LOCUS       sequence-487614-        8490 bp    DNA     circular SYN 01-OCT-2025
DEFINITION  shRNA knockdown human OGDH gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8490)
  TITLE     Zhu Lab plasmids 2025
REFERENCE   2  (bases 1 to 8490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti-shOGDH #1
FEATURES             Location/Qualifiers
     source          1..8490
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(188..199)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             354..587
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    659..780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(696..715)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     750..769
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      820..955
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(975..994)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(976..994)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1004..1021)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1004..1020)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1013..1035)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1162..1617
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1249..1268)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1459..1480
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1643..1747
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1748..1816,1817..2608)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1966..1985)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2779..3367
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3268..3287
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3521..3538
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(3691..3696,3697..3714,3715..3721)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3729..3745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3734..3756
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3753..3769
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3753..3769
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3788..3808
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3790..3808
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3836..4062
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4063..4243
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4290..4415
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4908..5141
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5326..5370
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5668..5916
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5668..5688
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5839..5858
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    6013..6130
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6253..6464
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6477..6745
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      6791..6800
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6821..7483
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             7553..7606
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7607..8206
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7607..7626)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8103..8123
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(8260..8280)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
       61 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
      121 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
      181 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
      241 gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg atggtacctt taagaccaat
      301 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
      361 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
      421 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      481 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      541 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
      601 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
      661 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
      721 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
      781 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
      841 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
      901 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
      961 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     1021 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     1081 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     1141 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     1201 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     1261 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     1321 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     1381 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     1441 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     1501 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     1561 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     1621 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1681 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1741 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     1801 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1861 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     1921 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     1981 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     2041 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     2101 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     2161 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     2221 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     2281 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     2341 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     2401 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2461 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2521 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2581 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2641 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     2701 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2761 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2821 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2881 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     2941 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3001 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3061 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3121 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3181 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3241 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3301 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3361 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3421 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3481 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     3541 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     3601 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     3661 gagttagctt actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     3721 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     3781 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     3841 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     3901 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     3961 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     4021 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     4081 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     4141 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     4201 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     4261 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     4321 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     4381 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     4441 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     4501 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     4561 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     4621 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     4681 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     4741 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     4801 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     4861 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     4921 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     4981 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     5041 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     5101 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     5161 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     5221 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     5281 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     5341 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     5401 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     5461 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     5521 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     5581 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg atcacgagac
     5641 tagcctcgag cggccgcccc cttcaccgag ggcctatttc ccatgattcc ttcatatttg
     5701 catatacgat acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag
     5761 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     5821 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     5881 ttggctttat atatcttgtg gaaaggacga aacaccggcc tgagtatgag gaggaaattc
     5941 tcgagaattt cctcctcata ctcaggtttt tgaattctcg acctcgagac aaatggcagt
     6001 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     6061 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     6121 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc ggctcgaggg
     6181 ggttggggtt gcgccttttc caaggcagcc ctggctagcc cgggatctgc gatcgctccg
     6241 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     6301 tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     6361 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     6421 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg
     6481 ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga
     6541 gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa
     6601 gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac
     6661 tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt
     6721 tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacacta gtctagagga
     6781 tccgctagac gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg
     6841 ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac
     6901 ccccaagcag ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag
     6961 cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag
     7021 cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat
     7081 cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg
     7141 ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt
     7201 caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa
     7261 cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt
     7321 cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg
     7381 gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat
     7441 cgtggagtac cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc
     7501 gtccaattct gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag
     7561 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa
     7621 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     7681 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     7741 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     7801 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     7861 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     7921 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     7981 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     8041 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     8101 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     8161 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga
     8221 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     8281 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     8341 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     8401 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     8461 caccacctgt cagctccttt ccgggacttt
//
