ttctacgtgt aacgcacgat cgattgatga
     4681 gtcatttgtt ttgcaatatg atatcataca atatgactca tttgtttttc aaaaccgaac
     4741 ttgatttacg ggtagaattc tacttgtaaa gcacaatcaa aaagatgatg tcatttgttt
     4801 ttcaaaactg aactcgcttt acgagtagaa ttctacgtgt aaaacacaat caagaaatga
     4861 tgtcatttgt tataaaaata aaagctgatg tcatgttttg cacatggctc ataactaaac
     4921 tcgctttacg ggtagaattc tacgcgtaaa acatgattga taattaaata attcatttgc
     4981 aagctatacg ttaaatcaaa cggacgctcg aggttgcaca acactattat cgatttgcag
     5041 ttcgggacat aaatgtttaa atatatcgat gtctttgtga tgcgcgcgac atttttgtag
     5101 gttattgata aaatgaacgg atacgttgcc cgacattatc attaaatcct tggcgtagaa
     5161 tttgtcgggt ccattgtccg tgtgcgctag tagcatgccc gtaacggacc tcgtactttt
     5221 ggcttcaaag gttttgcgca cagacaaaat gtgccacact tgcagctctg catgtgtgcg
     5281 cgttaccaca aatcccaacg gcgcagtgta cttgttgtat gcaaataaat ctcgataaag
     5341 gcgcggcgcg cgaatgcagc tgatcacgta cgctcctcgt gttccgttca aggacggtgt
     5401 tatcgacctc agattaatgt ttatcggccg actgttttcg tatccgctca ccaaacgcgt
     5461 ttttgcatta acattgtatg tcggcggatg ttctatatct aatttgaata aataaacgat
     5521 aaccgcgttg gttttagagg gcataataaa agaaatattg ttatcgtgtt cgccattagg
     5581 gcagtataaa ttgacgttca tgttggatat tgtttcagtt gcaagttgac actggcggcg
     5641 acaagcaatt ggtacccggg taggatccta gtgaattcct aatctggcgg taagttgatc
     5701 aaaggaaacg caaagttttc aagaaaaaac aaaactaatt tgatttataa cacctttaga
     5761 aagcgaagtt gagattcagg ccaccatggg agatcccacc ccacccaaga agaagcgcaa
     5821 agctagcatg gcctcctccg agaacgtcat caccgagttc atgcgcttca aggttcgcat
     5881 ggagggcacc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     5941 cgagggccac aacaccgtga agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     6001 ggacatcctg tccccccagt tccagtacgg ctccaaggtc tacgtgaagc accccgccga
     6061 catccccgac tacaagaagc tgtccttccc ggaaggattc aagtgggagc gcgtgatgaa
     6121 cttcgaggac ggcggcgtgg ctaccgtgac ccaggactcc tccctgcaag acggctgctt
     6181 catctacaag gttaagttca tcggcgtgaa cttcccctcc gacggccccg tgatgcagaa
     6241 gaagacaatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
     6301 gggcgagacc cacaaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa
     6361 gtccatctac atggccaaga agcccgtgca gctgcccggc tactactacg tggacgccaa
     6421 gctggacatc acctcccaca acgaggacta caccatcgtg gagcagtacg agcgcaccga
     6481 gggccgccac cacctgttcc tgagatctcg acccaagaaa aagcggaagg tggaggaccc
     6541 gtaagatcca ccggatctag ataactggag cttgataaca ttatacctaa acccatggtc
     6601 aagagtaaac atttctgcct ttgaagttga gaacacaatt aagcatcccc tggttaaacc
     6661 tgacattcat acttgttaat agcgccataa acatagcacc aatttcgaag aaatcagtta
     6721 aaagcaatta gcaattagca attagcaata actctgctga cttcaaaacg agaagagttg
     6781 caagtatttg taaggcacag tttatagacc accgacggct cattagggct cgtcatgtaa
     6841 ctaagcgcgg tgaaacccaa ttgaacatat agtggaatta ttattatcaa tggggaagat
     6901 ttaaccctca ggtagcaaag taatttaatt gcaaatagag agtcctaaga ctaaataata
     6961 tatttaaaaa tctggccctt tgaccttgct tgtcaggtgc atttgggttc aatcgtaagt
     7021 tgcttctata taaacacttt ccccatcccc gcaataatga agaataccgc agaataaaga
     7081 gagatttgca acaaaaaata aaggcattgc gaaaactttt tatgggggat cattacactc
     7141 gggcctacgg ttacaattcc cagccactta agcgacaagt ttggccaaca atccatctaa
     7201 tagctaatag cgcaatcact ggtaatcgca agagtatata ggcaatagaa cccatggatt
     7261 tgaccaaagg taaccgagac aatggagaag caagaggatt tcaaactgaa cacccacagt
     7321 actgtgtact accactggcg cgtttggggg ccggccataa cttcgtataa tgtatgctat
     7381 acgaagttat gacacggaaa ataacttcgt atagtatacc ttatacgaag ttatggcgcg
     7441 ccctcgagcg cgcctcggaa ttcgactttc acttttctct atcactgata gggagtggta
     7501 aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt
     7561 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga
     7621 gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc
     7681 acttttctct atcactgata gggagtggta aactcgactt tcacttttct ctatcactga
     7741 tagggagtgg taaactcgaa aacgagcgcc ggagtataaa tagaggcgct tcgtctacgg
     7801 agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat
     7861 aaacaagcgc agctgaacaa gctaaacaat ctgcggtacc ctggcgtcta attggggtaa
     7921 gttttcccgt tcttttctgg gttcttccct tttgctcatc cttgctgcac taccttcagg
     7981 tgcaagtaaa gcccgcggtg ggactagcat gggaaagccg atcccgaacc cgctgctggg
     8041 actggattcg accggtagcg gagccggcag tggtgccggc tccggagccg gttcgggagc
     8101 cggcagcggt gccggctccg gaaccgccca ggttatcaac accttcgatg gagtggccga
     8161 ttacctgcag acctaccaca agctgccgga taactacatc accaagtcgg aagcccaggc
     8221 cctgggatgg gtggcctcga agggaaacct ggccgatgtg gccccgggaa agtcgatcgg
     8281 aggagatatc ttctcgaacc gtgaaggaaa gctgccggga aagtcgggac gtacctggcg
     8341 tgaagtaaga ggagatcttg actaagtaga tttatggcgc aacgctatca caggaacata
     8401 actgacataa tctagacgcg ttactaactg gcacctcttc tttttttttg atatcctgca
     8461 ggccgatatc aactacacct cgggattccg taactcggat cgtatcctgt actcgtcggg
     8521 tggaagcggc agtgaaaacc tgtacttcca gggatcggga tcgggaaccg gagccggatc
     8581 gtcggattgg ctgatctaca agaccaccga tgcctaccag accttcacca agatccgtta
     8641 acctagttaa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     8701 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     8761 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     8821 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcaggcc
     8881 agggcgctgg ggaaggcgat ggcgtgctcg gtcagctgcc acttctggtt cttggcgtcg
     8941 ctccggtcct cccgcagcag cttgtgctgg atgaagtgcc actcgggcat cttgctgggc
     9001 acgctcttgg ccttgtacac ggtgtcgaac tggcaccggt accggccgcc gtccttcagc
     9061 agcaggtaca tgctgacatc gcccttcagg atgccctgct taggcacggg catgatcttc
     9121 tcgcagctgg cctcccagtt ggtggtcatc ttcttcatca cggggccgtc ggcggggaag
     9181 ttcacgccgt tgaagatgct cttgtggtag atgcagttct ccttcacgct cacggtgatg
     9241 tccacgttac agatgcacac ggcgccgtcc tcgaacagga agctccggcc ccaggtgtag
     9301 ccggcggggc agctgttctt gaagtagtcc acgatgtcct gggggtactc ggtgaagatc
     9361 cggtcgccgt acttgaagcc ggcgctcagg atgtcctcgc tgaagggcag ggggccgccc
     9421 tcgatcacgc acaggttgat ggtctgcttg cccttgaagg ggtagccgat gccctcgccg
     9481 gtgatcacga acttgtggcc gttcacgcag ccctccatgt ggtacttcat ggtcatctcc
     9541 tccttcaggc cgtgcttgct gtgggccatt cgactctagc ggtaccccga ttgtttagct
     9601 tgttcagctg cgcttgttta tttgcttagc tttcgcttag cgacgtgttc actttgcttg
     9661 tttgaattga attgtcgctc cgtagacgaa gcgcctctat ttatactccg gcggtcgagg
     9721 gttcgaaatc gataagcttg gatcctaatt gaattagctc taattgaatt agtctctaat
     9781 tgaattagat ccccgggccc taggataact tcgtataatg tatgctatac gaagttatga
     9841 ctcacgttaa ttcgataaaa gttttgttac tttatagaag aaattttgag tttttgtttt
     9901 tttttaataa ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag
     9961 tgtaaatata ataaaactta atatctattc aaattaataa ataaacctcg atatacagac
    10021 cgataaaaca catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat
    10081 tatctttcta gggttaacca tggaaaatcg atgattggga tatctagatg cattcgcgag
    10141 gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    10201 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    10261 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
    10321 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
    10381 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
    10441 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
    10501 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
    10561 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
    10621 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    10681 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    10741 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    10801 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    10861 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    10921 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    10981 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    11041 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    11101 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    11161 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    11221 gtaactcgcc ttgatcgttg gga
//
